Labshake search
Citations for Takara Bio :
4951 - 5000 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... A total of 1 µg of RNA was reverse transcribed to complementary DNA using the PrimeScript™ RT reagent Kit (Takara Bio, Otsu, Japan), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: The cDNA prepared from 10-day-old OsbZIP48OE seedlings was used to perform 5’ and 3’ RACE using SMARTer® RACE 5’/3’ Kit (Clontech, TaKaRa Bio, USA) as per manufacturer’s instructions followed by Sanger based sequencing of the RACE products ...
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IRF1 regulates self-renewal and stress-responsiveness to support hematopoietic stem cell maintenancebioRxiv - Cell Biology 2023Quote: ... cDNA generation and amplification were performed using the SMART-Seq® v4 Ultra® Low Input RNA Kit for sequencing (Takara Bio USA, Inc.). Quality check was performed using High sensitivity D5000 ScreenTape® on TapeStation Analysis Software 3.2 (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 µg of quality checked total RNA was converted into first-strand cDNA using a PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa Bio Inc, Shiga, Japan), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 5′-GCATGTCACGATGTTACAA-3′ and 5′-GGATGAAATGAAGGTGCTA-3′ as previously described50 and were inserted using the Infusion HD Cloning kit (Takara Bio USA Inc, NC1470242).
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bioRxiv - Bioengineering 2023Quote: ... The synthesized gene fragments were then cloned into the linear pET21a vector using the In-Fusion® HD Cloning Kit (Takara Bio, Kusatsu, Japan). For mammalian expression ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA isolation and cDNA synthesis were carried out using the SMARTer® Ultra® Low Input RNA Kit for Sequencing – v4 (Clontech, Cat.no. 634888). Libraries were generated from the resulting cDNA (0.2ng/ul per sample ...
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bioRxiv - Plant Biology 2023Quote: ... One microgram of total RNA was used to synthesize cDNA using Oligo dT primers and Smart MMLV Reverse Transcriptase kit (Takara Bio Inc, Shiga, Japan). The expression of NIa-Pro was verified in all samples using gene specific primers and RT-PCR (Fig S1a ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reactions at every examined depth were performed in triplicate (n=3) using Takara SpeedSTAR HS DNA polymerase kit (Takara Bio USA, Madison, WI) with the following modifications ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted from the injected aphid nymphs 24 h post-injection using the Takara Nucleospin RNA Plus XS RNA extraction kit (Takara Bio Inc., Shiga, JP). For RNA extraction ...
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bioRxiv - Cell Biology 2023Quote: ... The reporter cassette excised from pPExRF3 and the left and right homology arms were assembled and cloned into a linearized pUC19 in a single enzymatic reaction using the In-Fusion Cloning Kit (Clontech Laboratories Inc., California, U.S.A). A gRNA vector was constructed by cloning a 20-bp target sequence surrounding the stop codon of coronin into pU6 (FlyCRISPR) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA input was normalized across samples and RNA libraries were prepared utilized the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara, 634890, 634888) followed by the Nextera XT DNA library preparation kit (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... Ultra-low input RNA sequencing library was prepared by using SMART-Seq HT kit for full-length cDNA synthesis and amplification (Takara, San Jose, CA, USA), and Illumina Nextera XT (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... The amplified linear fragment of pKNOCK-Km and the internal fragments of each gene were fused using In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The rRNA genes (18S, ITS, 28S) were amplified by polymerase chain reaction (PCR) using the TaKaRa Ex Taq polymerase kit (TaKaRa Bio-medicals, Seoul, Korea). PCR cycling parameters and primer information were followed as in Shazib et al ...
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bioRxiv - Evolutionary Biology 2024Quote: ... WTAs of the individual ciliates were generated according to the Smart-seq2 protocol (Picelli et al. 2014) and SMART-Seq v4 Ultra Low Input RNA Kit (TaKaRa Bio-medicals, Seoul, Korea) using 17 cycles of cDNA amplification for each cell ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR product was cloned into the KpnI site of the pCRtk2×2NN vector (RDB18670) to generate pCRtk-PDX1 using an In-Fusion HD Cloning Kit (639648; Takara Bio Inc., Shiga, Japan). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... hdhfr and ef-1α 3’NR were cloned into the BamHI site of pBluescript SK using In-Fusion HD Cloning Kit (Takara Bio Inc., Otsu, Japan). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... RNA sequencing library was prepared using the SMARTer Stranded RNA Total RNA Sample Prep Kit - Pico Input (Takara Bio USA, San Jose, CA, USA), as previously described68 ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA from the same amount of RNA between RNAi and control samples were synthesized in a 20 μl reaction volume by the PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA, Code No. RR047A). One μl of cDNA was used as the template in a 10 μl reaction volume of the PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... and the BsAUX/IAA16 amplicons were ligated into the digested vector using the In-Fusion Cloning kit (Takara Bio USA, Inc., San Jose, CA). Plasmid sequencing ensured correct assembly of expression vectors (Plasmidsaurus ...
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bioRxiv - Neuroscience 2024Quote: ... The viral titer (p24 core antigen content) was determined initially using a LentiX GoStix Plus titration kit (#631280, Takara Bio, San Jose, CA, USA) and subsequently using an HIV p24 AlphaLISA detection kit (#AL291C ...
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bioRxiv - Microbiology 2024Quote: ... the ORF4 region was amplified by RT-PCR with specific primers (Supplementary Table S2) using PrimeScript One Step RT-PCR Kit Ver.2 (Takara Bio Inc., Kusatsu, Japan), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... A library was independently prepared with 10 ng of total RNA for each sample by SMARTer ultra-low input RNA Sample Prep Kit (Clontech Laboratories, Inc., CA, USA). The first step in the workflow involves purifying the poly-A containing mRNA molecules using poly-T-attached magnetic beads ...
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bioRxiv - Cell Biology 2024Quote: Cell content was used as direct input for full length cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio, San Jose, CA) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Ten nanograms of RNA were used for preparing indexed libraries using SMARTer Stranded Total RNA-Seq Pico-Input Mammalian kit v.3 (Takara Bio. Cat# SKU: 634487) using manufacturer’s instructions with a couple of modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... Then the cell lysates were used for reverse transcription and 10-cycle amplification by SMART-Seq HT kit seq (Takara Bio USA, Cat. 634437) following the user manual ...
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bioRxiv - Immunology 2022Quote: ... 0.5 µg of the isolated RNA was reverse transcribed to cDNA using an oligo dT primer and 12.5 units of reverse transcriptase mix (PrimeScript RT Reagent Kit, TaKaRa Bio, Saint-Germain-en-Laye, France). cDNA corresponding to 10 ng total RNA served as the template in a real-time PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... was amplified by RT-PCR and cloned into pMSCV-IRES-eGFP using In-Fusion® HD Cloning Kit User Manual (Takara, Cat#638920, Shiga, Japan) following the manufacturer’s instructions (see Table 3 for primer sequences).
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bioRxiv - Microbiology 2020Quote: ... was reverse-transcribed to cDNA using random hexamers and oligo (dT) by following the instructions provided in the Prime-Script RT Reagent Kit (Perfect Real Time from TaKaRa Bio Inc., Otsu Shiga, Japan). RT-qPCR was carried out in a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Systems Biology 2020Quote: ... RNA was eluted in 10 µl water and immediately proceeded to cDNA generation using the SMART-Seq V4 Ultra Low RNA Kit (Takara Bio USA, Mountain View, CA). 1 ng of the resulting cDNA from 14 PCR cycles was “tagmented” and barcoded by using a Nextera XT DNA Sample Preparation Kit (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... recombinant sites including the 900 base pair upstream/downstream flanking region and the 100 base pair coding sequence of the target genes were cloned into pK18-CmR-pheS** plasmid backbone by using an In-Fusion® HD Cloning Kit (TAKARA Bio; Kusatsu, Shiga, Japan) to generate the plasmid (aedF- ...
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bioRxiv - Zoology 2021Quote: ... The reverse transcription of 1 μg of total RNA was performed using PrimeScript® RT reagent kit with gDNA Eraser (RR047A, Takara Bio Inc., Dalian, China). The gene expression was determined through RT-PCR ...
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bioRxiv - Biochemistry 2022Quote: ... one microgram of total RNA was employed to obtain the corresponding cDNA target sequences using an oligo(dT)18 primer and the PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan), following the manufacturer’s directions ...
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bioRxiv - Microbiology 2021Quote: Antibody sequencing was performed as previously described (Simonich et al., 2019; Vigdorovich et al., 2016) using the SMARTer RACE 5’/3’ Kit (Takara Bio USA Inc., Mountainview, CA). Briefly ...
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bioRxiv - Evolutionary Biology 2022Quote: ... library was prepared according to the Isoform Sequencing protocol (Iso-Seq) using the Clontech SMARTer® PCR cDNA Synthesis Kit (Clontech Laboratories (now Takara Laboratories), 634926 ...
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bioRxiv - Neuroscience 2021Quote: ... Flash frozen cell samples were sent to the Cambridge Genomic Services for library preparation using SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio USA, Inc), followed by RNA sequencing (NextSeq 74 cycle high output 15-20 M reads per sample ...
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bioRxiv - Physiology 2020Quote: ... The PCR products were subsequently cloned into an EcoRI-HF® (New England BioLab, MA, USA) linearized pIRESZ2_ZsGreen1 vector using In-Fusion® HD cloning kit (TaKaRa Bio Inc, Kusatsu, JP). After sequence validation (Eurofin ...
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bioRxiv - Genetics 2020Quote: ... nucleotides 610–792 of the map3 ORF were subjected to random mutagenesis by using the Diversify PCR Random Mutagenesis Kit (Clontech Laboratories, Mountain View, CA, USA) in accordance with the manufacturer’s instructions for error-prone PCR ...
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bioRxiv - Plant Biology 2021Quote: ... and the library preparation was made with the SMART-Seq™ v4 Ultra™ Low Input RNA Kit for Sequencing (Takara, Mountain View, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNAs that were used for RACE were synthesised from 1 µg of polyA+ RNA using a SMART RACE cDNA Amplification Kit (Clontech Laboratories, Inc., Palo Alto, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from 1 ng RNA using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio Europe SAS). Libraries were prepared from the purified cDNA using the Nextera™ XT DNA Library Preparation Kit (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... The amplified 0.5 kbp fragment was inserted into the BamHI site of pTK2051 using the In-Fusion Snap Assembly kit (Takara Bio USA, Mountain View, CA, USA). The resulting suicide plasmid was introduced into B ...
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bioRxiv - Systems Biology 2022Quote: ... The PCR products were subsequently cloned into pGEMHE Xenopus laevis oocyte expression vector using In-Fusion® HD cloning kit (TaKaRa Bio Inc, Kusatsu, JP) and the final sequence validated (Eurofin ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were amplified and synthesized into cDNA according to the manufacturer’s protocol using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio Inc., Shiga, Japan) and amplification yield was checked using a Bioanalyzer (Agilent High Sensitivity DNA Kit ...
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bioRxiv - Microbiology 2022Quote: ... The amplified fragments were then fused with SmaI-linearized pUt-C-EGFP10 using the In-Fusion HD Cloning Kit (Clontech Laboratories, Mountain View, CA, USA). Gene sequences fused with the egfp gene were positioned in tandem between the linker sequences ...
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bioRxiv - Microbiology 2022Quote: ... DNAse treated-RNA was reverse transcribed into cDNA using random hexamers and oligo (dT) primers with the Prime-Script RT Reagent Kit (Perfect Real Time from Takara Bio Inc., Otsu Shiga, Japan). Viral presence was assessed through RT-qPCR (StepOnePlus Real-Time ...
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bioRxiv - Immunology 2024Quote: ... The full-length CD177 cDNA inserts were sub-cloned into pBMN-I-EGFP retroviral vector digested with Bam HI and EcoR I restriction enzymes using InFusion HD Cloning Kit (Takara Bio USA, Mountain View, Ca) according to the product manual ...
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bioRxiv - Cancer Biology 2024Quote: We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC-97L cells using the SMARTer™ RACE cDNA Amplification Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... the library was prepared by synthesizing cDNA via reverse transcription and amplifying cDNA by long-distance PCR using the Takara SMARTer v4 kit (Takara Bio USA, San Jose, CA). 2×150bp sequencing was performed on an Illumina NovaSeq6000 instrument ...