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Citations for TianGen Biotech :
1 - 50 of 51 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ...
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bioRxiv - Microbiology 2020Quote: The DNA and RNA of virus were extracted by using TIANamp Virus DNA / RNA Kit (TIANGEN) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... And the PRV genome within 200 μL suspension was extracted by TIANamp Virus DNA/RNA Kit (TIANGEN). All cDNAs and viral genome were -20°C.
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bioRxiv - Immunology 2024Quote: RNA from both host and virus was extracted by using the RNAsimple Total RNA Kit (DP419, TIANGEN) and Virus DNA/RNA Extraction Kit (RM401-04 ...
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bioRxiv - Molecular Biology 2020Quote: ... then purified with DNA purification kit (Tiangen). RNA was removed by treatment of RNase at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... then purified with DNA purification kit (Tiangen). RNA was removed by treatment of RNase at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified by TIANgel Purification Kit (DP219, TIANGEN). See Table S2 for primer sequences ...
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bioRxiv - Cell Biology 2023Quote: ... and purified by TIANgel Purification Kit (DP219, TIANGEN). See Table S3 for primer sequences ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified using a Universal DNA Purification kit (TIANGEN) according to the manufacturer’s manuals [26-27] ...
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bioRxiv - Genetics 2019Quote: ... the PCR amplicons were purified using Gel Extraction kit (TIANGEN). A total of 200 ng of the purified PCR amplicon was denatured and slowly reannealed to facilitate heteroduplex formation ...
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ARID1a protects against steatosis and insulin resistance via PPARalpha-mediated fatty acid oxidationbioRxiv - Cell Biology 2019Quote: ... DNA was purified using a PCR Purification Kit (Tiangen,Beijing,China) and subsequently analyzed by qPCR using primers located on the Acox1 and Cpt1a promoter.
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bioRxiv - Genomics 2019Quote: ... and RNAs were purified with RNAprep Pure Micro kit (TIANGEN, DP420). The mRNA was then purified using NEBNext Poly(A ...
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bioRxiv - Cell Biology 2021Quote: ... The products were further purified with Gel Extraction kit (Tiangen, DP209) and prepared for sequencing by Illumina Novaseq 6000.
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bioRxiv - Biochemistry 2023Quote: ... DNA was purified by universal DNA purification kit (Tiangen, DP314-03) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was purified with Tiangen Microgenomic DNA extraction Kit (Tiangen, DP316), and genomic PCR analyses were performed as described in each figure ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from mouse brain lysates (mixture of 3∼4 mouse brains for each sample) using RNAprep pure Tissue Kit (Tiangen, DP431). cDNA was synthesized using HiScript II 1st Strand cDNA Synthesis Kit (Vazyme ...
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bioRxiv - Microbiology 2021Quote: ... RNA samples were then purified with the RNA clean Kit (TIANGEN, Product #DP421). Purified RNA was electrophoresed on 1% [w/v] agarose gel to monitor the integrity and contaminants.
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bioRxiv - Evolutionary Biology 2021Quote: ... Plasmids were extracted and purified using a TIANprep Mini Plasmid Kit II (Tiangen). The insertion and direction of probes were confirmed with Sanger sequencing using M13 PCR products (Tsingke) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were merged and purified by using TIANquick Midi Purification Kit (TIANGEN). The NGS libraries were prepared by using VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme ...
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bioRxiv - Microbiology 2023Quote: CT DNA was extracted from the culture supernatant or cell lysates using the TIANamp Genomic DNA kit (Tiangen Biotech, Beijing, China; DP304). Quantitative PCR analysis targeting the CT cryptic plasmid was performed using TaqMan Gene Expression Master (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and mSIN1-GFP plasmids were purified using an EndoFree Midi Plasmid Kit (TIANGEN, #DP108). A plasmid volume of 0.4 μg was applied to each coverslip in the transfection step ...
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bioRxiv - Developmental Biology 2019Quote: ... The amplified products were purified by gel electrophoresis using Universal DNA Purification Kit (TIANGEN) and cloned into a pMD19-T vector (Takara) ...
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bioRxiv - Microbiology 2023Quote: ... and the biotinylated RNA was purified using the RNAclean Kit (cat. no. 4992728, TIANGEN). In this process ...
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bioRxiv - Genomics 2019Quote: ... The PCR products were purified and cloned into the pGM-T vector (TIANGEN, Beijing, China); at least 10 positive plasmid clones were sequenced and analyzed using NCBI BLAST.
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bioRxiv - Biochemistry 2022Quote: ... One microgram of the purified total RNAs were treated with RNase-free DNase I (TIANGEN) and subsequently reverse transcribed into the first-strand cDNAs by the iScriptTM cDNA synthesis Kit (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... Then the PCR products were purified using the Agarose Gel Extraction kit (TIANGEN, Beijing, China), and transformed into Escherichia coli DH5α ...
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bioRxiv - Genomics 2021Quote: Total RNA was extracted and purified with the RNAprep Pure Plant Kit (Tiangen Biotech, Beijing, China). Poly(A)+ mRNA was enriched with oligo (dT ...
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bioRxiv - Genomics 2021Quote: Total RNA was extracted and purified with the RNAprep Pure Plant Kit (Tiangen Biotech, Beijing, China) from each sample list in Supplemental Table S2 ...
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bioRxiv - Microbiology 2020Quote: ... All the DNA samples were purified through a DNA kit column (DP214-02, Tiangen, Beijing, China) and kept at −20°C until further analysis (6) ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified and directly recombined by the EasyGeno recombinant clone kits (Cat:VI201, Tiangen). After conventional transformation and monoclonal screening of positive bacteria ...
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bioRxiv - Microbiology 2021Quote: ... Then the crude RNA was purified by RNA prep Pure Cell/Bacteria Kit (TIANGEN, Product #DP430) and genomic DNA was removed by DNase I (NEB ...
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bioRxiv - Immunology 2022Quote: ... Total RNA from collected samples was purified with the RNAprep Pure Micro Kit (TIANGEN, Beijing, China) and reversely transcribed into cDNA with a First Strand cDNA Synthesis Kit (Beyotime ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA from an intact gonad was extracted and purified using the RNAprep pure Micro Kit (TIANGEN). The quality of RNA was determined by Nanodrop ...
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bioRxiv - Bioengineering 2023Quote: ... the PCR products were purified after agarose electrophoresis (using Universal DNA purification kit, TIANGEN, Beijing, China), and their concentrations were determined by the OD 260 value by NanoDrop (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... the products of first round extension PCR were purified by gel extraction kit (TIANGEN Biotech, Beijing, China), of which 5 μL of each sample was used as the template in the second extension PCR ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were gel purified and cloned with the TIAN Quick Midi Purification Kit (Tiangen, Beijing, China) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... DNAs were extracted by hydroxybenzene-chloroform-isoamyl alcohol and purified by a Phase Lock Gel (Tiangen, China). For ChIP-seq ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were purified from agarose gels using a TIANgel Purification Kit (TianGen BioTech Co. Ltd, China), followed by nucleotide sequencing performed by Sangon Biotech (Shanghai ...
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bioRxiv - Genetics 2021Quote: ... PCR products from each sample were separately pooled and purified with a Universal DNA Purification Kit (Tiangen, China). In the second-round PCR ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted and purified following the manufacture’s protocol using the EIANamp stool DNA kit (TIANGEN, DP328-02).
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bioRxiv - Genetics 2019Quote: The single-stranded viral DNA was isolated from M13mp18 and its 3kb linear dsDNA was amplified by PCR and purified by DNA purification kit (Tiangen). A 60-nt oligomer from M13 genome ...
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bioRxiv - Neuroscience 2021Quote: ... All DNA products were separated in agarose gel to confirm sizes and following purified using TIANgel Midi Purification Kit (TIANGEN). The mixture of 0.01 pM of backbone and 0.03 pM of each insert fragments were delivered for transformation in Stable3 competent bacteria after 30 minutes incubation in 50 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... The DNA fragments were separated by agarose gel electrophoresis and purified using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Plant Biology 2022Quote: ... gDNA buffer was added into total RNA to remove DNA impurity and then the purified RNA was used as a template to synthesized cDNA using a FastKing RT Kit (TIANGEN). The RT-qPCR assays were performed on qTOWER3 Real-Time PCR thermal cyclers (Analytik Jena ...
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bioRxiv - Cancer Biology 2023Quote: ... total RNA from the cells plated on 96 well plates were first extracted and purified using the RNAprep Pure Micro Kit (TIANGEN). Reverse transcription was performed with HiScript III cDNA Synthesis Kit (Vazyme) ...
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bioRxiv - Biophysics 2022Quote: ... the PCR amplified PAM-containing products were purified by VAHTS DNA Clean Beads (Vazyme) and further amplified by TIANSeq Fast DNA Library Prep Kit (TIANGEN) for Illumina Novaseq PE150 sequencing ...
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bioRxiv - Genetics 2019Quote: ... the template DNA was linearized by XbaI digestion (for Cas9 and qCas9 mRNA) or NotI digestion (for EGFP and EGFP-PEST mRNA) and then purified by TIANquick Mini Purification Kit (TIANGEN Biotech). The capped Cas9/qCas9 and EGFP/EGFP-PEST mRNA were synthesized by mMESSAGE mMACHINE T7 or SP6 kit (Invitrogen) ...
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bioRxiv - Plant Biology 2020Quote: ... The pCSGAPO1 vector (Cong et al., 2013) was digested with Bsa I and purified using a DNA purification kit (Tiangen, China). A ligation reaction (10 μl ...
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bioRxiv - Plant Biology 2024Quote: ... the DNA fragments were separated by 1.0 % agarose gel electrophoresis (1 × TAE buffer) and purified from the gel with the DNA Gel Extraction Kit (TianGen Biotech., Beijing, China), then they were sub-cloned into pCE2 TA/Blunt-Zero vector (Vazyme ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The PCR products were purified by excision of the band from agarose gel using the Universal DNA Purification Kit (TIANGEN Bio, Beijing, China), and ligated into a pGEM®-T vector (Promega Co. ...