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Citations for Takara Bio :
301 - 350 of 2356 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybridization (Y2H) assays were performed according to the manufacture’s procedure (Clontech Laboratories Inc., Palo Alto, CA, USA). For each isozyme ...
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bioRxiv - Genetics 2022Quote: ... color change was measure at OD578 and β-galactosidase units were calculated as described (Yeast Protocol Handbook, Takara Bio).
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bioRxiv - Plant Biology 2022Quote: ... plasmids were transformed into yeast strain AH109 by the lithium chloride–polyethylene glycol method according to the manufacturer’s manual (Clontech). The transformants were selected on SD-Leu-Trp plates ...
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bioRxiv - Plant Biology 2022Quote: Y2H protein interaction assays were performed according to the manufacturer’s instructions as described in the Yeast Protocols Handbook (Clontech; TaKaRa Bio USA ...
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bioRxiv - Plant Biology 2024Quote: ... the relevant combinations of constructs were co-transformed into the auxotrophic yeast strain AH109 using the polyethylene glycol/lithium acetate method according to the manufacturer’s manual (Clontech). Yeast cells were dotted on the plates of double (-Leu-Trp ...
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bioRxiv - Molecular Biology 2024Quote: ... The ORF of HvHsfA6a was amplified and fused to GAL4 BD in the yeast vector pGBKT7 (Clontech, CA, USA), and the resulting constructs was designated as pGBKT7:HvHsfA6a ...
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bioRxiv - Bioengineering 2023Quote: ... was cloned into the prey vector pPR3-N and co-transformed into yeast strain NMY51 cells together with pDHBⅠ-GhERF105a plasmid according to the Matchmaker user’s manual protocol (Clontech). Yeast transformants were selected by growth on synthetic dropout nutrient medium SD/-Leu/-His/-Ade/-Trp (Clontech ...
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bioRxiv - Genetics 2023Quote: ... The transformation process followed the protocol described in the Yeastmaker™ Yeast Transformation System 2 User Manual (Takara Bio), yielding approximately 2 × 106 and 7 × 106 transformants for LE9 and BLX strains ...
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae was carried out by using the lithium acetate method of Yeastmaker™ Yeast Tansformation System (CLONTECH Laboratories, Inc.). The plasmid of pICASA was cleaved by XbaI and integrated into the chromosomal DNA of S ...
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bioRxiv - Plant Biology 2024Quote: ... Different combinations of bait and prey plasmids were transformed into yeast strain AH109 using PEG/LiCl method (Clontech, USA), and the transformants were selected on synthetic dropout (SD ...
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bioRxiv - Microbiology 2024Quote: ... yeast (OD 0.8) were serial diluted at 10-fold on SC minimal dropout (-Trp, -Ura, -Leu) agar plates (Clontech) at 30 LJ.
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bioRxiv - Plant Biology 2024Quote: ... The respective combinations of GAD-fusion and GBD-fusion plasmids were co-transformed into yeast strain Y2H-Gold (Clontech) and colonies grown on synthetic defined (SD ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were cloned in plasmids derived from the 2 hybrid vectors pGADT7 (GAL4-activating domain) and pGBKT7 (GAL4-binding domain) creating N terminal fusions and transformed in yeast haploid strains Y187 and AH109 (Clontech), respectively ...
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bioRxiv - Plant Biology 2022Quote: ... these plasmids were co-transformed into the Y2H GOLD strain in an activation domain (AD)-BD coupled manner (Yeast Protocols Handbook, PT3024-1; Clontech). The primers used are listed in Supplemental Table S1.
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bioRxiv - Cell Biology 2021Quote: Interactions between CDK-2 and COSA-1 were assayed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech PT4084-1). CDK-2 and COSA-1 cDNAs were amplified from a C ...
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bioRxiv - Plant Biology 2021Quote: ... containing a pBridge bait vector or diploid yeast strains generated by mating a Y2HGold strain containing a bait vector with a Y187 strain (Clontech) containing the pGADT7 vector ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybrid assays were performed as described (Cesari et al., 2013) using the Matchmaker Gold Yeast Two-hybrid system (Clontech).
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bioRxiv - Neuroscience 2020Quote: ... was verified by co-transformation of the bait (pAS2-1-APP or pAS2-1-AICD) and the prey plasmid (HB-EGF-pACT2) in the yeast strain AH109 (Clontech). First ...
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bioRxiv - Physiology 2021Quote: ... the pSTT91 and pGAD424 plasmids cloned with potential interactors were introduced into the Saccharomyces cerevisiae strain L40 and grown for 2 days at 30°C on a leucine- and tryptophan- deficient synthetic defined premixed yeast growth medium (SD-Leu-Trp, TaKaRa Clontech)(Hollenberg et al. ...
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bioRxiv - Plant Biology 2022Quote: ... The full-length and truncated coding regions of WL1 were amplified and cloned into the yeast expression vector pGADT7 (Clontech) to produce pGADT7-WL1 ...
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bioRxiv - Plant Biology 2022Quote: ... PtoATPE and PtoPSBB) were co-transformed into the Y1HGold yeast strain using the Matchmaker One-Hybrid Library Construction and Screening Kit (Clontech), respectively ...
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bioRxiv - Plant Biology 2021Quote: ... All combinations were transferred to the SD/-Leu-Trp medium by the yeast strain Y2H (Clontech, Mountain View, CA, USA), and then transferred to the SD/-Leu-Trp medium ...
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bioRxiv - Molecular Biology 2020Quote: ... Y1H reporter plasmids pHISi-1-Sph2 were linearized by XhoI and stably integrated into the non-functional HIS3 locus of Yeast YM4271 by homologous recombination following the manufacturer’s instructions (Clontech, CA). To control basal expression of HIS3 reporter gene ...
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bioRxiv - Plant Biology 2020Quote: The yeast two hybrid (YTH) experiments were performed in three replicates using the Matchmaker Gal4-based two-hybrid system (Clontech) and the Saccharomyces cerevisiae strain Y2HGold as described in (11) ...
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bioRxiv - Molecular Biology 2020Quote: PJ69-4a strain of Saccharomyces cerevisiae was used for the expression of proteins of interest using the Matchmaker Gal4-based yeast two hybrid system (Clontech). Tested bait/prey combinations were co-transformed into PJ69-4a and cultivated at 28°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified ds CDNA was finally co-transformed with pGAD-T7 Rec into competent Y187 using Yeastmaker yeast transformation system (Takara, Clontech) and plated on SD-Leucine agar media ...
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bioRxiv - Microbiology 2022Quote: ... A tomato Mate & Plate library was constructed and used for screening with the Matchmaker Gold Yeast Two-Hybrid System (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 10 ng of linearized pDEST32 empty vector was co-transformed with 3 μl of PCR product to achieve recombinational cloning by gap-repair in Y2H Gold yeast strain (Clontech) expressing AD-fused CP204L (pPC86 vector) ...
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bioRxiv - Cell Biology 2022Quote: ... The yeast strain AH109 was transformed with the constructs in destination vectors using lithium acetate transformation (Yeast Protocols Handbook PT3024-1, Clontech). The transformants were grown on synthetic defined medium (0.67% yeast nitrogen base ...
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bioRxiv - Microbiology 2022Quote: ... Transformed yeast cells were cultured in a synthetic-defined (SD) medium without tryptophan (SD/-Trp broth, Takara Bio USA Inc.) and induced in SD/-Trp broth with 2% raffinose as an alternative carbon source by the addition of 1 to 3% galactose ...
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bioRxiv - Molecular Biology 2022Quote: Y1H library screening was performed by using the Matchmaker Gold Yeast One-Hybrid Library and Screening kit (Clontech,CA, USA). The promoter fragments of CaPR1 were inserted in the pAbAi vectors ...
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bioRxiv - Plant Biology 2022Quote: ... β-galactosidase activity in liquid cultures was assayed using CPRG as substrate as described in the Yeast Protocols Handbook (Takara).
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bioRxiv - Plant Biology 2022Quote: ... The SlBZR1 CDS was introduced into the pGBKT7 vector and then used as bait to screen the cDNA library based on the Matchmaker Gold Yeast Two-Hybrid System (Clontech). Yeast transformants were exhaustively selected on SD/-Ade/-His/-Leu/-Trp/X-α-Gal medium ...
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bioRxiv - Plant Biology 2024Quote: The HIS3 and ADE2 reporter genes were employed to assess the protein interactions in the yeast strain Y2HGold (Takara Bio). Yeast transformation was carried out using the lithium acetate (LiAc)-mediated method ...
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bioRxiv - Plant Biology 2024Quote: ... two-hybrid assay was conducted according to the manufacturer’s instructions for the Match-maker Gold Yeast Two-Hybrid System (Clontech, USA). To generate the bait construct BD-GhCPK28 ...
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bioRxiv - Plant Biology 2023Quote: ... Direct protein-protein interaction was confirmed by co-transformation of the respective plasmids into the yeast strain AH109 using the Matchmaker GAL4 Two-hybrid System (Clontech), followed by a selection of transformants on 2% SD (-Leu/-Trp ...
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bioRxiv - Cell Biology 2024Quote: ... Pairs of pGBKT7 and pGADT7 plasmid were co-transformed into the yeast strain AH109 following the MatchmakerTM GAL4 Two-Hybrid System instructions (Clontech). Primary transformants were selected on synthetic drop-out (SD ...
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bioRxiv - Bioengineering 2023Quote: ... The recombinant plasmid of pGBKT7-GhERF105a/d and plasmid with empty vector were transformed respectively into strain AH109 yeast cells according to the manufacturer’s instructions (Clontech, USA). The transactivation activity was determined by the growth on SD/-Trp and SD/-Trp/-X-a-gal (5-bromo-4-chloro-3-indolyl-α-D-galactopyranoside ...
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bioRxiv - Plant Biology 2023Quote: ... pYES2-SiHDA9 and empty pYES2 vector (EV) were transformed individually into Saccharomyces cerevisiae strain W303 using Yeastmaker Yeast Transformation System (Clontech). The transformed cells were screened on SD/-ura medium with 2% (w/v ...
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bioRxiv - Plant Biology 2023Quote: ... The LexA fusion constructs (bait) and the activation domain fusion constructs (prey) were co-transformed into yeast strain EGY48[p8op-lacZ] (CLONTECH). Clones containing both constructs were selected on medium lacking His ...
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bioRxiv - Microbiology 2024Quote: ... Yeast cells containing pGBKT7PA28γ were grown in yeast extract-peptone-dextrose medium and transfected with a human foetal brain plasmid library based on pACT2 (Clontech Takara). Clones (22.4 × 106 ...
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bioRxiv - Biochemistry 2024Quote: ... Plant targets identified in the yeast-two hybrid screen were cloned as translational fusions with the activation domain in pB42AD (Clontech) for verification ...
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bioRxiv - Genomics 2024Quote: ... The pGADT7 derivatives that express GAL4-AD fused to either Pmc4 or Cnd2 (GAL4-AD-Pmc4 or GAL4-AD-Cnd2) in addition to the LEU2 marker gene were independently transformed into the Mat a Y2HGold budding yeast strain (Clontech). After crossing Mat α strain with Mat a strain on YEA plates ...
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bioRxiv - Developmental Biology 2024Quote: ... MS2-orf147 hybrid RNA expressing in pPRP1 vector and identified PPRs expressing in prey vector (pGADT7) All three components were then co-transformed into yeast host (Saccharomyces cerevisiae strain Y187 (Takara) at equimolar concentrations in DDO/X/A [SD/–Leu/–Trp/X-a-Gal/AbA] media (containing kanamycin to prevent bacterial contamination ...
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bioRxiv - Plant Biology 2024Quote: The full-length FLP1 cDNA in pENTR/D-TOPO was cloned into pDEST_GBKT7 vector85 by Gateway reaction and transformed into Y2HGold yeast strain (Takara Bio). The resultant yeast was cultured in synthetic defined medium (–W) ...
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bioRxiv - Microbiology 2024Quote: ... Dropout medium and plates were prepared with DifcoTM Yeast Nitrogen Base without Amino Acid (Becton Dickinson) and DO supplements (Clontech) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... The yeast one-hybrid (Y1H) assay was conducted using the Matchmaker™ Gold Yeast One-Hybrid Library Screening System kit (Cat. no. 630491, Clontech).
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bioRxiv - Biochemistry 2020Quote: ... pOBD plasmids were then transformed into PJ69-4α yeast and were selected on tryptophan-dropout medium (Clontech, Mountain View CA, #630413); pGAD plasmids were transformed into PJ49-4a yeast and were selected on leucine-dropout medium (Clontech ...
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bioRxiv - Plant Biology 2022Quote: ... The recombinant constructs was transformed into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified ds CDNA was finally co-transformed with pGAD-T7 Rec into competent Y187 using Yeastmaker yeast transformation system (Takara, Clontech) and plated on SD-Leucine agar media ...