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Citations for Addgene :
5301 - 5350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... a gift from Raffaele De Francesco (Addgene plasmid # 155296; http://n2t.net/addgene:155296; RRID: Addgene 155296) (38) ...
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bioRxiv - Molecular Biology 2023Quote: ... which had been produced in HEK293T cells by transiently co-transfecting the lentiviral packaging plasmid psPAX2 (Addgene #12260), a lentiviral vector encoding μ-OR-SmBiT (pLenti-Blast Addgene #17451) ...
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bioRxiv - Molecular Biology 2023Quote: ... full length BRCA1 was PCR-amplified from pCL-MFG-BRCA1 (Addgene #12341 (Ruffner and Verma, 1997)) with a triple Ty1-tag included in the reverse primer and subsenquently cloned into pENTR_1A and transferred to pCW57.1 using gateway cloning ...
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bioRxiv - Molecular Biology 2023Quote: ... EXO1 was PCR amplified from pTXB1-EXO1b (Addgene #68267 (El-Shemerly et al., 2005)) and transferred directly into pCW57.1 using Gibson Assembly.
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bioRxiv - Molecular Biology 2023Quote: ... we adapted the plentiGuide-puro vector (Addgene plasmid # 52963)51 to incorporate a second guide RNA expression cassette as described previously52 ...
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bioRxiv - Molecular Biology 2023Quote: ... pGFPGUSPlus was a gift from Claudia Vickers (Addgene plasmid # 64401 ...
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bioRxiv - Molecular Biology 2023Quote: ... An AAV that only expressed the mCherry protein under the same neuronal promoter was use as control condition (Addgene Plasmid #114472). Wildtype and Cdr1as-KO neurons were infected at DIV4-6 by directly adding the viral particles into the culturing media at a titer of 109 VG/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... For plasmids obtain from Addgene, same procedure of propagation and plasmid purification was used.
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bioRxiv - Molecular Biology 2023Quote: The sequence of pri-miR-7a was amplified from mouse brain tissue and cloned into an AAV vector (Addgene Plasmid #99126), driven by a neuronal promoter (hSyn1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The previously described pSLI-TGD plasmid (Addgene #85791, Birnbaum et al., 2017) was modified by inserting a recodonized version of TurboID (“V4” ...
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: Bacterial expression of CP plasmid was purchased from Addgene (Plasmid #89950). The α subunit of CP was subcloned into a pRSFDuet-based vector in frame with an N terminal His-Sumo tag and the β subunit of CP was subcloned into a pCDFuet-based vector ORF2 ...
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bioRxiv - Molecular Biology 2023Quote: ... pRSV-Rev (Addgene plasmid 12253), and psPAX2 (Addgene 12260 ...
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bioRxiv - Molecular Biology 2023Quote: ... values were converted into copy numbers by measuring against a standard curve of the AAV transfer plasmid or the Ai9 plasmid (a gift from H. Zeng Addgene plasmid # 22799). The AAV genome copy number was divided by the number of nuclei to normalize for tissue input.
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bioRxiv - Molecular Biology 2023Quote: ... AAV helper (AAV9 (a gift of J. M. Wilson Addgene, plasmid # 112867), AAVMYO (7) ...
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bioRxiv - Molecular Biology 2023Quote: ... The shRNAs used were either purchased from Addgene (shCtrl: Addgene #1864) or designed using the Genetic Perturbation Platform.
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bioRxiv - Molecular Biology 2023Quote: ... HeLa cells were generated by infecting cells with lentivirus containing a Cas9-P2A-GFP expression cassette under a doxycycline inducible promoter as described previously (Addgene plasmid #85400)50 ...
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bioRxiv - Molecular Biology 2023Quote: ... The shRNAs used were either purchased from Addgene (shCtrl: Addgene #1864) or designed using the Genetic Perturbation Platform.
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bioRxiv - Molecular Biology 2023Quote: ... sgRNA libraries cloned into pMCB320 (Addgene #101926 ...
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bioRxiv - Molecular Biology 2023Quote: Stimulated emission depletion microscopy was performed on U2OS cells transduced with lentivirus expressing Flag-tagged PREPL cDNA (pCIG3, Addgene #78264). Cells were seeded 3 days post-transduction on coverslips (thickness #1.5H ...
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bioRxiv - Molecular Biology 2023Quote: K562 cells (ATCC) were transduced with lentiCas9-Blast (Addgene #52962) and single cell clones were selected for stable Cas9 expression ...
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bioRxiv - Molecular Biology 2023Quote: CRISPR mediated mutagenesis of target genes was performed by transducing K562 cells stably expressing Cas9 (lentiCas9-Blast, Addgene #52962) with lentivirus expressing sgRNAs (lentiGuide-Puro ...
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bioRxiv - Molecular Biology 2023Quote: ... or a control MYC-mito tag (Addgene #83355) as previously described37 ...
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bioRxiv - Molecular Biology 2023Quote: ... with lentivirus expressing sgRNAs (lentiGuide-Puro, Addgene #52963) targeting genes of interest ...
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bioRxiv - Molecular Biology 2023Quote: Mitochondrial immunoprecipitations were performed using K562 cells expressing an HA-mito tag (Addgene #83356) or a control MYC-mito tag (Addgene #83355 ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid pEGFP-GR (Addgene #47504, gift from Alice Wong) was used to express GR-EGFP ...
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bioRxiv - Molecular Biology 2023Quote: ... a gift from Douglas Kim & GENIE Project) and the same titer of Cre (AAV9-hSyn-Cre-WPRE-hGH; Addgene #105553 ...
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bioRxiv - Molecular Biology 2023Quote: ... before using LR reaction to transfer the cDNA into destination vectors pLEX_305-C-dTAG or pLEX_305-N-dTAG (Addgene #91797 & #91798 ...
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bioRxiv - Molecular Biology 2023Quote: ... and pEGFP-N1-IL6 (Addgene #111933 ...
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bioRxiv - Neuroscience 2023Quote: ... and mice were injected with either the AAV5-DIO-hTyr experimental virus or a comparable AAV5-DIO-EYFP control virus (Addgene, plasmid #27056). Due to the volume of virus required for these experiments ...
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bioRxiv - Neuroscience 2023Quote: Cre-dependent recombinant adeno-associated virus (rAAV) expressing GCaMP7f under the control of the Synapsin promoter (rAAV1-Syn-FLEX-jGCaMP7f-WPRE-Sv40, Addgene #104492, titer: 1 × 1013 vg/mL) was used to express GCaMP7f in interneurons.
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bioRxiv - Neuroscience 2023Quote: ... Each AAV-PHP.eB capsid carried a plasmid for jGCaMP7s driven by the synapsin promoter and WPRE regulatory element (AAV-PHP.eB-Synapsin-jGCaMP7s-WPRE; Addgene # 104487-PHPeB). Virus injections took less than 10 minutes from the beginning of anesthesia induction to full recovery ...
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bioRxiv - Neuroscience 2023Quote: ... Then postBirA* fragment and pAAV-FLEX-GFP vector (Addgene, Cat # 28304) were both digested with KpnI (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... we injected AAV1-CAG-Flex-TdTomato (400nl, Addgene, Cat.#: 28306) into L2 of M2 in Vipr2-Cre mice ...
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bioRxiv - Neuroscience 2023Quote: ... AAV1-CAG-Flex-TdTomato (400nl, Addgene, Cat.#: 28306) for Vipr2-Cre mice were used for virus injection ...
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bioRxiv - Neuroscience 2023Quote: We used the pLKO.1 vector (Addgene plasmid 10878)7 for expression of shRNA against rat Sirt3 (target ...
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bioRxiv - Neuroscience 2023Quote: ... A human Sirt3-RFP driven by the CaMKII promoter was constructed through PCR of the human Sirt3 sequence from Sirt3-FLAG (Addgene plasmid 13814)5 into the BamHI and AgeI sites of CaMKII-MICU3-RFP plasmid6 resulting in the linker sequence RPVVA joining Sirt3 and RFP sequences.
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bioRxiv - Neuroscience 2023Quote: ... AAV8-hSyn-mCherry (Addgene, 114472-AAV8), and AAV9-Syn-GCaMP6m-WPRE-SV40 (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... AAV8-hSyn-DIO-Gi–mCherry (Addgene, 44362-AAV8), AAV8-hSyn-mCherry (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... AAV1-hSYN1::Cre (Addgene, #105553), AAV1-hSYN1::ChrimsonR-tdTomato (56 ...
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bioRxiv - Neuroscience 2023Quote: ... AAV1-hSYN1::ChrimsonR-tdTomato (56) (Addgene, #59171-AAV1) and AAV-DJ-hSYN1::GCaMP6f (Stanford University Neuroscience Gene Vector and Virus Core ...
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bioRxiv - Neuroscience 2023Quote: ... 150nl of AAV2-hSyn-DIO-hM3D(Gq)-mcherry or 150-600nl of AAV2-hSyn-DIO-mCherry (3×1012 vg/ml, 5.1×1012 vg/ml and 5.6×1012 vg/ml, respectively; Addgene and UNC Vector Core) into the MeA of Foxp2cre+/- mice ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV2/9-pAAV-hDlx-hM4Di-dTomato-Fishell-5 (Addgene, 83896) at a concentration of 6+13 genome copies per ml (gc/ml) ...
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bioRxiv - Neuroscience 2023Quote: ... we generated pAAV-GFAP-GCaMP6m plasmid from flexed-GCaMP6 and pZac2.1-GfaABC1D-mCherry−hPMCA2w/b (Addgene, 111568) and used AAV2/9-pAAV-GFAPGCaMP6m at a concentration of 1.07527E+13 genome copies per ml (gc/ml) ...
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bioRxiv - Neuroscience 2023Quote: ... paavCAG-pre-mGRASP-mCerulean and paavCAG-post-mGRASP-2A-dTomato from Addgene (Cat #, 53581, 34910 and 34912, RRID: Addgene_53581, Addgene_34910 and Addgene_34912). Overlap extension PCR was used to construct BirA* probes (Fig ...
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bioRxiv - Neuroscience 2023Quote: ... paavCAG-pre-mGRASP-mCerulean and paavCAG-post-mGRASP-2A-dTomato from Addgene (Cat # ...
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bioRxiv - Neuroscience 2023Quote: ... we used AAV2/9-pAAV-hDlx-hM3Dq-dTomato-Fishell-4 (Addgene, 83897) and AAV2/9-pAAV-hDlx-hM4Di-dTomato-Fishell-5 (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... paavCAG-pre-mGRASP-mCerulean and paavCAG-post-mGRASP-2A-dTomato from Addgene (Cat #, 53581, 34910 and 34912, RRID: Addgene_53581, Addgene_34910 and Addgene_34912). Overlap extension PCR was used to construct BirA* probes (Fig ...
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bioRxiv - Neuroscience 2023Quote: ... Each pair of sgRNAs was cloned into pAAV-U6-sgRNA-CMV-GFP (Addgene, #85451) by using the SapI restriction enzyme (BioLabs ...
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bioRxiv - Neuroscience 2023Quote: ... paavCAG-pre-mGRASP-mCerulean and paavCAG-post-mGRASP-2A-dTomato from Addgene (Cat #, 53581, 34910 and 34912, RRID: Addgene_53581, Addgene_34910 and Addgene_34912). Overlap extension PCR was used to construct BirA* probes (Fig ...
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bioRxiv - Neuroscience 2023Quote: ... Uchida) or 120nl of mixed AAV9-Ef1a-fDIO-Cre (2.5×1013 vg/ml; Addgene) and AAV2-CAG-Flex-GCaMP6f (1:2 ...