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Citations for Takara Bio :
1951 - 2000 of 3733 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: Polymerase chain reaction (PCR) amplification of DNA receivers for plasmids construction and colony PCR were performed using PrimeSTAR® Max DNA Polymerase (R045B, TaKaRa, Kyoto, Japan). PCR primers were synthesized by Integrated DNA Technologies (IDT ...
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bioRxiv - Plant Biology 2019Quote: ... One microgram of DNA-free RNA was used to synthesize cDNA by using PrimeScriptTM RT Reagent Kit (TAKARA, Kusatsu, Shiga, Japan).
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bioRxiv - Developmental Biology 2021Quote: ... and reverse-transcription (RT) was performed with 1.5 µg of total RNA and PrimeScript reverse transcriptase (Takara Bio Inc, Kusatsu, Japan). RT-quantitative (q)-PCR was performed on a CFX96 C1000 real-time PCR cycler (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from DRG was extracted using RNeasy micro kit and reverse-transcribed using PrimeScript RT Reagent Kit (Cat# RR037A, TaKaRa, Japan). Real-time PCR was performed using the SYBR Premix Ex Taq™ (Cat# DRR041A ...
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bioRxiv - Cell Biology 2019Quote: ... and PrimeScript RT reagent kit with gDNA Eraser and SYBR Premix Ex Taq II (T1i RNase H Plus) (TaKaRa, Otsu, Japan) were utilized to carry out quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted using a Plant RNA Kit (R6827, Omega) and then reverse transcribed using PrimeScript™ RT Master Mix (RR036Q, Takara) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Equal quantities of RNA (1µg) were adopted for the synthesis of total cDNA using the PrimeScriptTM RT reagent kit with gDNA Eraser (TaKaRa Dalian, China). The transcript levels of CCR1 ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were treated for 1 h by DNase I at 37°C and reverse-transcribed using a Prime ScriptTM RT reagent kit with gDNA Eraser (Takara, Japan). Quantitative PCR was performed as mentioned above ...
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bioRxiv - Plant Biology 2021Quote: ... followed by reverse transcription using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio, Shiga, Japan). Quantitative PCR was conducted using the KAPA SYBR FAST qPCR Master Mix (2X ...
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bioRxiv - Plant Biology 2020Quote: ... was used to generate templates to synthesize first chain cDNA using the PrimeScript™ RT Master Mix kit (TAKARA, Dalian, China), with three biological replicates sample ...
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng of total RNA was used for the cDNA synthesis reaction using PrimeScript™ RT Master Mix kit (TaKaRa RR036A) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of total RNA from each condition was converted into cDNA using PrimeScript RT reagent with gDNA Eraser kit (Takara Clontech) and MJ Research PTC-220 Dyad PCR System (Conquer Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... An aliquot of 1 μg of the total RNA was used to synthesize cDNA using PrimeScript™ RT reagent Kit with gDNA eraser (Takara). qRT-PCR analysis was performed on a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Total RNA was then subjected to genomic DNA (gDNA) elimination and reverse-transcription according to PrimeScript™ RT reagent kit protocols (Takara). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... . cDNA was synthesized by the use of PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the reverse transcription protocol (Takara). The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex TaqTM kit (TaKaRa ...
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bioRxiv - Plant Biology 2022Quote: ... The 1 μg total RNAs were used to synthesize cDNA with the PrimeScript™RT reagent Kit with gDNA Eraser (Takara). qRT-PCR was carried out with SYBR Premix Ex Taq II Premix (Takara ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized by the use of PrimeScript™RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the reverse transcription protocol (Takara). The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex Taq™ kit (TaKaRa ...
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bioRxiv - Neuroscience 2020Quote: ... The first strand of cDNA was synthesized by reverse transcription of the mRNA via a 1hr incubation at 42°C by adding the following to the reaction mixture above: 1µl SMARTScribe™ RT (Takara, #639538), 1µl dNTPs 10mM (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... total RNA (1 μg) was reverse-transcribed into cDNA using the PrimeScript RT reagent Kit with gDNA Eraser (Takara, Shiga, Japan), and relative mRNA expression was determined by real-time PCR using FastStart Universal SYBR Green Master [Rox] (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The copy number of viral RNA was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, cat# RC300A). The fluorescent signal was acquired using a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... and the reverse transcription products were amplified using the Agilent-Strata gene MxReal-Time qPCR system with a PrimeScript™ RT Reagent Kit (Takara). Reverse transcription products were amplified using an Agilent-Strata gene MxReal-Time qPCR system with TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of RNA samples (200 ng) were used to generate complementary DNA using a PrimeScript RT reagent kit (TaKaRa Bio) with oligo-dT and random primers ...
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bioRxiv - Neuroscience 2019Quote: ... 1 μg total RNA was reverse transcribed into cDNA using the PrimeScriptTM RT reagent kit with gDNA Eraser (Perfect Real Time) (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: RNA was extracted using the Biostic Blood Total RNA Isolation Kit sample (MO BIO laboratories, Inc) and cDNA was obtained using the PrimeScript™RT reagent Kit (Takara). RTqPCR gene expression analysis was performed using the Taqman probes HSF2-Hs00988309_g1 and GADPH-Hs02758991_g1 for normalization (Applied Biosystems).
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bioRxiv - Microbiology 2019Quote: ... The bacterial RNA was reverse transcribed into complementary DNA (cDNA) using the PrimeScript RT reagent Kit (Perfect Real Time) (Takara: RR037A) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... About 1 μL of viral RNAs were used as template to synthesize cDNAs with PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa). Absolute quantitative real-time PCR assay was performed with SYBR green I (TOYOBO ...
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bioRxiv - Cell Biology 2019Quote: ... from each ample was used to synthesize first-strand cDNA by reverse transcription using the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and reverse transcription was further performed with 500 ng total RNA as initiation material with PrimeScriptTM RT Master Mix (TaKaRa, RR036A). qRT-PCR was conducted with 2 x S6 Universal SYBR qPCR Mix (NovaBio ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was reverse-transcribed from 800 ng of total RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara). Gene-specific primers with appropriate amplification efficiency (0.95–1.05 ...
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bioRxiv - Genetics 2019Quote: ... The total amount of DNA-free RNA obtained from each tissue was reverse transcribed using the PrimeScript RT reagent Kit (TaKaRa, Japan) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was reverse transcribed using PrimeScriptTM RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the manufacturer’s protocol (Takara, Kusatsu, Japan). DNA and cDNA samples were subjected to 16S rRNA gene amplicon sequencing ...
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bioRxiv - Physiology 2021Quote: ... First-strand cDNA was synthesized from total RNA using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Shiga, Japan).
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bioRxiv - Microbiology 2021Quote: ... The copy number of viral RNA was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). The fluorescent signal was acquired using a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The cDNAs were synthesized from 1 μg total RNA using the Prime Script™ RT reagent kit (Perfect Real Time; Takara). All qRT–PCR (quantitative reverse transcription–PCR ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was extracted directly from the virus stock using the Indimag Pathogen kit (Indical Biosciences) and transcribed to cDNA using the PrimeScript™ RT reagent Kit (Takara) using oligo-dT and random hexamers ...
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bioRxiv - Genomics 2021Quote: ... and reversely transcribed into cDNA according to the PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa, Japan) manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA was synthesized according to the manufacturer’s instruction of the PrimeScriptTM RT reagent Kit with the genomic DNA Eraser (Takara, Kyoto, Japan). Then ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... according to the manufacturer’s instructions and reverse transcribed using the PrimeScript RT Reagent Kit with the gDNA Eraser (Takara, Shiga, Japan). To amplify cDNA ...
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bioRxiv - Genomics 2022Quote: The expression of each erbb3b gene and the two housekeeping genes (rpl7 and rpl32) was obtained by RT-qPCR at 65 °C (PrimeScript transcriptase, Takara, SYBRgreen) and normalized with the Pfaffle equation (Ståhlberg et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was reverse transcribed into 10 μL of complementary DNA (cDNA) with PrimeScriptTM RT Master Mix (Takara Biomedical Technology, China) at 37°C for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized using the PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa Bio Inc., Dalian, China) from 1 μg total RNA in a final volume of 20 μL according to the provided protocol ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used to synthesize first-strand cDNA with PrimeScript RT enzyme together with gDNA eraser (Takara, Japan). qRT□PCR was performed on a Roche LightCycler 480 (Roche Diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... total RNA was cleaned by genomic removal and then reversely transcribed (Prime Script RT reagent Kit with gDNA Eraser, Takara, China). The rRNA removal ...
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bioRxiv - Genetics 2022Quote: ... An aliquot of 1 μg of the total RNA was used to synthesize cDNA using PrimeScript™ RT reagent Kit with gDNA eraser (Takara). qRT-PCR analysis was performed on a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Systems Biology 2023Quote: ... 500 ng of isolated RNA was then used as input for generating cDNA and cDNA synthesis was performed using the PrimeScript RT kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... a complete cDNA sequence of bovine hnRNPM (NM_001191223) was obtained from genomic DNA of MDBK cells using PrimeScript RT reagent Kit (TaKaRa, Shiga, Japan), followed by a PCR reaction using PrimeSTAR Max DNA Polymerase (TaKaRa ...
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bioRxiv - Developmental Biology 2023Quote: ... was eliminated by digesting with genomic DNA eraser buffer and cDNA was obtained by reverse transcription of RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara, RR047A). To measure the values of genes specifc primers ...
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bioRxiv - Molecular Biology 2023Quote: ... and then the isolated RNA (1 μg) was reverse transcribed into cDNA (20 μL) using PrimeScript™ RT Master Mix (Perfect Real Time, Takara). The qPCR reaction was initiated at 95 °C for 3 min ...
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bioRxiv - Cell Biology 2023Quote: ... An amount of 1 μg RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (Takara Biomedical Technology, China). For RT-qPCR ...
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bioRxiv - Zoology 2023Quote: ... First- strand cDNA was synthesized from total RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Shiga, Japan). PCR amplifications were performed using the KOD One PCR Master Mix (TOYOBO ...