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Citations for Takara Bio :
4801 - 4850 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and Foxa3 were obtained by reverse transcription PCR using a template RNA extracted from a C57BL/6J liver using a PrimeScript™ II 1st strand cDNA Synthesis Kit (TAKARA Bio, Japan). The coding sequences of the primers used are listed in Table S3 ...
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bioRxiv - Physiology 2020Quote: ... containing 1 μL of 10× reaction buffer (SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing, Cat. No. 634888, Takara Bio, Kusatsu, Japan). Nuclease-free water was added to 11.5 μL ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently used as a template to generate all other HaloTag constructs using the In-Fusion HD Cloning Kit (Takara Bio USA, Inc), with the exception of pReciever-M49(HaloTag-SOX17 ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by RNA isolation according to the manufacturer’s instructions (SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing, Takara Bio USA) (Figure 2A ...
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bioRxiv - Molecular Biology 2021Quote: ... One µg of total RNAs was subjected to reverse transcription into cDNAs using PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara, cat. no. RR047A) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... four sequencing libraries (one for each tissue type) were constructed using the SMARTer PCR cDNA Synthesis Kit (ClonTech, Takara Bio Inc., Shiga, Japan) by tissue type with no size selection by NovoGene ...
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bioRxiv - Plant Biology 2020Quote: ... four sequencing libraries (one for each tissue type) were constructed using the SMARTer PCR cDNA Synthesis Kit (ClonTech, Takara Bio Inc., Shiga, Japan) by tissue type with no size selection by NovoGene ...
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bioRxiv - Microbiology 2021Quote: RsmY and RsmZ expression quantification was done by RT-PCR using Mir-X miRNA qRT-PCR TB Green Kit (Takara Bio USA, Inc). For each reaction ...
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bioRxiv - Plant Biology 2021Quote: Bait constructs for Y1H analysis were produced by inserting promoter regions of SDI genes into the multiple cloning site of pTUY1H using In-Fusion® HD cloning kit (Takara Bio Inc.) and then used to transform yeast strain Y187 ...
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bioRxiv - Developmental Biology 2021Quote: ... and the total RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa, RR047A, Kusatsu, Shiga, Japan). The diluted cDNA was used as a template for qPCR ...
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bioRxiv - Microbiology 2021Quote: ... The extracellular SARS-CoV-2 RNA in the culture supernatant was analyzed using SARS-CoV-2 direct detection RT-qPCR kit (RC300A; TaKaRa-Bio, Shiga, Japan). The infectivity of SARS-CoV-2 in the culture supernatants was determined at 48 h post-infection ...
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bioRxiv - Genetics 2020Quote: ... RNA was extracted from samples using TRIzol and cDNA synthesized from 1 μg RNA template using PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA BIO INC). The sequences of primers used for amplification are shown in Table S6 ...
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bioRxiv - Biochemistry 2021Quote: ... In-fusion cloning was used for the ligation of sequence fragments to vector with the In-fusion HD Cloning Kit (Clontech Laboratories, Inc, US). Site-directed mutations were accomplished according to the protocol provided in a Mut Express II Fast Mutagenesis kit V2 (Vazyme ...
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bioRxiv - Biochemistry 2021Quote: ... vector (including the N-terminal hexahistidine and thrombin tags) by In-Fusion cloning using the In-Fusion HD Cloning Kit (Takara Bio, Kusatsu, Japan), yielding the pBAD-yTrm5 vector ...
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bioRxiv - Biophysics 2020Quote: ... tinctorius Nav1.4 were determined by DNA gel extraction and sequencing after rapid amplification of cDNA ends (RACE) using the SMARTer® RACE 5’/3’ Kit (Takara Bio, USA) and internal primers designed from P ...
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bioRxiv - Cell Biology 2021Quote: ... we linearized the pnCS plasmid with NdeI/NheI and employed seamless cloning (In-Fusion HD Cloning Plus Kit from Takara Bio, Cat. # 638910) with the following primers ...
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bioRxiv - Cancer Biology 2020Quote: ... and bound to strand-specific adaptors following the manufacturer’s protocol (SMARTer Stranded Total RNA Sample Prep Kit, Takara Bio, Mountain View, CA, USA). cDNA libraries were assayed for quality control by Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... PCR amplification using proofreading DNA polymerases (Phusion HF, New England Biolab, Ipswich, MA) and In-Fusion HD Cloning Kit (Clontech, Mountain View, CA) or NEBuilder HiFi DNA Assembly Cloning Kits (New England Biolab) ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription was then carried out using total RNA and oligo-dT primers to synthesize the first strand cDNA using the PrimeScript One-Step RT-PCR Kit (Ver.2, Takara Biotechnology, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA (1 μg) was reverse transcribed to cDNA using the PrimeScriptTM RT reagent kit with gDNA Eraser (Cat#: RR047A, TaKaRa Bio, Shiga, Japan). qRT–PCR was performed using a LightCycler 480 II instrument (Roche Molecular Systems ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from total RNA of each culture sample using the PrimeScript II 1st strand cDNA Synthesis Kit (Takara Bio, Shiga, Japan) with random hexamers to yield a total volume of 20 μL ...
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bioRxiv - Immunology 2020Quote: ... the 5×PrimeSTAR® Buffer (Mg2+ plus) in the kit was replaced with the 2 × PrimeSTAR® GC Buffer (Mg2+ plus) (Takara, Japan). The first PCR program was as follows ...
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bioRxiv - Immunology 2021Quote: ... T cell receptor sequencing libraries were prepared with the SMARTer Human TCR α/β Profiling Kit (catalog number 635015, Takara Bio USA, Inc.) according to manufacturer’s instructions with the exception of excluding the third and fourth bead size selection steps listed in Table 3 of the kit manual ...
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bioRxiv - Microbiology 2021Quote: ... for 15 min and then harvested for quantification of INF α mRNA using the One-step SYBR Prime script RT-PCR kit (TaKaRa, Dalian, China) according to the manufacturer’s protocol.Briefly the total RNA was isolated from TNF-α-treated with Trizol reagent (Takara Bio ...
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bioRxiv - Plant Biology 2023Quote: The cDNA prepared from 10-day-old OsbZIP48OE seedlings was used to perform 5’ and 3’ RACE using SMARTer® RACE 5’/3’ Kit (Clontech, TaKaRa Bio, USA) as per manufacturer’s instructions followed by Sanger based sequencing of the RACE products ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of extracted RNA was subjected to one-step real-time RT-PCR using a One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.) on a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNAs were subjected to one-step real-time PCR using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.).
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bioRxiv - Immunology 2023Quote: ... RNA isolation and cDNA amplification was performed using either Smart-Seq® v4 Ultra® Low Input RNA Kit (Takara Clontech, cat# 635015) or NORGEN BIOTEK (cat# 51800) ...
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bioRxiv - Immunology 2023Quote: ... RNA isolation and cDNA amplification was performed using either Smart-Seq® v4 Ultra® Low Input RNA Kit (Takara Clontech, cat# 635015) or NORGEN BIOTEK (cat# 51800) ...
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bioRxiv - Molecular Biology 2022Quote: ... The double stranded barcode library was subsequently cloned into the linearized vector using the In-Fusion® HD Cloning Kit (Clontech® Laboratories) following instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... the first-strand cDNA was synthesized from 1 μg total RNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: RNA was reversely transcribed into 1st strand cDNA in a 20 μL reaction volume using the PrimeSeriptTM1stStrand cDNA Synthesis Kit (TaKaRa Bio, Dalian, China) following the manufacturer’s protocol of Reverse Transcription System ...
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bioRxiv - Microbiology 2023Quote: ... and real-time RT-PCR was performed by targeting the SARS-CoV-2 RdRp gene as follows: each 20 µl sample consisted of 12.5 µl One Step PrimeScript™ III RT-PCR Kit (Takara Bio, Shiga, Japan), 0.5 µM Sars-CoV-2 CRV forward primer (5’-TCACCTAATTTAGCATGGCCTCT-3’) ...
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bioRxiv - Microbiology 2023Quote: ... or the BamHI/MluI site of pWPI-ACE2-zeo (for ACE2 expression plasmids)43 with 3×FLAG-tag at the C-terminus using In-Fusion® HD Cloning Kit (Takara, Cat# Z9650N). Nucleotide sequences were determined by DNA sequencing services (Eurofins) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The isolation of the upstream genome sequences of gnrh1 and gnrh3 of piranha was performed using the Universal GenomeWalker ™ kit (Takara Bio USA) according to the instructions ...
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bioRxiv - Bioengineering 2023Quote: ... CCR5-KO donor rAAV6 virus was produced in HEK-293T cells and was purified with AAVpro Purification Kit (TakaRa, San Jose, CA, USA). Electroporation of the RNP complex was performed using the Lonza 4D-Nucleofector (Lonza Group Ltd ...
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bioRxiv - Neuroscience 2023Quote: ... then cDNA was synthesized using the SMART-Seq® v4 Ultra® Low Input RNA kit (Takara Bio USA Inc. Cat No. 634888). Libraries were prepared using the NexteraTM DNA Flex Library Prep (Illumina Cat No ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The first-strand cDNAs of mRNA and mature miRNA were synthesized from 500 ng of total RNA using PrimeScript™ RT reagent kit with gDNA Eraser (Cat# RR047A, Takara, Kyoto, Japan) and miRcute Plus miRNA First-Strand cDNA Synthesis Kit (Cat# KR211 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1μg of isolated RNA was taken from each sample was then transcribed to cDNA using PrimeScript ™ RT reagent kit with gDNA Eraser (Takara Bio RR047B). According to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Each of the four pools was converted into one Illumina-barcode indexed sequencing library using the ThruPLEX DNA-Seq HV kit (Takara Bio Catalog# R400740), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... was performed by quantitative real-time polymerase chain reaction (PCR) using the AAVpro™ Titration Kit (for Real Time PCR) Ver.2 (Takara Bio Inc), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... An RNA amount equivalent to 12 ng was used to prepare the sequencing library using SMARTer® smRNA-Seq Kit for Illumina® (Takara 635031) according to the kit manual till cDNA synthesis ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified PCR products and pJM101/L1.3FLAG plasmid DNA then were digested with NotI and AgeI and ligated using the DNA Ligation Kit Mighty Mix (TaKaRa Bio, Shiga, Japan) at 16°C for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: 50 ng “treated” RNA sample and 10 ng “untreated” RNA sample were subjected to library construction using SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara Bio, 634485) according to the manufacturer’s protocol with several modifications ...
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bioRxiv - Microbiology 2022Quote: ... the lentiviral transfer plasmid pCSII-EF-luciferase (Agarwal et al., 2006), and pCMV-VSV-G (Stewart et al., 2003) using the CalPhos Mammalian Transfection Kit (Takara Bio Company, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... the Aprt cDNA was subcloned into the pUASTattB vector (Bischof et al. 2007) using the In-Fusion HD Cloning Kit (Takara Bio, Kyoto, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... total RNA was isolated by standard procedure from 20-30 heads of homozygous Aprt5 flies and reverse transcribed using oligo d(T) primers (PrimeScript RT Reagent Kit, Takara Bio, Kyoto, Japan). At least 750 ng of the first strand cDNA was amplified by PCR in 20 μl of reaction mixture using PrimeStar Max DNA polymerase (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: ... Equal amounts of total RNA were used to synthesize cDNA with the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara RR047A, China). Quantitative real-time reverse-transcription PCR (qRT–PCR ...
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bioRxiv - Physiology 2024Quote: ... Each half-site of the responsible ERE-like sequence was mutated into a HindIII recognition site using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Shiga, Japan). Transcriptional activity assays with these constructs were done as described above ...
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bioRxiv - Genetics 2024Quote: Two μg of total RNA was reverse transcribed into cDNA templates using RNA to cDNA EcoDry™ Premix kit including both random hexamer and oligo(dT)18 primers (Takara Bio, 639548). KAPA SYBR® FAST qPCR Master Mix (2X ...