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Citations for Biotek :
1 - 50 of 97 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... 20 HEPES (pH 7.5) in Synergy Neo2 (Agilent/BioTek). The fluorescence intensities of mVenusp.H148Q/p.I152L and mTq2 were simultaneously measured in a time-dependent manner using Synergy Neo2 (Agilent/BioTek ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were washed 5x with 350 µL of assay buffer (50 mM Tris pH 7.4, 200 mM NaCl, 0.01% Tween 20) on a 50TS microplate washer (BioTek, United States). Plates were blocked for 1 hour in 200 µL assay buffer with 0.5% BSA (Rockland ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed three times with 300 μL per well of PBST (PBS 1X, pH 7.4, 0.05% Tween-20) using a plate washer (BioTek 405 TS). 200 μL of blocking buffer (PBST wash buffer ...
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bioRxiv - Microbiology 2022Quote: ... Unbound material was removed by washing 3 times with 300 μL/well of phosphate buffered saline + 0.05% Tween 20 (PBS-T, pH 7.5) using a BioTek 405 TS microplate washer (BioTek Instruments Inc., Winooski, VT, USA).
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bioRxiv - Microbiology 2022Quote: ... AM readings were performed by measuring fluorescence at 340/11 nm and 380/20 nm excitation and 508/20 emission every 20 seconds for 5 minutes using a Cytation 5 (BioTek). The solution was replaced with Ca2+-free Ringer’s solution containing either 2 μM thapsigargin (TG ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.4 (PBS-T) using a Microplate 405 washer (BioTek). 100 μl plasma samples diluted 1:10 in plasma diluent (PBS/5% skim milk/0.1% Tween-20 ...
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bioRxiv - Cell Biology 2021Quote: ... and fluorescence (485/20 excitation and 528/20 emission) and luminescence were measured with Cytation5 (BioTek Instruments).
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bioRxiv - Bioengineering 2024Quote: ... Approximately 20 ug of protein from each sample was resolved on a 4-20% gradient gel (Biotek), then visualized by SimplyBlue SafeStain.
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bioRxiv - Cell Biology 2019Quote: ... pH 7.0) using an automated BioTek 405 TS microplate washer (BioTek Instruments, Winooski, VT). A post-washed volume of 100 μL was dispensed and the plate was transferred into the BioTek Cytation V plate reader and each well was dispensed with 100 μL HEPES buffer containing MI-FZ3 dye to a final concentration of 1 μM ...
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bioRxiv - Cell Biology 2022Quote: ... The fluorescence intensity was measured by a filter-based Synergy Neo 2 Multi-Mode microplate reader using 420 BP 20 nm or 485 BP 20 nm excitation and 532 BP 40 nm emission band-pass filters (BioTek). All solutions were prepared in HEPES buffer (pH 7.4) ...
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bioRxiv - Molecular Biology 2023Quote: The fluorescence was measured with excitation at ~485/20 nm and fluorescence emission at ~528/20 nm using a microplate reader (Synergy HT, BioTek). The PLA2 activity was determined based on a standard curve ranging from 5 U/ml to 0.2 U/ml.
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bioRxiv - Biophysics 2024Quote: ... Saturated cultures were then diluted 1:50 in LB-kan/spec media and grown in the presence and absence of 1 μM aTC for 6 hours before OD600 and GFP fluorescence (Gain: 40; Excitation: 488/20; Emission: 525/20) were read on a multiplate reader (Synergy HTX, BioTek). Fluorescence was normalized to OD600 ...
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bioRxiv - Microbiology 2023Quote: ... and the fluorescence (Ex: 485/20, Em: 528/20) and absorbance (OD600) were measured in a Synergy Hybrid HTX Microplate Reader (BioTek Instruments). One quarter of the unstained wells were processed for cell viability and one quarter were processed for crystal violet staining to assess total biofilm formation ...
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bioRxiv - Cell Biology 2021Quote: ... Dissolved SRB (10 mM Tris-base, pH 10) was quantified (564 nm, Synergy HT BioTek reader). CDDP treatments were done in quadruplicate in at least three independent experiments ...
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bioRxiv - Biochemistry 2021Quote: ... pH set to 7.4 with NaOH) and the 96-well plate was incubated inside Cytation3 (BioTek) for 40 min at 37°C and 5 % CO2 ...
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bioRxiv - Microbiology 2023Quote: ... The fluorescence intensity of FM4-64 was measured at 515/20 nm excitation and 635/20 nm emission wavelength using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, United States). A non-coated plate was used with EVs as a control.
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bioRxiv - Cell Biology 2022Quote: ... pH 2.5 and proteoglycans levels were measured by imaging the blue colonies using automated microscope (BioTek Lionheart LX).
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bioRxiv - Microbiology 2023Quote: ... the reporting planktonic expression of gadB was tested on six pH values from 4.5 to 7.0 using a microplate reader (Synergy H1, Biotek) (Supplementary material Figure S2).
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bioRxiv - Immunology 2021Quote: ... The next day plates were washed 4 × in Tris Buffered Saline Tween (TBST-20mM Tris pH 8, 150mM NaCl, 0.1% Tween) using a plate washer (BioTek) and blocked with Casein (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were washed 3 times with 1% acetic acid and dye was solubilized in 10mM Tris pH 10.5 before measuring absorbance at 510nm on an Epoch plate reader (BioTek). Relative growth was determined as (treatment absorbance / control absorbance ...
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bioRxiv - Molecular Biology 2022Quote: ... production catalyzed by SARM1 was measured after adding the reaction reagents containing 50 μM PC6 and 100 μM NAD in 50 mM Tris-HCl (pH 7.5) on a Synergy H1 Hybird Reader (BioTek). The initial rate (RFU/min ...
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bioRxiv - Immunology 2022Quote: ... The next day plates were washed 4 × in Tris Buffered Saline Tween (TBST-20mM Tris pH 8, 150mM NaCl, 0.1% Tween) using a plate washer (BioTek) and blocked with Casein (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... The plate was then cultured for 1 day before washing with assay buffer (1x HBSS, 10 mM HEPES, pH 7.4) in a ELx405 CW plate washer (BioTek Instruments). The cells were then incubated with 1.25 µM calcium indicator Fluo-8 AM (AAT Bioquest ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... TMT-labeled peptides were then separated by high pH reverse-phase high performance liquid chromatography (HPLC) with C18 columns (Agilent BioTek) and dried in a vacuum centrifuge ...
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bioRxiv - Microbiology 2024Quote: ... for 20 min and were imaged with a Cytation 3 Imager (BioTek). The diameter of SAG1-positive vacuoles was determined using CellProfiler 4.2.4(60) ...
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bioRxiv - Genetics 2022Quote: ... The OD600 was recorded every 20 min in an Epoch2 microplate reader (BioTek) to determine doubling time ...
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bioRxiv - Microbiology 2024Quote: ... The fluorescence was measured every 20 min using a platereader (Synergy HTX, BioTek) for 24 h under the optimal excitation and emission wavelengths for each fluorescence protein (Ex = 575 nm and Em = 620 nm for mCherry ...
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bioRxiv - Microbiology 2020Quote: ... pH 7 by reading absorbance at 486 nm every minute for 15 minutes at room temperature in microtiter plates (Biotek Synergy H1M). β-lactamase activity was calculated as initial reaction rate (Vmax ...
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bioRxiv - Cancer Biology 2021Quote: ... Fluorescence images were acquired using a Lionheart FX Automated Microscope with magnification × 20 (BioTek).
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bioRxiv - Synthetic Biology 2019Quote: ... for a total of 20 readings using Gen5 data analysis software version 3.08 (BioTek). This experiment was performed twice ...
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bioRxiv - Bioengineering 2023Quote: ... and allowed to equilibrate for another 20 min before measuring bioluminescence signal (Cytation5, BioTek).
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bioRxiv - Microbiology 2024Quote: ... and A600 measurements were taken every 20 min using a platereader (Synergy HTX, BioTek). The growth rates of the exponential phase were calculated.
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bioRxiv - Microbiology 2024Quote: ... and the fluorescence intensity was monitored every 20 min in a platereader (Synergy H1, BioTek) for 18 h using an excitation wavelength of 493 nm and an emission wavelength of 516 nm ...
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bioRxiv - Biophysics 2024Quote: ... Emission: 525/20) of each well was read using a multiplate reader (Synergy HTX, BioTek). Fluorescence was normalized to OD600 for each well ...
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bioRxiv - Cell Biology 2024Quote: ... 100 µL of Tris (pH 10) was added and optical density was measured at 540 nm in Biotek Cytation 3 reader (Biotek Instruments, Winooski, VT, USA) and analyzed using the Wave Desktop 2.6.1 software (RRID:SCR_014526).
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bioRxiv - Microbiology 2021Quote: ... Cells were grown at 30°C for 20 h in a microplate reader (Synergy 2, BioTek), and the optical density at 600 nm (OD600 ...
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bioRxiv - Neuroscience 2022Quote: ... 480 Em for 20 hours at 15 min intervals at 37°C using Synergy H1 (Biotek) microplate reader ...
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bioRxiv - Physiology 2023Quote: ... The change in absorbance was monitored continuously over 20 minutes using ELISA plate reader (Biotek, USA) at a wavelength of 340 nm.
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bioRxiv - Neuroscience 2023Quote: ... 480 Em for 20 hours at 15 min intervals at 37°C using Synergy H1 (Biotek) microplate reader ...
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bioRxiv - Synthetic Biology 2024Quote: ... Growth was measured every 20 min for 15 hours in a plate reader (Synergy H1, BioTek) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... with 5 s shaking every 20 min in a 96-well plate reader (Synergy; BioTek, Winooski, VT) at 600 nm ...
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bioRxiv - Neuroscience 2021Quote: ... each well was washed with TBST buffer (0.05% Tween 20 in TBS) using ELx406 plate washer (BioTek). Diluted samples (Brain lysates = 1:5 ...
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bioRxiv - Microbiology 2024Quote: ... Optical density measurements (OD600) were performed every 5 minutes over 20 hours on a Synergy H1 (Biotek) microplate reader (chamber supplemented with 5% CO2 ...
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bioRxiv - Physiology 2019Quote: ... luminescence was measured for 20 seconds using a Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA). Calculations ...
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bioRxiv - Systems Biology 2021Quote: After measuring the OD at 600 nm at 20 minutes intervals in an automatic plate reader (ELx808, Biotek), the growth rates for the plate experiments (3 replicates per plate for 3 independent plates ...
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bioRxiv - Immunology 2021Quote: ... all the plates were washed with PBS+0.01% Tween 20 (PBST) with the ELISA washer (BioTek, Sursee, Switzerland). The rest of the procedure is identical as described above ...
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bioRxiv - Microbiology 2024Quote: ... Cultures were incubated at 37 °C with agitation for 20 hours in a Synergy H1 plate reader (Biotek) with OD600 measurements at 10-minute intervals.
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bioRxiv - Cancer Biology 2021Quote: ... Dimerization of Homovanilic acid was recorded every 30 seconds for 20 minutes with a BioTek Synergy H1 platereader (BioTek) spectrophotometer with an Ex = 320 nm and Em = 420 nm.
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bioRxiv - Synthetic Biology 2021Quote: ... em 528/20) was collected every 5 minutes with a Synergy 2 multi-mode microplate reader (BioTek, Winooski, VT) for 6 hours at 37 °C.
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bioRxiv - Biochemistry 2022Quote: ... The coated plates were blocked with 2%MPBST and washed with MilliQ + 0.05% Tween 20 in an ELISA washer (BioTek). Crude supernatant containing scFv diluted 1:1 with 2%MPBST was transferred to the corresponding well of both the antigen-coated and the control plates ...