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Citations for Takara Bio :
4501 - 4550 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The first-strand cDNA templates were generated by reverse transcription using a double-primed RNA to cDNA EcoDry™ Premix kit (TaKaRa, Clontech ...
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bioRxiv - Neuroscience 2024Quote: ... RNA concentration was assessed by Nanodrop and RNA-seq libraries were prepared from 10 ng of total RNA using the SMARTer Stranded Total RNA-seq Pico Input Mammalian V2 kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The titer of the purified virus stocks was determined in viral genomes (vg)/ml using an AAV titration kit (Takara Bio).
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bioRxiv - Cancer Biology 2024Quote: ... reverse primer 5-TACCAAACTCTCAATTGCTC-3’) and the presence of small insertions and deletions assessed with the Guide-it Mutation Detection Kit (Takara Bio). The PCR amplicons were then inserted into pGEM vectors using the pGEM-T Easy Vector system (Promega ...
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bioRxiv - Genetics 2024Quote: ... Immunoprecipitated RNA was subjected to DNA library preparation using SMARTer Stranded Total RNA-Seq Kit V3—Pico Input Mammalian (Takara Bio) according to the manufacturer’s instructions with initial fragmentation at 94°C for 4 min and including the ribosomal RNA depletion step ...
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bioRxiv - Biochemistry 2024Quote: A pLK35 plasmid with an MS2 tagged located in 16S rRNA helix h6 was a gift from the Schepartz lab at UC Berkeley.19 The helix h26 extensions were introduced using the In-Fusion Cloning Kit (Takara Bio) with the primers listed below ...
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bioRxiv - Cancer Biology 2024Quote: ... The ChIP-seq library was prepared according to the manufacturer’s instructions using the Thruplex DNA-seq Kit (R400675, Takara Bio, Japan). The prepared ChIP-seq libraries were sequenced by Macrogen ...
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bioRxiv - Bioengineering 2024Quote: ... Transfected HEK293T cells were cultured for 3 more days and then AAV was collected using the AAVpro Purification Kit Midi (Takara, 6675) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: Flash-frozen cerebral cortical samples were homogenized by bead-beating for 30 seconds with RNAse-free stainless-steel beads (Midsci SSB14B-RNA) in 300 µL of lysis buffer provided by the NucleoSpin 96 RNA isolation and purification kit (Takara 740709.4). Lysates were centrifuged at 13,000 x g for 5 minutes to remove beads and debris ...
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bioRxiv - Bioengineering 2024Quote: 0.1 mg of total RNAs were served as template for reverse transcription by using PrimeScript™ II 1st Strand cDNA Synthesis Kit (TAKARA) according to manufacturer’s protocols ...
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bioRxiv - Bioengineering 2024Quote: ... the amplified DNA fragments from the pooled 48 samples were purified by gel extraction using the NucleoSpin Gel and PCR Clean-up Midi kit (TaKaRa, 740986.20). We repeated this DNA purification process every 48 samples ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 μl of reaction master mix consisting of the components of the LDH reagent kit (Takara Bio Inc., Shiga, Otsu, Japan) (reagent A and reagent B in a ratio of 1:45 ...
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bioRxiv - Cell Biology 2024Quote: Bulk-RNA sequencing library is constructed based on Smart-seq2.82 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara catalog #634890) was used and experiments were performed according to manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... assembled with the ICP0-HindIII fragment digested from pICP0-HindIII using the In-Fusion® HD Cloning Kit (Takara Bio USA), and cloned into pVB-ICP0-MCS-GFP ...
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bioRxiv - Biochemistry 2024Quote: ... were obtained by gene synthesis (IDT) and cloned into pOPIN-S vector 36 using the In-Fusion HD Cloning Kit (Takara Clontech). SnJOS2 was amplified from S ...
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bioRxiv - Cell Biology 2024Quote: Adenoviruses containing a full-length FHOD3L wild-type construct were generated by transiently transfecting HEK293 cells using the CalPhos Mammalian Transfection Kit (Catalog No. 631312, Takara Bio) with pAdenoX-CMV-3xHA-FHOD3L-CMV-DsRed after digesting with PacI (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared from 600-1000 pg of total RNA using the SMART-Seq v4 Ultra Low Input Kit (Takara Bio) followed by Nextera XT DNA Library Preparation kit (Illumina) ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid DNA used for transfection was prepared using either the FastGene Plasmid Mini Kit (Nippon Genetics, Japan) or NucleoBond Xtra Midi/Maxi (TAKARA, Japan).
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bioRxiv - Genetics 2024Quote: ... Total RNA was added to reaction buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, #634891), and reverse transcription was performed followed by PCR amplification to generate full length amplified cDNA ...
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bioRxiv - Genetics 2024Quote: RNA amounts were normalized and 500-100ng of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian (Takara Bio). Sequencing was performed on the NextSeq2000 platform (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... 10 ng of total RNA was used to construct a strand- specific RNAseq library with SMARTer Stranded Total RNA-Seq Kit - Pico Input (Takara, 634487). The quality and the size of the libraries were analysed with TapeStation System according to the assay guide ...
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bioRxiv - Developmental Biology 2024Quote: ... Paired-end library preparation for samples containing <500 ng of RNA was generated by the core using the SMARTer Stranded Total RNA-seq kit for low input Ribo-depletion library preparation (Takara 634411). All RNA-seq libraries were run on an Illumina NovaSEQ 6000 or NovaSEQ X aiming for 40 million read pairs/80 million total reads per sample ...
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bioRxiv - Developmental Biology 2024Quote: ... A partial length Mlβ-cat coding sequence was amplified from cDNA using the Advantage RT for PCR kit (Clontech, cat #639506) using the following primers:
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bioRxiv - Plant Biology 2020Quote: ... After 1 μg of total RNA from leaves was reverse-transcribed using a High Fidelity PrimeScript™ RT-PCR Kit (Takara, Japan), and Real-time qRT-PCR was performed using a Thermal Cycler Dice® Real Time System III T950 (Takara ...
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bioRxiv - Plant Biology 2020Quote: One µg of total RNA was used to generate cDNA with the SMARTer PCR cDNA Synthesis kit (Clontech, Mountain View, CA, USA). Fifty µl of cDNA were amplified by 13 PCR cycles with the Kapa HiFi PCR kit (Kapa Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Library generation was performed using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (cat no. 634412, Takara Bio Inc), then submitted to the MIT BioMicro Center for quality control (fragment analysis and qPCR) ...
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bioRxiv - Cancer Biology 2021Quote: ... and the oligonucleotides were inserted into C terminal of ARL4C-EGFP or ARL4C-FLAG-HA using In-Fusion HD Cloning Kit (Takara Bio Inc.).
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bioRxiv - Cell Biology 2020Quote: ... Point mutants were generated by site-directed mutagenesis using the In-Fusion HD cloning kit (TakaraBio/Clontech, Saint-Germain-en-Laye, France). Sequence correctness was verified by sequencing (LGC Genomics ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized and amplified directly from intact cells using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... The above four fragments plus the fragment of the gentamycin resistance cassette flanked by loxp sites were connected and inserted into the linearized plasmid pUC19 using the In-Fusion Cloning Kit (TaKaRa, Daliang, China), generating the plasmid pUC-GSP ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was combined with fragment 2 and the gentamycin resistance cassette flanked by loxp sites and then inserted into the linearized plasmid pUC19 by an In-Fusion Cloning Kit (TaKaRa, Daliang, China) to generate the plasmid pUC19-Gent ...
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bioRxiv - Molecular Biology 2020Quote: ... An amount of 400 ng of the extracted RNA was subjected to reverse transcription by using a PrimeScript RT Reagent Kit (Takara, Shiga, Japan). With complementary DNA (cDNA ...
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bioRxiv - Microbiology 2020Quote: ... 250 ng of RNA isolated from each condition was converted into cDNA and processed through the SMARTer® 5’/3’ RACE Kit (Takara Bio) for 5’-RACE following manufacturer protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... 9 ul from each samples were directly used for cDNA amplification (15 cycles of LD PCR, using the SMART-Seq v4 Ultra Low Input RNA kit for sequencing (Takara Bio, #634898) and the SeqAmp DNA Polymerase (Takara Bio #638509) ...
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bioRxiv - Developmental Biology 2021Quote: ... Pdum-Lox2 and Avi-Post2 the SMART™ (Switching Mechanism At 5’ end of the RNA Transcript) RACE method (SMART™ RACE cDNA amplification kit, Clontech) was used (Brena et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... templates were constructed by cloning the 2 kb 5’ and 3’ homology arms into pPD95.77 plasmids using In-Fusion Advantage PCR cloning kit (Clontech, cat. no. 639621). We used the CRISPR design tool (http://crispr.mit.edu ...
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bioRxiv - Developmental Biology 2020Quote: ... Pegl-17::GFP1-10 constructs were generated by cloning egl-17 promotor and GFP1-10 sequences into pPD95.77 plasmids using In-Fusion Advantage PCR cloning kit (Clontech, cat. no. 639621). The plasmid was injected into SPC-1::GFP11×7 knock-in animals ...
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bioRxiv - Genomics 2020Quote: ... total RNA of midgut samples in treatment groups and control groups were respectively extracted using AxyPrep™ Multisource Total RNA Miniprep Kit (TaKaRa, Japan); (2 ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR products were nested PCR-amplified with primers for IgG VH or IgG VL following the HS DNA polymerase kit protocol (Takara, TAK R010). The IgG VH and IgG VL nested PCR products were purified and next cloned into human IgG1 heavy chain and light chain expression vectors ...
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bioRxiv - Microbiology 2022Quote: ... A maximum of 500 ng of RNA were reverse transcribed with both oligo dT and random primers using a PrimeScript RT Reagent Kit (Perfect Real Time, Takara Bio Inc.) in a 10 µL reaction ...
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bioRxiv - Neuroscience 2021Quote: ... 2 days post-treatment the vehicle control flasks had DNA from 1×108 cells per replicate isolated with Nucleobond CB 500 kit (Takara Bio 740509) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... was amplified using specific primers (forward: 5’-AAGGAGATATACATATGCTCTCCGAAATGGTGGAAGAAG-3’; reverse:5’-GTGCGGCCGCAAGCTTTTATTTCTTTTTGTTGGTGGTCTG-3’) and cloned into pET28a using an InFusion HD Cloning Kit (Takara Bio USA) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing libraries for HEK293T samples were generated using the SMARTer Stranded RNA-Seq Kit which uses a template switching reverse transcriptase (Takara Bio 634837). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified viral RNA (vRNA) was reverse transcribed into cDNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio Inc., Shiga, Japan). The number of RV genome copy in the medium supernatant was determined using RT-qPCR with general or serotype-specific Taqman probe and primers (Additional file 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... were co-transfected into HEK293T cells at a 1:1:1:1.6:4.6 ratio using CalPhos mammalian transfection kit (TaKaRa Clontech, Mountain View, CA #631312) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: The viral loads of WHCV in BALF of patient 1 were determined by quantitative real-time RT-PCR with Takara One Step PrimeScript™ RT-PCR Kit (Takara RR064A) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... the PC1-CTT (mPC-4119) [6] insert was fused with mCherry and cloned into pcDNA3 using an In-Fusion HD Plus kit (Takara Bio, 638909). The expression plasmids for EGFP-SLK (pEGFP-C1+SKL ...
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bioRxiv - Genomics 2021Quote: ... were used for library construction using the SMARTer Stranded Total RNA-Seq Kit v.2 (634418, Pico Input Mammalian, Takara/Clontech, Japan) according to the manufacturer’s protocol without RNA fragmentation ...
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bioRxiv - Cell Biology 2021Quote: ... or mCherry cDNA were cloned at the position next to S252 of DFNA5 cDNA using an In-Fusion HD cloning kit (TaKaRa, Seoul, Korea). All the lentiviral vectors ...
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bioRxiv - Biochemistry 2020Quote: Complementary DNA (cDNA) was generated from previously extracted total RNA (900 ng) using the SMARTER RACE 5’/3’ kit (Takara Bio USA) according to the manufacturer’s instructions ...