-
No products found
because this supplier's products are not listed.
Xu Cui, Yi Liu, Lih Feng Cheow,
bioRxiv - Immunology 2024
Quote:
... droplets were de-emulsified in 1H,1H,2H,2H-Perfluoro-1-octanol reagent (PFO, Sigma-Aldrich). Collected cells were stained with PE anti-mouse H-2Kb ...
-
No products found
because this supplier's products are not listed.
Jack Stubbs, et al.,
bioRxiv - Biochemistry 2024
Quote:
... a volume of 1H,1H,2H,2H-perfluoro-1-octanol (PFO, Merck) is added to the emulsion with gentle pipetting used to break the emulsion ...
-
No products found
because this supplier's products are not listed.
Sascha R.A. Alles, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione (NBQX, Tocris) and N,N,H,-Trimethyl-5-[(tricyclo[3.3.1.13,7]dec-1-ylmethyl)amino]-1-pentanaminiumbromide hydrobromide (IEM-1460 ...
-
No products found
because this supplier's products are not listed.
Supanat Phuangphong, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... Nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (Roche) and Fast Red (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Daniel T. Rademaker, et al.,
bioRxiv - Microbiology 2022
Quote:
... The surface of the master was treated with 1H,1H,2H,2H-perfluo-rooctyltrichlorosilane (Thermo Scientific) to promote the removal of elastomer ...
-
No products found
because this supplier's products are not listed.
Aniqa Tasnim, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline (NBQX, 5 μM; Abcam, ab120046) to block excitatory glutamatergic activity in slices ...
-
No products found
because this supplier's products are not listed.
Irene Julca, et al.,
bioRxiv - Plant Biology 2022
Quote:
... The medium was aspirated and fresh medium containing 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, premixed in 5:1 ratio, Promega) was added to the treated cells ...
-
No products found
because this supplier's products are not listed.
Anne D. Villela, et al.,
bioRxiv - Microbiology 2020
Quote:
... Membranes were washed three times with 1X PBS for 5 min and developed with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate in alkaline phosphatase buffer (Bio-Rad).
-
No products found
because this supplier's products are not listed.
Yu-Han Hung, et al.,
bioRxiv - Genomics 2020
Quote:
... 5′ de-capping with RNA 5′ pyrophosphohydrolase (RppH, NEB, M0356S), (3 ...
-
No products found
because this supplier's products are not listed.
Phillip Zhu, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The 3-nitro-tyrosine incorporation plasmid (pAcBac1-3-nitroTyr-A7, Addgene # 141173) was as previously described.35
-
No products found
because this supplier's products are not listed.
Hiroki Ikeda, et al.,
bioRxiv - Developmental Biology 2022
Quote:
A Smart-seq v4 3’ DE Kit (Takara Bio) was adapted to DRaqL as follows ...
-
No products found
because this supplier's products are not listed.
Joshua D. Kerkaert, et al.,
bioRxiv - Microbiology 2021
Quote:
... and 300μL of XTT solution was added to each well (0.5mg/mL XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] (VWR) with 25μM menadione in PBS) ...
-
No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
-
No products found
because this supplier's products are not listed.
Alexandre Jourdon, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Immunostaining was performed by incubating sections in blocking solution (PBS, 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
-
No products found
because this supplier's products are not listed.
Jasmin van den Heuvel, et al.,
bioRxiv - Cell Biology 2021
Quote:
... si-USP10-1 (5’-UCGCUUUGGAUGGAAGUUCUA-3’, Qiagen), si-USP10-2 (5’-UACGUCAACACCCAUGAUAGA-3’ ...
-
No products found
because this supplier's products are not listed.
Yun Deng, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... with 5-bromo-4-chloro-3-indolyl-phosphate nitro blue tetrazolium staining (Vector Laboratories, Burlingame, CA, USA). 10 ∼ 30 embryos were used for each probe ...
-
No products found
because this supplier's products are not listed.
Qin Ni, et al.,
bioRxiv - Biophysics 2024
Quote:
... (S)-nitro-Blebbistatin (1 µM, Cayman Chemical), W7 hydrochloride (20 µM) ...
-
No products found
because this supplier's products are not listed.
Basila Moochickal Assainar, et al.,
bioRxiv - Biophysics 2023
Quote:
... 0.5% NBD-PC (1-palmitoyl-2-(6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl)-sn-glycero-3-phosphocholine (Avanti Polar Lipids, Inc.)) ...
-
No products found
because this supplier's products are not listed.
Pau Perez Escriva, Tobias Fuhrer, Uwe Sauer,
bioRxiv - Microbiology 2021
Quote:
... Online mass calibration was performed using a second spray needle and a constant flow (5 ul/min) of reference solution containing purine and hexakis (1H, 1H, 3H -tetrafluoropropoxy)phosphazine (HP-0921, Agilent Technologies). Compounds were identified based on the retention time of chemical standards and their accurate mass (tolerance 20 ppm) ...
-
No products found
because this supplier's products are not listed.
Julie Chassagne, et al.,
bioRxiv - Pathology 2024
Quote:
... Sections were incubated with a goat monoclonal antibody against EEA1 (1/100; SICGEN, Cantanhede, Portugal) and Caveolin 3 (1/750, BD Biosciences, Pont de Claix, France) antibodies (for dog sections ...
-
No products found
because this supplier's products are not listed.
Katarzyna Zyla-Jackson, et al.,
bioRxiv - Immunology 2023
Quote:
... we incubated sequential de-paraffinized sections with anti-2’,3’-cyclic-nucleotide 3’-phosphodiesterase (CNPase) (mouse CNPase: 1:200 dilution; Biolegend, Inc.), anti-CD3 (rabbit ...
-
No products found
because this supplier's products are not listed.
Jianfang Li, et al.,
bioRxiv - Developmental Biology 2021
Quote:
DE-to-GT (3 d): DE intermediates were incubated in basal medium-I supplemented with 50 ng/ml FGF7 (PeproTech) for 3 days.
-
No products found
because this supplier's products are not listed.
Annemarie Lang, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... EMCN (Santa Cruz; clone V.5C7; 1:100; 2h at RT), COL10 (Abcam ...
-
No products found
because this supplier's products are not listed.
Hua Li, et al.,
bioRxiv - Cell Biology 2020
Quote:
... was amplified by PCR using the primers (5’-GGTTCCGCGTGGATCCATGTCTCATGCAGCCGAGCCA-3’ and 5’-GGAATTCCGGGGATCCTCAGGACTCCTCTTCAATGCTGA-3’) and cloned into BamHI site of pGEX-4T-1 expression vector (GE Healthcare) using In-Fusion HD Cloning System (Clontech) ...
-
No products found
because this supplier's products are not listed.
Buse Baran, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Guide RNA sequences “5-CGUGUCACGACGGAGUGGAU-3” and “5-AUGUUGUAUGUGCCUAUACG-3” were synthesized by GenScript by adding the Cas12a scaffold sequence (5-UAAUUUCUACUCUUGUAGAU-3 ...
-
No products found
because this supplier's products are not listed.
Marco S. Kaiser, et al.,
bioRxiv - Physiology 2021
Quote:
... 3’ and 5’ adaptors (Illumina) were ligated and the resulting product was reverse transcribed to generate cDNA by PCR ...
-
No products found
because this supplier's products are not listed.
Ole A.W. Haabeth, et al.,
bioRxiv - Immunology 2021
Quote:
... After washing wells were incubated with a 5-bromo-4-chloro-3⍰-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (R&D systems, mouse IFNγ Kit Cat # EL485). Plates were scanned and analyzed using ImmunoSpot Microanalyzer.
-
No products found
because this supplier's products are not listed.
Maria Blumenkrantz, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... followed by a second series of ethanol to Hemo-De Xylene Substitute dilutions (50% Hemo-De, 75% Hemo-De, 100% Hemo-De) (Electron Microscopy Sciences, 23412-01). The samples were then incubated in a 1:1 Hemo-De:Paraffin solution at 65 °C for an hour ...
-
No products found
because this supplier's products are not listed.
Masahito Yamagata, Wenjun Yan, Joshua R. Sanes,
bioRxiv - Neuroscience 2020
Quote:
... In situ hybridization using nitro-blue tetrazolium and 5-bromo-4-chloro-3′-indolyphosphate and double color in situ hybridization using TSA Plus (PerkinElmer) were performed as previously described (Yamagata et al.,1999 ...
-
No products found
because this supplier's products are not listed.
Ingo Amm, et al.,
bioRxiv - Cell Biology 2022
Quote:
... were mixed 1:1 with liposomes (5 mg/ml) and floated for 2h at 55,000 rpm in a TLS-55 rotor (Beckman) at 25 °C through a sucrose gradient ...
-
No products found
because this supplier's products are not listed.
Chang-Bin Jing, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... or either of two different gRNAs targeting exon 3 coding sequences of human TET2 (TET2-gRNA #1: 5’-TGGAGAAAGACGTAACTTC-3’ and TET2-gRNA #2: 5’-TCTGCCCTGAGGTATGCGAT-3’) were transfected with Nucleofector (Lonza). After transduction ...
-
No products found
because this supplier's products are not listed.
Parya Behzadi, et al.,
bioRxiv - Physiology 2024
Quote:
... membranes were stripped in 1 × NewBlot Nitro Western Blot Stripping Buffer (LI-COR, Lincoln, NE) for 10 min ...
-
No products found
because this supplier's products are not listed.
Marisa Nacke, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... de-waxed (Leica, AR9222) and epitope retrieval carried out ...
-
No products found
because this supplier's products are not listed.
Katarzyna Wacnik, et al.,
bioRxiv - Microbiology 2021
Quote:
... 3 µl of 1 M NaOH and 6 µl of Cell-Tak (Corning, 5% (w/v) in acetic acid ...
-
No products found
because this supplier's products are not listed.
Wei Huang, et al.,
bioRxiv - Genetics 2023
Quote:
... 14-3-3 polyclonal antibody (Proteintech, Cat#14503-1-AP), and beta tubulin antibody (Abways Technology ...
-
No products found
because this supplier's products are not listed.
Jennifer Hua, et al.,
bioRxiv - Neuroscience 2022
Quote:
... BML-P137) for 1h and 2h before reading fluorescence (ex 365, em 440) on a plate reader (Flexstation 3, Molecular Devices,). For cathepsin B activity assessment by microscopy ...
-
No products found
because this supplier's products are not listed.
Aaron T. Crain, et al.,
bioRxiv - Genomics 2024
Quote:
... Membranes were blocked in 5% milk in TBS- Tween for 1h prior to incubation with α-GFP (1:1000, Rockland) or α-Set8 (1:1000 ...
-
No products found
because this supplier's products are not listed.
Carmen Mirabelli, et al.,
bioRxiv - Microbiology 2022
Quote:
... the 2h condition was harvested in TRI Reagent (Zymo Research, R2050-1) and the rest of infected-HIE were resuspended in BME and plated in a pre-warmed 24-well plate (3x 10uL drop/condition) ...
-
No products found
because this supplier's products are not listed.
Erin M. Euliano, et al.,
bioRxiv - Bioengineering 2024
Quote:
... 50 µL of each diluted sample added to 5 mL tubes (Eppendorf, Hamburg, DE), resulting in 1 million cells per flow sample.
-
No products found
because this supplier's products are not listed.
John K. Mich, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the following primers were used: (5’-GGTTTCCCGCAGAACCTGAA-3’) and (5’-CCATCGCTCGACCAGTTTAGT-3’) (Jackson Laboratories)
-
No products found
because this supplier's products are not listed.
Gladiola Goranci-Buzhala, et al.,
bioRxiv - Cell Biology 2020
Quote:
... coated culture dishes for 1h at 37°C with 5% CO2 using mTeSR1 medium (STEMCELL technologies). Cultures were routinely tested for mycoplasma contamination using MycoAlert Kit (Lonza) ...
-
No products found
because this supplier's products are not listed.
Or-Yam Revach, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Luminescence was read every 10 min for 1h using Cytation 5 microplate reader and Gen5 software (BioTek). Relative NAD(H ...
-
No products found
because this supplier's products are not listed.
Lyra O. Randzavola, et al.,
bioRxiv - Immunology 2022
Quote:
... and treated for 2h with ATP (2.5mM, Invivogen). Supernatants were harvested and subjected to Caspase-Glo 1 inflammasome assay following manufacturer’s protocol (G9951 ...
-
No products found
because this supplier's products are not listed.
Stefano Antonucci, et al.,
bioRxiv - Neuroscience 2024
Quote:
... sections were incubated in BB for 2h and with primary antibodies (anti-misfSOD1 1:1000, Médimabs MM-0070; anti-VAChT 1:500, Synaptic Systems, 139105 ...
-
No products found
because this supplier's products are not listed.
Kemin Tan, et al.,
bioRxiv - Immunology 2023
Quote:
... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
-
No products found
because this supplier's products are not listed.
Ana C. Sias, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and female (GRABDA2h, N =1; GRABDA2m, N = 3) Long Evans rats (Th-cre-littermates, N = 5; Gad-cre-, N = 2; Charles River Laboratories, N = 3) aged 7-9 weeks at the time of surgery were used to record dopamine release in the BLA across Pavlovian trace conditioning ...
-
No products found
because this supplier's products are not listed.
Nelson García-Vázquez, et al.,
bioRxiv - Biochemistry 2024
Quote:
... LC-3 (1:5,000, Novus Biological NB100–2220), p21 (1:1,000 ...
-
No products found
because this supplier's products are not listed.
Haribaskar Ramachandran, et al.,
bioRxiv - Genomics 2021
Quote:
... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
-
No products found
because this supplier's products are not listed.
Stephen Abini-Agbomson, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Cells were incubated in blocking solution (3% BSA in TBS-Tween 0.1%) for 1h with primary antibody (anti-53BP1, Rabbit polyclonal, Bethyl #A300-272A) diluted in blocking solution overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Amy R. Rappaport, et al.,
bioRxiv - Immunology 2021
Quote:
... Membranes were blocked for 1h in 5% skim milk in TBST and then probed with an anti-S2 mouse monoclonal antibody (GeneTex) at a 1:1000 dilution for 2h-overnight ...