-
No products found
because this supplier's products are not listed.
Giulia Birolini, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 400 ng of D7-24S-hydroxycholesterol (D7-24S-OHC) (Avantipolar Lipids) and 100 µg of epicoprostanol (Sigma-Merck) as internal standards ...
-
No products found
because this supplier's products are not listed.
James Pelletier, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 5 micromols sodium sulfo-NHS and 5 micromols 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Thermo Fisher #22980) in 10 μL of DMSO ...
-
No products found
because this supplier's products are not listed.
Elizabeth C. Nowak, et al.,
bioRxiv - Immunology 2024
Quote:
... Transwell plates (24 wells with 3 μm pore membrane; Corning, Corning, NY) were loaded with 20 ng CXCL2 in the bottom compartment and 6×105 neutrophils in the top compartment ...
-
No products found
because this supplier's products are not listed.
Giulia Birolini, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 400 ng of D7-24S-hydroxycholesterol (D7-24S-OHC) (Avantipolar Lipids) and 100 µg of epicoprostanol (Sigma-Merck) as internal standards ...
-
No products found
because this supplier's products are not listed.
Gergő Porkoláb, et al.,
bioRxiv - Bioengineering 2023
Quote:
... supplemented with Y-27632 (Tocris, cat# 1254, 5 µM, 24 h, day –3). On days 0 and 1 ...
-
No products found
because this supplier's products are not listed.
Johanna Hol Fosse, et al.,
bioRxiv - Microbiology 2023
Quote:
... and homogenised with 3-5 mm steel beads in a TissueLyser II (Qiagen, 24 Hz, 2 × 3 min). 350 μL lysed blood or 200 μL lysed head kidney were transferred to a Magna Pure 96 Processing Cartridge (Roche) ...
-
No products found
because this supplier's products are not listed.
Arjun Bhatta, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and tRNAGln with 5 nt-long 5’ leader and same 24 nt-long 3’ trailer as tRNATyr (5–tRNAGln–24) – were transcribed in reactions containing 1x T7 RNA polymerase reaction buffer (NEB), 0.001% (w/v ...
-
No products found
because this supplier's products are not listed.
Ru Jia, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... 5 mM Na3VO4) and 3∼4 mL of 0.5-mm diameter glass beads using FastPrep-24 (MP Biomedicals). Proteins were immunoprecipitated with GFP-Trap A beads (Chromoteck ...
-
No products found
because this supplier's products are not listed.
Patrick B. Schwartz, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Apoptosis was measured by detecting Caspase 3/7 activity after 24 hours of treatment using the Caspase-Glo 3/7 Assay System (Promega) on a BMG CLARIOstar plate reader ...
-
No products found
because this supplier's products are not listed.
Kristina Ulicna, et al.,
bioRxiv - Biophysics 2020
Quote:
... 3 ⨯ 104 cells / cm2 in 24-well plates (ibidi). Imaging was started 2–3 h after seeding ...
-
No products found
because this supplier's products are not listed.
Matthew Prideaux, et al.,
bioRxiv - Cell Biology 2020
Quote:
... After 24 h cells were transfected with an eGFP vector (3 μg; Clontech) using Fugene-6 HD reagent according to the manufacturer's protocol ...
-
No products found
because this supplier's products are not listed.
Qirui Zhao, et al.,
bioRxiv - Bioengineering 2023
Quote:
... coverslips (24 × 24 mm; Cat.# 48393230, VWR International), magnetic beads (diameter 70–130 nm ...
-
No products found
because this supplier's products are not listed.
Huan Du, et al.,
bioRxiv - Neuroscience 2021
Quote:
... targeted to mouse PGRN exon 1 (oligos with 5’-cacc gGCTCCCTGGGAGGCATCTGG-3’ and 5’-aaac CCAGATGCCTCCCAGGGAGCc-3’ or 5’-cacc gCGGACCCCGACGCAGGTAGG-3’ and 5’-aaac CCTACCTGCGTCGGGGTCCGc-3’ were ligated to pLenti-CRISPRv2 (Addgene)) or Cas9 only ...
-
No products found
because this supplier's products are not listed.
Roberto Amigo, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... or Adenosine-5’-O-(3-thiotriphosphate) (Roche, 11162306001), 1.5 μL of TF buffer ...
-
No products found
because this supplier's products are not listed.
Lien Van Hoecke, et al.,
bioRxiv - Immunology 2024
Quote:
... Merck)-coated Thincerts (3 µm, translucent, 24-well plate, 662631, Greiner Bio-One). To purify the cell culture ...
-
No products found
because this supplier's products are not listed.
Jordan Cheng, et al.,
bioRxiv - Genomics 2021
Quote:
... and purified with Clarefy Purification Beads (Claret Bioscience, CBS-K250B-24, CBS-UM-24, CBS-UR-24, CBS-BD-24). Since there is currently no optimized method to measure ussscfDNA ...
-
No products found
because this supplier's products are not listed.
Yavuz F Yazicioglu, et al.,
bioRxiv - Immunology 2023
Quote:
... 130-120-662) for 3 days in 24-well plates coated with 3μg/ml anti-CD3 (145-2c11; Biolegend) and 3μg/ml anti-CD28 (37.51 ...
-
No products found
because this supplier's products are not listed.
Shuhei Yoshida, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 1 mM adenosine 5’-O-(3-thiotriphosphate) (ATPγS, ab138911, abcam) was used instead of ATP for the kinase reaction ...
-
No products found
because this supplier's products are not listed.
Heidi M. Blank, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The tert-butyldimethylsilyl (TBDMS) derivatized samples were analyzed with a gas chromatograph connected to a mass spectrometer (GC-MS) (Agilent 7890A and 5975C inert XL Mass Selective Detector) ...
-
No products found
because this supplier's products are not listed.
Spencer S. Gang, et al.,
bioRxiv - Immunology 2022
Quote:
... col-12p::mCherry] transgene were treated with 24 mM ethyl methanesulfonate (EMS) (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Lucy G Thorne, et al.,
bioRxiv - Microbiology 2020
Quote:
... and 100 μg/ml of Zeocin™ (Invivogen).Caco-2 and Calu-3 cells were stimulated for 24 h with media containing TLR4 agonist Lipopolysaccharide (LPS) (Peprotech), the TLR3 agonist poly I:C (Peprotech ...
-
No products found
because this supplier's products are not listed.
Yuka Takemon, et al.,
bioRxiv - Systems Biology 2020
Quote:
... 8 pools of 24 randomized libraries were sequenced in 3 lanes at 100 bp single-end on the HiSeq 2500 (Illumina) using TruSeq SBS Kit v4 reagents (Illumina).
-
No products found
because this supplier's products are not listed.
Robert Hardt, et al.,
bioRxiv - Cell Biology 2023
Quote:
... in the 24-fraction setup using pI 3-10 IPG strips (GE Healthcare) for 50 kVh ...
-
No products found
because this supplier's products are not listed.
Carlos J. Garcia, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Authentic standards of 3,7-Dihydroxy-5-cholan-24-oic Acid (chenodeoxycholic acid) and 3-Hydroxy-11-oxo-5-cholan-24-oic Acid (3-oxo-chenodeoxycholic acid) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). The N-(3α,7α,12α-trihydroxy-5β-cholan-24-oyl)-glycine (glycocholic acid) ...
-
No products found
because this supplier's products are not listed.
Rahul Bhattacharjee, et al.,
bioRxiv - Cell Biology 2022
Quote:
... cells were grown in YES at 32°C to mid-log phase and treated with 4-amino-1-tert-butyl-3-(3-methylbenzyl)pyrazolo[3,4-3]pyrimidine (3MB-PP1) (Toronto Research Chemical; A602960 or Cayman Chemical; 17860) at a final concentration of 15 µM for 30 min ...
-
No products found
because this supplier's products are not listed.
Yuting Wang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 2-3 months young and 22-24 months old male C57BL/6 mice were used (Jackson Lab #00664). For HSC transplantation ...
-
No products found
because this supplier's products are not listed.
Jana Hucklenbroich, et al.,
bioRxiv - Plant Biology 2021
Quote:
... thaliana (5’-CAGGCGGTGGAAACTACCAAG-3’ and 5’-TACAGCACTGCACGGGTCGAT-3’) and R129_E (5’-CGAGCTAATCTCCAAAAGCCATC-3’ and 5’-TGACCCTACCGTGGTTAGCTG-3’) with iQ SYBR Green Supermix (BIO-RAD), as described previously (Garrido-Oter et al. ...
-
No products found
because this supplier's products are not listed.
Christian-Marco Klask, et al.,
bioRxiv - Microbiology 2021
Quote:
... and centrifuged for 3 min at 13806 rpm (Centrifuge 5424, FA-45-24-11, Eppendorf) before use ...
-
No products found
because this supplier's products are not listed.
Bruno Vaz, et al.,
bioRxiv - Immunology 2020
Quote:
Human T cells (2-5 x 106) were transfected with 3 μg of TERT KO CRISPR/Cas9 plasmid (h) (sc-400316, Santa Cruz) according to the manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Takefumi Kimura, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... small mothers against decapentaplegic (SMAD) 2/3 (D7/G7) (Cell Signaling Technology, #8685, 1:1,000), Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425 ...
-
No products found
because this supplier's products are not listed.
Cagney Coomer, et al.,
bioRxiv - Neuroscience 2023
Quote:
Larvae were anesthetized with 0.02% ethyl 3-aminobenzoate methanesulfonate salt (Tricane, (E10521, Ethyl 3-aminobenzoate methanesulfonate) and mounted in 1% low melting point agarose (50100, Lonza) in a 60 mm x 15 mm petri dish ...
-
No products found
because this supplier's products are not listed.
James M Kelvin, et al.,
bioRxiv - Bioengineering 2023
Quote:
OCI-AML5 cells were cultured in 24-well plates (3×105 cells/well) in cRPMI media (FBS from R&D Systems; Minneapolis ...
-
No products found
because this supplier's products are not listed.
Lauren Rylaarsdam, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 3 million iPSCs per cell line were plated into 1 well of an AggreWell™800 24-well plate (Stem Cell Technologies, 34811) with 2 mL STEMdiff™ Neural Induction Medium + SMADi + 10 µM Y-27632 ...
-
No products found
because this supplier's products are not listed.
Mack B. Reynolds, et al.,
bioRxiv - Immunology 2024
Quote:
Bacterial burden in MRSA /ux-infected mouse ears was monitored every 24 h for 3 or 7 consecutive days using an In Vivo Imaging System (IVIS) Spectrum (Perkin Elmer). Bacterial burden was quantified as the bioluminescence (radiance ...
-
No products found
because this supplier's products are not listed.
Yuting Liu, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and 24 h later puromycin (InvivoGen) was used to select clones for two weeks ...
-
No products found
because this supplier's products are not listed.
Eve T. Beauchemin, et al.,
bioRxiv - Microbiology 2022
Quote:
All bacteria were incubated anaerobically in the 24-well plate at 37°C for 3 hours in an Epoch 2 Microplate Spectrophotometer (BioTek), with optical density measured at 600 nm (OD600) ...
-
No products found
because this supplier's products are not listed.
Yuki Ogawa, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Swiss Webster mice were purchased from Charles River (Cat# CRL:24; RRID: IMSR_CRL:24). All experiments were performed in compliance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Baylor College of Medicine ...
-
No products found
because this supplier's products are not listed.
Anthoni M. Goodman, et al.,
bioRxiv - Neuroscience 2020
Quote:
... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
-
No products found
because this supplier's products are not listed.
Dorota Kmiec, et al.,
bioRxiv - Microbiology 2021
Quote:
... were seeded in 24-well plates and transfected the following day using PEI MAX (3:1 PEI to DNA ratio; Polysciences) with 500 ng pHIV-1 and 0-250 ng pcDNA3.1 protein expression construct ...
-
No products found
because this supplier's products are not listed.
Hiroshi Ichise, et al.,
bioRxiv - Immunology 2021
Quote:
... an 430/24 (Olympus) excitation filter ...
-
No products found
because this supplier's products are not listed.
Erez Yirmiya, et al.,
bioRxiv - Genetics 2023
Quote:
... chip activation was made with a freshly prepared mixture of N-hydroxysuccinimide (50 mM in water) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (195 mM in water) for 7.5 min in DPBS (Sartorius, SKU 02-023-5A) (flow rate of 10 μL/min) ...
-
No products found
because this supplier's products are not listed.
Élora Midavaine, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 0.3% Triton X-100 (24 hours, 37°C) followed by peroxidase-conjugated secondary antibody (1:200 biotinylated anti-rabbit Vector Labs #BA-1000) and incubated in Elite ABC solution ...
-
No products found
because this supplier's products are not listed.
Reimi Tada, et al.,
bioRxiv - Developmental Biology 2023
Quote:
Tadpoles and froglets anesthetized with 0.025% or 0.05% ethyl-3-aminobenzoate were observed under a fluorescence stereomicroscope (Nikon SMZ18) with a 0.5x objective lens using a GFP longpass filter (if in a fluorescent view ...
-
No products found
because this supplier's products are not listed.
Valentina Gandin, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... using the following two primer sequences (T7 Forward: 5’-TAATACGACTCACTATAGCGTCATC-3’; Reverse: 5’-TTGTCGCACGTTCGGTGTCG-3’) and purified with DNA Clean and Concentrator-5 Kit (Zymo Research, 11-302). dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB ...
-
No products found
because this supplier's products are not listed.
Nioosha Nekooie-Marnany, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Cy-3 or Cy-5 (Jackson Immunoresearch Laboratories), and processed for DAPI or Hoechst staining to visualize cells’ nuclei before mounting in ImmuMount medium (Shandon) ...
-
No products found
because this supplier's products are not listed.
Aniruddha Das, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A 3×5 mm2 craniotomy was drilled (Omnidrill35, World Precision Instruments) over an area covering the monocular and binocular primary visual (V1m and V1b ...
-
No products found
because this supplier's products are not listed.
Kamal Mandal, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and 5 mg of C18 solid phase (3 μm, Durashell, Phenomenex). The HpHt column was sequentially washed with a series of 3 different solvents/solutions namely methanol ...
-
No products found
because this supplier's products are not listed.
Cody James Hastings, et al.,
bioRxiv - Microbiology 2023
Quote:
... for 24 hours in a microtiter plate reader (Beckman DTX880) at 37°C.
-
No products found
because this supplier's products are not listed.
Paul Cheng, et al.,
bioRxiv - Cell Biology 2020
Quote:
... was recorded after 24□h using a SpectraMax L luminometer (Molecular Devices). Relative luciferase activity (firefly/Renilla luciferase ratio ...
-
No products found
because this supplier's products are not listed.
Timm O. Koller, et al.,
bioRxiv - Biochemistry 2022
Quote:
Grids (Quantifoil R3/3 Cu300 with 3 nm holey carbon) were glow discharged and 4 µL of sample (8 OD260/mL ...