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Citations for Stemcell Technologies :
1 - 50 of 1185 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... coated culture dishes for 1h at 37°C with 5% CO2 using mTeSR1 medium (STEMCELL technologies). Cultures were routinely tested for mycoplasma contamination using MycoAlert Kit (Lonza) ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were cleared for 1h in Optiprep (Stem Cell Technology). For dorsal imaging of the developing head ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3–5 days with ReLeSR (STEMCELL Technologies). All cells used had a normal diploid karyotype.
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bioRxiv - Cell Biology 2021Quote: ... for 5 min followed by a 3 min treatment with 5 mg/mL dispase (Stem Cell Technologies, 07913) and 1 mg/mL DNase I (Sigma ...
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bioRxiv - Genomics 2020Quote: Unsorted floating cells were taken from d2-3 of the blast culture and 5×103 cells were seeded in 1 ml MethoCult (M3434 STEMCELL Technologies) per dish ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 3-5 days using ReLeSR™ (#05872; Stem Cell Technology).
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bioRxiv - Cancer Biology 2024Quote: ... To obtain single cells the tissue was centrifuged at 450 x g for 3 min and the cell pellet was resuspended in 1 mL Dispase 5 U (Stemcell Technologies, 07913) with 100 μL of DNase I 1 mg/mL (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... and interleukin-3 (IL-3; 1 ng/mL, first day only; Stemcell Technologies; Canada). Cell density was maintained between 0.7-2×106 cells/mL by daily feeding with fresh expansion medium.
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bioRxiv - Immunology 2021Quote: De-identified peripheral blood units were purchased from StemCell Technologies (Vancouver, Canada). Peripheral blood samples from 25 healthy donors (11 male and 14 female ...
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bioRxiv - Cell Biology 2024Quote: ... 1 ng/mL IL-3 (STEMCELL Technologies, 78040) and 1% Penicillin-Streptomycin (Gibco 15140122) ...
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bioRxiv - Genomics 2021Quote: ... DE cultures were generated using the StemDiff Definitive Endoderm Kit (Stem Cell Technologies) as per manufacturer’s protocol for 5 days ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μM PD03259010 and 3 μM CHIR99021 (STEMCELL Technologies) and 100 U/ml penicillin ...
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bioRxiv - Developmental Biology 2022Quote: ... the media was changed to pre-warmed 37°C Medium DE-1 (STEMdiff™ Endoderm Basal Medium containing Supplement MR and Supplement CJ, Stem Cell Technologies). One well of seeded cells was left untreated as negative control (data not shown) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged every 3-5 days after dissociation with ReLeSR(Stem Cell Technologies, 100-0483). The medium was supplemented with Rho-associated protein kinase (ROCK ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5 mg ml-1 dispase (STEMCELL Technologies) and 1 mg ml-1 DNAse (Sigma ...
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bioRxiv - Immunology 2021Quote: Human peripheral blood from de-identified healthy donors was purchased from STEMCELL Technologies (Vancouver, Canada), which collects and distributes de-identified human blood products with consent forms ...
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bioRxiv - Cancer Biology 2024Quote: ... Common Rule (Category 4) or 2) de-identified donor leukapheresis products obtained from StemCell Technologies. CD3+ T-cell populations were isolated using RosetteSep Human T Cell Enrichment Cocktails (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Common Rule (Category 4) or 2) de-identified donor leukapheresis products obtained from StemCell Technologies. CD3+ T-cell populations were isolated using EasySep Human T Cell Isolation Kit (StemCell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Split NPCs 1:3 every week with accutase (STEMCELL Technologies; 7920). NPCs were further differentiated into human astrocytes by seeding dissociated single cells at 15,000 cells/cm2 density on Matrigel-coated plates in Astrocyte medium (ScienCell ...
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bioRxiv - Neuroscience 2023Quote: ... Six-well plates coated for at least 2h with 10µg/mL of Cell Adhere Laminin 521 (Stem Cell Technology, 200-0117) diluted in DPBS containing calcium and magnesium (Corning ...
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bioRxiv - Developmental Biology 2024Quote: ... and 5 μl DNase (1 mg/ml Stemcell Technologies) at 37°C ...
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bioRxiv - Immunology 2024Quote: Human peripheral blood leukopaks from de-identified healthy donors were purchased from STEMCELL Technologies (Vancouver, Canada). CD4+ T cells and CD8+ T cells were enriched using the EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... WA09 cells were differentiated to definitive endoderm (DE) using the STEMdiffTM Definitive Endoderm Kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... mTeSR was changed every other day and cells were passaged every 3–5 days using ReLeaSR (StemCell Technologies #05872). All cell cultures were maintained in a humidified incubator with 5% CO2 at 37°C ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... cells were passaged at 1:3 using Accutase (STEMCELL Technologies, Cat#07920) and cultured in NPC medium on Matrigel coated plates onwards.
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bioRxiv - Cell Biology 2021Quote: Endodermal cells were induced from cynIPS cells using STEMdiff Definitive Endoderm (DE) Kit (Stem Cell Technologies, 05110). CynIPS cells were seeded onto a 24-well plate (TPP ...
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bioRxiv - Cell Biology 2023Quote: ... final concentrations of either 3 µM CHIR99021 for definitive hematopoietic differentiation or 3 µM IWP2 (Selleckchem, S7085) and 1 ng/ml activin A (Stemcell Technologies, 78001) for primitive hematopoietic differentiation were added to the media for 30 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte specification was completed by 3 days of culture in RPMI B27-media with 5 μM IWP-4 (STEMCELL Technologies), followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 days post-transfection in 1 ml STEMdiff Neural Progenitor Medium (Stemcell Technologies) cells were washed by DPBS and 2 wells of the plate were put together and collected by centrifugation at 10.000 rpm for 2 min generating 3 biological replicates ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were passaged 1:10 every 3 days using ReLeSR (StemCell Technologies #05873). Cells were maintained in a humidified incubator at 37°C with 5% CO2.
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bioRxiv - Systems Biology 2024Quote: ... we passaged the cells at 1:3 using Accutase (STEMCELL Technologies, Cat#07920) and cultured cells in NPCs medium onwards.
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bioRxiv - Physiology 2024Quote: ... coated with GelTrex (Fisher cat. A1413302; 1h at 37°C) and maintained in mTeSR media (Stem Cell Technologies cat. 85850). Rock inhibitor 10μM Y-27632 was added at seeding and after passages.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Phase 1 (S1, 3 days): Day 1 S1 medium + 100 ng/mL Activin A (StemCell Technologies, Cat# 78001) + 3 μM Chir99021 (Stemgent ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... cells were maintained on 6-well plates in a humidified incubator at 37°C with 5% CO2 and passaged every 3-4 days (at ∼80% confluence) using ReLeSR (Stemcell Technologies), following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: MSCs at passages 1 or 3 were seeded in wells of a 12-well plate containing 3 ml Mesencult (Stem Cell Technologies, Vancouver, B.C.) each at 8.0×104 ...
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bioRxiv - Bioengineering 2023Quote: ... containing 300 cells and with 10ng/ml IL-3 (+IL-3 treatment) or without IL-3 (-IL-3 treatment) were plated in a well of a SmartDish (StemCell Technologies, Seattle, WA, USA) in duplicates ...
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bioRxiv - Genetics 2020Quote: ... to each well for 1h at room temperature) containing 0.5ml complete ReproTeSR medium (ReproTeSR basal medium with 25X ReproTeSR supplement) (Stem Cell Technologies). After 24h incubation ...
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bioRxiv - Neuroscience 2022Quote: ... and Neurotrophin-3 (NT-3; 20 ng/ml, Stemcell technologies) and the culture medium was changed every 3-4 days ...
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bioRxiv - Cell Biology 2020Quote: ... Neural rosettes were derived between day 3 to day 5 by addition of STEMdiffTM Neural Rosette Selection Reagent (05832, Stem Cell Technologies) for 45 mins-1 hr at 37oC ...
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bioRxiv - Physiology 2024Quote: ... Cells were utilized between passages 20 and 80 and passaged every 3–5 days at a confluency of 85–100% using Accutase (STEMCELL Technologies, #07920). The cardiac differentiation protocol was performed as previously described 49 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by basic medium containing 5 μM IWR-1-endo (STEMCELL Technologies) for another 48 h ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) from de-identified healthy donors were purified from leukapheresis products purchased from Stem Cell Technologies using EasySep Direct Human PBMC Isolation Kits (Stem Cell Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
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bioRxiv - Biophysics 2020Quote: ... were grown and maintained at 37°C in hypoxic conditions (3% O2, 5% CO2) in NeuroCult™ NS-A Proliferation medium (STEMCELL Technologies, 05751) supplemented with 1% Heparin Solution (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... IPSCs were cleaned and 1/3 dish was gently dissociated with dispase (Stemcell Technologies # 07923) for 12-15 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were split by dissociating to small clusters and replating at a 1:3-1:4 ratio using ReLeSR (StemCell Technologies) once cell clusters reached ∼70% coverage of the culture surface ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% FBS and 1:10 dilution of collagenase/hyaluronidase (STEMCELL Technologies) at 37°C for 3 hr ...
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bioRxiv - Bioengineering 2022Quote: ... present in human umbilical cord blood by culturing whole cord blood mononuclear cells from de-identified donors in EC-Cult-XF medium (STEMCELL Technologies) supplemented with 2 mg/mL heparin (#07980 ...