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Citations for Takara Bio :
351 - 400 of 2356 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... the pSTT91 and pGAD424 plasmids cloned with potential interactors were introduced into the Saccharomyces cerevisiae strain L40 and grown for 2 days at 30°C on a leucine- and tryptophan- deficient synthetic defined premixed yeast growth medium (SD-Leu-Trp, TaKaRa Clontech)(Hollenberg et al. ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant plasmid was used as bait to screen the cDNA library using the Matchmaker™ Gold Yeast Two-Hybrid Library Screening System kit (Cat. no. 630489, Clontech).
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bioRxiv - Neuroscience 2020Quote: ... Mated yeast clones were selected using minimal synthetic defined (SD) medium with double dropout (Leu− and Trp−) supplement (Clontech Takara Bio) containing Aureobasidin A and X-α-Gal as the blue-colored colonies ...
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bioRxiv - Neuroscience 2020Quote: ... Mated yeast clones were selected using minimal synthetic defined (SD) medium with double dropout (Leu− and Trp−) supplement (Clontech Takara Bio) containing Aureobasidin A and X-α-Gal as the blue-colored colonies ...
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bioRxiv - Plant Biology 2021Quote: ... The Y2H assay was performed following the manual provided in the kit “Matchmaker Gold Yeast Two-Hybrid” (Clontech, Mountain View, USA). The list of VirE2 interacting clones has been given in Fig.S1.
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bioRxiv - Microbiology 2021Quote: ... The amplified PCR product was verified by sequencing and then cloned into modified XW55 yeast dual expression vector65 in the highlighted SpeI and PmlI sites downstream of the ADH2 promoter by yeast recombinase technology [protocolYeastmaker™ YeastTransformation System 2 (Clontech)] and according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant constructs were introduced into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: The recombinant pGBKT7-PGRP3 bait construct was transformed into yeast strain Y2HGold via the lithium acetate method per manufacturer protocol (Takara Bio). Transformants were cultured on SD/-Trp (SDO ...
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bioRxiv - Plant Biology 2022Quote: ... in pGADT7 were combined with each other and with empty control plasmids using the Matchmaker™ Gold Yeast Two-Hybrid System (Clontech). The vectors were co-transformed into the Y2Hgold MATa Yeast strain ...
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bioRxiv - Plant Biology 2022Quote: ... The yeast strain Y2H AH109 was cotransformed with the prey and corresponding bait vectors following the protocol provided by the Yeastmaker™ yeast transformation system (Clontech). The yeast transformants were grown on SD/-Trp/-Leu medium ...
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bioRxiv - Plant Biology 2023Quote: ... were used to co-transform yeast strain AH109 and colonies carrying both vectors were selected on SD medium without tryptophan (-W) and leucine (-L) (TaKaRa 630317) at 28°C ...
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bioRxiv - Plant Biology 2022Quote: ... StNPR1 or StNPR3/4 cds) construct combinations were transformed into the Y2H Gold strain using the Matchmaker Gold Yeast Two-Hybrid System (Clontech, USA) and the transformants selected on control SD media without Leu and Trp (-L-W) ...
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bioRxiv - Microbiology 2024Quote: ... Yeast cells containing pGBKT7PA28γ were grown in yeast extract-peptone-dextrose medium and transfected with a human foetal brain plasmid library based on pACT2 (Clontech Takara). Clones (22.4 × 106 ...
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bioRxiv - Cell Biology 2020Quote: ... Verification of positive colonies was achieved by co-transforming wild-type or YR-mutant TMEM39A loop domain (in pGBKT7 Vector) with genes of interest (in pGADT7 Vector) following the instruction of YeastMaker™ Yeast Transformation System 2 (Clontech, 630439) as well as plasmids from re-cloned cDNA.
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bioRxiv - Cell Biology 2020Quote: The yeast strain AH109 was co-transfected with bait vector full-length human SNX4 or Lamin cloned into pGBKT7 (Clontech, Oxford, UK) against a pray library of FL SNX-BARs ...
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... NRC3 and NRC4 CC and NB-ARC domain truncations and ran a yeast two-hybrid experiment using the Matchmaker Gold system (Takara Bio USA). Plasmid DNA encoding AVRcap1b (negative control ...
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bioRxiv - Plant Biology 2021Quote: ... For expressing the GAL4BD-SMXL5 fusion protein in yeast the open reading frame of the SMXL5 gene was cloned into XmaI/BamHI sites of the pGBKT7 plasmid (Clontech, Palo Alto) resulting in pEW6 ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybrid techniques were performed according to the yeast protocols handbook and the Matchmaker GAL4 Two-hybrid System 3 manual (both Clontech, Heidelberg, Germany) using the yeast reporter strains AH109 and Y187 ...
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bioRxiv - Plant Biology 2021Quote: ... Y2H screen was performed to screen the apple library according to the user manual of Matchmaker™ Gold Yeast Two Hybrid System (Clontech, Japan) by using Saccharomyces cerevisiae strain Y2H Gold.
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bioRxiv - Genetics 2020Quote: ... Verification of positive colonies was achieved by co-transformation of extracted bait and prey plasmids following the instruction of YeastMaker™ Yeast Transformation System 2 (Clontech, 630439) and by bait/prey plasmids with re-cloned cDNA.
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bioRxiv - Plant Biology 2020Quote: ... and LacZ reporter genes was examined with yeast AH109 transformants on SD/−Trp/−His and SD/−Trp/−Ade media (Clontech Inc., USA). The fix composition of SD plates contained 0.17 g yeast nitrogen base (YNB) ...
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid techniques were performed according to the yeast protocols handbook and the Matchmaker GAL4 Two-hybrid System 3 manual (both Clontech, Heidelberg, Germany) using the yeast reporter strains AH109 and Y187 ...
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bioRxiv - Cell Biology 2022Quote: ... Matings between the Y2HGold and Y187 strains carrying the appropriate constructs were performed in 0.5 mL of 2X YPDA in the presence of the corresponding antibiotics following manufacturer’s instructions (Clontech’s Matchmaker Gold Yeast Two-Hybrid System, Clontech by Takara Bio, Mountain View, USA). Yeast cells were cultured at 30 ºC for 24 h and plated in SD-Trp/-Leu/X-alpha-Gal agar plates ...
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bioRxiv - Cell Biology 2022Quote: ... positive control (pGBKT7-53) and negative control bait plasmids (pGBKT7-Lam) were transformed into yeast strain Y2HGold (Takara Bio, Mountain View, USA) while pGADT7-CmVPS41s and pGADT7-T control prey plasmids were transformed into yeast strain Y187 following the Yeastmaker Yeast Transformation System 2 instructions (Clontech by Takara Bio ...
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bioRxiv - Plant Biology 2022Quote: Direct protein-protein interaction was tested by Y2H technique according to the yeast protocols handbook and the Matchmaker GAL4 Two-hybrid System 3 manual (both Clontech, Heidelberg, Germany). Yeast strain Y190 was co-transformed with respective plasmids ...
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bioRxiv - Plant Biology 2023Quote: ... The coding region of PIF4 was amplified and cloned into the yeast GAL4 activation domain (GAL4 AD) of pGADT7-Rec2 (Clontech, CA, USA) prey vector ...
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bioRxiv - Plant Biology 2023Quote: A Y2H library screen was performed as outlined in the Matchmaker Gold “Mate and Plate” Yeast Two-Hybrid System (Takara Bio Inc.) using pGBKT7-CML13 as the bait in yeast strain AH109 ...
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bioRxiv - Plant Biology 2023Quote: For Yeast-two-Hybrid (Y2H) experiments coding DNA sequences were cloned into pDONR207 and recombined into pGDAT7® or pGBKT7® vectors (Clontech). Plasmids were transformed into the AH109 yeast strain by the lithium acetate (LiAc ...
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bioRxiv - Molecular Biology 2024Quote: ... sites flanking gene-specific nucleotides and cloned first into an entry vector pDONR207 and then into destination yeast two-hybrid vectors pGADT7 or pGBKT7 (Clontech, Takara Bio USA) using Gateway cloning kit (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... The relevant vectors were co-expressed in Y1H yeast cells and screened with varying concentrations of Aureobasidin A (AbA, TaKaRa, Beijing, China).
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bioRxiv - Cell Biology 2022Quote: ... Transformants were selected on synthetic media containing yeast nitrogen base (YNB, Difco) supplemented with 2 % glucose and DropOut supplements lacking uracil (Clontech, Mountain View, CA). Single colonies were used to inoculate 5 mL liquid YNB media supplemented with 2 % glucose and DropOut supplements lacking uracil ...
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bioRxiv - Synthetic Biology 2020Quote: Yeast with the uracil auxotrophy were cultivated in complete synthetic media without uracil prepared with -His-Ura dropout supplement (Clontech, Mountain View, CA) according to manufacturer’s instructions and supplemented with 20 g/mL histidine and 80mg/L adenine hemisulfate (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... These plasmids were transformed into the yeast strain AH109 for testing protein-protein interaction using the Matchmaker Gold system (Takara Bio, Kusatsu, Japan). Yeast transformation and growth were performed as described in [Pecher et al. ...
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bioRxiv - Plant Biology 2023Quote: ... selections and LacZ reporter assays were made according to the Matchmaker Gold Yeast Two-Hybrid System user manual (Clontech; Mountain View, Ca, USA).
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bioRxiv - Plant Biology 2023Quote: ... The yeast two-hybrid and one-to-one confirmation experiments were performed according to Matchmaker™ Gold Yeast Two-Hybrid System User Manual (Clontech, https://www.clontech.com/). Primers for vector construction were listed in Table S8.
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bioRxiv - Plant Biology 2024Quote: ... The dilutions were plated on selective -LT medium as a growth control and on -LTH medium (7.6 g/L Yeast Nitrogen Base [YNB], 20 g/L glucose, 0.62 g/L DO supplement -leu -trp -his [Clontech], 15 g/L agar) for the protein interaction assay ...
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bioRxiv - Molecular Biology 2024Quote: ... sites flanking gene-specific nucleotides and cloned first into an entry vector pDONR207 and then into destination yeast two-hybrid vectors pGADT7 or pGBKT7 (Clontech, Takara Bio USA) using Gateway cloning kit (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Matings between the Y2HGold and Y187 strains carrying the appropriate constructs were performed in 0.5 mL of 2X YPDA in the presence of the corresponding antibiotics following manufacturer’s instructions (Clontech’s Matchmaker Gold Yeast Two-Hybrid System, Clontech by Takara Bio, Mountain View, USA). Yeast cells were cultured at 30 ºC for 24 h and plated in SD-Trp/-Leu/X-alpha-Gal agar plates ...
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bioRxiv - Microbiology 2024Quote: ... The resulting vectors were transformed into yeast strain AH109 according to Matchmaker™ GAL4 Two-Hybrid System 3 protocol (Clontech, Mountain View, CA, USA).
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bioRxiv - Cell Biology 2021Quote: ... An adult mouse retina cDNA library was cloned in pGADT7 (carrying a Trp1 selection gene) and transformed in AH109 yeast strain (containing His3, Ade2, lacZ and Mel1 selections; Clontech, BD Biosciences, Pao Alto, CA). Cpne4 vWA domain was cloned into pGBKT7 (carrying Leu2 selection gene ...
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bioRxiv - Plant Biology 2022Quote: Y2H protein interaction assays were performed according to the manufacturer’s instructions as described in the Yeast Protocols Handbook (Clontech; TaKaRa Bio USA, Inc., Forster, CA, USA). Constructs that contained NZR3.1 ...
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bioRxiv - Cell Biology 2023Quote: Gene encoding ARF-1 were amplified from cDNA (cDNA synthesized from RNA isolated from Hela cells) using high fidelity DNA polymerase enzyme from TaKaRa and cloned with N-terminal flag and HA tag in mammalian expression vector pcDNA3 flag HA Akt1 plasmid obtained from addgene(1477) ...
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bioRxiv - Molecular Biology 2021Quote: GroEL from Escherichia coli was purchased from TaKaRa Bio Inc ...
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bioRxiv - Cell Biology 2020Quote: ... cDNAs from primary hepatocytes were purchased from TAKARA Shuzo ...
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bioRxiv - Microbiology 2021Quote: ... from TaKaRa Bio ...
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bioRxiv - Cancer Biology 2020Quote: ... Human total RNA from different normal tissues from Clontech (Human Total RNA Master Panel II,Cat# ...
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bioRxiv - Cancer Biology 2023Quote: ... TRE3G promoter from pLVX-TRE3G plasmid purchased from Clontech was PCR amplified with Kpn1 in forward primer and Aar1 in reverse primer ...
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bioRxiv - Microbiology 2022Quote: ... Nup98 was amplified from a HeLa cDNA library from Clontech/Takara ...
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bioRxiv - Microbiology 2021Quote: ... EGFP (from Clontech) or Fusion Red ((43 ...
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bioRxiv - Biochemistry 2022Quote: ... Xfect from Takara Bio is characterized as a biodegradable transfection polymer suitable for both CHO and HEK ...