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Citations for Takara Bio :
251 - 300 of 2356 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The transformed yeast strains were grown on SD/-Leu and SD/-Leu/AureobasidinA media (Takara) and Y1H analysis was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... Positive yeast colonies were selected and confirmed by using different synthetic drop-out (SD) (Takara) media prepared according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The detailed protocol was described in the manufacturer’s handbook (Yeast protocols handbook; PT3024-1; Clontech).
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bioRxiv - Molecular Biology 2024Quote: ... yeast two-hybrid (Y2H) assays were performed using the Matchmaker Gold Y2H System (Clontech, USA). The full-length coding sequences of BnC07MYB3a ...
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bioRxiv - Plant Biology 2021Quote: ... We use the yeast (Saccharomyces cerevisiae) strain AH109 in the MATCHMAKER GAL4 Two-Hybrid System (Clontech), in which HIS3 ...
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bioRxiv - Cancer Biology 2021Quote: Yeast two-hybrid assay was conducted using Matchmaker GAL4 two-hybrid system 3 (Clontech/Takara, USA). Bait and prey vectors were co-transformed into the yeast strain AH109 (Clontech/Takara ...
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bioRxiv - Cancer Biology 2021Quote: Yeast two-hybrid assay was conducted using Matchmaker GAL4 two-hybrid system 3 (Clontech/Takara, USA). Bait and prey vectors were co-transformed into the yeast strain AH109 (Clontech/Takara ...
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bioRxiv - Neuroscience 2020Quote: ... a Y2H screening was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... a Y2H screening was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Y1H assays were performed using the Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, USA). The sequence of LhWRKY44 was inserted into the vector pGADT7 as prey ...
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bioRxiv - Molecular Biology 2023Quote: Protein interactions in vivo in yeast were assayed using the ‘Matchmaker Gold Two-hybrid System’ (Clontech) using the protocols provided by the supplier ...
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bioRxiv - Plant Biology 2023Quote: ... cDNAs were cloned into Gateway-compatible yeast two-hybrid vectors based on pGADT7 and pGBKT7 (Clontech) or into the pAG416GPD-ccdB-HA vector (Alberti et al. ...
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bioRxiv - Plant Biology 2023Quote: Transformation of Saccharomyces cerevisiae strain HF7c was performed as described in the Yeast Protocols Handbook (Clontech), with transformants selected on synthetic dropout (SD ...
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid analysis was performed using the MatchMaker GAL4 Two-Hybrid System 3 (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... yeast two-hybrid analyses were performed using the Matchmaker GAL4 Two-Hybrid System 3 (Clontech Laboratories). S ...
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bioRxiv - Plant Biology 2022Quote: ... bHLH39-1 and bHLH39-2 fused to the BD were co-transformed into yeast Y2HGold (Clontech). Transformants were grown on SD-Leu-Trp plates ...
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bioRxiv - Microbiology 2022Quote: Protein-protein interactions were assayed with the Matchmaker yeast two-hybrid system (Clontech, Mountain View, CA). ORFs of AoHse was amplified from first-strand cDNA of A ...
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bioRxiv - Neuroscience 2024Quote: ... Series of combinations of bait and prey constructs were cotransformed into the yeast strain NMY51 (Clontech), and different concentrations of bacterial liquid were growing onto SD/-Trp/-Leu plates for 3 d at 30°C ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μM ROCK inhibitor (Y-27632; Selleckchem, Cat. No. S1049) and 2 μg/mL doxycycline (Clontech, Cat. No. 631311). Media was changed daily during this stage.
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bioRxiv - Biochemistry 2020Quote: ... pGAD plasmids were transformed into PJ49-4a yeast and were selected on leucine-dropout medium (Clontech, #630414). pTEF or pTEFdual plasmids were included to express proteins in trans and were transformed with pOBD plasmids and selected on media lacking tryptophan and uracil (Sunrise Science Products ...
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bioRxiv - Developmental Biology 2020Quote: Yeast two-hybrid experiments were carried out using the Matchmaker GAL4 Two-Hybrid System 3 (Takara Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Yeast two-hybrid experiments were carried out using the Matchmaker GAL4 Two-Hybrid System 3 (Takara Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cerevisae: All Yeast-two hybrid plasmids were generated on the basis of the Matchmaker III System (Clontech Laboratories Inc. ...
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bioRxiv - Molecular Biology 2022Quote: ... we used the Matchmaker® Gold Yeast One-Hybrid Library Screening System (Clontech, Mountain View, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The screen was performed followed by the Matchmaker Gold Yeast Two-Hybrid System User Manual (Takara, 071519). One large Y2HGold[pGBKT7-Dunk] yeast colony was picked from a fresh SD/-Trp plate and inoculated into 50 ml of SD/-Trp liquid media ...
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bioRxiv - Microbiology 2020Quote: ... media (0.67% yeast nitrogen base, 0.5% glucose, 0.01% adenine hemisulfate salt) supplemented with -Leu DO supplement (Clontech) (SD-Leu ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid analysis was employed using the MatchMaker GAL4 Two-Hybrid System 3 (Takara Bio, Japan) as previously described (Umezawa et al. ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast transformants were selected by growth on synthetic dropout nutrient medium SD/-Leu/-His/-Ade/-Trp (Clontech) supplemented with 40mg/LX-α-gal (5-bromo-4-chloro-3-indolyl-a-D-galactopyranoside ...
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bioRxiv - Plant Biology 2023Quote: ... pDESTTM32RAPTOR1BcDNAwas co-transformed either with pDESTTM22FLZ14cDNA or pDESTTM22S6K1cDNA plasmids into the yeast strain Y2HGold (Takara Bio, USA) as previously described (Paiano et al. ...
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bioRxiv - Molecular Biology 2022Quote: The yeast two hybrid assay was performed as indicated in MATCHMAKER GAL4 two-hybrid system 3 (Clontech). The protein coding regions of genes used in this study were amplified from Guy11 cDNA with primer pairs listed in Table 1.1 ...
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bioRxiv - Cell Biology 2022Quote: ... cereviseae strains Y2HGold and Y187 were used for Yeast Two Hybrid assay (Takara Bio, Mountain View, USA).
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid assays were carried out using the Matchmaker Gold system (Takara™ Bio USA, Inc) with yeast strain AH109 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The selected yeast strains were seeded in SD medium with Dropout (DO) supplement-His (Clontech, CA, US) on a sterile black clear-bottom 384-well plate (Corning ...
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bioRxiv - Molecular Biology 2024Quote: The Matchmaker Gold Y1H system was used for yeast (S. cerevisiae) one-hybrid assays (Takara, https://www.takarabiomed.com.cn). The reporter strains were created by putting the MdKASI ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were diluted 30 or 60 times in 100 µl mTeSR with 1:1000 Y-27632 (STEMCELL, 72302) and 2µg/ml doxycycline (Clontech, 631311) and the cell suspension was added to a well containing the transfection complexes ...
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bioRxiv - Biophysics 2021Quote: ... AY457063.1) and PIKfyve-KYA hyperactive mutant (E1620>K, N1630>Y, S2068>A) were cloned into PCMV-HA-N vector (Clontech) and gifted by Lois Weisman lab ...
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bioRxiv - Plant Biology 2020Quote: ... 10 AtCBLs prey vectors were co-transformed into the yeast strain AH109 following manufacturer’s instructions (Clontech, CA, USA). The empty vectors pTOOL27 and pTOOL28 were also co-transformed into yeast with each prey or bait plasmid respectively to confirm that bait does not autonomously activate the reporter genes in the absence of the prey protein.
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bioRxiv - Plant Biology 2022Quote: The Y2H assays were performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech, Mountain View, CA, USA). The full-length and truncated coding regions of WL1 were amplified and cloned into the yeast expression vector pGADT7 (Clontech ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant yeast strain was confirmed by PCR conducted with Matchmaker™ Insert Check PCR Mix (TAKARA, Japan). The full length of SlBES1.8 coding sequence was cloned into pGADT7 plasmid and subsequently transformed into the recombinant yeast strain ...
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bioRxiv - Biochemistry 2021Quote: ... All yeast two-hybrid screens and assays were performed using the BD Matchmaker system (BD Biosciences, Clontech Laboratories) utilizing the Saccharomyces cerevisiae strain AH109 essentially as described earlier (Stephan et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Bait and prey plasmids were co-transformed into the yeast strain AH109 according to the manufacture’s introduction (Clontech). Transformants were selected on SD (-Leu/-Trp ...
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bioRxiv - Plant Biology 2021Quote: Protein-protein interactions via yeast-two-hybrid (Y2H) assays were performed as described in the Matchmaker protocol (Clontech). HvRACB (amino acids 1-193 ...
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bioRxiv - Biochemistry 2020Quote: One colony was used to inoculate 10mL of selection media (6.9 g/L Yeast Nitrogen Base without amino acids, 0.62g/L Clontech -Leu/-Trp/-Ura dropout supplement ...
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bioRxiv - Biochemistry 2020Quote: ... Transformed cells were plated on a selection agar (6.9g/L yeast nitrogen base without amino acids, 0.62g/L Clontech -Leu/-Trp/-Ura dropout supplement ...
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bioRxiv - Plant Biology 2021Quote: ... URA3: GAL1UAS–Gal1TATA–LacZ MEL1) via the Yeastmaker™ Yeast Transformation System 2 kit (Clontech, Mountain View, USA). An agarose gel image of the cDNA library is shown in Fig ...
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bioRxiv - Plant Biology 2023Quote: The interaction between PIF4 and MPK4 was examined using the Matchmaker gold yeast two-hybrid system (Takara Bio). PIF4 and MPK4 cDNA were cloned in the pGADT7 and pGBKT7 vectors ...
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bioRxiv - Plant Biology 2020Quote: Y2H screening was performed using Matchmaker Gold Yeast Two-Hybrid System (Takara Bio USA, Inc., Mountain View, US-CA). For bait AP_SAP11-like_PM19 or AP_SAP11-like_PM19Δ40-56 was cloned into pGBKT7 (TRP1 nutritional marker ...
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A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transportersbioRxiv - Cell Biology 2021Quote: ... pGADCg- and pGBKCg-based plasmids containing the indicated protein fusions were transformed into the yeast strain Y2HGOLD (Takara Bio), and double transformants were selected by growth on SD media lacking leucine and tryptophan ...
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bioRxiv - Cell Biology 2022Quote: ... the relevant AD and BD constructs were co-transformed into the auxotrophic yeast strain AH109 using the polyethylene glycol/lithium acetate method according to the manufacture’s manual (Clontech). Yeast cells haboring the relevant combinations of constructs were dotted on plates with double (-Leu-Trp) ...
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bioRxiv - Plant Biology 2021Quote: ... Bait (pGBKT7) and prey (pGADT7) constructs were co-transformed into the yeast strain AH109 according to manufactural instruction (Clontech). The resulting transformants were spotted on SD (−Leu ...