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Citations for Addgene :
5251 - 5300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... pCIG3N (N-tropic MLV gag-pol from Addgene) and pLXIN-GFP were used with Env to produce virus ...
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bioRxiv - Microbiology 2024Quote: ... and Ochrobactrum MYb14 expressing GFP from a high-copy broad host range plasmid (pBTK519, KmR, Addgene #110603) (Leonard et al ...
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bioRxiv - Microbiology 2024Quote: ... YE(NGN)– YB(KOW)) were cloned into a pTYB2 backbone (Addgene catalog N6702S) after PCR amplification from Bacteroides fragilis ATCC 25285 (NCTC 9343 ...
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bioRxiv - Molecular Biology 2024Quote: HCT116 cells (eIF6-WT and eIF6-N106S) were transfected with 4µg of pcDNA-firefly-luciferase plasmid DNA (Addgene; 18964) and 20 µL of Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... pMD2.G (Addgene #12259-Didier Trono Lab). For silencing of Hop ...
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bioRxiv - Microbiology 2024Quote: ... psPAX2 (Addgene #12260-Didier Trono Lab) and the VSV-G envelope expressing plasmid ...
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bioRxiv - Microbiology 2024Quote: ... pGIPZ-shHOP or pGIPZ-shNT (Addgene) containing either shRNA targeting human HOP or a non-targeting control sequence ...
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bioRxiv - Microbiology 2024Quote: ... in the lentiviral pLJM1-eGFP backbone (Addgene #19319).
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bioRxiv - Cell Biology 2024Quote: ... mEmerald-γ-tubulin was from Addgene #54105 ...
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bioRxiv - Cancer Biology 2024Quote: ... pLenti CMV rtTA3 Hygro (w785-1) (Addgene: 26730), pCW57.1-4EBP1_4xAla (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... pCW57.1-4EBP1_4xAla (Addgene: 38240).
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bioRxiv - Cancer Biology 2024Quote: 5’ UTR elements or part of the GAPDH gene body102 were cloned into the dual luciferase assay construct (Addgene:45642). 1.5 million cells/6cm plate were transfected with 2μg of the construct using lipofectamine 2000 at a ratio of 3:1 lipofectamine to μg DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... 293T cells were plated in 10cm plates and transfected using lipofectamine 2000 with 6μg lentiviral pCW57.1-4EBP1_4xAla (Addgene: 38240), 1.5μg pMD2G and 4.5μg psPax2 ...
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bioRxiv - Cell Biology 2024Quote: ... and pLenti-CRISPRvs-puro (Plasmid 98291, Addgene; target = GCAGCACCGAAGCTTCGTTG-AGG). Lentivirus was generated in HEK293 cells and placed directly on fibroblasts ...
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bioRxiv - Cell Biology 2024Quote: ... two lentiviral plasmids targeting Myh9 were constructed: pLenti-CRISPRvs-puro (Plasmid 98290, Addgene; target = ACCCTGCATCATGCTCCGGT-AGG) and pLenti-CRISPRvs-puro (Plasmid 98291 ...
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bioRxiv - Cell Biology 2024Quote: ... RPE1-iCas9 cells were transfected with Cas9-2A-EGFP (pX458; Addgene #48138) containing a gRNA against CSB ...
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bioRxiv - Cell Biology 2024Quote: ... and pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL60 (Addgene). These FAP sequences and the pcDNA3.1 backbone were used for generating the FAP-GFP-KDEL ...
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bioRxiv - Cell Biology 2024Quote: ... cells were co-transformed with pET28a plasmid encoding the amber mutant TTC5 and the pEVOL-pBpF plasmid (Addgene plasmid #31190). Cells were grown at 37 °C in LB containing 50 μg/mL kanamycin and 25 μg/mL chloramphenicol and induced with 0.2 % L-arabinose at an A600 of 0.3 for 30 min followed by a second induction with 0.2 mM IPTG at an A600 of ∼0.6 at 16 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... VC-TUBA1B/TUBB-P2A-VN-TTC5 constructs were synthetized by Twist Bioscience (used Venus sequence from Addgene plasmid #10580453) and TTC5 mutants generated by site-directed mutagenesis ...
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Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: ... The REX-Shh-promoter::lacZ construct was created by introducing the REX element into the PCR4-Shh-promoter::lacZ-H11 vector (Addgene Plasmid #139098) (Kvon et al. ...
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bioRxiv - Bioengineering 2024Quote: ... In-Fusion cloning (TakaraBio) was used to separately clone the different pegRNAs into the piggyBac plasmid vector (Addgene #173222), which contains the prime editor (PE2) ...
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bioRxiv - Biochemistry 2024Quote: Human Drosha isoform 4 and human DGCR8 clones were purchased from Addgene. cDNA encoding different length variants of Drosha were cloned into pFL plasmid and expressed as a N-terminal Dual-strep tag fusion ...
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bioRxiv - Biochemistry 2024Quote: ... which was a kind gift from Laszlo Hunyady (Addgene Plasmid # 164784), together with AgeI and XhoI restriction sites ...
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bioRxiv - Biophysics 2024Quote: Dictyostelium discoideum strain HM3478 used in this work is derived from Ax2(Kay) [31] by transformation [32] with the plasmid pPI304 (available from Addgene under the reference 113232) expressing reporters for PIP3 and actin (PH-PkbEeGFP ...
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bioRxiv - Synthetic Biology 2024Quote: ... OmpA-TaSil silicatein was inserted into vector pBbS5a-RFP (56) (Addgene #35283) replacing the vector’s RFP gene ...
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bioRxiv - Synthetic Biology 2024Quote: ... pCAT20115 was purchased from Addgene (#134878) and used as a backbone for further plasmid construction ...
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bioRxiv - Molecular Biology 2024Quote: ... pMD2.G construct (Addgene #12259), and the respective lentiviral transfer plasmid ...
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bioRxiv - Molecular Biology 2024Quote: Non-replicating lentiviruses were produced in HEK293T cells using the 2nd generation packaging plasmids psPAX2 construct (Addgene #12260), pMD2.G construct (Addgene #12259) ...
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bioRxiv - Synthetic Biology 2024Quote: ... B2AR were acquired from Addgene. Plasmid sources for CCR6 ...
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bioRxiv - Synthetic Biology 2024Quote: ... pYFAC (Addgene ID# 168982)(Chooi et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... sgRNA oligos were cloned into pX330 (Addgene #42230), which also expresses Cas9 ...
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bioRxiv - Synthetic Biology 2024Quote: ... B2103: pMOD_B2013 (#91061, Addgene) for CRISPR knockout and activation systems in plants ...
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bioRxiv - Synthetic Biology 2024Quote: A0502: pMOD_A0502 (#91012, Addgene) for CRISPR knockout system in N ...
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bioRxiv - Synthetic Biology 2024Quote: ... where we use the OptoCre-VVD2 variant (Addgene #160400). The original FHY1 and ΔPhyA plasmids were constructed using the red light domains developed in Zhou et al.38 ...
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bioRxiv - Synthetic Biology 2024Quote: ... We then insert a separate promoter and reporter cassette (minP, luciferase) excerpted from pMPRAdonor2 (Addgene #49353) in between the enhancer and barcode elements ...
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bioRxiv - Synthetic Biology 2024Quote: ... adding the appropriate spacer sequence into the pSQT1313 vector (Addgene #53370)62 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and CMV-VPR-ZF37-BGH (Addgene plasmid #138839). PCR fragments of Addgene plasmids #138759 and #138730 were used to clone target gene pPVs for VP16-ZF37 and VP16-ZF43 through Gibson assembly ...
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bioRxiv - Synthetic Biology 2024Quote: ... CMV-VP64-ZF43-BGH (Addgene plasmid #138835), and CMV-VPR-ZF37-BGH (Addgene plasmid #138839) ...
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bioRxiv - Synthetic Biology 2024Quote: ... VCre from CAG-VCre-RbG (Addgene plasmid #89575), GIB-Cre N-terminal (Addgene plasmid #108723) ...
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bioRxiv - Synthetic Biology 2024Quote: ... recombinase was expressed as: Cre from CAG-Cre-RbG (Addgene plasmid #89573), VCre from CAG-VCre-RbG (Addgene plasmid #89575) ...
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bioRxiv - Synthetic Biology 2024Quote: ... GIB-Cre N-terminal (Addgene plasmid #108723), and GIB-Cre C-terminal (Addgene plasmid #108724 ...
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bioRxiv - Synthetic Biology 2024Quote: ... CMV-VP64-ZF37-BGH (Addgene plasmid #138834), CMV-VP64-ZF43-BGH (Addgene plasmid #138835) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and GIB-Cre C-terminal (Addgene plasmid #108724) (gifts from the Wong Lab at Boston University) ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids pHEE401E and pCBC-DT1T2 were gifted by Qi-Jun Chen (Wang et□al.□2015, Addgene plasmid #71287; Xing et al. 2014, Addgene plasmid #50590). All binary and subcloning vector series generated in this study will be deposited in Addgene (https://www.addgene.org/).
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bioRxiv - Plant Biology 2024Quote: ... The MoClo Plant Parts Kit was a gift from Nicola Patron (Addgene kit # 1000000047) (Engler et al ...
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bioRxiv - Plant Biology 2024Quote: ... and a CaMV 35S terminator (pICH41414; Addgene catalog # 50337). The screenable marker RUBY (He et al ...
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bioRxiv - Plant Biology 2024Quote: ... with a CaMV 35S short promoter (pICH51277; Addgene catalog # 50268), and a CaMV 35S terminator (pICH41414 ...
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bioRxiv - Molecular Biology 2023Quote: ... a gift from Raffaele De Francesco (Addgene plasmid # 155296 ...
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bioRxiv - Molecular Biology 2023Quote: ... AAVMYO2 (8) or AAVMYO3 (8)) and pAdDeltaF6 helper (a gift from J. M. Wilson Addgene, plasmid # 112867) plasmid using PEI MAX (Polyscience) ...
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bioRxiv - Molecular Biology 2023Quote: ... the genome editing one vector system (PX459) (104142, Addgene, MA, USA) was used ...