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Citations for Takara Bio :
1 - 50 of 2356 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... prey plasmids from library were rescued from yeast clones with “Easy Yeast Plasmid Isolation Kit” (Clontech) and sequenced ...
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bioRxiv - Microbiology 2023Quote: ... Yeast dual transformation and yeast mating was performed according to established protocols from Clontech. Yeast prey vectors were isolated from Y187 yeast and subsequently transformed into DH10B E ...
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bioRxiv - Plant Biology 2022Quote: ... All yeast media were purchased from Clontech.
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bioRxiv - Microbiology 2022Quote: The pBAD24a-based expression vectors of PG carboxypeptidases were constructed using In-Fusion cloning (Clontech, USA). The entire open reading frame of PG carboxypeptidase genes was amplified by PCR using cloning primer sets (See Table S2 ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids were extracted from yeast cells by Easy Yeast Plasmid Isolation Kit (#630467, Takara Bio) and transformed into E ...
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bioRxiv - Neuroscience 2020Quote: ... plasmids were rescued from yeast using the Easy Yeast Plasmid Isolation Kit (Clontech Takara Bio). Protein interactions were confirmed by co-transformation of the Nwd1 bait with each candidate prey plasmid into Y2H Gold yeast host cells ...
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bioRxiv - Neuroscience 2020Quote: ... plasmids were rescued from yeast using the Easy Yeast Plasmid Isolation Kit (Clontech Takara Bio). Protein interactions were confirmed by co-transformation of the Nwd1 bait with each candidate prey plasmid into Y2H Gold yeast host cells ...
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bioRxiv - Genetics 2022Quote: ... GenTLE (from yeast) high recovery kit (Takara Bio). The genomic DNA concentration was measured using the Quantifluor® ONE dsDNA Dye System (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... Yeast synthetic complete medium were purchased from Clontech. Taq DNA polymerase was from Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: Yeast Two-Hybrid (Y2H) Gold strain from Clontech (MATa ...
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bioRxiv - Plant Biology 2020Quote: ... Constructs were transformed into yeast strain AH109 following the small-scale LiAc yeast transformation procedure from the Yeast Protocol Handbook (Clontech, Mountain View, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... GenTLE (from Yeast) High Recovery Kit (9082, Takara Bio). The genomic DNA concentration was quantified using the Quantifluor® ONE dsDNA Dye System (E4971 ...
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bioRxiv - Cell Biology 2022Quote: ... The yeast two-hybrid assays were performed according to the Matchmarker Gold Yeast two-hybrid system manual from Clontech. Different variations of the constructs were co-transformed by the polyethylene glycol/ lithium acetate method into the AH109 yeast strain and selected on SD/-Leu-Trp plates ...
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bioRxiv - Bioengineering 2020Quote: ... Yeast transformation by Fast Yeast TransformationTM kit (Takara Bio) used for integrated linear pAUR101 vector and its associated genes in AUR1-C locus ...
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bioRxiv - Plant Biology 2021Quote: Yeast strain AH109 was transformed with bait (pGBKT7) and prey (pGADT7) constructs by following the small scale yeast transformation protocol from Yeastmaker™ Yeast Transformation System 2 (Clontech). Upon transformation ...
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bioRxiv - Molecular Biology 2020Quote: Yeast-2-hybrid assays were performed with the plasmids and strains from the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: Yeast media and plates were prepared according to recipes from Clontech and yeasts were grown at 30°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Matchmaker yeast two-hybrid library of arabidopsis cDNA purchased from Clontech. CD4-22 and CD4-10 libraries were converted from λ cDNA into plasmid libraries by transformation into BNN132 bacterial cells from which the plasmids were extracted ...
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bioRxiv - Bioengineering 2021Quote: ... Yeast transformation by Fast Yeast Transformation™ kit (Takara Bio) was used for integrated linear pAUR101 vector and its associated genes in the AUR1-C locus ...
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bioRxiv - Plant Biology 2024Quote: ... Yeast two-hybrid assays were performed using yeast strain AH109 (Clontech) following standard procedures (Yeast Protocols Handbook PT3024-1 ...
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bioRxiv - Plant Biology 2020Quote: ... This was then used to screen against the full yeast library via the yeast mating system following the manufacturer’s protocol (Matchmaker Gold Yeast Two-Hybrid System, Clontech). To further confirm the interaction between βC1 and AtPIFs ...
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bioRxiv - Genetics 2021Quote: Yeast Matchmaker Two-Hybrid System was obtained from Clontech (now Takara Bio), including pGBKT7 bait vector ...
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bioRxiv - Neuroscience 2022Quote: ... The yeast strains AH109 and Y187 as a partner (both from Clontech) were used.
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bioRxiv - Genetics 2021Quote: We followed procedures and used controls from the Matchmaker Gold Yeast Two-Hybrid System from Takara Bio ...
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bioRxiv - Developmental Biology 2020Quote: ... Yeast transformation was conducted with Yeast Transformation System 2 (Clontech NO.630439). All primers used were listed in supplementary Table 1.
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bioRxiv - Plant Biology 2022Quote: ... All yeast experiments were carried out using the Yeast Protocols Handbook (Clontech).
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bioRxiv - Plant Biology 2021Quote: ... Yeast (strain AH109) cotransformation was performed according to the Yeast Handbook (Clontech). An anti-HA (Roche ...
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bioRxiv - Microbiology 2023Quote: AH109 yeast cells (Clontech) were used for yeast two-hybrid experiments ...
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bioRxiv - Microbiology 2023Quote: ... Gold strain yeasts (Clontech) carrying plasmids expressing DB-fused NS5 sequences were co-transformed using a standard lithium/acetate procedure with 10 ng of linearized pPC86 empty vector (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... Yeast transformation was carried out by Fast Yeast Transformation ™kit (Takara Bio) to integrate linear pAUR101 vector and its associated genes in the AUR1-C locus ...
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bioRxiv - Plant Biology 2022Quote: The yeast strain Y2HGold and the Matchmaker Gold Yeast Two-Hybrid System (Clontech) were used for the yeast two-hybrid assay ...
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bioRxiv - Genetics 2023Quote: ... Yeast two-hybrid experiments were conducted using Matchmaker Yeast Two-Hybrid Kit (Clontech) following the product instruction ...
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bioRxiv - Plant Biology 2020Quote: Yeast two hybrid assay was performed using Matchmaker Gold Yeast Two-Hybrid System (Clontech). The OsSalT gene was cloned in-frame into the EcoRI and BamHI RE sites of the yeast vector ...
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bioRxiv - Plant Biology 2021Quote: All yeast protocols were performed according to the Yeast Protocols Handbook (Clontech, Mountainview, CA) with minor modifications ...
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bioRxiv - Plant Biology 2023Quote: All yeast work was performed according to the yeast protocols handbook (Clontech, Mountainview, CA). For Y2H screening ...
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bioRxiv - Plant Biology 2022Quote: Yeast one-hybrid assays were performed using the Matchmaker Yeast One-Hybrid System (Clontech). The DNA fragment 1,000 bp upstream of the start codon ATG of DDRM2 was cloned into pHis2.1 vector and SOG1 was cloned into the pGADT7 vector ...
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bioRxiv - Plant Biology 2024Quote: ... Yeast strain Y2H Gold (Clontech) was used for transformation ...
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bioRxiv - Evolutionary Biology 2024Quote: The AH109 yeast strain (Clontech) was used for all Y2H experiments ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid analysis was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: Yeast two-hybrid experiments were performed using the Matchmaker Gold Yeast Two-Hybrid System (Takara). The coding sequences were first cloned into the entry vector pDONR/Zeo (ThermoFisher) ...
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bioRxiv - Plant Biology 2021Quote: ... Yeast two hybrid assays were performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech). The standard protocol given in the kit was followed for Y2H assay ...
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bioRxiv - Cell Biology 2022Quote: Yeast two-hybrid analysis was performed with the Matchmaker Gold Yeast Two-Hybrid System (Takara). Y2H Gold and Y187 yeast strains were transformed with bait (pGBKT7 plasmids and derivatives ...
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bioRxiv - Plant Biology 2020Quote: ... Yeast transformation was performed according to the Yeastmaker Yeast Transformation System 2 User Manual (Clontech). The primers used are listed in Supplemental Table S2.
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bioRxiv - Plant Biology 2020Quote: ... Yeast transformation was performed according to the Yeastmaker Yeast Transformation System 2 User Manual (Clontech). More than 1 × 106 yeast clones were screened in synthetic defined (SD ...
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid screening was performed using the Matchmaker Gold Yeast Two-Hybrid System (Takara). The cDNA fragments corresponding to CEPDL2 and GrxS5 were subcloned into the pGBKT7 vector ...
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bioRxiv - Plant Biology 2024Quote: ... Yeast Two-Hybrid (Y2H) assays were carried out following the “Yeast Protocols Handbook” (Clontech Laboratories). Competent cells were transformed with 1 μg of DNA from each construct ...
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bioRxiv - Genetics 2022Quote: ... and then yeast two-hybrid assays were performed in a Gal4-based system from Clontech in a diploid strain by mating as previously described (Rossignol et al ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid assays were performed according to the Yeast Protocol Handbook (Clontech, catalog no. P3024). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: Yeast two-hybrid assays were performed according to the Yeastmaker Yeast Transformation System 2 manual (Clontech). The yeast strain AH109 was co- transformed with an AD-fused and a BD-fused construct using the lithium acetate method ...
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bioRxiv - Molecular Biology 2022Quote: Yeast two-hybrid screening was performed using the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio) as described by the manufacturer (Young ...