-
No products found
because this supplier's products are not listed.
Jiahui Ding, et al.,
bioRxiv - Immunology 2021
Quote:
... ZIKA Viral RNA was isolated from Zika virus stock using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704) to create a standard curve using serial 10-fold dilutions of ZIKV RNA ...
-
No products found
because this supplier's products are not listed.
Margarita V. Rangel, et al.,
bioRxiv - Microbiology 2021
Quote:
... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
-
No products found
because this supplier's products are not listed.
Agnieszka Dabrowska, et al.,
bioRxiv - Microbiology 2020
Quote:
Zika virus RNA yields were assessed using real-time PCR on a 7500 Fast Real-Time PCR instrument (Thermo Scientific, Poland). ZIKV cDNA was amplified in a reaction mixture containing 1× TaqMan Universal PCR Master Mix (RT-PCR mix ...
-
No products found
because this supplier's products are not listed.
Sara B. York, et al.,
bioRxiv - Microbiology 2021
Quote:
... Zika capsid (GeneTex; GTX133317), Zika prM (GeneTex;133305) ...
-
No products found
because this supplier's products are not listed.
Derek D.C. Ireland, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Primary antibodies used include: human polyclonal anti-Zika virus antibody (Kerafast EVU302, MA) or mouse anti-ZikV E protein mAb (Fitzgerald ...
-
No products found
because this supplier's products are not listed.
E. Ladrón-de-Guevara, et al.,
bioRxiv - Biophysics 2019
Quote:
... The lysate was purified by Histrap FF columns (GE Healthcare) with an imidazole gradient from 0 to 0.5 M ...
-
No products found
because this supplier's products are not listed.
Wei Zhang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Adeno-associated virus (AAV) cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...
-
No products found
because this supplier's products are not listed.
Andrew V. Anzalone, et al.,
bioRxiv - Genomics 2021
Quote:
Genomic DNA from crude cell lysates was column purified (Zymo) and DNA concentrations were determined by Nanodrop (Thermo Scientific) ...
-
No products found
because this supplier's products are not listed.
Sydney B. Blattman, et al.,
bioRxiv - Systems Biology 2020
Quote:
Lysates were purified with AMPure XP beads (A63881, Beckman Coulter, Brea, CA) at a 1.8x ratio (∼99 μL) ...
-
No products found
because this supplier's products are not listed.
Hanh T. Nguyen, et al.,
bioRxiv - Microbiology 2022
Quote:
... The virus lysate was then denatured by boiling in denaturing buffer (New England BioLabs) for 10 min and treated with PNGase F or Endo Hf enzymes (New England BioLabs ...
-
No products found
because this supplier's products are not listed.
Amy Lam, et al.,
bioRxiv - Biophysics 2021
Quote:
... Sendai virus (purified Sendai Cantell Strain, batch 960216) was obtained from Charles River Laboratories (Wilmington ...
-
No products found
because this supplier's products are not listed.
Sundaram Acharya, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... The lysate was affinity purified by Ni-NTA beads (Roche) and the eluted protein was further purified by size-exclusion chromatography on HiLoad Superdex 200 16/60 column (GE Healthcare ...
-
No products found
because this supplier's products are not listed.
S.A. Codeluppi, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and pZac2.1 gfaABC1D-lck-GCaMP6f virus (Addgene virus #52924).
-
No products found
because this supplier's products are not listed.
Anna Riccio, et al.,
bioRxiv - Microbiology 2022
Quote:
... Pseudoviruses were purified using PEG Virus Precipitation Kit (Abcam), resuspended in 100 μl of virus re-suspension solution and then frozen at -80°C until use ...
-
No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
-
No products found
because this supplier's products are not listed.
Nipun Verma, et al.,
bioRxiv - Genetics 2024
Quote:
... Virus was concentrated using PEG virus precipitation (Promega # V3011). In brief ...
-
No products found
because this supplier's products are not listed.
Bryan A. Johnson, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was then inactivated and whole cell lysates taken by the addition of 2× Laemmli buffer (Bio-Rad #161073) + 2-mercaptoethanol (Bio-Rad #1610710 ...
-
No products found
because this supplier's products are not listed.
Drishya Kurup, et al.,
bioRxiv - Microbiology 2020
Quote:
A codon optimized Zika prME-NS1 gene (strain PRVABC59 Asian 2015; GenBank: ANW07476.1) was gene synthesized by GenScript. The recombinant ZIKV-NS1 was constructed as published previously (13) ...
-
No products found
because this supplier's products are not listed.
Hiroki Fukunaga, et al.,
bioRxiv - Biophysics 2023
Quote:
... and purified using the AdEasy Virus Purification Kit (Agilent Technologies). After the differentiation of C2C12 cells into myotubes ...
-
No products found
because this supplier's products are not listed.
Chelsea D. Merkel, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Virus was amplified and purified using AdenoPACK 20 Adenovirus (Ad5) purification & concentration kit (Sartorius).
-
No products found
because this supplier's products are not listed.
William J Mason, et al.,
bioRxiv - Physiology 2021
Quote:
... The virus was further purified using Sepharose G100 SF resin (Merck, Darmstadt, Germany) in Econopac colums (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Kelly Mitchell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
-
No products found
because this supplier's products are not listed.
Melissa V. Fernandez, et al.,
bioRxiv - Microbiology 2020
Quote:
... clarified cell lysates were purified by protein G affinity chromatography (Gold Biotechnology, Inc.). The b12 Fab ...
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No products found
because this supplier's products are not listed.
Tamanash Bhattacharya, et al.,
bioRxiv - Microbiology 2020
Quote:
... flies were anesthetized with CO2 and injected intrathoracically with 50nL of approximately 1010 PFU/mL of purified Sindbis virus (SINV-nLuc) or sterile saline solution (1XPBS) using a nano-injector (Drummond Scientific). Flies were collected two days post-infection ...
-
No products found
because this supplier's products are not listed.
Pia Hoellerbauer, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Lysates were sonicated (Bioruptor, Diagenode) and then quantified using Pierce BCA assay (Thermo Fisher) ...
-
No products found
because this supplier's products are not listed.
Zhihua Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ...
-
No products found
because this supplier's products are not listed.
Jonathan Burnie, et al.,
bioRxiv - Microbiology 2021
Quote:
The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ...
-
No products found
because this supplier's products are not listed.
Niraj Mishra, et al.,
bioRxiv - Microbiology 2019
Quote:
... Overnight UV-irradiation was performed to inactivate the virus in the cell lysate preparations and large debris was removed via filtering through 70 μm cell strainers (BD Biosciences).
-
No products found
because this supplier's products are not listed.
Michael B. Fessler, et al.,
bioRxiv - Immunology 2022
Quote:
... The virus sequence was confirmed by Illumina sequencing and did not differ from the parental virus ...
-
No products found
because this supplier's products are not listed.
Gloria P. Larson, et al.,
bioRxiv - Microbiology 2019
Quote:
Multicycle replication infections were performed by inoculating A549 cells at a multiplicity of infection (MOI) of 0.01 using virus diluted in virus growth media (VGM) (DMEM supplemented with penicillin/streptomycin [Corning cellgro] ...
-
No products found
because this supplier's products are not listed.
Chen-xi Zhang, et al.,
bioRxiv - Immunology 2020
Quote:
The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ...
-
No products found
because this supplier's products are not listed.
S.N. Freda, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected using a microsyringe pump (World Precision Instruments); >5 minutes elapsed before the pipette was moved from the ventral injection site to the more dorsal injection site ...
-
No products found
because this supplier's products are not listed.
Kevin J. McKernan, Liam T. Kane, Yvonne Helbert,
bioRxiv - Genomics 2020
Quote:
... 20-100% of the 1 mL lysate is purified (96 well vs Eppendorf tubes). 96 well format is eluting in 25ul and utilizing 8.6ul in qPCR ...
-
No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
-
No products found
because this supplier's products are not listed.
Eleonora Sementino, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Then virus particles and polybrene (sc-134220; Santa Cruz) at a concentration of 2 μg/ml were added to the plate ...
-
No products found
because this supplier's products are not listed.
Xin Wang, et al.,
bioRxiv - Genetics 2020
Quote:
... Lysates were incubated with control (Chromotek) or rabbit IgG beads (Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Lee A. Armstrong, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Viral proteins were affinity purified from 4mg lysates by tumbling with 40µl Strep-Tactin Sepharose (IBA Life Sciences) for 90min at 4°C ...
-
No products found
because this supplier's products are not listed.
Takanobu Tagawa, et al.,
bioRxiv - Microbiology 2022
Quote:
... The virus pellet was resuspended to EBM2 (Lonza) media ...
-
No products found
because this supplier's products are not listed.
M. Zeeshan Chaudhry, et al.,
bioRxiv - Microbiology 2021
Quote:
... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
-
No products found
because this supplier's products are not listed.
Helene Sertznig, et al.,
bioRxiv - Microbiology 2021
Quote:
... The virus containing supernatant was then purified by filtration through 0.30 μm MACS SmartStrainers (Miltenyi Biotec), aliquoted and stored at -80 °C ...
-
No products found
because this supplier's products are not listed.
Juanxian Cheng, et al.,
bioRxiv - Neuroscience 2020
Quote:
... mouse brain lysates or cell lysates (1-2 mg) with protease inhibitor cocktail were incubated with anti-MeCP2 (Cell Signaling Technology, 3456) or anti-OGT (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Hanna Jérôme, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... purified virus RNA was treated with 5 units of tobacco acid pyrophosphatase (Epicentre) to generate 5’ monophosphorylated termini ...
-
No products found
because this supplier's products are not listed.
E. Crampon, et al.,
bioRxiv - Microbiology 2022
Quote:
... Mixture reaction for pr titrations: 10 µL of purified virus (109-1010 ffu/mL) were incubated in a multi-well plate (Greiner) with increasing amounts of purified pr protein for 30 min at 37ºC in 100 mM Tris HCl pH 7.5 and 150 mM NaCl ...
-
No products found
because this supplier's products are not listed.
Irina V. Chestakova, et al.,
bioRxiv - Microbiology 2023
Quote:
... and H1N1 virus A/California/07/2009 (Sino Biological) using an arbitrary fluorescence cut-off of 6,000 ...
-
No products found
because this supplier's products are not listed.
Yang S. Chen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Lysates were sonicated by Covaris Sonicator to ~500 bp fragments (130 μL/tube ...
-
No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... virus particle containing supernatant was collected and enriched using LentiConcentrator (OriGene). Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Thomas P. Peacock, et al.,
bioRxiv - Microbiology 2020
Quote:
Purified virus binding to different sialylated receptor analogues was tested using an Octet RED bio-layer interferometer (Pall ForteBio) as described previously [21] ...
-
No products found
because this supplier's products are not listed.
Victoria L. Corbit, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ...
-
No products found
because this supplier's products are not listed.
Robbert Boudewijns, et al.,
bioRxiv - Microbiology 2020
Quote:
... virus stocks were confirmed to be free of mycoplasma (PlasmoTest, InvivoGen) and other adventitious agents by deep sequencing on a MiSeq platform (Illumina ...
-
No products found
because this supplier's products are not listed.
Erin R. Scheidemann, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... CTSD for mouse cell lysates (#MAB1123-SP) and CTSA for mouse cell lysates (AF1029-SP) were bought from R&D Systems.