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Citations for Takara Bio :
101 - 150 of 2356 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: Yeast one-hybridization assay was performed using the Matchmaker® Gold Yeast One-Hybrid System (Clontech, Palo Alto, CA, USA). The promoter sequence (upstream 2kb from the start codon ...
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bioRxiv - Plant Biology 2021Quote: ... Confirmation of interaction was performed by targeted transformation of the specific constructs using the small-scale yeast transformation protocol as described in the yeast protocol handbook (Clontech). Transformants were grown in YPDA media to OD600 de 0,5 – 0,8 ...
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bioRxiv - Plant Biology 2020Quote: ... bait plasmids and prey plasmids were co-transformed into yeast strain AH109 according to the manufacturer’s protocol of GAL4-based yeast two-hybrid system (Clontech, USA). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant OmpA-pGBKT7 and Flg-pGBKT7 were finally transformed into Y2H Gold yeast strain separately using Yeastmaker yeast transformation system (Takara, Clontech).
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bioRxiv - Microbiology 2023Quote: A yeast screen for human cellular interacting proteins of pUL136 was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech) and the Mate and Plate Universal Human Library (Clontech) ...
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bioRxiv - Biochemistry 2023Quote: ... Constructs used to test protein-protein interactions were co-transformed into the yeast Saccharomyces cerevisiae strain AH109 using the Matchmaker Gold yeast two-hybrid system (Clontech). Empty pDEST-GBKT7 and pDEST-GADKT7 vectors were used as negative control ...
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bioRxiv - Molecular Biology 2022Quote: ... The freshly extracted plasmids were transformed into yeast strain Y2H gold by using a yeast transformation kit (TaKaRa, Beijing, China) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Bait and prey constructs were transformed into the haploid yeast strains Y2HGold and Y187 (Matchmaker Gold Yeast Two-Hybrid System, Clontech), respectively ...
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bioRxiv - Plant Biology 2024Quote: A yeast two-hybrid assay was conducted following the Clontech Yeast Two-Hybrid System protocol (Clontech, Mountain View, CA, USA). BGI1 was used as bait for screening a cDNA library constructed from Fielder (Li et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... The AD and BD fusion vectors were introduced into yeast (Saccharomyces cerevisiae) strain Y2H Gold using the polyethylene glycol/lithium acetate method described in the Yeast Protocols Handbook (Clontech). Co-transformed colonies were initially grown separately on SD/-Trp/-Leu plates and further screened for growth on SD/-Trp/-Leu/-His/-Ade plates ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids were used to transform competent yeast cells using YeastMaker Yeast Transformation System 2 (Takara, Mountain View, CA94043, USA), and the binding was validated in synthetic dropout-Leu-Trp-His-Ade medium supplemented with X-α-gal ...
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bioRxiv - Plant Biology 2021Quote: ... using Yeastmaker™ Yeast Transformation System 2 (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... as substrate as described (Yeast Protocols Handbook, Clontech).
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bioRxiv - Microbiology 2021Quote: ... using the Y2H Gold yeast reporter strain (Clontech). The Seasf1 ...
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bioRxiv - Plant Biology 2021Quote: ... performed according to the Yeast Protocols Handbook (Clontech).
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bioRxiv - Plant Biology 2023Quote: ... the Matchmaker Gold Yeast Two-Hybrid System (Clontech) was used ...
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bioRxiv - Plant Biology 2022Quote: The Matchmaker Gold Yeast 1-hybrid system (Clontech) with the Y1H gold yeast strain was used ...
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bioRxiv - Plant Biology 2022Quote: ... and introduced into the yeast strain AH109 (Clontech), which was then transformed with a pACT2 carrying cDNA expression library prepared from dark-grown Arabidopsis cell suspension (89) ...
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bioRxiv - Plant Biology 2022Quote: ... using Yeastmaker™ Yeast Transformation System 2 (Clontech). Transformants were grown on minimal synthetic defined (SD ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant OmpA-pGBKT7 and Flg-pGBKT7 were finally transformed into Y2H Gold yeast strain separately using Yeastmaker yeast transformation system (Takara, Clontech).
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bioRxiv - Microbiology 2022Quote: The yeast-two-hybrid library screening was performed according to the protocols of the Matchmaker® Gold Yeast Two-Hybrid System (TaKaRa). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... StNPR1 and StNPR374 were amplified and inserted into the pGBKT7 (bait) yeast expression vector through in vivo cloning with Matchmaker Gold Yeast Two-Hybrid System (Clontech, USA), to produce proteins with an N-terminal Gal4 DNA-binding domain ...
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bioRxiv - Plant Biology 2022Quote: ... Detection of EGT2 toxicity on yeast growth was performed according to Matchmaker® Gold Yeast Two-Hybrid System User Manual (Takara). Yeast cells expressing pGBKT7-EGT2 displayed a similar growth rate to yeast cells transformed with an empty pGBKT7 vector ...
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid tests were performed according to the Matchmaker® Gold Yeast Two-Hybrid System User Manual (Takara Bio Inc.). Briefly ...
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bioRxiv - Cell Biology 2020Quote: Yeast COPII components were cloned from the Saccharomyces cerevisiae S288c genome into appropriate expression vectors using In-Fusion (Takara), specifically ...
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bioRxiv - Plant Biology 2021Quote: ... SlGA2ox6 and SlGID1b-1 were constructed into pAbAi plasmid and transformed into Y1HGold yeast strain under the guidance of Yeastmaker™ Yeast Transformation System 2 (TAKARA, Japan). The recombinant yeast strain was confirmed by PCR conducted with Matchmaker™ Insert Check PCR Mix (TAKARA ...
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bioRxiv - Plant Biology 2021Quote: ... The yeast strain AH109 was used for the yeast two-hybrid assay according to MatchmakerTM Two-Hybrid System 3 (Clontech, Palo Alto) and grown on YPD (full medium ...
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bioRxiv - Plant Biology 2020Quote: ... while specific AD and BD recombinant plasmid pairs were used to co-transform yeast strain AH109 cells according to the Yeast Protocols Handbook (Takara, Dalian, China).
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bioRxiv - Microbiology 2021Quote: ... AH109 yeast cells (Clontech; Takara, Mountain View, CA, USA) were transformed with pGAL4-BD-Nmono using a standard lithium-acetate protocol ...
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bioRxiv - Microbiology 2021Quote: ... AH109 yeast cells (Clontech; Takara, Mountain View, CA, USA) were transformed with pGAL4-BD-Nmono using a standard lithium-acetate protocol ...
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bioRxiv - Microbiology 2022Quote: The Matchmaker® Gold Yeast Two-Hybrid System (TaKaRa) protocols were followed to verify the autoactivation of reporter genes by TaPHB-1 ...
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bioRxiv - Plant Biology 2020Quote: ... which were used to transform yeast strain Y2HGold (Clontech) using the lithium acetate method ...
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bioRxiv - Plant Biology 2021Quote: ... we used the GAL4 yeast two-hybrid system (Clontech). AP2 and WRKY16 were cloned into pGADT7-GW (Addgene Plasmid #61702 ...
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bioRxiv - Biochemistry 2020Quote: ... according to the Yeast Protocols Handbook (PT3024-1, Clontech).
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bioRxiv - Microbiology 2021Quote: ... AH109 yeast cells (Clontech; Takara, Mountain View, CA, USA) were transformed with this construct using a standard lithium-acetate protocol ...
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bioRxiv - Microbiology 2021Quote: ... AH109 yeast cells (Clontech; Takara, Mountain View, CA, USA) were transformed with this construct using a standard lithium-acetate protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Vectors were transformed into yeast strain Y2HGold (Clontech, Japan). Growth was determined as described in the Yeast Two-Hybrid System User Manual (Clontech ...
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bioRxiv - Molecular Biology 2020Quote: The Gal4-based yeast two-hybrid system (Clontech Laboratories) was used for testing the interaction ...
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bioRxiv - Plant Biology 2020Quote: ... The MatchMaker yeast two-hybrid system (Takara, Dalian, China) was used to evaluate the interactions among TaHsp70-30b ...
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bioRxiv - Plant Biology 2024Quote: ... following standard procedures (Yeast Protocols Handbook PT3024-1, Clontech). The proteins were extracted by post-alkaline methods59 and detected by western blot with antibody Myc (Abmart ...
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bioRxiv - Molecular Biology 2022Quote: ... Vectors were transformed into yeast strain Y2HGold (Clontech, Japan). Yeast transformation was performed according to the Yeastmaker Yeast Transformation System 2 User Manual (Clontech ...
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bioRxiv - Microbiology 2024Quote: ... Yeast clones containing pGBKT7-53 and pGADT7-T (Clontech) were used as positive controls ...
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bioRxiv - Cell Biology 2024Quote: ... were transformed into the PJ69-4A yeast strain (Takara). To monitor growth on selective dropout plates ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids were extracted from all the colonies which grew on QDO/X/A agar plate using Easy Yeast Plasmid Isolation Kit (TaKaRa). PCR was carried out using the extracted plasmids as template ...
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bioRxiv - Cell Biology 2020Quote: ... Cotransformation with pGBT9 and pGADT7 plasmids was performed by a modification of the lithium acetate procedure as described in the Yeast Protocols Handbook from Clontech. Plasmids expressing GAL4-activation domains and GAL4-DNA-binding domains contain the Leu2 and Trp1 selection markers ...
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bioRxiv - Immunology 2021Quote: Variable heavy chain and light chain sequences were PCR amplified from yeast plasmid DNA and cloned into CMV/R IgG expression vectors using InFusion (Takara). Plasmid DNA for mammalian expression of the His-tagged SARS-CoV-2 RBD in a pCAGGS vector was kindly provided by Dr ...
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bioRxiv - Immunology 2022Quote: ... Mate & Plate™ cDNA Library in a pGADT7 vector with the GAL4 DNA-activation domain (AD) and pre-transformed into yeast strain Y187 was acquired from Takara Bio ...
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bioRxiv - Immunology 2022Quote: Yeast two-hybrid screening was performed using a GAL4-based system (Matchmaker Gold Yeast Two-Hybrid System; Takara Bio, Mountain View, CA, USA). Assays and screenings were performed according to the manufacturer’s provided user manual ...
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bioRxiv - Cell Biology 2022Quote: ... while pGADT7-CmVPS41s and pGADT7-T control prey plasmids were transformed into yeast strain Y187 following the Yeastmaker Yeast Transformation System 2 instructions (Clontech by Takara Bio, Mountain View, USA). The transformed cells were grown either in SD-Trp agar plates for Y2HGold or in SD-Leu agar plates for Y187 at 30 ºC for 3-5 days ...
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bioRxiv - Plant Biology 2020Quote: The pBridge yeast three-hybrid system (Clontech CATALOG No. 630404) was used to test if the wheat VRT2 protein can interfere with the interactions between SEPALLATA and SQUAMOSA proteins ...