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Citations for GE Life Sciences :
1 - 50 of 4253 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The lysate was purified by Histrap FF columns (GE Healthcare) with an imidazole gradient from 0 to 0.5 M ...
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bioRxiv - Biochemistry 2020Quote: ... Virus was purified by heparin affinity chromatography on an Akta FPLC (HiTrap, GE Healthcare), ultra-centrifuged overnight and resuspended in sterile Ringer’s solution ...
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bioRxiv - Developmental Biology 2022Quote: ... crude lysates were purified on a HiTrap TALON column (GE Healthcare), cleaved on the column with 3C protease followed by size exclusion chromatography (HiLoad Superdex 200 ...
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bioRxiv - Biochemistry 2021Quote: ... Crude bacterial lysates were purified by Dextrin Sepharose affinity chromatography (GE Healthcare) after overnight autoinduction and cell lysis ...
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bioRxiv - Cell Biology 2021Quote: ... Sepharose 6B purified cell lysate was incubated with IgG Sepharose (GE Healthcare) followed by buffer wash steps to remove non-specifically binding proteins ...
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bioRxiv - Pathology 2024Quote: ... Lysates were purified through binding to Fast flow Nickel sepharose (GE Healthcare) and washed with buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... crude lysates were purified on a HiTtap TALON column (GE Healthcare; Chicago, IL), cleaved on the column with 3C protease and followed by size exclusion chromatography (HiLoad Superdex 200 ...
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bioRxiv - Biochemistry 2020Quote: ... and lysates were purified by affinity chromatography via AKTA FPLC (GE Life Sciences).
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bioRxiv - Biochemistry 2019Quote: ... and the soluble lysate was purified over a glutathione affinity column (GE Healthcare). Bound complex was eluted via overnight on-column TEV cleavage to remove the GST-and His-tags ...
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bioRxiv - Immunology 2020Quote: ... lysate was affinity purified using Cobalt-charged His-Trap Chelating column (GE Healthcare) through imidazole gradient elution ...
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bioRxiv - Neuroscience 2023Quote: ... the lysate was purified using glutathione S-transferase beads (Ge healthcare, #17-5132). The proteins were eluted and concentrated with Amicon Centrifugal Filter Units 10K (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified from the lysate using Glutathione-sepharose 4B (GE Healthcare). His-tagged protein expression was induced by shifting to 15°C and adding 0.1 mM IPTG ...
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bioRxiv - Biophysics 2021Quote: ... lysate was purified on a 1 ml Strep Trap HP (GE Healthcare, Chicago, IL) using the Äkta pure purification system (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified from lysates with glutathione-Sepharose 4 Beads (GE Healthcare). His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare). The size and purity of the protein preparations were verified by SDS-PAGE and Coomassie Blue staining ...
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bioRxiv - Biophysics 2022Quote: ... Protein was affinity purified from lysate using a HisTrap HP 5 mL column (GE Healthcare) in binding buffer (50 mM NaPO4 ...
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bioRxiv - Microbiology 2020Quote: ... SepHMs-His6 was purified from lysates using an HisTrap column in ÄKTA pure (GE Healthcare) and eluted using an increasing concentration of imidazole ...
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bioRxiv - Biophysics 2019Quote: ... Soluble fractions of cell lysates were purified by a 5-ml HisTrap column (GE Healthcare) followed by a 5-ml HiTrap heparin column (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... Clarified lysates were purified by affinity purification (GSTrap or HisTrap, GE HEALTHCARE, Little Chalfont, UK) and purity was assessed by SDS-PAGE ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant myosin was purified from clarified lysate by using the AKTA purify system (GE Healthcare). First ...
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bioRxiv - Biophysics 2021Quote: ... The protein was purified from the lysate using Ni2+-NTA column of [KTA Pure (GE healthcare). For in vitro biotinylation of the polyprotein ...
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bioRxiv - Biophysics 2022Quote: ... Protein was then affinity purified from lysate using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer (100 mM tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... The proteins were purified from the cell lysate using 300 μl GST resin (GS4B, GE Healthcare), and incubated with excess purified wild-type or mutant rDPP9 proteins on ice for 60 min ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates prepared by sonication and centrifugation were purified on a HisTrap HP column (GE Healthcare). The N-terminal polyhistidine-GST tag was cleaved by incubation with TEV protease at 4 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: Lysates were purified using a 1 mL HisTrap HP Ni-sepharose column (GE Healthcare Life Sciences) connected to an ÄKTA pure protein purification system (Cytiva) ...
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bioRxiv - Biophysics 2021Quote: Purified protein or cell lysates were injected onto a Superose 6 increase 5/150 column (GE Healthcare), running in a buffer containing 20 mM Tris (pH 7.5) ...
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bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD was purified from the clarified lysate at 4°C using an ÄKTA Pure (GE Healthcare) in one day ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant of cell lysate was purified over Glutathione-Sepharose 4B (GS4B) (GE Healthcare, Boston, MA, USA) or Ni-NTA resins (Qiagen) ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant myosin was purified from clarified lysate by using the AKTA purify system as follows (GE Healthcare). First ...
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bioRxiv - Immunology 2020Quote: ... The soluble fraction was purified from the cell lysate using Glutathione Sepharose 4B beads (GS4B, GE Healthcare). The proteins were then digested with PreScission protease (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was purified from cleared cell lysate using Ni-sepharose 6 Fast flow resin (GE Healthcare) in a poly-prep column (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified from soluble fractions of the bacterial cell lysates using Glutathione Sepharose beads (GE Healthcare). Proteins were dialyzed in 1x TBS overnight at 4°C overnight and protein quantified ...
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bioRxiv - Biochemistry 2019Quote: ... Enzymes were purified from the cell lysate using a 1 ml His-Trap FF crude column (GE Healthcare) using an elution gradient from 10 to 250 mM imidazole in 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... Then the proteins were purified from the lysate with the Ni2+-NTA column of ÄKTA Pure (GE healthcare). Then the proteins were biotinylated using Avidity biotinylation kit and recommended protocol ...
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bioRxiv - Biochemistry 2019Quote: ... His6–Ub-tagged SbtB7001 was purified from cell lysate using a 5 ml HisTrap FF column (GE Healthcare): the pre-equilibrated and lysate-loaded column was washed with binding buffer (20 mM NaH2PO4 ...
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bioRxiv - Physiology 2019Quote: ... The vector was purified from the clarified lysate by affinity chromatography over a HiTrap heparin column (GE Healthcare), and ultracentrifuged overnight before resuspension in sterile physiological Ringer’s solution ...
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was first affinity-purified on an ÄKTA Purifier using His-Trap HP columns (GE Healthcare) and eluted with elution buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Physiology 2023Quote: ... The vector was purified from the clarified lysate by affinity chromatography using a HiTrap heparin column (GE Healthcare), ultracentrifuged overnight and resuspended in sterile Ringer’s solution ...
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bioRxiv - Bioengineering 2023Quote: ... The ErCas12a protein in clarified lysate was sequentially purified using immobilized metal affinity chromatography (HisTrap HP, GE Healthcare) and heparin chromatography (HiTrap Heparin HP ...
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bioRxiv - Biochemistry 2021Quote: ... and the released virus particles were purified by 55% cesium chloride and desalted through a PD10 column (GE Healthcare Life Sciences, Cat#17085101). Adenovirus particles were tail vein injected at doses of 0.1 OD for the lean ...
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bioRxiv - Neuroscience 2023Quote: ... The adeno-associated virus (AAV) vectors were produced by helper-free triple transfection and purified using affinity chromatography (GE Healthcare, Chicago, IL, USA). For production of AAV2.1 vector ...
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bioRxiv - Cancer Biology 2021Quote: ... The soluble fraction of the cell lysate was purified on HiTrap IMAC HP columns (GE Healthcare # 17-0920-05) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... Cell lysates containing Hb were initially purified using a Zn2+ charged chelating Sepharose Fast Flow resin column (GE healthcare) because Hb surfaces are rich with His residues that can bind Zn2+ (also see (42)) ...
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bioRxiv - Biophysics 2020Quote: ... The protein was purified from the supernatant of the lysate using a 5 ml HisTrap HP column (GE Healthcare). The Smt3 tag was cleaved off before further purification by a HiLoad 16/600 Superdex 75 size exclusion column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was cleared by centrifugation and SUMO-tagged IMPDH chromatographically purified with HisTrap FF columns (GE Healthcare Life Sciences) and an Äkta Start chromatography system ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... into the periplasm and purified from the periplasmic lysate via anion exchange chromatography (Q-sepharose, GE Life Sciences Cat: 25236), using 20 mM Tris-HCl pH 8.0 with 20 mM NaCl (Buffer A ...
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bioRxiv - Biophysics 2020Quote: ... coli BL21(DE3) cells and purified from the cell lysate using Glutathione-Sepharose 4B resin (Cytiva, Tokyo, Japan (GE Healthcare)) ...
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bioRxiv - Genetics 2019Quote: ... Lysate was clarified by centrifugation at 25,000xg and soluble protein was purified using Co2+ affinity chromatography (TALON column, GE Healthcare) followed by anion-exchange chromatography (Q-FF ...
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bioRxiv - Biophysics 2020Quote: ... the proteins were first purified from bacterial cell lysates using His-Trap HP (Ni-NTA) columns (GE Healthcare Life Sciences), as per the manufacturer’s instructions ...