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Citations for Takara Bio :
201 - 250 of 2356 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Yeast two-hybrid assay was performed using the Matchmaker Two-Hybrid System (Clontech) by following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Bait and prey constructs were co-transformed into the yeast strain Y2HGold (Clontech) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research ...
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bioRxiv - Genetics 2020Quote: ... following instructions in the Matchmaker® Gold Yeast Two-Hybrid System (Clontech, 630489). Verification of positive colonies was achieved by co-transformation of extracted bait and prey plasmids following the instruction of YeastMaker™ Yeast Transformation System 2 (Clontech ...
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bioRxiv - Molecular Biology 2021Quote: ... following protocol described in the Yeast Protocols Handbook (version no. PR973283 21; Clontech), with both Gal4-BD and Gal4-AD constructs added ...
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bioRxiv - Genetics 2020Quote: Y2H assay was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech) according to the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2022Quote: ... pairwise AD and BD were co-transformed into yeast strain Y2H gold (Clontech). Cells transformed with both plasmids were selected after growth 2 d at 30□ on synthetic dropout medium lacking leucine and tryptophan ...
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bioRxiv - Plant Biology 2022Quote: ... PtoATPE and PtoPSBB were inserted into a pAbAi-BR yeast-integrating vector (Clontech), which was used as a bait-reporter construct ...
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bioRxiv - Plant Biology 2022Quote: All yeast constructs were transformed into the Saccharomyces cerevisiae Y2H Gold strain (Clontech) using Yeastmaker™ Yeast Transformation System 2 (Clontech) ...
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bioRxiv - Plant Biology 2022Quote: The pGBKT7 and pGADT7 plasmids were co-transformed into yeast strain AH109 (Clontech) using the LiCl-PEG (polyethylene glycol ...
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bioRxiv - Genetics 2023Quote: ... Initially the Matchmaker® Gold Yeast One-Hybrid Library Screening System (Takara Bio) was employed ...
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bioRxiv - Plant Biology 2023Quote: ... the CDS of TaNAC016-3A were fused into the yeast vector pGADT7 (Clontech) to generate AD-NAC016-3A prey plasmid ...
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bioRxiv - Systems Biology 2023Quote: ... We modified the Gal4 Matchmaker Gold Yeast Two-hybrid system (clonetech) pGBK_T7 (Clontech) expression vector to harbor the pGADT7_AD vector’s multiple cloning site (MCS ...
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bioRxiv - Plant Biology 2024Quote: ... both provided by Matchmaker™ Gold Yeast Two-Hybrid System (630489, Clontech, USA).
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid assays were performed using the MATCHMAKER GAL4 system (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Growth was determined as described in the Yeast Two-Hybrid System User Manual (Clontech).
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bioRxiv - Plant Biology 2020Quote: ... The amplified fragments were cloned into yeast vectors pGBKT7 and pGADT7 (Clontech, http://www.clontech.com/), which were used to transform yeast strain Y2HGold (Clontech ...
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bioRxiv - Plant Biology 2020Quote: ... All experiments were performed in Y2HGold yeast strain as per the manufacturer’s protocol (Clontech). The plates and liquid cultures were grown in incubator at 30°C with agitation (200 rpm ...
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bioRxiv - Plant Biology 2021Quote: ... Liquid β-galactosidase assays were performed as described in the Yeast Protocols Handbook (Clontech). A Y2HGold yeast strain containing a bait vector was mated with the Y187 strain containing the pGADT7 vector and selected on SD/-Trp/-Leu media ...
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bioRxiv - Plant Biology 2021Quote: pGBKT7-and pGADT7-based constructs were co-transformed into the Y2HGold yeast strain (Clontech) using Yeastmaker™ Yeast Transformation System 2 (Clontech ...
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bioRxiv - Plant Biology 2021Quote: ... The Yeastmaker Yeast Transformation System 2 was used according to the manufacturer’s instructions (Clontech). pGBK-53 and pGADT were used as positive controls ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmids were co-transformed pairwise into AH109 yeast strains (Matchmaker 3 System, Clontech), and selected initially on double drop-out (DDO ...
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bioRxiv - Plant Biology 2023Quote: ... These bait and prey vectors were co-transfected into yeast strain Y2H GOLD (Clontech). The transfected yeast cells were screened on SD/-Leu/-Trp ...
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bioRxiv - Plant Biology 2022Quote: ... pastoris was transformed as described in the yeast protocol handbook (Clontech, Mountain View, USA). Gene replacement and transformation assays for U ...
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bioRxiv - Pathology 2023Quote: Yeast transformation was performed according to the manufacturer’s instructions (Clontech, Mountain View, CA, USA). Yeast cells (strain Y2H Gold ...
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bioRxiv - Plant Biology 2023Quote: Y2H analysis was performed using the Matchmaker® Gold Yeast Two-Hybrid System (Takara) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Pairwise interactions were carried out in the Y2HGold Yeast strain (Cat. no. 630498, Clontech). For transformation of Y2HGold with bait and prey vectors ...
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bioRxiv - Cell Biology 2023Quote: ... Clones in pGADT7 and pGBKT7 were transformed into yeast strain AH109 (Takara Bio Inc.). The yeasts were grown on SD-Leu-Trp plates at 30°C ...
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid assays were based on the Matchmaker LexA two-hybrid system (Clontech). BPM genes were cloned into the pGlida vector ...
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bioRxiv - Biochemistry 2024Quote: ... Screening was performed by stepwise transformation of each vector into Y2H Gold yeast (Takara). Co-transfected yeasts were grown in liquid culture starting from glycerol stocks ...
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bioRxiv - Molecular Biology 2021Quote: ... The identified interactions were further confirmed using the Matchmaker Gold yeast two-hybrid system (Clontech) or the DUALhybrid system (Dualsystems Biotech) ...
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bioRxiv - Plant Biology 2020Quote: Y1H assay was performed using Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, USA). The nucleotide sequences of promoter region of selected key defense genes having potential RAV1 binding motifs were retrieved by using online tool (https://bioinformatics.psb.ugent.be/plaza/) ...
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bioRxiv - Plant Biology 2020Quote: ... Combinations of LexA BD- and AD- fusion plasmids were cotransformed into yeast strain EGY48 (Clontech) containing the reporter plasmid p8op-LacZ ...
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bioRxiv - Plant Biology 2022Quote: ... Growth was determined as described in the Yeast Two-Hybrid System User Manual (Clontech, Japan).
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bioRxiv - Cell Biology 2022Quote: Yeast-two-hybrid analysis was performed with the MATCHMAKER GAL4 Two-Hybrid System 3 (Clontech) essentially as described (39) ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybrid assays were carried out using the Matchmaker Two-Hybrid System Kit (Clontech). The AtCGL160 CDS without the signal peptide (see Supplemental Table S2 for primer information ...
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bioRxiv - Microbiology 2020Quote: The yeast two-hybrid assays were performed in strain Saccharomyces cerevisiae Y2HGold (Takara Bio USA). Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research) ...
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bioRxiv - Cancer Biology 2021Quote: ... Bait and prey vectors were co-transformed into the yeast strain AH109 (Clontech/Takara, USA), using a standard high-efficiency transformation protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Bait and prey vectors were co-transformed into the yeast strain AH109 (Clontech/Takara, USA), using a standard high-efficiency transformation protocol ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid assays were performed by the Matchmarker GAL4-based two-hybrid System (Clontech). Constructs were co-transformed into the yeast strain AH109 by the lithium acetate method (Gietz & Woods ...
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bioRxiv - Plant Biology 2020Quote: ... Yeast two-hybrid assay and one-hybrid assay were performed following the manufacturer’s instructions (Clontech).
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bioRxiv - Cell Biology 2020Quote: Yeast two hybrid analysis was performed according to the manufacturer guidelines (Matchmaker; Clontech Laboratories, Inc.). Genes of interest were cloned from wildtype (N2 ...
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bioRxiv - Genetics 2022Quote: Y2H were performed using the Gold Yeast Two-Hybrid System according to manufacturer’s manual (Clontech). CDSs from different genes with combination of pGADT7 and pGBKT7 were co-transformed into the yeast strain AH109 ...
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bioRxiv - Plant Biology 2022Quote: ... All matings were performed as detailed in the Yeast Protocols Handbook (Clontech, Mountain View, California) using the 96-well plate format ...
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bioRxiv - Plant Biology 2022Quote: We used the GAL4-based Matchmaker Gold Yeast 2-Hybrid System (Clontech, Mountain View, California) for all Y2H and Y3H assays ...
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bioRxiv - Plant Biology 2022Quote: ... All transformations were performed as detailed in the Yeast Protocols Handbook (Clontech, Mountain View, California). For Y2H ...
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bioRxiv - Plant Biology 2022Quote: ... The Y2H assays were carried out in the Matchmaker Gold Yeast Two-Hybrid System (Clontech). The pairs of corresponding recombinant constructs fused with pGBKT7 BD and pGADT7 AD were co-transformed into the Y2H Gold yeast competent cells ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast one-hybrid (Y1H) assays were performed according to the manufacturer’s instruction (Clontech, CA, USA). The coding region of PIF4 was amplified and cloned into the yeast GAL4 activation domain (GAL4 AD ...
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bioRxiv - Plant Biology 2023Quote: ... The yeast transformants were grown on nutrient-restricted (without Trp, Leu, His, Ade) mediums (Clontech) 3-5 days to assess interactions between various protein combinations.
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bioRxiv - Plant Biology 2023Quote: ... and Y1H analysis was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The transformed yeast strains were grown on SD/-Leu and SD/-Leu/AureobasidinA media (Takara) and Y1H analysis was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara ...