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Citations for Seikagaku :
1 - 20 of 20 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... and hyaluronidase SD (Seikagaku 2.5mU/ml). Following ethanol precipitation and proteinase K heat inactivation ...
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bioRxiv - Biochemistry 2021Quote: ... before resuspending in 6.7 ml per g wet weight in MP2 (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase from Seikagaku Biobusiness) and incubated at 30°C for 60 min ...
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bioRxiv - Biochemistry 2022Quote: ... yeast cells were resuspended in 6.7 ml per g wet weight MP2 buffer (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase from Seikagaku Biobusiness) and incubated for 1 h at 30°C ...
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bioRxiv - Cell Biology 2022Quote: ... were suspended in 20 mL ice-cold 50 mM Tris–HCl (pH 7.4) buffer containing 1 mg/mL lysozyme (Seikagaku Corporation, 100940) and 1× protease inhibitor cocktail (Nacalai Tesque ...
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bioRxiv - Cell Biology 2019Quote: ... HA in the samples was digested down to disaccharides using hyaluronidase SD (2.5 milliunits/μl; 100741-1A, Seikagaku America, Inc.) and labeled with 2-aminoacridone (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... 10 mM EDTA (pH 8.0) containing 0.125 mg of Zymolyase 20T (Seikagaku) at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 20 µL of β-mercaptoethanol and 20 mg of Zymolyase 100T (Seikagaku Kogyo Co., Tokyo, Japan) were added and incubated at room temperature for 15 min to degrade the cell wall ...
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bioRxiv - Synthetic Biology 2020Quote: ... we added 20 µl of a 2 mg/ml Zymolyase stock solution (SEIKAGAKU, 20 U/mg) to the cell suspension and incubated it for 1h at 37°C for cell wall digestion ...
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bioRxiv - Biochemistry 2020Quote: ... The cell was digested in MP2 buffer (1.2M sorbitol, 20mM KH2PO4 pH 7.4) supplemented with 3mg zymolyase/g wet weight (Seikagaku Corporation) for 1 h at 30°C ...
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bioRxiv - Cell Biology 2022Quote: ... pH 5.8 the cells were resuspended in the same buffer containing 0.25 mg/ml Zymolyase 20T (Seikagaku Corp., Tokyo) and incubated for 2 h at 30 °C ...
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bioRxiv - Biochemistry 2023Quote: ... yeast cells were resuspended in 6.7 ml per g wet weight MP2 buffer (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase 20T from Seikagaku Biobusiness) and incubated for 1 h at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... washed with sorbitol buffer (1M sorbitol, 0.1M NaH2PO4, pH 8.0) and the cell wall was digested with Zymolyase 20T (Seikagaku Biobusiness Corporation) for about 35 min at 37° C ...
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bioRxiv - Biochemistry 2020Quote: ... and chondroitin sulfate E (CSE, Mw = 20 kDa) from squid cartilage (Seikagaku). N-desulfated HP (N-DeS HP ...
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bioRxiv - Microbiology 2020Quote: ... and chondroitin sulfate E (CS-E, Mw = 20 kDa) from squid cartilage (Seikagaku). Keratan sulfate (KS ...
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bioRxiv - Biochemistry 2020Quote: ... chondroitin sulfate D (CSD, Mw = 20 kDa) from whale cartilage (Seikagaku, Tokyo, Japan) and chondroitin sulfate E (CSE ...
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bioRxiv - Microbiology 2020Quote: ... chondroitin sulfate D (CS-D, Mw = 20 kDa) from whale cartilage (Seikagaku, Tokyo, Japan) and chondroitin sulfate E (CS-E ...
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bioRxiv - Cell Biology 2019Quote: ... clone 9 and clone 10 was purified using the Qiagen genomic DNA kit (Genomic-tip 20/G) using 30 OD equivalents of material following the manufacturer’s instructions after zymolyase treatment (Seikagaku). A PCR-free library was generated from 10 µg of gDNA and sequenced at the Beijing Genomics Institute (Hong Kong ...
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bioRxiv - Molecular Biology 2023Quote: ... VCAN (1:200, Mouse Anti-Versican Antibody 2B1, Seikagaku), and ELN (1:400 ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were resuspended in 500 μl SP buffer with 0.1% β-mercaptoethanol and 10 μl of 20 mg/ml Zymoylase-20T (Seikagaku). The cells were incubated with soft rotation for 20 min at room temperature (RT) ...
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bioRxiv - Molecular Biology 2023Quote: ... DCN (1:1500, Mouse Anti-Dermatan Sulfate Proteoglycan Antibody 6B6, Seikagaku), VCAN (1:200 ...