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Citations for Cell Signaling Technology :
1 - 50 of 7755 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... using heat-induced antigen retrieval at pH 9.0 for 20 minutes, followed by primary antibody (PD-1, clone EH33; dilution 1:200) (Cell Signaling, Beverly, MA, USA) incubation for 15 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysis buffer (#9803, Cell Signaling Technology, composition: 20 mM Tris-HCl (pH 7.5), 150 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... 20 µL CDCP1 antibody (D1W9N, Cell Signaling) was added into 1 mL cell lysate and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibody dilutions: 1:20 000 GAPDH (Cell Signaling, 2118), 1:5 000 SLX4IP (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... The membranes were blocked with 5% non-fat skin milk dissolved in Tris-buffered saline-0.1% tween 20 pH 7.4 (1h30, room temperature) and incubated with rabbit polyclonal anti-phospho-S6S235/236 (1/1000; Cell Signaling) or rabbit monoclonal anti-Raptor (1/500 ...
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bioRxiv - Biophysics 2023Quote: ... The membrane was washed 3 times for 5 min in TBST (20 mM Tris pH 7.6, 150 mM NaCl, 0.1% Tween20) before incubation with anti-mouse-HRP (Cell Signaling Technologies) at a 1:2000 dilution in blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were rabbit polyclonal antibody against heme-oxygenase-1 (Cell Signaling Tech., 1:20 dilution) and mouse monoclonal antibody against iNOS (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 20 μL of a solution containing secondary antibody (Cell Signaling Technologies, cat # 4414S ...
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bioRxiv - Cancer Biology 2022Quote: ... harvested at 50-80% confluence and lysed by sonication in RIPA buffer (Cell Signaling Technology; 20 mM Tris-HCl (pH 7.5), 150 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-Keratin 20 antibody (D9Z1Z) (ab13063, Cell Signaling Technology, 1:500). Slides were then washed in TBST and incubated with Dako envision anti-rabbit or anti-mouse labelled polymer-HRP (Dako ...
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bioRxiv - Cell Biology 2022Quote: ... pH 8.8 (20 mM Tris-Cl, 2 mM EDTA, 2% w/v sodium deoxycholate, 1X protease inhibitor cocktail (Cell Signaling Technology, 5871). Extracts were carefully transferred to Eppendorf tubes containing 1 μl (250 units ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 7.4) supplemented with 5 % BSA and HRP-conjugated rabbit anti-mouse antibody (Cell Signaling Technologies, USA) diluted 1:2000 as a secondary antibody.
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bioRxiv - Biochemistry 2023Quote: ... 20 μl of peroxidase-conjugated secondary antibody (1:3000, #7076, Cell Signaling Technology,) was added into each well and incubated for 0.5-1h ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.4) (3x5 min) in a moist chamber containing the Myc-Tag (9B11) mouse antibody (Cell Signaling Technology) diluted 1:50 with incubation buffer ...
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bioRxiv - Genetics 2022Quote: ... The following antibodies were used: IgG isotype (Cell Signaling, #66362, negative control, 1:20), H3K4me3 (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.6) for 1 h and then incubated with primary antibodies (anti-phospho-AKT-Ser473 (#4060, Cell Signaling Technology, USA), anti-AKT (#9272 ...
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bioRxiv - Cancer Biology 2022Quote: Human cancer tissue array staining was subjected to antigen retrieval at 98 °C for 20 min in EDTA buffer (pH 9.0) in a steamer and incubated with a rabbit anti-cleaved caspase 3 antibody (Cell Signaling, 9664L) 1:150 overnight at 4 °C followed by anti-rabbit 594 (Invitrogen A11012 ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were then suspended in 50 µl Na-Phosphate buffer and incubated at room temperature for 10 minutes with desired antibody [20 µl of CTCF antibody (Cell Signaling Technology #2899) or 11 µg of anti-rabbit IgG (Thermo)] ...
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bioRxiv - Developmental Biology 2023Quote: ... pH 7.6 (Rabbit anti-HA, Cell Signaling, 37245 ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were fixed with 4 % paraformaldehyde and 0.2 % picric acid in phosphate buffer (pH 6.9) for 30 minutes at room temperature and incubated with peroxidase-conjugated anti-rabbit antibody (Cell Signaling technology #7074) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were incubated in AlexaFluor 647 conjugated Ki67 antibody (1:20, rabbit mAb, clone D3B5, Cell Signaling). Flow cytometry data was acquired by BD-LSRII flow cytometer.
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bioRxiv - Immunology 2022Quote: ... Chromatins were then immunoprecipitated with 20 μg of anti-p21 Waf1/Cip1 (12D1) antibodies (Cell signaling, #2947) or with equal amount of rabbit IgG control isotype ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1% Tween-20) for 1h and incubated with primary antibodies against IKKα (2682, Cell signaling, 1:1000), IKKβ (10AG2 ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein A was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 15ul Rabbit Monoclonal anti-UPF1 antibody (Cell Signaling Technology, Cat# 12040) or 5ug Normal Rabbit IgG Polyclonal Antibody (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein A were washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 15ul Rabbit Monoclonal anti-UPF1 antibody (Cell Signaling TECHNOLOGY, Cat# 12040) or 5ug Rabbit polyclonal anti-phospho-Upf1 (Ser1127 ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% Tween-20 in 1x TBS) and then treated with primary antibodies (YAP/TAZ, anti-rabbit, Cell Signaling, 8418 and beta-Actin ...
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bioRxiv - Physiology 2022Quote: ... 0.1 % Tween-20) for 1 h and incubated overnight with phospho-S6 ribosomal protein antibody (#2211, Cell Signaling) in 1 % BSA TBST at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-p62 ph-S403 (1:1000, Cell Signaling); rabbit anti-ATG14 ph-S29 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-p62 ph-S349 (1:1000, Cell Signaling); rabbit anti-p62 ph-S403 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-ATG14 ph-S29 (1:1000, Cell Signaling); rabbit anti-ATG9A (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.5) containing protease and phosphatase inhibitors (Cell Signaling). Proteins in cell lysates were resolved by SDS-PAGE and transferred to polyvinylidenedifluoride (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.5) containing protease and phosphatase inhibitors (Cell Signaling). Proteins were resolved on SDS-polyacrylamide gels (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... CD16 (Cell Signaling D1N9L; 1:500, TRS-low pH), CD56 (Agilent 123C3 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with 20 μl of rabbit anti-p44/42 primary antibody (Cell Signaling Technology, Danvers, MA, 9101), prepared in blocking buffer at 1:20 dilution for 12 h at 24°C in a humid chamber with gentle shaking ...
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bioRxiv - Genomics 2022Quote: ... 0.1% Tween-20) for one hour and incubated with primary antibodies against GR (12041, Cell Signaling Technology, 1:1000), PAXIP1 (ABE1877 ...
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bioRxiv - Microbiology 2021Quote: ... Blots were blocked in 0.05% tween-20/5% Non-fat milk in Tris-buffered saline and probed with the indicated antibodies from Cell Signaling: GAPDH (#3683) ...
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bioRxiv - Physiology 2019Quote: ... Sections were then placed in 0.03% SDS for 10 min and placed in 4% normal serum + 0.4% Triton X-100 + 1% BSA (fraction V) for 20 min before incubation for 48h with a rabbit anti-pSTAT3 antibody (1:1,000, Cell Signaling). The primary antibody was localized with Alexa Fluor 568 Goat anti-Rabbit IgGs (Invitrogen ...
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bioRxiv - Plant Biology 2019Quote: ... Detection of phosphorylated proteins was performed in blocking solution (5% BSA, 0.1% [v/v] Tween-20) with anti-phosphothreonine antibodies (9381, Cell Signaling) and with anti-rabbit-HRP (W4011 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and incubated in 5% non-fat dry milk in 1× PBS and 0.1% Tween 20 with the primary antibodies (PHC1 Cell Signaling #13505 1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... and blots (20-50 µg of total protein) were probed with the following antibodies: HA (3724S, Cell Signaling; 1:1500), BRCA1 (SC6954 ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked in 5% nonfat milk in Tris-buffered saline supplemented with 0.5% Tween-20 and probed with the following primary antibodies: anti-Stat1 (1:1000, Cell Signaling), anti-Trem2 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatants were collected and analyzed on 4-20% SDS-PAGE with anti-GFP antibodies (Cell Signaling Technologies D5.1, #2956). Anti-α-tubulin antibodies (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.5) containing protease inhibitors (Cell Signaling Technology, Danvers, Massachusetts). Proteins were resolved on SDS-polyacrylamide gels and transferred to polyvinylidenedifluoride (PVDF ...
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bioRxiv - Immunology 2023Quote: ... and CD14 (Cell Signaling D7A22T; 1:250, TRS-low pH) on a Dako Autostainer Link ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times with TBS-T (0.05% v/v Tween-20 in TBS) before incubation with HRP conjugated antibody (Cell Signaling Technology 7076S ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were washed with tris-buffered saline with Tween 20 (TBST) three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 0.05% Tween-20) and treated with the corresponding primary antibody (anti-β-actin and anti-APP; Cell Signaling Technology, Boston, USA) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... skin milk powder in PBST with 0.1% Tween 20) for 1 hour at RT and probed overnight with primary antibody (anti-HA, mAb #2367, Cell Signaling Technology) at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the membranes were blocked with 5% bovine serum albumin in phosphate-buffered saline-0.1% Tween 20 and probed with rabbit anti-PPARγ antibody (#2443S, Cell Signaling Technology) and phospho-ser273 PPARγ (bs-4888R ...
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bioRxiv - Synthetic Biology 2021Quote: ... and incubated in 5% non-fat dry milk in 1× PBS and 0.1% Tween 20 with the primary antibodies (PHC1 Cell Signaling #13505 1:1000; Rybp Cell Signaling D8J7W 1:1000 ...