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Citations for anatrace :
1 - 47 of 47 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: A total of 4.0 μl of 20 μM TpCorC protein in 20 mM HEPES (pH 7.5) containing 0.03% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, USA) and either 150 mM NaCl or 150 mM KCl was mixed with 0.5 μl of 2 mM EDTA ...
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bioRxiv - Biophysics 2019Quote: ... A column was packed with the Sepharose beads and washed with Wash Buffer (20 mM HEPES pH 8.0, 150 mM NaCl, 2 mM TCEP) containing 0.006% DMNG (Anatrace). TRPV2 was eluted with Wash Buffer containing 0.006% DMNG and 3 mg/ml 1D4 peptide (GenScript USA ...
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bioRxiv - Biochemistry 2020Quote: ... The protein-bound resin was washed with 10 mM imidazole and eluted with imidazole (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 20 % glycerol, 0.1% DDM (Anatrace, USA), 1 M imidazole) ...
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bioRxiv - Biophysics 2022Quote: ... membrane pellets were homogenized in TBS (20 mM Tris-HCl, pH 7.5, 150 mM NaCl) and solubilized in TBS with 1% GDN (Anatrace), supplemented with protease inhibitors (1 mg/ml Leupeptin ...
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bioRxiv - Cell Biology 2021Quote: ROS of 50 bovine retinas were isolated as described previously and suspended in Extraction Buffer (20 mM BTP, pH 7.9, 10% glycerol, 300 mM NaCl, 1 mM TCEP) containing 2% LMNG (Anatrace) (Papermaster ...
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bioRxiv - Biophysics 2023Quote: ... and the cell pellets were resuspended in lysis buffer (50 mM HEPES-NaOH, pH 7.5, 500 mM NaCl, 5 mM MgCl2, 20 mM imidazole, 1mM CHAPS (Anatrace), 5mM 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2023Quote: ... Resin was loaded onto a column and washed with wash buffer (20 mM HEPES pH 8.0, 300 mM NaCl, 0.05 % DDM (Anatrace D310), 50 mM glutamate ...
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bioRxiv - Neuroscience 2023Quote: ... on a Superose 6 column in SEC buffer (20 mM HEPES pH 8.0, 150 mM NaCl, 0.05 % DDM detergent (Anatrace D310), and 50 mM glutamate).
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bioRxiv - Biophysics 2023Quote: ... and the resin was washed with 20 column volumes of GDN buffer (150 mM NaCl, 20 mM Tris pH 8.0, 0.01% GDN (glycol-diosgenin, Anatrace, GDN101)) ...
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bioRxiv - Biophysics 2022Quote: The pelleted membrane fractions were then solubilized in resuspension buffer (50 mM Tris/Mes, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5) containing 1% β-DDM (Anatrace) and stirred on ice for 60minutes ...
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bioRxiv - Biophysics 2023Quote: ... the lipid mixture was solubilized in 500 μL of 20 mM HEPES buffer (pH 7.5, 5%-w/v β-octylglucoside (Anatrace)) at the final lipid concentration of 7.5%-w/v ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane pellets were solubilized in 1 ml lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 10% glycerol, protease inhibitor cocktail, 2% UDM (Anatrace U300LA) on a rotary shaker for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were solubilized by douncing in solubilization buffer (20 mM HEPES pH 8.0, 300 mM NaCl, 2% DDM detergent (Anatrace D310S), 50 mM glutamate ...
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bioRxiv - Biophysics 2023Quote: ... which were solubilized in solubilization buffer (150 mM NaCl, 20 mM Tris pH 8.0, 1% n-Dodecyl-β-D-Maltopyranoside (Anatrace, D310) and 0.2% Cholesteryl Hemisuccinate Tris salt (Anatrace ...
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bioRxiv - Molecular Biology 2020Quote: ... + 0.1% Tween 20 (Anatrace)) and then probed with primary antibodies ...
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bioRxiv - Biophysics 2022Quote: ... pH 8.0) containing detergent of interest (0.5 % (w/v) n-dodecyl β-D-maltopyranoside (DDM, Anatrace) or 2 % (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... KLC1D/E synthetic peptides were diluted at 57 μM in PBS buffer pH 7.4 in the absence or presence of DPC (0.35%, Anatrace), DDM (0.05% ...
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bioRxiv - Cell Biology 2022Quote: ... Immobilization buffer was 10 mM HEPES buffer pH 7.4 containing 0.05% n-octyl-β-D-glucopyranoside (OGPS, Anatrace), 150 mM NaCl2 ...
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bioRxiv - Biophysics 2021Quote: ... 20 CV WB with 0.06 % (w/v) glyco-diosgenin (GDN) (Anatrace) and 20 CV WB with 0.02 % (w/v ...
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bioRxiv - Biophysics 2023Quote: ... 20 mM imidazole and 85 μM glyco-diosgenin (GDN, GDN101, Anatrace). The protein was eluted with 200 mM imidazole and digested with PreScission protease at 4 ℃ overnight to remove the C-terminal GFP-His10 tag ...
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bioRxiv - Biochemistry 2021Quote: ... and resuspended in 500 μl solubilization buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 1% (w/v) DDM (Anatrace), protease inhibitor cocktail set III (Merck Millipore) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were collected and centrifuged at 500 x g for 3 minutes, resuspended with lysis buffer (150 mM NaCl, 50 mM HEPES, pH 7.5, 1% (w/v) DDM (Anatrace), 1 mM PMSF) ...
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bioRxiv - Biochemistry 2021Quote: ... pre-equilibrated with size exclusion buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 1 mM DDM (Anatrace)) in line with a fluorescence detector (RF-20A ...
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bioRxiv - Biophysics 2022Quote: ... before being resuspended in buffer (50 mM Tris-HCl pH 7.4, 10% [v/v] glycerol, 1% [w/w] DDM [Anatrace] ...
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bioRxiv - Biochemistry 2019Quote: ... and then resuspended to 100 μl with solubilization buffer (85 mM NaCl, 17 mM Tris, pH 7.5 and 2% sodium dodecanoyl sarcosine (Anatrace)) ...
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bioRxiv - Biochemistry 2020Quote: ... This lipid cake was then resuspended in MSP standard buffer (25 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.5 mM EDTA) supplemented with sodium cholate (Anatrace, USA) to a final concentration of 100 mM ...
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bioRxiv - Plant Biology 2020Quote: ... and were suspended in a 25 mM MES buffer (pH 6.5) containing 1 M betaine at 0.3 mg Chl/mL and solubilized with 1.0% α-DDM (Anatrace, Maumee, OH) for 10 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed in ATF6 lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 1x protease inhibitor, and 1% LMNG (Anatrace NG322) for 15 min at 4°C as previously described68 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were solubilized by resuspending in buffer (50 mM Tris-HCl, pH 7.4, 10% [v/v] glycerol, 1% [w/w] dodecyl-β-D-maltoside [DDM, Anatrace] ...
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bioRxiv - Microbiology 2023Quote: ... 7[mM NMG (pH 8.0)) and solubilized by adding 40[mM DM (n-Decyl-β-D-Maltoside) (Sol-Grade from Anatrace). KtrB alone ...
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bioRxiv - Plant Biology 2023Quote: ... Tissue was lysed in 500 μl of lysis buffer (50 mM Tris-HCl pH 7.2, 10% v/v glycerol, 150 mM NaCl, 1% w/v DIBMA (Anatrace BMA101), with protease inhibitors (1% v/v ...
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction pellets were solubilized in solubilization buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2% n-dodecyl-beta-d-maltopyranoside (DDM) (Anatrace, USA) with 1 mM PMSF ...
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bioRxiv - Biochemistry 2023Quote: ... and receptor was subsequently extracted from membranes in a volume of 50 ml by addition of 2× solubilisation buffer (100 mM HEPES pH 7.5, 250 mM NaCl, 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.1% (w/v ...
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bioRxiv - Neuroscience 2019Quote: ... Cell powder was added to lysis buffer (50 mM Tris pH 8.0, 150 mM KCl, 60 mM dodecyl-β-D-maltoside (DDM, Anatrace Inc.), 100 μg/ml DNAse 1 ...
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bioRxiv - Biochemistry 2022Quote: ... diluted with 4 mL GTBS (50 mM Tris-HCl, 150 mM NaCl, 0.004% [w/v] glyco-diosgenin [GDN; Anatrace], pH 7.4) and concentrated to ~200 μL in the same concentrator ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 8 column buffers of GTBS (50 mM Tris HCl, 150 mM NaCl, 0.006% [w/v] glyco-diosgenin [GDN; Anatrace], pH 8.0). Ycf1p was eluted with 6 column volumes of GTBS containing 150 μg/mL 3×FLAG peptide ...
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bioRxiv - Biophysics 2019Quote: Membrane pellets were solubilized in resuspension buffer containing 20 mM β-DDM (Anatrace) and 0.5 mM DTT and incubated on ice with stirring for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... Unbound protein was removed by washing extensively with wash buffer (20mM Tris-Cl pH 8.0, 300mM NaCl, 30mM Imidazole, 10% glycerol, 5mM β-ME and 0.02% DDM (Anatrace, Cat. no: D310A) and eluted with 20mM Tris-Cl pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The cell pellet was re-dissolved and dounced in solubilization buffer (50 mM Tris-Cl, pH 8.0, 150 mM NaCl, 5 mM β-mercaptoethanol, 1% DDM (Anatrace, Cat. no. D310), 1 mM PMSF ...
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bioRxiv - Biochemistry 2023Quote: ... and washed with 20 CV of buffer A supplemented with 0.02% (w/v) GDN (Anatrace). The bound proteins of ERGIC-53 WT were eluted with overnight incubation of 2 CV elution buffer (buffer A supplemented with 0.02% GDN ...
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bioRxiv - Cell Biology 2020Quote: ... at 0.1-0.3mg/mL in EM buffer containing either 0.02% Tween-20 or 0.025% amphipol A8-35 (Anatrace) was applied to Quantifoil or UltrAufoil R1.2/1.3 grids (Quantifoil ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL of solubilization solution supplemented with the 2 % 6-Cyclohexyl-1-Hexyl-β-D-Maltoside (Cymal6, Anatrace) was added gently to the membrane solution and the sample incubated at RT for 30 min under moderate shaking ...
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bioRxiv - Biochemistry 2020Quote: ... protein sample was spun down at 290,000 x g for 20 min and mixed with either n-octyl-β-D-glucopyranoside (OG; Anatrace) or n-nonyl-β-D-glucoside (NG ...
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bioRxiv - Biochemistry 2022Quote: ... equilibrated with the FPLC buffer (20 mM HEPES, pH7.5, 150 mM NaCl) plus 1 mM (w/v) n-dodecyl-β-D-maltoside (DDM, Anatrace).
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction was homogenized on ice with Buffer B (20 mL/pellet) containing 1% dodecylphosphocholine (DPC, FOS-CHOLINE-12, Anatrace). Homogenized cells were ultracentrifuged at 100,000 x g for 30 minutes to remove the insoluble fraction ...
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bioRxiv - Microbiology 2024Quote: ... and pellets were dissolved in solubilization buffer (20 mM Tris, 150 NaCl, 15% glycerol, 3% (% w/v) n-dodecyl-β-D-maltoside (DDM; Anatrace)) ...