Labshake search
Citations for Takara Bio :
2151 - 2200 of 3947 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... which was then reverse-transcribed into first-strand cDNA using the Prime-Script-TM RT Reagent Kit (RR036, Takara, Kyoto, Japan). The quantitative real-time polymerase chain reaction was performed using the ABI 7300 sequencer and SYBR Premix Ex Taq-TM (RR420 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) using the RR036A PrimeScript™ RT Reagent Kit (Perfect Real Time) supplied by TaKaRa. The amplification products were quantified using SYBR Green (Vazyme) ...
-
bioRxiv - Genomics 2023Quote: ... The same amount of input RNA for each batch was used for reverse transcription (RT) reactions with SMARTScribe™ Reverse Transcriptase (Takara) and oligo dT following the manufacturer’s manual ...
-
bioRxiv - Genetics 2024Quote: ... The extracted RNA was quantified by Nanodrop and 500 ng of it was converted into cDNA using a PrimeScript™ RT reagent Kit (Takara). Quantitative RT-PCR (qRT-PCR ...
-
bioRxiv - Cancer Biology 2024Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) following the instructions of PrimeScript RT reagent kit (Takara, Beijing, China). qRT-PCR was performed using the AceQ Universal SYBR qPCR Master Mix (Vazyme) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Residual genomic and plasmidic DNA was removed using DNA-free kit (InvitrogenTM) and 500 ng of RNA were retro-transcribed with PrimeScript RT Master Mix (Takara Bio) following manufacturer instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... and an equal volume of RNA from the other time points for each condition were retrotranscribed with PrimeScript RT Master Mix (Takara Bio) to account for minimal cell death during RNase incubation ...
-
bioRxiv - Pathology 2024Quote: Expression for IFN-related genes was conducted by reverse transcription using the PrimeScript RT Reagent Kit (Perfect RealTime, Takara Bio Inc.). qPCR reaction was performed in duplicate using Takyon ROX SYBR MasterMix blue dTTP (Eurogentec ...
-
bioRxiv - Microbiology 2024Quote: ... the 2nd PCR product was amplified from 10 ng purified 1st PCR product using the TaKaRa Ex Taq HS (TaKaRa Bio Inc., Japan; #RR006A) with a forward (5’-AATGATACGGCGACCACCGAGATCTACAC-Index2-ACACTCTTTCCCTACACGACGC ...
-
bioRxiv - Plant Biology 2020Quote: ... were amplified by PCR using PrimeSTAR GXL DNA polymerase (Takara) and gene-specific primer sets ...
-
bioRxiv - Cell Biology 2020Quote: ... sgRNA inserts were PCR amplified using ExTaq DNA Polymerase (Takara). The resultant PCR products were purified and sequenced on a HiSeq 2500 (Illumina ...
-
bioRxiv - Cell Biology 2020Quote: ... PCR was performed using SYBR Green Mix (Takara Bio, Japan). The primer sequences were shown in Table 1.
-
bioRxiv - Genetics 2021Quote: ... Colony PCR was performed using Takara Taq polymerase (Clontech, TAKR001C). Plasmid DNA was isolated from cultured bacteria using QIAprep Spin Miniprep Kit (Qiagen ...
-
bioRxiv - Developmental Biology 2020Quote: ... qRT-PCR was performed via SYBR Premix Ex Taq (Takara) and Rotor-Gene 3000 system (Corbett Research) ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR reaction consisted of PrimeSTAR Max Premix 1x (Takara Bio), 0.4 μM of each primer and 30-100 ng DNA template ...
-
bioRxiv - Molecular Biology 2020Quote: ... PCR was conducted using PrimeSTAR Max DNA Polymerase from TaKaRa. The PCR products were checked by agarose gel electrophoresis and inserted into pET28a plasmid between the BamHI and EcoRI sites with Gibson Assembly method ...
-
bioRxiv - Immunology 2019Quote: ... The RACE PCR products were cloned into pMD-19Tvector (Takara) and sequenced.
-
bioRxiv - Neuroscience 2019Quote: ... The PCR product was InFusion-cloned (Clontech, catalogue number 639645) into the pBAC-ECFP-15xQUAS-TATA-SV40 plasmid7 (Addgene #104875) ...
-
bioRxiv - Neuroscience 2020Quote: ... and then amplified by PCR using PrimeStar HS polymerase (Takara). Primers for amplifying the prenyl-Cdc42 coding sequence and 3’UTR were engineered to contain terminal Not I and Sal I restriction sites ...
-
bioRxiv - Immunology 2021Quote: ... a semi-nested PCR was performed using PrimeStar MAX (Takara) for 35 cycles ...
-
bioRxiv - Immunology 2021Quote: ... PCR was performed for 35 cycles using PrimeStar MAX (Takara). Primers used for these reactions can be found in Supplementary Table 1 ...
-
bioRxiv - Cell Biology 2020Quote: ... PCR products were sub-cloned in pEGFP expression vectors (Clontech). KIF13B was amplified by PCR from GFP-KIF13B and inserted into a Bio-mCherry-C1 vector to make mCherry-KIF13B ...
-
bioRxiv - Neuroscience 2021Quote: ... Viral titers were assayed by Lenti-X qRT-PCR (Clontech) and titers were adjusted to 1×108 copies/ml before injection.
-
bioRxiv - Plant Biology 2021Quote: ... PCR amplified products (PrimeSTAR GXL DNA Polymerase, TaKaRa Bio Inc.) using primers and DNA templates listed in Table S4 were cloned into pLRE::LRE-cYFP plasmid linearized with SpeI-HF and AscI by using the In-Fusion HD Cloning Plus system (Clontech) ...
-
bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed with 2x SYBR mix (Takara) in a QuantStudio-6 Flex Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2020Quote: ... then amplified by PCR with PrimeSTAR pre-mix HS (Takara), and cloned into pCR4Blunt-TOPO (ThermoFisher) ...
-
bioRxiv - Cell Biology 2020Quote: ... PCR fragments were annealing in 1X prime star buffer (Takara) and Surveyor enzyme was added to digest the annealed DNA fragments at 42 °C for 1h ...
-
bioRxiv - Microbiology 2022Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio) per manufacturer’s instruction ...
-
bioRxiv - Molecular Biology 2020Quote: ... T4 ligase and KOD PCR kit were purchased from Takara Bio ...
-
bioRxiv - Neuroscience 2020Quote: A multiple-step overlap-extension PCR (Pyrobest Polymerase, Takara Bio) was used for site-directed mutagenesis and construction of chimeric protein constructs ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR products were cloned into a pGEX6P2 vector (Clontech) which was subcloned the GFP gene at the N-terminus 48 ...
-
bioRxiv - Microbiology 2022Quote: ... the GFP fragment was PCR amplified from pEGFP-N1 (Clontech) using specific primers to insert MluI/NotI restriction enzyme sites and ligated on those restriction enzyme sites into pCI-Neo (Promega) ...
-
bioRxiv - Microbiology 2019Quote: ... digesting the PCR fragment with SacI (TaKaRa Bio Inc., Japan) and ligating again with Ligation high ver ...
-
bioRxiv - Microbiology 2020Quote: ... PCR products were cloned using the In-Fusion method (Clontech) into pGB135 digested with BamHI and EcoRI to generate plasmids pJRP33-34 ...
-
bioRxiv - Microbiology 2020Quote: ... PCR products were cloned using the In-Fusion method (Clontech) into pAMD-BA-lacZ digested with NheI and SphI to generate plasmids pJRP54-72 (see Table 2 for details).
-
bioRxiv - Biochemistry 2019Quote: ... All DNA was cloned using backbone PCR and inFusion (Clontech). pHR vectors were used for mammalian cell experiments and pBAD vectors were used for bacterial protein overexpression ...
-
bioRxiv - Neuroscience 2019Quote: ... Amplification was performed using Advantage GC 2 PCR kit (Clontech) and PCR products were cloned and sequenced.
-
bioRxiv - Biochemistry 2019Quote: ... PCR was performed with PrimeSTAR® max DNA polymerase (Takara) and Ex Taq® polymerase (Takara) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The 5’-RACE PCR was performed with ExTaq polymerase (TaKaRa) using the following primers ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The 3’-RACE PCR was performed with ExTaq polymerase (TaKaRa) using the following primers ...
-
bioRxiv - Developmental Biology 2021Quote: ... Real-time PCR was performed using SyBr green mix (Takara) in Realtime PCR machine (BioRad CFX96).
-
bioRxiv - Cell Biology 2020Quote: ... CloneAmp HiFi PCR Premix (Takara, Mountain View, USA, Catalog #639298) was used to PCR amplify the PLIN2 cDNA and gel purified via NucleoSpin Gel and PCR Clean Up (Takara ...
-
bioRxiv - Microbiology 2021Quote: ... PCR amplification was performed using the CloneAmp HiFi Premix (Takara) and the primers listed in Table S4 under the following conditions ...
-
bioRxiv - Microbiology 2020Quote: ... The restricted backbone and PCR products were InFusion (Takara Bio) ligated ...
-
bioRxiv - Plant Biology 2021Quote: ... the promoter regions were amplified by PCR (PrimeSTAR max, Takara) and cloned into pGEM/T-EASY containing LUC+ for RSL4 and LRL3 ...
-
bioRxiv - Genetics 2020Quote: ... Amplification was performed on the Takara PCR Thermal Cycler (Takara) under the following conditions ...
-
bioRxiv - Genetics 2020Quote: ... Reactions were performed in the Takara PCR Thermal Cycler (Takara) with 25 cycles of denaturation at 96 °C for 10 s and annealing and elongation at 60 °C for 30 s ...
-
bioRxiv - Immunology 2022Quote: ... A high-fidelity Advantage HF2 PCR kit (Takara, Cat#639123) was used in each of the PCR steps involved in preparing the sequencing libraries ...