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Citations for Addgene :
5201 - 5250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... We then digested this fragment with the SbfI and AscI restriction enzymes and ligated with a fragment from the pRS415_pGal-nCas9(D10A) plasmid (Addgene #79618) digested with the same pair of enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... and pCMV-VSV-G (10 μg, Addgene #8454) along with a pLenti6.2 expression plasmid (10 μg ...
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bioRxiv - Molecular Biology 2024Quote: ... pDONR207 SARS-CoV-2 nsp1 Delta RC (M1, K164A/H165A, Addgene #164522) and pDONR207 SARS-CoV-2 nsp1 R124A/K125A (M2 ...
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bioRxiv - Molecular Biology 2024Quote: ... nsp1 coding sequences were amplified by PCR from pDONR207 SARS-CoV-2 nsp1 (WT, Addgene #141255), pDONR207 SARS-CoV-2 nsp1 Delta RC (M1 ...
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bioRxiv - Molecular Biology 2024Quote: ... and pDONR207 SARS-CoV-2 nsp1 R124A/K125A (M2, Addgene #164523) plasmids using the primers 5-EX-NSP1 and 3-NB-NSP1 and cloned into pEGFP-N1 digested with EcoRI and NotI enzymes ...
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bioRxiv - Molecular Biology 2024Quote: PSuperPuroRetro-1660s(hPOT1)39 (a gift from Christopher Counter, Addgene #11136) was used as a scramble control in both experiments.
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bioRxiv - Molecular Biology 2024Quote: ... a gift by Titia de Lange (Addgene #18002, Addgene #18008), were used to produce lentiviral vectors in HEK 293T cells ...
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bioRxiv - Molecular Biology 2024Quote: ... we used PSuperPuroRetro-1660(hPOT1) (a gift from Christopher Counter, Addgene #11135)39 ...
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bioRxiv - Molecular Biology 2024Quote: pMSP1D1 (Addgene plasmid #20061 ...
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bioRxiv - Molecular Biology 2024Quote: ... and pMSP2N2 (Addgene plasmid #29520; http://n2t.net/addgene:29520; RRID:Addgene_29520) plasmids were a gift from the Sligar lab 29,30,40,41 ...
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bioRxiv - Molecular Biology 2024Quote: ... Kozak sequence was added to RCaMP1h by PCR using RCaMP1h cDNA from pRSET-RCaMP1h plasmid (Addgene plasmid #42874) as the template ...
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bioRxiv - Molecular Biology 2024Quote: ... The sensing domain for citrate comprised residues 4–133 of the CitAP domain from Klebsiella pneumoniae CitA protein (Addgene plasmid #134301). The sensing domain for glucose comprised residues 24–328 of the bacterial D-galactose-binding periplasmic protein (MglB ...
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bioRxiv - Microbiology 2024Quote: The TurboID-V5 gene sequence was obtained from AddGene (#107167). The AgrD1-32 domain consists of an N-terminal domain from amino acid 1-27 and the AIP region from amino acid 28-32 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were co-transfected with 0.25 µg of HA-D1R and 0.25 µg of β-arrestin2-GFP (plasmid # 35411, Addgene, Watertown, MA) in 10 µL of lipofectamine2000 per well as directed by the manufacturer’s protocol and incubated overnight ...
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bioRxiv - Microbiology 2024Quote: ... pCMV-HA-USP18 was generated through subcloning using Flag-HA-USP18 (Addgene #22572; a gift from Wade Harper (87)) as a template.
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bioRxiv - Microbiology 2024Quote: ... pcDNA-V5-NSP3 was generated by amplifying the full-length NSP3 ORF from pDONR207 SARS-CoV-2 NSP3 (Addgene #141257; a gift from Fritz Roth (86)) and by subcloning it into the pcDNA3-C-V5 vector ...
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bioRxiv - Microbiology 2024Quote: ... The gam-gfp was placed under the control of the Tetracycline induction system (Addgene, pFD152, #125546). Similarly ...
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bioRxiv - Microbiology 2024Quote: ... or pTRIPZ (gift from Sandra Demaria – Addgene #127696) vectors using In-Fusion cloning (Takara) ...
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bioRxiv - Microbiology 2024Quote: CMT-93 cells expressing mCherry were created by transfection with pMCB320 (a gift from Michael Bassik, Addgene plasmid #89359 ...
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bioRxiv - Microbiology 2024Quote: ... and psPAX2 (a gift from Didier Trono, Addgene plasmid #12260 ...
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bioRxiv - Microbiology 2024Quote: ... pCMV-VSV-G (a gift from Bob Weinberg, Addgene plasmid #8454, http://n2t.net/addgene:8454, RRID:Addgene_8454), and psPAX2 (a gift from Didier Trono ...
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bioRxiv - Microbiology 2024Quote: ... pCMV-VSV-G (a gift from Bob Weinberg, Addgene plasmid #8454 ...
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bioRxiv - Microbiology 2024Quote: ... 293β5 cells [56] were transfected with LentiCRISPR v2 (a gift from Feng Zhang, Addgene plasmid #52961 ...
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bioRxiv - Microbiology 2024Quote: ... 293β5 cells [56] were transfected with LentiCRISPR v2 (a gift from Feng Zhang, Addgene plasmid #52961, http://n2t.net/addgene:52961, RRID:Addgene_52961), pCMV-VSV-G (a gift from Bob Weinberg ...
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bioRxiv - Microbiology 2024Quote: CMT-93 cells expressing mCherry were created by transfection with pMCB320 (a gift from Michael Bassik, Addgene plasmid #89359, http://n2t.net/addgene:89359, RRID:Addgene_89359) using Lipofectamine 2000 ...
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bioRxiv - Microbiology 2024Quote: ... and psPAX2 (a gift from Didier Trono, Addgene plasmid #12260, http://n2t.net/addgene:12260, RRID:Addgene_12260) using Lipofectime 2000 (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... the full-length ZBP1 gene was cloned into the PGAMA lentiviral expression vector (Addgene #74755). This vector co-expresses mCherry ...
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bioRxiv - Neuroscience 2024Quote: ... pro-lentiviral vector1 containing a 476 bp human synapsin-1 promoter element driving the neuronal specific expression of HA-tagged SpCas9 flanked by the bipartite SV40 nuclear localization signal (NLS) HA-NLS-SpCas9-NLS (from Addgene, plasmid #131497) at AgeI and BsrGI sites followed by woodchuck hepatitis post-transcriptional regulatory element (WPRE ...
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bioRxiv - Neuroscience 2024Quote: ... into our AAV template backbone (Addgene #196041) at BamHI and HindIII cloning sites ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV hSyn INTRON HaloTag-Jph3 WPRE was generated replacing mCherry with HaloTag (from Addgene #123285) at BglII and HindIII sites ...
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bioRxiv - Neuroscience 2024Quote: ... a gift from Keith Joung (Addgene plasmid # 43860; http://n2t.net/addgene:43860; RRID:Addgene_43860). sgRNAs were tested for efficacy in HEK293 (ATCC ...
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bioRxiv - Neuroscience 2024Quote: ... a gift from Keith Joung (Addgene plasmid # 43860 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with a plasmid expressing ACTN2 (NM_001103.4) with a eGFP tag.19 The plasmid was a gift from Johannes Hell (Addgene plasmid ref #52669). For immunoprecipitation of RBM20 ...
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bioRxiv - Neuroscience 2024Quote: ... or the Cre-dependent Gi/o-coupled DREADD vector (AAV8-hSyn-DIO-hM4d-mCherry, Addgene, Watertown, MA) into the CeA (AP −1.06 ...
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bioRxiv - Developmental Biology 2024Quote: ... pCMV-SPORT6-hHMGCR1 was a gift from Anne Galy (Addgene plasmid # 86085; http://n2t.net/addgene:86085; RRID:Addgene_86085). Mutagenic primers harboring the desired variants were used in a PCR reaction with wild type human HMGCR sequence as the template ...
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bioRxiv - Developmental Biology 2024Quote: ... pCMV-SPORT6-hHMGCR1 was a gift from Anne Galy (Addgene plasmid # 86085 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We obtained plasmids from Addgene and transformed the pCas9-CR4 (ID ...
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bioRxiv - Genetics 2024Quote: pCDF5-U6-[4xgRNA-tRNA]-GFP was generated by cloning the 4 gRNAs targeting GFP from (Ma et al., 2016) in pCDF5 (Addgene #Plasmid #73914) (Port and Bullock ...
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bioRxiv - Genetics 2024Quote: AID12* and AID123* or EvolvR (Addgene #83260) were kind gifts from Rahul Kohli (University of Pennsylvania ...
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bioRxiv - Genetics 2024Quote: pattB-Actin5C-BE2(pActin5c-CD-dCas9m4-UDI-attB) was obtained from Addgene# 104879 (Marr and Potter ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and psPAX2 (gift from Didier Trono, Addgene plasmid # 12260), into HEK293T cells with a 1:1:1 stoichiometry ...
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bioRxiv - Neuroscience 2024Quote: ... Gi DREADD virus (n=25,13 males, 12 females: AAV8-hSyn-DIO-hM4Di-mCherry,≥ 1×101 3 vg/mL, Addgene; Watertown, MA, USA) was mixed with GAD1-cre to express inhibitory designer receptors in VP GABA neurons ...
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bioRxiv - Neuroscience 2024Quote: Cre-dependent recombinant adeno-associated virus (rAAV) for GCaMP7f (rAAV1-syn-FLEX-jGCaMP7f-WPRE, Addgene #1944820-AAV1, titer: ≥1:1013 vg/mL) were used to express GCaMP7f in the hippocampus of Vgat-Cre mice ...
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bioRxiv - Genomics 2024Quote: ... The oligo library was cloned into the double digested AgeI/SbfI pLS-SceI vector (Addgene,137725) using the NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs ...
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bioRxiv - Molecular Biology 2024Quote: ... pLentiCRISPR v2 plasmid was obtained from Addgene (52961). pCMV-Myc was purchased from Clontech.
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bioRxiv - Molecular Biology 2024Quote: A PMET17 C-ubiquitin RURA3 plasmid (GC408; Addgene 131163, [56]) was linearized with SalI directly upstream of the C-ubiquitin sequence and gap-repaired with PCR products amplified from the OY235 yeast genome ...
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bioRxiv - Molecular Biology 2024Quote: ... The ppyCAG_RNaseH1_WT employed for the transient overexpression of RNAseH1 in vitro on HEPA1-6 cells was a gift from Xiang-Dong Fu (Addgene plasmid #111906).
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bioRxiv - Molecular Biology 2024Quote: ... Zhang (Addgene no. 52961); pRDA_478 (Addgene no ...
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bioRxiv - Microbiology 2024Quote: Moloney MLV Env was taken from pCL-Eco (Addgene) and placed in a pCGCG backbone upstream of an IRES and GFP via homologous recombination (using New England Biolab’s HiFi Assembly kit ...
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bioRxiv - Microbiology 2024Quote: ... SIVmac239tat in pBlue and SIVmac239 LTR-Luc in pBlue were used in luciferase fusion assays along with ppMCAT1 (Addgene) to express the MCAT1 receptor.