Labshake search
Citations for Takara Bio :
4851 - 4900 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... The isolation of the upstream genome sequences of gnrh1 and gnrh3 of piranha was performed using the Universal GenomeWalker ™ kit (Takara Bio USA) according to the instructions ...
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bioRxiv - Plant Biology 2023Quote: The cDNA prepared from 10-day-old OsbZIP48OE seedlings was used to perform 5’ and 3’ RACE using SMARTer® RACE 5’/3’ Kit (Clontech, TaKaRa Bio, USA) as per manufacturer’s instructions followed by Sanger based sequencing of the RACE products ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of extracted RNA was subjected to one-step real-time RT-PCR using a One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.) on a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNAs were subjected to one-step real-time PCR using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.).
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bioRxiv - Immunology 2023Quote: ... RNA isolation and cDNA amplification was performed using either Smart-Seq® v4 Ultra® Low Input RNA Kit (Takara Clontech, cat# 635015) or NORGEN BIOTEK (cat# 51800) ...
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bioRxiv - Immunology 2023Quote: ... RNA isolation and cDNA amplification was performed using either Smart-Seq® v4 Ultra® Low Input RNA Kit (Takara Clontech, cat# 635015) or NORGEN BIOTEK (cat# 51800) ...
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bioRxiv - Molecular Biology 2022Quote: ... The double stranded barcode library was subsequently cloned into the linearized vector using the In-Fusion® HD Cloning Kit (Clontech® Laboratories) following instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... the first-strand cDNA was synthesized from 1 μg total RNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... An RNA amount equivalent to 12 ng was used to prepare the sequencing library using SMARTer® smRNA-Seq Kit for Illumina® (Takara 635031) according to the kit manual till cDNA synthesis ...
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bioRxiv - Neuroscience 2023Quote: ... then cDNA was synthesized using the SMART-Seq® v4 Ultra® Low Input RNA kit (Takara Bio USA Inc. Cat No. 634888). Libraries were prepared using the NexteraTM DNA Flex Library Prep (Illumina Cat No ...
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bioRxiv - Neuroscience 2023Quote: ... Each of the four pools was converted into one Illumina-barcode indexed sequencing library using the ThruPLEX DNA-Seq HV kit (Takara Bio Catalog# R400740), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... was performed by quantitative real-time polymerase chain reaction (PCR) using the AAVpro™ Titration Kit (for Real Time PCR) Ver.2 (Takara Bio Inc), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 1μg of isolated RNA was taken from each sample was then transcribed to cDNA using PrimeScript ™ RT reagent kit with gDNA Eraser (Takara Bio RR047B). According to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The first-strand cDNAs of mRNA and mature miRNA were synthesized from 500 ng of total RNA using PrimeScript™ RT reagent kit with gDNA Eraser (Cat# RR047A, Takara, Kyoto, Japan) and miRcute Plus miRNA First-Strand cDNA Synthesis Kit (Cat# KR211 ...
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bioRxiv - Biochemistry 2023Quote: ... The synthetic genes were cloned into the NdeI/XhoI site of the pET-28a(+) expression vector by In-Fusion cloning using the In-Fusion HD Cloning Kit (Takara Bio, Kusatsu, Japan), yielding expression constructs with an N-terminal hexahistidine tag and thrombin tag ...
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bioRxiv - Plant Biology 2023Quote: The 5’ and 3’ experiments were conducted with the use of SMARTer® RACE cDNA Amplification Kit (Takara Bio, United States, Cat# 634860) and Advantage Polymerase as described by Kruszka et al ...
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bioRxiv - Cell Biology 2023Quote: ... Final cDNA Libraries were prepared from twice immunoprecipitated RNA and fragmented input total RNA using the Smarter® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio 634411) and sequenced on a Novaseq 6000 ...
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bioRxiv - Plant Biology 2023Quote: ... RNA extraction and genomic DNA removal were performed using a Total RNA Extraction Kit (Plant) (RBC Bioscience, New Taipei, Taiwan) and DNase I (Takara Bio, Shiga, Japan) following the manufacturers’ protocol ...
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bioRxiv - Bioengineering 2023Quote: ... CCR5-KO donor rAAV6 virus was produced in HEK-293T cells and was purified with AAVpro Purification Kit (TakaRa, San Jose, CA, USA). Electroporation of the RNP complex was performed using the Lonza 4D-Nucleofector (Lonza Group Ltd ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA from a microgram of RNA was obtained using a PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan) following its instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from the above total extracted RNA as a template using a PrimeScript First-Strand cDNA Synthesis Kit (Takara Bio, Beijing, China). Specific primers (Table 1 ...
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bioRxiv - Bioengineering 2023Quote: ... T-2A-H2B-tdTomato was generated by replacing the EGFP fragment with H2B-tdTomato using the In-Fusion® HD Cloning Kit (Catalog #639648, Takara, Kusatsu, Japan), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... One microgram of total RNA was used for complementary DNA (cDNA) synthesis by PrimeScript first strand cDNA synthesis kit (TAKARA-Bio, Cat# 6110) using an oligo(dT ...
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bioRxiv - Microbiology 2023Quote: ... The DNA free RNA was used for cDNA preparation using Takara first strand cDNA synthesis kit (cat no.6110A, Takara Bio Inc., Japan) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... obtained by reverse-transcribing the total RNA extracted from HCT116 cells using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Dalian, China) was used as the template for amplifying the corresponding regions using the PrimeSTAR Max DNA Polymerase (Takara Bio) ...
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bioRxiv - Cell Biology 2023Quote: ... The synthesized sequences were ligated to an expression construct encoding a truncated form of OsPHR (Δ1-51OsPHR) using restriction enzyme cloning or InFusion cloning (InFusion HD Cloning kit, Takara Bio, Shiga, Japan). The modified PHR was cloned into the binary vector pMpGWB106 (Ishizaki et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... high-quality cDNA was generated from the total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio, Cat. #634889), and RNA-seq libraries were generated using the SMARTer ThruPLEX DNA-Seq Kit (Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: ... 3µg of DNA was digested by EcoRI and the digested DNA was self-ligated using DNA Ligation Kit (Mighty Mix; Takara Bio, Kusatsu, Japan) after purification by ethanol precipitation ...
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bioRxiv - Neuroscience 2024Quote: Library preparation and RNA sequencing were performed by Novogene Co using the SMART-Seq v4 PLUS Kit (Takara Bio, Kusatsu, Shiga, Japan, # R400752). Total RNA was reverse transcribed into the first-strand cDNA ...
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bioRxiv - Neuroscience 2024Quote: ... PCR amplification of the fragments and their subsequent ligation was performed using the In-Fusion cloning kit and online tools (BD Clontech, Takara Bio, USA), or they were synthesized by GenScript (Piscataway ...
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bioRxiv - Cell Biology 2024Quote: A total of 250 pg to 10 ng RNA per sample was used as input material for sequencing libraries (1–500 ng for small RNA libraries) using the SMARTer Stranded Total RNA-Seq Kit V2 (Takara Bio USA, Inc.) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... Reverse-transcription and amplification were performed using the One Step PrimeScript™ RT-PCR Kit (RR064A, Takara Bio Inc., Kusatsu, JP-25, Japan) with the following forward primer (5’-ATGAGYCTTYTAACCGAGGTCGAAACG-3’ ...
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bioRxiv - Genetics 2024Quote: 1 µg of gDNA Eraser-treated RNA was reverse transcribed into cDNA using the PrimeScrip® RT reagent Kit with gDNA Eraser (Takara Bio, Japan), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 20 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio USA. Inc), which converts poly(A ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was reverse transcribed from 1 μg of total RNA using the PrimeScriptTM RT reagent kit (AK4201; Takara Bio, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Extracted RNA samples and DNA standards were then amplified using One Step PrimeScript™ RT-PCR Kit (TAKARA BIO INC, Kusatsu, Shiga, Japan) and the following primer set ((Forward ...
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bioRxiv - Microbiology 2024Quote: ... was used to determine 5’ and 3’ terminal nucleotide sequences of PcAEV4 and PcAEV5 with the SMARTer® RACE 5’/3’ Kit (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: The coding sequence of BGI1 was inserted into the pGEX-4T-1 vector using the In-Fusion HD cloning kit (Takara Biotech, Shiga, Japan). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... the miR-708-5p.T from CAG-eYFP-3x-miR708-5p-TS was amplified by PCR and inserted into WPRE-ab at the KpnI restriction enzyme site using the In- Fusion Cloning Kit (TaKaRa Bio, Shiga, Japan). To construct the plasmids ab-WPRE or ab- WPRE-ab (pAAV/mIba1.GFP.miR-9.T.miR-129-2-3p.T.WPRE.SV40pA or pAAV/mIba1.GFP.miR-9.T.miR-129-2-3p.T.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA
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bioRxiv - Physiology 2024Quote: ... was synthesized with 1 μg of total RNA for each sample using the PrimeScript™ RT reagent Kit with gDNA Erase (Takara Bio Inc.) according to the manufacturer’s instructions and stored at -20 ℃ until use.
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bioRxiv - Microbiology 2024Quote: ... Single-cell whole transcriptome amplifications were carried out using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech, cat. 634895, 634896) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... These plasmids were generated by partial deletion of the pP1-8 plasmid using specific primer sets and PrimeSTAR Mutagenesis Basal kit (Takara Bio, Shiga, Japan). Recombinant P1 peptides were produced in E ...
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bioRxiv - Plant Biology 2024Quote: ... The PCR-amplified product was cloned into the HindIII sites of pMpGWB301 (Ishizaki et al., 2015) using the In-Fusion HD cloning kit (Clontech, Mountain View, CA). The CRISPR-Cas9-resistant MpRLF coding sequence in pENTR-MpRLFcds_resistant was introduced into the binary vector using the Gateway LR clonase II Enzyme mix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... 8 ul of cDNA 0.125 ng/μL was used as a starting material for library preparation using the the Unique Dual Index (UDI) kits (Takara bio, 634752-5) according to the vendor protocol ...
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bioRxiv - Biochemistry 2024Quote: ... PDIA6 domains (a0, a, b) and mutants (a0-A5, b-ND) were constructed using a PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Shiga, Japan). All PDIs used in this study were overexpressed in the Escherichia coli strain BL21 (DE3 ...
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bioRxiv - Immunology 2024Quote: ... 200ng RNA from antigen induced or unstimulated PBMCs was subjected for preparing TCR cDNA libraries by using the SMARTer Human TCR a/b Profiling Kit (Takara Bio USA, Inc). The kit utilizes SMART technology (Switching Mechanism At 5’end of RNA Template ...
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bioRxiv - Bioengineering 2024Quote: ... AphAI was amplified using primers 9 and 10 listed in Supplementary Table 1 from pEPkan-S and inserted into the PstI site of pmRFP using the In-Fusion® HD Cloning Kit (Takara Bio USA).
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was extracted from the yeast cells grown at 25°C in YPD medium and mRNA was purified using the Oligotex TM-dT30 mRNA Purification Kit (Takara Bio Inc., Japan). Biotinylated cRNA was prepared from 500 ng of mRNA according to the standard Affymetrix protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Two micrograms of RNA from each sample was used to synthesize cDNA using PrimeScript RT reagent kit (Takara Biotechnology Co. Ltd. Dalin, China) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: Full-length double-strand cDNA for each oocyte or zygote was prepared using an sc-RNA-seq libraries SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, Mountain View, CA) following the vendor’s instruction ...