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Citations for GE Life Sciences :
1 - 50 of 1767 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 20 mM Tris-HCl pH 8 using PD-10 desalting columns (GE Healthcare). 10 μM CAD was hydroxylated by incubation with 10 μM FIH ...
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bioRxiv - Microbiology 2021Quote: ... The protein sample was exchanged into low salt buffer (20 mM Tris pH 8, 20 mM NaCl, 0.03% DDM) using a PD-10 column (GE Healthcare) and then injected onto a MonoQ column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... 20 mM Tris HCl pH 8.0) using Vivaspin filters with a 100 kDa molecular weight cutoff (GE Healthcare). Protein concentrations were determined using the Nanodrop method ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.0 prior to injection of 20 µL samples onto a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated in 1 M NaCl ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 20 mM MOPS-NaOH pH 6.7 and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... sample was desalted into Fab-storage buffer (20 mM HEPES pH 8.0, 0.1 M NaCl) using a pre-packed PD-10 column (GE Healthcare). Purified Fab30 was flash frozen stored in buffer supplemented with 10% glycerol.
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bioRxiv - Immunology 2021Quote: ... Samples were buffer exchanged into NMR buffer (20 mM sodium phosphate pH 7.0, 50 mM NaCl, 0.02% NaN3) using PD10 desalting columns (GE Healthcare). Samples were concentrated further to a volume of ~300 μL ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted samples were desalted into buffer (20 mM HEPES-Na+, pH 8.0, 0.1 M NaCl) using a pre-packed PD-10 column (GE Healthcare). Purified Fabs were flash-frozen and stored at −80 °C supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... The buffer was exchanged in assay buffer (10 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.1 % Tween-20) using PD10 gravity columns (GE Healthcare). The protein was concentrated using Amicon Ultra-0.5 10K Centrifugal Filter Devices (Merck Millipore) ...
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bioRxiv - Neuroscience 2019Quote: ... antibody-coated protein G–Sepharose beads (GE Healthcare; 20 μl) were added to the mixture and incubated on a shaker overnight at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... chip was prepared by amine coupling of anti-mouse IgG antibodies at pH 5.0 (Mouse antibody capture kit, GE Healthcare) according to the protocol described by manufacturer ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Cell Biology 2020Quote: ... was exchanged into labelling buffer (20 mM Tris-HCl pH 7.5, 100 mM NaCl) using size exclusion NAP10 columns (GE Healthcare). Protein-containing fractions were identified by protein electrophoresis and pooled ...
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bioRxiv - Biochemistry 2019Quote: ... For further purification the protein was diluted 1:1 with 20 mM HEPES KOH (pH 7.5) and loaded on a HiTrap Heparin HP (GE Healthcare) column ...
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bioRxiv - Biochemistry 2019Quote: ... and buffer exchanged to 10 mM phosphate buffer pH 7.4 containing 10 mM MgCl2 using 10 kD Vivaspin 20 ml columns (GE Healthcare). Samples were then loaded onto a CHT ceramic hydroxyapatite column (Bio-Rad Laboratories Inc.) ...
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bioRxiv - Biochemistry 2020Quote: ... The dialyzed protein was diluted 1:1 with 20 mM HEPES KOH (pH 7.5) and loaded on a HiTrap Heparin HP (GE Healthcare) column and washed with Buffer A (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... the purified proteins were dialyzed against TN200 buffer (20 mM Tris-HCl pH 7.5, 200 mM NaCl) using a HiTrap Dessalting column (GE Healthcare), and the protein was further purified by gel filtration chromatography on a HiLoad 16/600 Superdex 75 or HiLoad 16/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Purified Ycf1p NBD1 and NBD1-R were exchanged into the fluorescence buffer (20 mM Na3PO4, 2% glycerol, 150 mM NaCl, pH 7.3) by size exclusion chromatography (Superdex75, GE Healthcare). MgATP-bound and apo NBD1 and NBD1-R were generated by adding 2 mM MgATP or 2 mM EDTA ...
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bioRxiv - Plant Biology 2021Quote: ... in running buffer (20 mM Tris, pH 8, 150 mM NaCl, 1 mM DTT) supplemented with 0.05% of P20 surfactant (GE Healthcare). Anti-MBP murin monoclonal antibody (Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... and eluted in superdex buffer (20 mM Tris [pH 8.0], 300 mM NaCl, 1 mM TCEP) using the AKTA Explorer FPLC system (GE Healthcare). The protein was concentrated to 7.5 mg/ml.
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bioRxiv - Plant Biology 2020Quote: ... in a high-salt buffer containing 20 mM Tris-HCl (pH 8.0) and 50 mM NaCl in an ÄktaPrime or ÄktaExplorer system (GE Healthcare). The column was calibrated with bovine erythrocyte carbonic anhydrase ...
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bioRxiv - Biophysics 2022Quote: ... The excess Cy3-NHS was removed and the buffer was exchanged with a 20 mM MES buffer at pH 5.5 using a PD-10 column (GE Healthcare). The typical labeling efficiency ...
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bioRxiv - Biochemistry 2022Quote: ... and washed with 10 column volumes washing buffer (20 mM Tris, 500 mM NaCl, pH 7.8) using an AKTA explorer 100 (GE Healthcare). ARF-DBD proteins were eluted by 1 h incubation with 40 mM DTT in washing buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Samples collected from IMAC were exchanged into size exclusion chromatography (SEC) buffer (20 mM Na2HPO4, 150 mM NaCl, pH 7.4) using either a HiPrep 26/10 desalting column (GE Healthcare) or through multiple rounds of concentration and dilution using an Amicon Ultra-15 filter unit with a 10 kDa molecular weight cut-off ...
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bioRxiv - Developmental Biology 2020Quote: ... After the buffer was replaced with 20 mM sodium phosphate (pH 5.5) buffer containing 25 mM NaCl using a PD MiniTrap G-25 column (GE Healthcare), the digested sample was applied to a HiTrap Q HP column and every 1.5 ml was collected during the application and washing with the same buffer ...
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bioRxiv - Microbiology 2021Quote: ... and were eluted with CA loading buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM BME) using AKTAprime plus (GE Healthcare). CA monomer fractions were concentrated using Amicon Ultra-10K device (Merck Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... loaded complexes were further purified in a final size exclusion chromatography step (20 mM Tris-HCl pH 8.5, 150 mM NaCl) using a Superdex 200i column (GE Healthcare), before being used in down-stream applications.
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bioRxiv - Biochemistry 2020Quote: ... 250 μM TCEP and 20 mM sodium phosphate at pH 6.5 (ITC buffer) using a 5 mL HiTrap Desalting column (GE Healthcare). Likewise ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 ml of 20 µM purified Sup35NM were buffer exchanged into Fibril Forming Buffer (FFB - 20 mM sodium phosphate buffer pH 7.4, 50 mM NaCl) using a PD-10 column (GE Healthcare) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... L protein-containing fractions were pooled and diluted 1:1 with buffer C (20 mM HEPES(NaOH) pH 7.0) before loading on a heparin column (HiTrap Heparin HP, GE Healthcare). Proteins were eluted with Buffer A and concentrated using centrifugal filter units (Amicon Ultra ...
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bioRxiv - Immunology 2021Quote: ... the protein was diluted with 2-fold volume of 20 mM HEPES with pH 7.0 and loaded on HiTrap SP HP column (GE Healthcare) equilibrated with 100 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... L protein-containing fractions were pooled and diluted with an equal volume of buffer C (20 mM HEPES(NaOH) pH 7.0) before loading onto a heparin column (HiTrap Heparin HP, GE Healthcare). Proteins were eluted with buffer A and concentrated using centrifugal filter units (Amicon Ultra ...
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bioRxiv - Biochemistry 2020Quote: ... Elution buffer contained 500 mM imidazole and the eluted protein was immediately diluted with 20 mM Na-phosphate pH 6.5 followed by passing through an anion exchange chromatography column (HiTrap Q FF, GE Healthcare) and subsequent cation exchange chromatography (HiTrap SP FF ...
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bioRxiv - Biophysics 2022Quote: ... The refolding mixture was dialyzed at 4 °C against 20 mM MOPS-NaOH pH 6.7 to remove residual GdmCl and applied to a Resource S column (GE Healthcare) to separate FimCHisI from free FimCHis ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were dissolved in PBS pH 7.4 at 42 μM concentration and filtered with a Whatman Anotop 10 filter (20 nm cutoff, GE Healthcare) before starting the measurements ...
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bioRxiv - Microbiology 2023Quote: His-SUMO-FacZ127-145 and GpsB1-75 were thawed from frozen aliquots and separately buffer-exchanged into an identical buffer (20 mM HEPES, pH 7.6, 500 mM NaCl, 1 mM DTT) using PD10 de-salting columns (GE Healthcare). Gel filtration samples were made by mixing GpsB1-75 at 90 μM with SUMO-FacZ127-145 at 30 μM in 500 μL reactions ...
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bioRxiv - Biochemistry 2023Quote: ... in SEC buffer (20 mM Sodium-phosphate buffer pH 6.5, 50 mM NaCl) using an ÄKTA pure chromatography system (GE Healthcare). Eluted fractions were concentrated using Amicon® Ultra to 1 mM final concentration.
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bioRxiv - Biochemistry 2023Quote: ... and the external substrate was removed and exchanged into buffer (20 mM HEPES, pH 7.0 and 50 mM NaCl) using a PD10 desalting column (GE Healthcare) according to manufacturer’s instructions.
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bioRxiv - Biophysics 2023Quote: ... and buffer exchanged into a final buffer (20 mM Tris, pH 7.4, 500 mM NaCl) using a HiPrep Desalting column (GE Healthcare). The purified protein was flash-frozen in liquid nitrogen and stored at -20α C.
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bioRxiv - Cell Biology 2020Quote: ... The labeled protein was buffer exchanged into 20 mM Tris pH 7.4 and 50 mM NaCl and unreacted dye was removed using HiTrap Desalting Columns (GE Life Sciences). Labeled protein was then snap frozen for storage at −80°C ...
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bioRxiv - Biochemistry 2019Quote: ... were analyzed in aliquots of 50 μl each at a sample concentration of 2 mg/ml (in 20 mM Hepes pH 7.5, 150 mM NaCl) on a Superdex 200 10/300 increase column (GE Healthcare, Germany) at a column temperature of 35 °C and a flow rate of 0.7 mL/min ...
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bioRxiv - Biochemistry 2020Quote: ... Purified 6-His-PI3Kγ catalytic subunit17,18 was used as the soluble analyte and was injected at different concentrations over the immobilized GRK2 in SPR buffer (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% surfactant P-20, GE Healthcare Life Sciences, Biacore Inc.). Binding experiments were performed at 25°C in SPR buffer with a flow rate of 20 μl/min ...
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bioRxiv - Biophysics 2023Quote: ... The NP without MBP containing buffer was diluted to reach 150 mM NaCl concentration by adding 20 mM NaH2PO4 pH 8 and loaded on a heparin column (GE Healthcare) to remove bound bacterial RNA ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0, 0.15 M NaCl) using a HiPrep 26/10 column (GE Healthcare Life Sciences). The cleavage reaction was initiated by adding 6×His-tagged SUMO protease to a final concentration of ∼8 μg/mL to the protein solution ...
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bioRxiv - Biophysics 2024Quote: ... pure fractions were buffer-exchanged into the final buffer (20 mM Tris-HCl, pH 8, and 150 mM NaCl) using a HiPrep Desalting column (GE Healthcare) and stored at -20°C in the final buffer ...
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bioRxiv - Immunology 2020Quote: ... pH 8.0 (GE healthcare) to finish immobilization.
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bioRxiv - Pathology 2021Quote: ... pH 7.4 (GE Healthcare). The respective LRG1 proteins were immobilised directly onto a CM5 biosensor chip by amide coupling at pH 5.0 ...
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bioRxiv - Microbiology 2021Quote: ... pH 1.5 (GE Healthcare). The response units were recorded at room temperature and analyzed using the same software as mentioned above.
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bioRxiv - Microbiology 2021Quote: ... pH 1.5 (GE Healthcare). The apparent binding affinity (KD ...
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bioRxiv - Molecular Biology 2021Quote: ... was transferred from the purification buffer into the labeling buffer (Na-PO4 20 mM, pH 8.3) supplemented with OG (30 mM) using PD-10 columns (GE Healthcare, Solingen, Germany) according to the manufacturer’s protocol ...