-
No products found
Oliver Hsia, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Lysate was filtered through a BioPrepNylon Matrix Filter (BioDesign) then incubated with 1 mL Ni-NTA resin per litre culture for 1 hour ...
-
No products found
because this supplier's products are not listed.
Naureen Javeed, et al.,
bioRxiv - Cell Biology 2019
Quote:
... total protein lysates was isolated using NP-40 buffer (Amresco, J619) supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling Technology ...
-
No products found
because this supplier's products are not listed.
Yanhua Du, et al.,
bioRxiv - Epidemiology 2019
Quote:
The genomes of SFTS virus isolates were compiled using the SeqMan program in the LaserGene software package (DNAStar). The percentage similarities of nucleotide identity or amino acid identity were calculated using the ClustalX software[16] ...
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No products found
because this supplier's products are not listed.
Wilfred A. Jefferies, et al.,
bioRxiv - Immunology 2023
Quote:
... or LNP-B.1.617.2 (Delta) spike protein variant of the SARS-CoV-2 virus (Cat # PM-LNP-12) mRNA purchased from Promab Biotechnologies ...
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No products found
because this supplier's products are not listed.
Mireia Rovira, et al.,
bioRxiv - Immunology 2022
Quote:
... Peptides were purified using SDB-RPS columns (Affinisep). Briefly ...
-
No products found
because this supplier's products are not listed.
Eva Mittler, et al.,
bioRxiv - Microbiology 2020
Quote:
... cleared U2OS-Cas9 lysates were pre-incubated with the protease inhibitor E-64 (20 μM; Peptides International) for 20 min at room temperature (when indicated) ...
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No products found
because this supplier's products are not listed.
Kelly T. Rios, et al.,
bioRxiv - Microbiology 2024
Quote:
The gametocyte and zygote lysates were mechanically lysed with a 1 mL Dounce homogenizer (Wheaton, Cat# 357538) for one minute on ice with a tight pestle following chemical lysis ...
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No products found
because this supplier's products are not listed.
Matthew L. Turner, et al.,
bioRxiv - Microbiology 2019
Quote:
... Potassium was measured in the cleared lysates using a Jenway PFP7 flame photometer (Cole-Parmer, Stone, Staffordshire, UK).
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No products found
because this supplier's products are not listed.
Michael J. D. Daniels, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 25 μL lysate was combined with 175 μL cathepsin probe L (Z-Phe-Arg-AMC, 45 μM, Bachem) or C (H-Gly-Phe-AMC ...
-
No products found
because this supplier's products are not listed.
Trevor J. Hancock, et al.,
bioRxiv - Immunology 2022
Quote:
... as well as TGEV (transmissible gastroenteritis virus) (VMRD, Pullman, WA, USA). Normal cat serum was purchased from Jackson ImmunoResearch (West Grove ...
-
No products found
because this supplier's products are not listed.
Zhenming Jin, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and Anti-virus Drug Library (Shanghai Institute for Advanced Immunochemical Studies, SIAIS), which includes ∼10,000 compounds ...
-
No products found
because this supplier's products are not listed.
Katharina Kases, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and anti- ZC3H11A (Atlas Antibodies, heavy lysate) antibodies in Protein LoBind tubes (Eppendorf) ...
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No products found
Yuanyuan He, et al.,
bioRxiv - Microbiology 2023
Quote:
Imaging plates for virus quantification were prepared by coating coverslip-bottom wells (Cellvis) with 0.18 mg/ml BSA-biotin in PBS overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Luis E. Martinetti, et al.,
bioRxiv - Neuroscience 2021
Quote:
... we used a virus-retrobead or saline-retrobead mixture (red RetroBeads, Lumafluor, Cat# R180). When comparing different AAV serotypes ...
-
No products found
because this supplier's products are not listed.
Sheng Xiao, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected using a manual volume displacement injector (Narishige International USA, MMO-220A) connected to a glass pipette (Drummond Scientific ...
-
No products found
because this supplier's products are not listed.
Brenton T. Laing, et al.,
bioRxiv - Neuroscience 2022
Quote:
Immunostaining was conducted for axonal projections and verification of virus expression using a chicken anti-GFP polyclonal antibody (1:1000, Cat # GFP-1020, RRID:AB_10000240; Aves Labs, CA, U.S.A.) or a rabbit anti-DsRed polyclonal antibody (1:1000 ...
-
No products found
because this supplier's products are not listed.
Dora Buzas, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Control wells containing virus only (no ADAH11) as well as a positive control (a commercial monoclonal antibody (Absolute Antibody; Sb#15) recognising the S protein RBD ...
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No products found
because this supplier's products are not listed.
Melissa H. Bergeman, et al.,
bioRxiv - Microbiology 2023
Quote:
... Image segmentation and 3D rendering of HSV-1 virus particle undergoing exocytosis from Rab6a vesicle was done in Imaris (Oxford Instruments) by constructing spots and surfaces for the objects at respective image slices ...
-
No products found
because this supplier's products are not listed.
Jiansheng Huang, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... 70 nM purified human MPO (Athens Research, cat# ...
-
No products found
because this supplier's products are not listed.
Ria Lassaunière, et al.,
bioRxiv - Immunology 2023
Quote:
This study is part of national infectious disease surveillance and response to an emerging virus variant performed on surplus biological material by Statens Serum Institut, a governmental institute under the Danish Ministry of Health ...
-
No products found
because this supplier's products are not listed.
Myriam Ruault, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cleared lysate was incubated overnight with 1 μL of polyclonal antibody anti-Sir3 (Agro-bio). 50 μL of magnetic beads protein A (NEB ...
-
No products found
because this supplier's products are not listed.
Hang Gyeong Chin, et al.,
bioRxiv - Biochemistry 2019
Quote:
... >97% purified porcine tubulin (rPeptide, # T-1201-1) and >99% purified bovine tubulin (MP-Bioscience ...
-
No products found
because this supplier's products are not listed.
DaoFei Song, Lei Yin, Chang Wang, XiuYing Wen,
bioRxiv - Molecular Biology 2019
Quote:
... The protein concentration in tissue lysates was measured with a BCA protein assay kit (Boster, Wuhan, China) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Akira Kitamura, et al.,
bioRxiv - Biophysics 2023
Quote:
... FCCS measurements of the lysates were performed using an LSM510 META ConfoCor3 system (Carl Zeiss, Jena, Germany) equipped with a C-Apochromat 40×/1.2NA W Korr ...
-
No products found
because this supplier's products are not listed.
Julieta Ramirez, et al.,
bioRxiv - Genomics 2023
Quote:
Whole cell lysates were separated on a 5% SDS/PAGE containing 20 μm Phos-tag (NARD Institute), followed by western blotting with anti-CTCF antibody.
-
No products found
because this supplier's products are not listed.
Minhui Guan, et al.,
bioRxiv - Microbiology 2024
Quote:
... Each test virus was diluted to a final concentration of 100 pM with 1 × kinetic buffer containing 10 µM oseltamivir carboxylate (American Radiolabeled Chemicals, St. Louis, MO) and zanamivir (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Alisa Fox, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were washed and incubated in Accutase cell detachment solution for 10 min at room temperature to gently remove any surface-bound virus while preserving epitopes for flow cytometry analysis (Innovative Cell Technologies; as described in (39)) ...
-
No products found
because this supplier's products are not listed.
Rosalie Lipsh-Sokolik, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Xylanase activity was assayed using 100μl lysate and 0.5mM O-PNPX2 substrate (4-nitrophenyl beta-D-xylobioside, Megazyme) or cellPNP substrate (4-nitrophenyl beta-cellobioside ...
-
No products found
because this supplier's products are not listed.
Matthias Ollivier, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Biotinylated proteins were purified with Neutradivin coated magnetic beads (Spherotech) for 1 hour at room temperature ...
-
No products found
because this supplier's products are not listed.
Geng Chen, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... HcSE was purified by MBPTrap HP affinity column (Abbkine, China) and eluted with 10 mM maltose ...
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No products found
Liliana M. Sanmarco, et al.,
bioRxiv - Immunology 2023
Quote:
Pyruvate levels were quantified in BMDC lysates after 1h stimulation using EnzyChrom pyruvate assay kit (EPYR-100, BioAssay Systems), followed the procedure suggested by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Jose L. Nieto-Torres, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The in vitro kinase reaction mixtures were incubated with 350 μl clarified lysate of HeLa LC3B-KO cells (8×105 cell equivalents, prepared as described above) and glutathione-sepharose beads (Bioworld) for 3 h at 4°C on a rotator ...
-
No products found
because this supplier's products are not listed.
Surya Cayre, et al.,
bioRxiv - Developmental Biology 2020
Quote:
The following primary antibodies were used on mouse cell-derived lysates: anti-mouse milk proteins (Accurate Chemical; YNRMMSP; 1/2000), anti-mouse b-casein (a kind gift from C ...
-
No products found
because this supplier's products are not listed.
Revital Ravid, et al.,
bioRxiv - Cell Biology 2020
Quote:
For whole lysates – The nanoUPLC was coupled online through a nanoESI emitter (10 μm tip; New Objective; Woburn, MA, USA) to a Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Alex Agelidis, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Purified mouse interferon-β was purchased from PBL Assay Science (Piscataway, NJ) (12405-1).
-
No products found
because this supplier's products are not listed.
Christopher M. Jernigan, et al.,
bioRxiv - Neuroscience 2019
Quote:
Affinity-purified goat polyclonal anti-AmOA1 antibodies (21st Century Biochemicals, Inc. Marlborough, MA) were raised against a synthetic peptide acetyl-AMRNDRSPSYSMQVPQQGC-amide ...
-
The E protein composes the majority of the virion surface and it is involved with replication,...
Cat# abx061381-1MG,
1 mg USD $2001.0
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Veani Fernando, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The BH4 level in cell lysate was measured using an enzyme–linked immunosorbent assay (ELISA) Kit (Abbexa, Sugar Land, TX, Cat. No. abx354211) following manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Syed M. Moin, et al.,
bioRxiv - Immunology 2022
Quote:
... All HAssF and mAbs were purified with Galanthus nivalis lectin (GNA-gel, EY Laboratories) and protein A (rmp Protein A Sepharose ...
-
No products found
because this supplier's products are not listed.
Vi Pham, et al.,
bioRxiv - Cell Biology 2023
Quote:
... SGSH activity was measured from whole cell lysates following a two-step protocol [25] and using 4MU-αGlcNS (Chem-Impex Int’l Inc, Cat #30694) as fluorogenic substrate and with 4-methylumbelliferone (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Vera Kovaleva, et al.,
bioRxiv - Cell Biology 2020
Quote:
... PERK and ATF6 and human MANF were expressed and purified in CHO cells by Icosagen Ltd (Tartu ...
-
No products found
because this supplier's products are not listed.
Taissa Ricciardi-Jorge, et al.,
bioRxiv - Immunology 2023
Quote:
RNA was extracted and purified with HiYield TotalRNA mini kit (RBC Real Biotech Corporation, YRB50) according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Bettina Budeus, et al.,
bioRxiv - Immunology 2020
Quote:
... B cells were sort-purified and distributed at 5,000 cells in 100 μl RPMI1640 (Pan BioTech) per well ...
-
No products found
because this supplier's products are not listed.
Ming Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... The rAAV9s were purified using the ViraTrap™ Adenovirus Purification Maxiprep Kit (BW-V1260-02, Biomiga). The virus titer was determined using qPCR and adjusted to 1 × 1012 viral genomes (vg)/mL in PBS containing 4% sucrose.
-
No products found
because this supplier's products are not listed.
Margaret R. Smith, et al.,
bioRxiv - Immunology 2022
Quote:
... The purified CD4 T cells or unpurified splenocytes were labelled with 1μM of CFSE (Tonbo Biosciences, CA), by incubating in a water bath at 37°C for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Kazuya Toriumi, et al.,
bioRxiv - Neuroscience 2020
Quote:
... mRNA was purified from 2 μg of total RNA by NEXTflex poly(A) beads (Bioo Scientific, 512981), subjected to fragmentation and first and second strand syntheses ...
-
No products found
because this supplier's products are not listed.
Elisa Maria Rieckhoff, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Frog tubulin was purified from Xenopus laevis egg extract and directly labeled with NHS-ester atto565 (ATTO-TEC) according to (56).
-
No products found
because this supplier's products are not listed.
Hsu-Wen Tseng, et al.,
bioRxiv - Pathology 2021
Quote:
... at T11-13 or control sham surgery (surgical incisions were only created on skin and muscle without damaging spinal cord) followed by an intramuscular injection (i.m.) of 0.32mg/kg purified Naja pallida CDTX (Latoxan, France) or equal volume of sterile phosphate buffered saline (PBS)20 ...
-
No products found
because this supplier's products are not listed.
Michael J. Ormsby, et al.,
bioRxiv - Microbiology 2022
Quote:
Purified SlpARvB at 10 mg ml−1 was subjected to crystallization using a Mosquito liquid handling robot (TTP Labtech), with the sitting drop vapor-diffusion method ...
-
No products found
because this supplier's products are not listed.
Maciej M. Jankowski, et al.,
bioRxiv - Neuroscience 2021
Quote:
... the electrodes were cleaned by washing them with a 4% tergazyme solution in purified DDW water (Alconox, Jersey City, NJ, USA) and afterwards carefully washed in purified DDW to remove all residues of tergazyme ...
-
No products found
because this supplier's products are not listed.
Alberto Domingo López-Muñoz, et al.,
bioRxiv - Microbiology 2023
Quote:
... alone or in combination with purified recombinant proteins were placed in the lower chamber of a 96-well ChemoTx System plate (Neuro Probe # 101-5) in RPMI 1640 1 % FBS ...