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Citations for Millipore Sigma :
1 - 50 of 3242 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
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bioRxiv - Microbiology 2021Quote: ... ZIKA E (Millipore Sigma, cat# MABF2046), or GAPDH (Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2020Quote: ... virus was collected after 120 hours from both cell lysates and media and purified over iodixanol (Optiprep, Sigma).
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated with mouse anti-Zika antibody 4G2 (Millipore) at 1:500 dilution for 60 min at room temperature ...
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bioRxiv - Pathology 2022Quote: ... the virus particle-containing media was harvested and virus particles were purified using Polyethylene glycol 8000 (Sigma).
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bioRxiv - Immunology 2022Quote: ... 1 μL of virus lysate was treated with 250 U of benzonase nuclease (Sigma) at 37°C for 1 h ...
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bioRxiv - Bioengineering 2021Quote: ... virus was harvested and purified using an iodixanol gradient (Optiprep, Sigma). For our 7-variant pool ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated and purified via Iodixanol gradients (“Optiprep” Sigma D1556-250mL). Centrifuge the gradients for 90 min at 46,500 rpm at 16 °C ...
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bioRxiv - Microbiology 2022Quote: ... purified high titer lysates were treated with Benzonase Nuclease (Sigma) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified from lysate using either nickel (Sigma-Aldrich) or glutathione resin (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... sucrose gradient-purified AD169r-GFP virus was transferred to a 100,000 kDa Amicon filter (Millipore), then buffer exchanged with 1× PBS ...
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Immunology 2021Quote: ... a sandwich ELISA was used in which 1% Trixon X-100-treated virus lysates were added to plates pre-coated with ConA (50 µg/ml, Sigma) and reacted with anti-Env mAbs 66 ...
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
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bioRxiv - Microbiology 2020Quote: ... virus was purified using centrifugal filter devices (Centricon Plus-70 and Biomax 100.000, Millipore Corp., Bedford, MA) and stocks with a titre between 3×109 and 3×1010 viral genome equivalents (vge ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated from media using PEG Virus Precipitation Kit (Sigma). Viral titer was determined by QuickTiter™ Lentivirus Associated HIV p24 Titer Kit (Cell Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Microbiology 2022Quote: ... and purified from bacterial lysates on glutathione agarose beads (Sigma, cat. 17-0756-01). Equal amounts of GST or GST-Trim69 proteins were then bound to 10 μg of pure porcine brain tubulin (purchased from Euromedex ...
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bioRxiv - Microbiology 2019Quote: RNA was extracted from CsCl purified reovius preparations (~1×1010 virus particles) using TRI Reagent LS (T3934, Millipore Sigma) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The virus particles were further purified by layering them over a discontinuous 5% to 50% Nycodenz gradient (Sigma-Aldrich) in TNE buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were purified from the lysate with anti-FLAG M2 affinity gel (Millipore-Sigma, USA) and eluted with 0.2 mg/ml 3xFLAG peptide in elution buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were purified from the lysate with anti-FLAG M2 affinity gel (Millipore-Sigma, USA) and eluted with 0.2 mg/ml 3xFLAG peptide in elution buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... and TSCC was purified from the cell lysate by M2 anti-FLAG affinity chromatography (Sigma) followed by size exclusion chromatography using a Superose 6 column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... varicella-zoster virus (Light Diagnostics, Varicella-Zoster Virus DFA kit, EMD Millipore, Temecula, CA), adenovirus (D3Ultra Respiratory Virus Screening & Identification kit ...
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bioRxiv - Microbiology 2020Quote: ... Viruses were titrated by infecting THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 μg/ml, Sigma) and incubating for 48 h ...
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bioRxiv - Microbiology 2019Quote: ... or PMA-treated THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 µg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants containing purified virus were applied onto HeLa cells for 48 h in the presence of 8 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Immunology 2024Quote: ... Virus was plaque-purified on Vero CCL81 cells in the presence of 25 μg/mL of cytosine arabinoside (Sigma-Aldrich), and plaques in agarose plugs were amplified on Vero CCL81 cells ...
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bioRxiv - Microbiology 2023Quote: ... the gradient-purified virus was subjected to buffer exchange using the Pur-A-Lyzer™ Maxi Dialysis Kit (20k MWCO) (Sigma) at 4°C overnight to replace the iodixanol with PBS.
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bioRxiv - Biochemistry 2022Quote: ... Flag-tagged RtcB proteins were affinity-purified from the lysates with anti-Flag M2 affinity-gel (Sigma) followed by extensive washes ...
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bioRxiv - Neuroscience 2023Quote: ... the cell lysates were clarified by centrifugation at 2,000g and then purified over iodixanol (Optiprep, Sigma; D1556) step gradients (15% ...
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bioRxiv - Cell Biology 2019Quote: ... Doxycycline-inducible OKSM virus (Millipore, SCR512) and m2rtTA virus (Cyagen Biosciences ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μL of each purified virus library were incubated with 100 μL of an endonuclease cocktail consisting of 1000U/mL Turbonuclease (Sigma T4330-50KU) with 1X DNase I reaction buffer (NEB B0303S ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was purified from the cleared lysate by affinity to Anti-FLAG M2 Affinity gel (Sigma-Aldrich) and eluted in Flag buffer containing 0.5 mg/ml 3xFLAG peptide ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified from the cleared lysate by affinity to Anti-FLAG M2 Affinity gel (Sigma-Aldrich) and eluted with pulldown buffer containing 0.1 mg/ml 3xFlag peptide ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified from the cleared lysate by affinity to Anti-FLAG M2 Affinity gel (Sigma-Aldrich) and eluted with nsp9 buffer containing 0.1 mg/ml 3xFlag peptide.
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bioRxiv - Plant Biology 2023Quote: ... GST-fusion proteins were purified by loading total bacterial lysate on a preequilibrated glutathione Sepharose column (Sigma, Germany) using fast performance liquid chromatography system (Cytiva ...
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bioRxiv - Biophysics 2023Quote: ... Lysate containing FLAG-tagged target protein was purified using ANTI-FLAG M2 Affinity Gel resin (Sigma-Aldrich, USA) and eluted with 3x FLAG peptide (APExBIO ...
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bioRxiv - Microbiology 2019Quote: ... Anti-influenza A virus NP antibodies (Millipore), Anti-TNK2 (A12 ...
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bioRxiv - Microbiology 2022Quote: ... Virus propagation was performed in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... Virus propagation was performed in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Biochemistry 2020Quote: ISW2 (FLAG-Isw2) was purified as follows: Cleared lysate was incubated with Anti-FLAG M2 affinity gel (Sigma-Aldrich) at 4 °C for 1 h and eluted with 0.1 mg/mL 3X FLAG peptide (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: Retroviral infection was conducted in 6-well plates using either 1 mL of unconcentrated virus or 200 μL of concentrated virus in 20 mM HEPES pH 7.4 and 8 μg/mL polybrene (Sigma-Aldrich). If selection was desired ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma) for 8-12 hours ...
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bioRxiv - Cell Biology 2021Quote: RNA was purified from lysates (total cellular RNA) and separately from SG or mFA core fractions using TRI-reagent (Sigma). Total RNA was approximately 10 times more concentrated than RNA from SG/mFA core fractions (∼100 ng/µl vs ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The phage lysate was further purified and concentrated using ultrafiltration (100 kDa Amicon® Ultra-15 centrifugal filter, Sigma, Australia), and the media was replaced with phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2021Quote: ... FH-nsp13 and GST-nsp13 were affinity purified from insect cell lysates using anti-FLAG M2 Affinity Gel (Sigma-Aldrich) or Glutathione Sepharose 4 Fast Flow resin (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... and then purified by mixing 17 mL PBS brain lysate with 4,5 mL Percoll Plus (Sigma, Saint Louis, Missouri, USA). After 15 min 500g centrifugation in +4 □C ...