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No products found
because this supplier's products are not listed.
G Cantero-Recasens, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and 20 μg/mL hyaluronidase (Sigma) after 40min of incubation at 37ºC ...
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No products found
because this supplier's products are not listed.
Susan Hromada, et al.,
bioRxiv - Systems Biology 2021
Quote:
... 20 mM Tris-HCl pH 8 (Invitrogen), 2 mM EDTA (Sigma-Aldrich) ...
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No products found
because this supplier's products are not listed.
S. Schmidt, et al.,
bioRxiv - Cell Biology 2024
Quote:
... deparaffinized microsections were rehydrated and incubated with hyaluronidase (4mg/mL in PBS, pH 5.5) followed by pronase (1mg/mL in PBS, pH 7.4, both Roche Diagnostics, Mannheim, Germany). After blocking with 5% bovine serum albumin sections were incubated with a mouse anti-human type II collagen antibody (1:1000 ...
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A partially purified, dialyzed, lyophilized powder.
Cat# LS002592,
300 ku, $230.00
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Ang Li, et al.,
bioRxiv - Cell Biology 2023
Quote:
... hyaluronidase (Worthington LS002592) and Dnase I (Worthington LS002139 ...
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No products found
because this supplier's products are not listed.
Allan Zhao, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... containing 0.3 mg/ml hyaluronidase (H3884, Merck) at RT ...
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No products found
because this supplier's products are not listed.
Andrea Walens, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and digested in collagenase and hyaluronidase (StemCell Technologies). Digestion media was removed and the cells were resuspended in red blood cell lysis buffer ...
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No products found
because this supplier's products are not listed.
Stephanie L. Rayner, et al.,
bioRxiv - Neuroscience 2020
Quote:
... sections were boiled for 20 minutes in low pH buffer (pH 6.1; Dako, CA, USA). Endogenous peroxidase activity and non-specific binding were blocked by incubation with 3% hydrogen peroxide in methanol for 15 minutes followed by 5% normal goat serum (Vector Laboratories ...
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No products found
because this supplier's products are not listed.
Hugues Richard, et al.,
bioRxiv - Molecular Biology 2024
Quote:
20 μL EB (10 mM TrisCl, pH 8.5, Qiagen) were added to the reaction ...
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No products found
because this supplier's products are not listed.
Richard Wagner, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... milk powder dissolved in TBST (20mM Tris–HCl pH 7.5, 150mM NaCl, 0.1% Tween-20) and probed with antibodies against LYN (Abcam), Tenascin C (Cell Signalling ...
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No products found
because this supplier's products are not listed.
Daniel M. Foulkes, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Tween 20 (TBS-T) in 5% (w/v) milk (pH 7.4) followed by incubation with an anti 6xhistidine antibody (Bio-Rad) overnight ...
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No products found
because this supplier's products are not listed.
Javier Rodriguez, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 20 mM Tris-HCl pH 8 using PD-10 desalting columns (GE Healthcare). 10 μM CAD was hydroxylated by incubation with 10 μM FIH ...
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No products found
because this supplier's products are not listed.
Deivys Leandro Portuondo Fuentes, et al.,
bioRxiv - Immunology 2019
Quote:
... and the pellet was resuspended in 20 mL buffer A (NaPO4 20 mM, NaCl 500 mM and imidazole 20 mM, pH 7,4) containing 5 U of DNAse (Promega) and 30 ug/mL lysozyme (Sigma ...
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No products found
because this supplier's products are not listed.
Arun Kumar Mahato, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Anti-RET C-20 antibody (Santa Cruz Biotechnology Cat# sc-1290 ...
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No products found
because this supplier's products are not listed.
Chenxi Qiu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... pH 7.5) for 20 minutes and quenched by 3M glycine (final concentration 360mM ...
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No products found
because this supplier's products are not listed.
Federica Liotti, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... using heat-induced antigen retrieval at pH 9.0 for 20 minutes, followed by primary antibody (PD-1, clone EH33; dilution 1:200) (Cell Signaling, Beverly, MA, USA) incubation for 15 minutes ...
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No products found
because this supplier's products are not listed.
Valentine Murigneux, et al.,
bioRxiv - Genomics 2020
Quote:
... 15 mM HEPES pH 8) and 20 μl of DNase I (M0303S, NEB). The Nuclease Flushing mix was loaded into the flow cell and incubated for 30 min ...
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No products found
because this supplier's products are not listed.
Jake V Aronowitz, et al.,
bioRxiv - Neuroscience 2021
Quote:
... When birds were unresponsive to a toe-pinch they were transcardially perfused with 20 ml 0.1M PBS (pH=7.4) followed by 30 ml 4% paraformaldehyde (PFA, pH=7.4; Electron Microscopy Sciences). Brains were carefully dissected ...
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No products found
because this supplier's products are not listed.
Shuting Cao, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... HEPES (pH 7.3, 20 mM; Corning, 25-060-CI) in Dulbecco’s Modified Eagle’s Medium/Ham’s F-12 50/50 Mix (Corning ...
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No products found
because this supplier's products are not listed.
Ágnes Jakab, et al.,
bioRxiv - Microbiology 2023
Quote:
... United States of America], 20 g/l mycological peptone [Oxoid, United Kingdom], 20 g/l dextrose, and 20 g/l agar [VWR International Llc, Hungary], pH 5.6). Culturing and biofilm formation were performed in RPMI-1640 (with l-glutamine and without bicarbonate ...
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No products found
because this supplier's products are not listed.
Kevin Huang, Sungjin Park,
bioRxiv - Neuroscience 2021
Quote:
... pH 7.5) before placed in primary antibody: HA (BioLegend 901501), GAPDH (Cell Signaling Technology 2118S) ...
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No products found
because this supplier's products are not listed.
Tong Gao, Eun-ah Cho, Pingfeng Zhang, Jinhua Wu,
bioRxiv - Biochemistry 2022
Quote:
... pH 7.4) containing PAC1 antibody (BD) for 1 h at 30 °C and then stained with Alexa 647 goat anti-mouse IgM (Invitrogen ...
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No products found
because this supplier's products are not listed.
Yichen Huang, et al.,
bioRxiv - Biophysics 2021
Quote:
A total of 4.0 μl of 20 μM TpCorC protein in 20 mM HEPES (pH 7.5) containing 0.03% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, USA) and either 150 mM NaCl or 150 mM KCl was mixed with 0.5 μl of 2 mM EDTA ...
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No products found
because this supplier's products are not listed.
Carly Strelez, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and 20 μg/mL hyaluronidase (MP Biomedicals, #0210074080) for 30 minutes at 37°C ...
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No products found
because this supplier's products are not listed.
Rasa Elmentaite, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and 600 IU of Hyaluronidase (Calbiochem) on a shaking platform (750 rpm ...
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No products found
because this supplier's products are not listed.
Katrina M Longhini, Michael Glotzer,
bioRxiv - Cell Biology 2022
Quote:
... 20% 250mM HEPES pH 7.5] on a Poly-D-Lysine (R&D Systems)-coated coverslip mounted on a slide with double-sided tape (3M ...
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No products found
because this supplier's products are not listed.
Victor M. Bii, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and antigenic sites were unmasked using either Tris-EDTA (10mM Tris-HCl, pH 9.0; 1mM EDTA; 0.05% Tween-20) or citric acid-based unmasking solution (Vector Laboratories) in Pascal pressure chamber (Dako) ...
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No products found
because this supplier's products are not listed.
Hong-Gyun Lee, et al.,
bioRxiv - Immunology 2024
Quote:
... containing 20 mM Tris-HCl (pH 7.5) (Teknova, #T5075), 1000 mM LiCl (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Filippo Spriano, et al.,
bioRxiv - Cancer Biology 2024
Quote:
Internalization was assessed using the Incucyte Fabfluor-pH Antibody labeling reagent (Sartorius, Germany), following the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Eli J. Cohen, et al.,
bioRxiv - Microbiology 2019
Quote:
... equipped with 20× objective lens (UCPLFLN 20×PH, 0.7 N.A.; Olympus), a high-speed camera system (LRH1540 ...
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No products found
because this supplier's products are not listed.
Satarupa Bhaduri, et al.,
bioRxiv - Cell Biology 2022
Quote:
... pH 7.5) and 20 µL of GFP Trap (Chromotek) were added ...
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No products found
because this supplier's products are not listed.
Hiraku Takada, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 20 mM Tris:HCl pH 7.5) in a zonal rotor (Ti 15, Beckman, 17 hours at 21,000 rpm). The peak containing pure 70S ribosomes was pelleted by centrifugation (20 hours at 35,000 rpm) ...
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No products found
because this supplier's products are not listed.
San-Pin Wu, et al.,
bioRxiv - Cell Biology 2024
Quote:
... in TBST (20 mM Tris, pH 7.4 (Lonza, Morrisville, NC, USA), 140 mM NaCl (Lonza) ...
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No products found
because this supplier's products are not listed.
Alex Plata, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Then membranes were rinsed in Tris-Buffer (pH =7.4) with 0.1 % of Tween-20 and incubated with HRP-conjugated secondary antibodies – anti-mouse IgG (Jackson Immunoresearch, 715-005-150, Ely, UK) or with anti-rabbit IGG (Abcam ...
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No products found
because this supplier's products are not listed.
Ariane J. Brown, et al.,
bioRxiv - Microbiology 2023
Quote:
... antigen retrieval (20 minutes at 100°C in Leica Bond-Epitope Retrieval Solution 2 pH-9) was performed on dewaxed tissues prior to labeling with CD4 antibody (ab183685 ...
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No products found
because this supplier's products are not listed.
Abdel Nasser Hosein, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... All antibodies were diluted in InVivoPure™ pH 7.0 Dilution Buffer (Bio X Cell) and administered to mice ...
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No products found
because this supplier's products are not listed.
Suvrajit Saha, Jason P. Town, Orion D. Weiner,
bioRxiv - Cell Biology 2022
Quote:
... Membranes were blocked for at least 1 hr in a 1:1 solution of TBS (20 mM Tris, 500 mM NaCl, pH 7.4) and Odyssey Blocking Buffer (LI-COR) followed by overnight incubation at 4 °C with primary antibodies diluted 1:1000 in a solution of 1:1 TBST (TBS + 0.2% w/v Tween 20 ...
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No products found
because this supplier's products are not listed.
C. Peerboom, et al.,
bioRxiv - Neuroscience 2023
Quote:
... pH adjusted to 7.2 with KOH) and ACSF was supplemented with 20 μM DNQX (Tocris) and 50 μM DL-APV (Tocris) ...
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No products found
because this supplier's products are not listed.
Jenna Kerry, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 20 mM Sodium Lactate pH 7.4 (Fisher Chemicals) or 25 mM Sodium 57 Oxamate (Alfa Aesar).
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No products found
because this supplier's products are not listed.
Roberto Efraín Díaz, et al.,
bioRxiv - Biochemistry 2024
Quote:
... 0.1 M Sodium Acetate (pH 3.6, Hampton Research HR293301; pH 4.1, Hampton Research HR293306; pH 5.0, Hampton Research HR293315; pH 5.6, Hampton Research HR293321), and 0.2 M Magnesium Chloride (II ...
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No products found
because this supplier's products are not listed.
Xinming Wang, et al.,
bioRxiv - Bioengineering 2021
Quote:
... and 1:20 000 diluted anti-GAPDH antibodies (Proteintech). Membranes were then incubated with 1:10,000 diluted HRP-conjugated secondary antibodies (Cell Signaling ...
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No products found
because this supplier's products are not listed.
René Rauschmeier, et al.,
bioRxiv - Immunology 2021
Quote:
... antibody alone or in combination with IL-4 (20 ng/ml, Peprotech) for up to 1 day ...
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No products found
because this supplier's products are not listed.
Xiaowei Gu, Anna Heinrich, Tony DeFalco,
bioRxiv - Developmental Biology 2022
Quote:
... The remaining cells were pelleted and resuspended in 80 µl of MACS buffer (PBS pH 7.2, 0.5% BSA and 2 mM EDTA) and 20 µl anti-CD45 MicroBeads (Miltenyi Biotec #130-052-301) or 20 µl anti-F4/80 MicroBeads (Miltenyi Biotec #130-110-443 ...
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No products found
because this supplier's products are not listed.
Paul R. Dominguez Gutierrez, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Hyaluronidase 2 polyclonal antibody conjugated with PE or Alexa-488 was obtained from Bioss Antibodies ...
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No products found
because this supplier's products are not listed.
Disha Sood, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... and hyaluronidase SD (Seikagaku 2.5mU/ml). Following ethanol precipitation and proteinase K heat inactivation ...
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No products found
because this supplier's products are not listed.
E. Angelo Morales, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and 20 μg/ml phleomycin (InvivoGen; ant-ph-1). For CACO-2BBE cells ...
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No products found
because this supplier's products are not listed.
Satoe Takahashi, et al.,
bioRxiv - Pathology 2024
Quote:
... 20 HEPES (pH 7.5) in Synergy Neo2 (Agilent/BioTek). The fluorescence intensities of mVenusp.H148Q/p.I152L and mTq2 were simultaneously measured in a time-dependent manner using Synergy Neo2 (Agilent/BioTek ...
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No products found
because this supplier's products are not listed.
Darshika Tomer, et al.,
bioRxiv - Cell Biology 2022
Quote:
... pH 7.4 containing 0.05% Tween 20 and 10% blocking buffer (Rockland, cat # MB-070). Arrays were then washed with PBS ...
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No products found
because this supplier's products are not listed.
Daniel L. Matera, et al.,
bioRxiv - Bioengineering 2020
Quote:
DexVS gels were formed via a thiol-ene click reaction at 3.3% w/v (pH 7.4, 37°C, 45 min) with VPMS crosslinker (12.5, 20, 27.5 mM) (GCRDVPMSMRGGDRCG, Genscript, George Town, KY) in the presence of varying amounts of argininylglycylaspartic acid (RGD ...
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No products found
because this supplier's products are not listed.
Zhiyong Liu, et al.,
bioRxiv - Cell Biology 2020
Quote:
... GFP-AKT-PH (plasmid# 21218) and GFP-PLC δ –PH (plasmid # 51407) plasmids are acquired from Addgene. pCDNA3.1-Flag-LRRK2R1441C is generated as previously described41 ...
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No products found
because this supplier's products are not listed.
Alexandra P. Frazier, et al.,
bioRxiv - Neuroscience 2023
Quote:
... were washed in PBS and antigen retrieval was performed with a 10mM tris-EDTA buffer with 0.05% Tween 20 (pH 9) for 10 min at 550 W in a PELCO BioWave Pro tissue processor (Ted Pella, Redding, CA). Sections were then blocked and permeabilized with 5% normal donkey serum (NDS ...