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Citations for Qiagen :
1 - 50 of 759 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: 20 μL EB (10 mM TrisCl, pH 8.5, Qiagen) were added to the reaction ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.4 and stored at −20 °C in RNAlater solution (Qiagen). RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: ... pH 8) and 30 μl of 20 mg/ml Proteinase K (QIAGEN 19131) were added to the tissue/cell sample and incubated at 55°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... pH 8.0) containing 15 mg/mL of lysozyme and proteinase K solution (20 mg/mL, Qiagen), followed by an incubation with vortex and lysis steps described in the kit ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
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bioRxiv - Microbiology 2020Quote: ... pH 8.0) containing 15 mg/mL of lysozyme + 15 µL of proteinase K solution (20 mg/mL, Qiagen), and then incubated for 8–10 min ...
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bioRxiv - Immunology 2019Quote: ... and purified in buffer C (20 mM Tris, 100 mM NaCl, pH 7.5) by nickel-affinity chromatography (Qiagen) followed by size-exclusion chromatography on a Superdex 200 column in buffer D (20 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... 1.28 M sucrose, 40 mM Tris-Cl, pH 7.5, 20 mM MgCl2, and 4% Triton X-100; QIAGEN) and then digested by digestion buffer (QIAGEN buffer G2 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
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bioRxiv - Genomics 2019Quote: ... the fragmented DNA was concentrated to 20 µl in 100 mM NaPi buffer (pH 8.0) using QIAquick PCR purification kit (Qiagen). A 100-µl volume of 100 mM NaPi buffer containing 20 mM amine-PEG2-biotin (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... clarified samples were transferred to a 50 mL conical tube and supplemented with a final 20 mM imidazole pH 7.5 before batch-binding with Ni-NTA superflow resin (Qiagen) for ∼1 hr at 4°C ...
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bioRxiv - Immunology 2021Quote: ... or ectodomain buffer (20 mM Tris, pH 8.5, 500 mM NaCl) and applied to 10 ml Ni-NTA-Sepharose (Qiagen) columns ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was lysed with a lysis buffer (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, 20 mM EDTA, 1% SDS) and proteinase K (Qiagen) was added to samples to degrade proteins ...
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bioRxiv - Cell Biology 2023Quote: ... The expressed recombinant protein was solubilized in a denaturing buffer (6 M HCl-Guanidine, 20 mM Tris-HCl[pH 7.5] and purified by Ni-NTA (30210, QIAGEN) under denaturing conditions ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was diluted 3 times with buffer A (20 mM HEPES, pH 7.4, 200 mM NaCl, 1 mM Asp) and incubated with Ni-NTA resin (Qiagen) for 1 hour at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... The periplasmic fraction was filtered with a 0.22 um filter and incubated with 4 mL 50% Ni-NTA resin equilibrated in wash buffer (20 mM HEPES, pH 7.5, 150 mM NaCl, 40 mM imidazole) (Qiagen) per liter of initial bacterial culture ...
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bioRxiv - Biochemistry 2023Quote: ... 50 mg of tissue was homogenized in 500 µl Sucrose Buffer (250 mM sucrose, 20 mM imidazole, pH 7.0) using a TissueLyser II (Qiagen). Tissues from 3-4 mice were pooled together ...
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bioRxiv - Developmental Biology 2023Quote: ... solubilized in a denaturing buffer (6 M HCl-Guanidine, 20 mM Tris-HCl pH 7.5) and purified by Ni-NTA (QIAGEN) under denaturing conditions ...
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bioRxiv - Biochemistry 2022Quote: ... the best diffracting crystal for the 14-3-3σ Clu3/pS197 complex grew in condition G2 (0.2 M NaBr, 100 mM BisTris propane pH 7.5, 20% PEG 3350) of PACT screen (Qiagen, Hilden, Germany). Crystals were mounted in nylon loops directly from crystallization drops and flash frozen in liquid nitrogen without additional cryoprotection ...
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bioRxiv - Biochemistry 2023Quote: ... Tris–HCl 20 mM (pH 7.5)) by sonication and the His-tagged proteins were purified on a Ni-NTA column (Qiagen Inc.), eluted with imidazole in buffer A but complemented by ATP 1 mM + MgCl2 3 mM in the case of the Vibrio cholerae helicase ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were collected continuously into 50 mL conicals containing 20 mL RNAlater (saturated ammonium sulfate, 20mM EDTA, pH 5.2; QIAGEN #76106) and a stirring magnetic stir disc (V&P Scientific #772DP-N42-5-2) ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.5 (Qiagen, 19086) were added to DNA-bead mix and incubated in a Thermomixer C (Eppendorf ...
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bioRxiv - Genetics 2019Quote: ... pH 7.5-8.0 (Qiagen) for sequencing on Illumina platforms ...
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bioRxiv - Molecular Biology 2023Quote: ... 600 µl of lysis buffer (TES (0.1 M TRIS, 10 mM EDTA, 2% sodium dodecyl sulphate; pH 8) and Proteinase K (Qiagen, 20 mg/ml) in a 20:1 ratio ...
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bioRxiv - Genomics 2022Quote: ... pH 8.5 (EB from Qiagen). Libraries were amplified with PCR (17 cycles – 30 sec at 98°C ...
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bioRxiv - Microbiology 2019Quote: ... 9 mL 1M Tris HCl pH 7.5, 9 mL 0.5M EDTA pH 8.0, 11.25 mL 10% SDS, 22.5 mL Qiagen lysis reagent ...
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bioRxiv - Bioengineering 2021Quote: ... pH 7.4 using a TissueRuptor (Qiagen). The final homogenate weight was measured and converted to volume by using a homogenate density of 1 g·mL−1 ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 M Tris (pH 7.0) (QIAGEN), 30% PEG 3000 (QIAGEN ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from roots of 10-day-old plants grown at optimal pH (5.5) or exposed for six-hour to pH 7.5 media using the RNeasy Plant Mini Kit (Qiagen). Three independent biological replicates were performed ...
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bioRxiv - Microbiology 2021Quote: ... pH 8.5 buffer (Qiagen Sciences, Maryland, MD) was added to the concentrate to make up a total volume of 250 μL ...
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bioRxiv - Cancer Biology 2020Quote: ... pH 8.5 (Elution buffer EB, Qiagen, Germany). The length distribution of purified cDNA populations was determined on the Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Systems Biology 2020Quote: ... 20 (Qiagen). Paired-end reads were mapped with a length fraction of 0.4 ...
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bioRxiv - Bioengineering 2021Quote: ... 20 (Qiagen). Reads were mapped to the C ...
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bioRxiv - Biochemistry 2021Quote: ... 0.9 M KOAc [pH 4.8] (Qiagen N3 buffer). Centrifuge sample at 23°C for 10 minutes at 14,000 x g ...
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bioRxiv - Biochemistry 2023Quote: ... 0.9 M KOAc [pH 4.8] (Qiagen N3 buffer). Centrifuge sample at 23ºC for 10 minutes at 14,000 x g ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were washed with PBS-T (PBS1x/0.1% Tween 20) and incubated with mouse antibody against 6xHis-tag (1:2,000; Qiagen), rabbit antibody against GST-tag (1:10,000 ...
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bioRxiv - Microbiology 2022Quote: ... 20 μL of EDTA (final concentration 20 mM) and 1.3 μL of Proteinase K (20 mg/mL) (Qiagen) were added and incubated at 56 °C for 1.5 h ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 M Tris/HCl pH 8.5 (JCSG+ suite, Qiagen). Hanging drops of 1 ml protein at 2 mg/ml and 1 ml of precipitant (19% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... and added 5 volume PB including pH-indicator (Qiagen) and 200 μL sodium-acetate (3M ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 nmole (SI03046533, Qiagen) All Stars Negative Control siRNA ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 nmole (SI03650318, Qiagen)
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bioRxiv - Cell Biology 2020Quote: ... 20 µl Polyfect (Qiagen) and 1 µg plasmid were incubated for 5 minutes and the transfection mix was dropped to cover all cells without medium ...
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bioRxiv - Physiology 2022Quote: ... pH 7.5) using steel beads and a TissueLyzer II (QIAGEN). Tissue homogenates were rotated end-over-end for 1 h at 4°C before part of the homogenates were centrifuged at 16,000 g for 20 min at 4°C to generate tissue lysates (supernatant) ...
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bioRxiv - Biochemistry 2021Quote: ... pH 8.0) and applied to Ni-NTA Superflow resin (QIAGEN) or HisTrap FF crude (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Buffer EB (10 mM Tris-Cl, pH 8.5, Qiagen, Germany) was used ...
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bioRxiv - Microbiology 2023Quote: ... pH was neutralized employing 0.7 uL of Stop solution (Qiagen). Then ...
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bioRxiv - Biochemistry 2023Quote: ... Mutations in DH/PH-DEP1 were created using QuikChange (Qiagen) and confirmed by DNA sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... 2x Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5), 1x rCutsmart Buffer.
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bioRxiv - Biochemistry 2019Quote: ... Bound STAT3 proteins were then labeled with Alexa488-conjugated anti-6 × His antibodies (1 hr incubation, 1:20 dilution, Qiagen 35310). The array was washed and scanned using a GenePix 4400A scanner (Molecular Devices ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by incubations with a PBS-based protein binding mixture for 1 h and with Alexa488-conjugated anti-His antibody (1:20 dilution, Qiagen 35310) for 1 h ...