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Citations for Thermo Fisher :
1 - 50 of 8175 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Zika virus RNA yields were assessed using real-time PCR on a 7500 Fast Real-Time PCR instrument (Thermo Scientific, Poland). ZIKV cDNA was amplified in a reaction mixture containing 1× TaqMan Universal PCR Master Mix (RT-PCR mix ...
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bioRxiv - Microbiology 2023Quote: ... Virus pellets and cell lysates were dissolved in radioimmunoprecipitation assay (RIPA) buffer (Thermo Scientific) containing protease inhibitors ...
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bioRxiv - Microbiology 2021Quote: ... cells and purified virus particles were lysed with RIPA (ThermoFisher Scientific) buffer supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Immunology 2019Quote: ... purified virus was immobilized on 96-well maxisorp microtiter plates overnight (Nunc). Wells were blocked with 0.05% PBST [0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated from virus-containing medium or cell lysates using TRIzol LS (ThermoFisher Scientific, #10296010) according to the manufacturer’s instructions and stored at −80°C for RT-qPCR ...
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bioRxiv - Microbiology 2022Quote: Purified virus particles were incubated with different concentrations of BS3 cosslinkers (ThermoFisher Scientific) for 30 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... RNA from virus stocks was purified using TRIzol Reagent (Thermo Fisher Scientific, #15596026). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was purified from the lysate using TRIzo (ThermoFisher, 15596018) and quantified on NanoDrop ...
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bioRxiv - Immunology 2022Quote: ... RNA from virus stocks was purified using TRIzol Reagent (ThermoFisher Scientific, catalog no. 15596026). Brief ...
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bioRxiv - Cell Biology 2020Quote: ... virus particles were then purified by gel filtration (7K molecular weight cut-off, Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... The cell and virus lysates were Western blotted and probed with a goat polyclonal anti-gp120 antibody (Invitrogen), the 4E10 anti-gp41 antibody (NIH HIV Reagent Program ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL of purified virus was analyzed with the Quant-it PicoGreen dsDNA Assay (Invitrogen).
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bioRxiv - Microbiology 2023Quote: Purified virus stocks were diluted in lithium dodecyl sulfate (LDS) sample buffer (Thermo Fisher Scientific) and separated by SDS-PAGE (NuPAGE Novex 10% Bis-Tris gel ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was isolated from purified virus using the GeneJET genomic DNA purification kit (Thermo Fisher), and the fidelity of the changed regions was verified by Sanger sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and quantitative PCR (RT-qPCR) was performed using 0.25 µl of lysate and Taqman Fast Virus 1-step Master Mix (Applied Biosystems) on a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysates containing AAV capsids were purified by AVB Sepharose (Thermo Fisher) in the case of AAV1 ...
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bioRxiv - Physiology 2020Quote: ... The lysate was cleared by centrifugation and purified by Ni-NTA resin (Thermofisher) and Strep-Tactin II resin (IBA ...
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bioRxiv - Molecular Biology 2023Quote: ... purified gRNA from benzonase-treated virus material was treated with DNaseI (Thermo Fisher Scientific, Waltham, MA, USA), and analyzed using an Agilent 2100 bioanalyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2019Quote: Cholesterol concentration in purified virus was determined using Amplex™ Red Cholesterol Assay Kit (Molecular Probes, Thermo Fisher) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: Cholesterol concentration in purified virus was determined using Amplex™ Red Cholesterol Assay Kit (Molecular Probes, Thermo Fisher) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: ... The purified virus was quantified using the bicinchoninic acid (BCA) protein assay kit (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The purified virus was dissolved with an equal volume of 2x Tris-Glycine SDS Sample Buffer (Thermo Fisher Scientific) and boiled for 5 min without a reducing agent ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the purified virus preparations was determined using a BCA protein assay kit (Thermo Fisher Scientific) prior to the addition of lysis buffer ...
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bioRxiv - Genetics 2019Quote: ... Total RNA was purified from the lysate using the PureLink RNA Mini Kit (Life Technologies) followed by DNAse I treatment (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The protein was purified from the clarified lysate using HisPur Ni-NTA resin (Thermo Scientific) and eluted with 250 mM imidazole ...
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bioRxiv - Immunology 2022Quote: ... The TCR-CD3 complex was purified from the lysate using Dynabeads M-280 streptavidin (Invitrogen). The beads were subsequently washed with 1X TBS and boiled with SDS-PAGE reducing buffer with β-mercaptoethanol ...
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bioRxiv - Biochemistry 2022Quote: ... Whole cell lysates or purified PDHC reactions were separated on 4-12% Bolt Gel (ThermoFisher) after heat denaturation in sample buffer (ThermoFisher) ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged RRs were purified from clarified lysate using HisPur Ni-NTA resin (Thermo Scientific) or HisPur Cobalt resin (Thermo Scientific) ...
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bioRxiv - Plant Biology 2019Quote: ... The soluble fraction was purified from the cell lysate using Glutathione Sepharose 4B beads (Invitrogen). The proteins were then digested with PreScission protease (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: Nucleic acids were extracted from purified virus samples either using the PureLink Viral RNA/DNA Mini Kit (Thermo Fisher Scientific) or by phenol/ether method combined with ethanol precipitation ...
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bioRxiv - Microbiology 2020Quote: ... The resuspended virus was purified using a 30% (wt/vol) sucrose cushion at 100,000 × g (Sorvall Discovery 90SE, Thermo Fisher) for two- and-a-half hours at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 25 μl of virus was immediately mixed with 25 μl of serially diluted (2×) protein A/G purified IgG (ThermoFisher) from mouse sera (starting at 500 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Genetics 2019Quote: ... The His-ChpBK fusion protein was purified from clarified lysates using a Ni-NTA beads (Invitrogen). Protein purity was determined by SDS-PAGE analysis and samples were quantified using the Bio-Rad protein assay and stored at −80 □.
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bioRxiv - Biochemistry 2023Quote: ... Lysates and purified proteins were separated by 4-12% Bis-Tris SDS-PAGE (ThermoFisher Scientific, US) and visualized by Quick coomassie stain (SERVA ...
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bioRxiv - Immunology 2024Quote: ... the lysate supernatant containing His-tagged proteins was affinity-purified with His-Tag magnetic beads (Invitrogen) and used for pull-down assays ...
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Cell Biology 2022Quote: ... the biotinylated proteins were purified by incubating the lysates with High Capacity Streptavidin Agarose beads (Pierce, ThermoFisher) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting lysate was purified using Ni-NTA chromatography using HisPur Ni-NTA beads (Thermo Fisher Scientific), and dialyzed using Slide-A-Lyzer dialysis cassettes (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... His-tagged FH(44-511) was purified from the lysate using HisPurTM Cobalt Spin Columns (Thermo Fisher). Columns were washed using with buffer A (50 mM sodium phosphate pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... virus with Trypan Blue (Gibco, 1µl) was injected into the lateral ventricle using glass pipette (WPI) ...
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bioRxiv - Immunology 2022Quote: ... Virus was diluted in OptiMem (ThermoFisher) and applied apically at a MOI 0.1 for two hours at 34,5°C with 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... Varicella zoster virus solution (Fisher Scientific) was dropped onto a No.1 coverslip and dried on top ...
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bioRxiv - Cell Biology 2023Quote: Cytotune OSKM Sendai virus (A16517, Invitrogen) and EmGFP reporter Sendai Virus (A16519 ...
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bioRxiv - Immunology 2021Quote: ... Total mRNA was extracted from 190 pMHC-tetramerpos purified (Supplementary Figure 1A) virus-specific T-cell populations(37) using magnetic beads (Dynabead mRNA DIRECT kit; Invitrogen, Thermo Fisher Scientific). Ten μl (~1μg ...
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bioRxiv - Cancer Biology 2023Quote: ... Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen) with an shRNA transducing vector and 2 packaging vectors ...
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bioRxiv - Immunology 2021Quote: ... Total mRNA was extracted from 190 pMHC-tetramerpos purified (Supplementary Figure 1A) virus-specific T-cell populations(37) using magnetic beads (Dynabead mRNA DIRECT kit; Invitrogen, Thermo Fisher Scientific). Ten μl (~1μg ...
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bioRxiv - Immunology 2021Quote: ... Virus was titrated by adding serial dilutions of virus supernatant (8 replicates) on VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Immunology 2024Quote: ... Infection was initiated by adding 100 µl of virus inoculum containing 100.000 PFU (MOI ∼0.5-1) in virus absorption medium (Gibco OptiPro Serum free medium + 1:100 Glutamax + 10 mM HEPES ...