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Citations for Takara Bio :
2201 - 2250 of 3947 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... ORF3a was amplified with Takara PrimerSTAR PCR enzyme (R050B, Takara) using the following primers:
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bioRxiv - Molecular Biology 2022Quote: ... PCR was performed using PrimeSTAR Max DNA polymerase (Takara, Dalian) to amplify the predicted CYP9A genes under the following cycling conditions ...
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bioRxiv - Biochemistry 2022Quote: ... All PCRs were performed using PrimeSTAR Max DNA Polymerase (Takara) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... EmeraldAmp GT PCR Master Mix (Takara Bio USA, Cat# RR310A) and gene specific primers (Supplementary Table 7 ...
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bioRxiv - Developmental Biology 2022Quote: ... Candidate enhancers were PCR amplified (Takara PrimeSTAR GXL premix, R051A) from mouse genomic DNA using primers listed below ...
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bioRxiv - Microbiology 2022Quote: ... PCR was performed using PrimeSTAR Max DNA Polymerase (Takara Bio) and the pNEN142-agtA plasmid as a template ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were pooled by template and purified (Nucleospin, Takara). RACE amplicons were end repaired ...
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bioRxiv - Microbiology 2022Quote: ... Genotyping was performed using Terra PCR Direct Polymerase Mix (Takara) following manufacturers specifications.
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bioRxiv - Immunology 2023Quote: ... quantitative PCR was performed using SYBR Green mixture (Takara Bio). The average mRNA fold changes were calculated by 2-ΔΔCT ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR amplification was performed by Prime Star DNA polymerase (Takara). The GFP and rGFP were cloned into the multiple cloning site (MCS ...
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bioRxiv - Microbiology 2024Quote: ... Genotyping was performed using Terra PCR Direct Polymerase Mix (Takara) following the manufacturer’s specifications ...
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bioRxiv - Developmental Biology 2024Quote: ... linker italics) were used to PCR amplify (Takara PrimeStar Max) the homology repair template (HRT) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR mixture containing 10 µL Emerald Master mix (2X) (TaKaRa), 2 µL total genomic DNA (10-15 ng/µL) ...
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bioRxiv - Molecular Biology 2023Quote: ... purified using NucleoSpin Gel and PCR Clean-Up Kit (Takara), cloned into pRACE vector (provided in the kit ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR was performed using SYBR green (Takara, Kyoto, Japan) to measure mRNA levels of Adam11.L and GAPDH was used to normalize total cDNA amount ...
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VapC12 ribonuclease toxin modulates host immune response during Mycobacterium tuberculosis infectionbioRxiv - Microbiology 2023Quote: ... Real-time PCR was performed with 2 × SYBR mix (TaKaRa) in a QuantStudio-6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... and the SYBR Green Real-time PCR Master Mix (Takara). Applied Biosystems™ 7500 Real-Time PCR Systems (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... using PCR and cloned in the pEGFP-N1 vector (Clontech) at the KpnI and NotI restriction sites ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting PCR products were assembled using Infusion cloning (Takara) to create the pMal-Strep-WEEP.
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bioRxiv - Bioengineering 2023Quote: PCR reactions were performed using PrimeSTAR HS DNA Polymerase (Clontech) or Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were run on a Smart Cycler System (Takara). Ct values of the gene targets were normalized to GAPDH ...
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bioRxiv - Cell Biology 2023Quote: ... the EGFP sequence was PCR amplified from pEGFP-N1 (Clontech), and cloned in the pAIB-CAG lentiviral vector (a gift from Claudio Ballabio ...
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bioRxiv - Genetics 2023Quote: DNA amplification was performed using PCR with PrimeSTAR GXL (Takara) using standard protocols and 35 cycles unless otherwise specified ...
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bioRxiv - Plant Biology 2023Quote: ... and standard PCR was performed using ExTaq HS kit (TaKaRa). Control reactions were run using wild-type DNA as a template.
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bioRxiv - Genomics 2023Quote: ... The second PCR was performed using PrimeSTAR GXL polymerase (Takara) with 5 ng of purified product from the previous PCR was used as a template (50 μL/reaction) ...
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bioRxiv - Genomics 2023Quote: ... The first PCR was performed using PrimeSTAR GXL polymerase (Takara) and 1:2000 SYBR Green I (20 μL/reaction ...
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bioRxiv - Bioengineering 2023Quote: ... PCR was performed with PrimeSTAR® DNA Polymerase (Takara Bio) and the following primers Rosa26-F (5’-GGGAGTTCTCTGCTGCCT-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... by PCR using a PrimeSTAR Mutagenesis Basal Kit (Takara, R046A) and the following primers ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR amplification was done using ExTaqTM DNA polymerase (Takara, Japan). The PCR product ...
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bioRxiv - Biochemistry 2024Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio). The sequences of all primers in this study can be found in Table S3 ...
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bioRxiv - Neuroscience 2024Quote: ... and the Advantage 2 PCR kit (Takara/Clontech Cat # 639207). Libraries were checked for quality and approximate concentration on a 1% agarose gel.
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bioRxiv - Neuroscience 2024Quote: ... and the Advantage 2 PCR kit (Takara/Clontech Cat # 639207). Libraries were checked for quality and approximate concentration on a 1% agarose gel.
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bioRxiv - Biochemistry 2024Quote: ... This PCR was performed by PrimeSTAR Max polymerase (Takara Bio) with the following settings ...
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bioRxiv - Microbiology 2024Quote: ... Conventional PCR was performed with TaKaRa EX Taq kit (TaKaRa) and included 5 µL 10X PCR Buffer (Mg2+ plus) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR amplification was performed using PrimeSTAR GXL DNA polymerase (TaKaRa). The resulting barcoded PCR products were pooled for SMRTbell library construction and sequenced on the Sequel platform.
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bioRxiv - Cell Biology 2024Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The cDNAs were used for PCR with rTaq polymerase (TaKaRa). The primer sequences used for amplification are listed in Table S1.
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bioRxiv - Cancer Biology 2023Quote: ... sublines 14D7 and 24C7.8 The presence of the deletion on the translocated and the wildtype allele was validated by PCR using the Terra PCR Direct Polymerase Mix (Takara Bio Europe; supplemental Table 7). From line 14D7 ...
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bioRxiv - Evolutionary Biology 2021Quote: NifS genes (Pelo6206 and Pelo13211) were amplified from cDNA using specific primers (S4 Table) and PrimeSTAR® Max DNA Polymerase (Takara Bio Inc.) premix ...
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bioRxiv - Microbiology 2019Quote: ... The cDNA samples were subjected to quantification by real-time PCR with specific primers F (5’-GATTACCAGGGATTTCAGTCG-3’) and R (5’-GACACCTTTAGGCAGACCAG-3’) using SYBR ® Premix Ex TaqTM II (Tli RNaseH Plus) (TaKaRa Cat: RR820A). Fold change in RNA was calculated by double-standard curve methods and β-actin as an internal control.
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bioRxiv - Biochemistry 2020Quote: ... RAD51 was PCR amplified from pGAT3-RAD51 (Davies et al. 2007) with primers that included Mfel and XhoI sites and then ligated into pGADT7 (Clontech, Mountain View, CA) digested with EcoRI/XhoI ...
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bioRxiv - Microbiology 2020Quote: pEX18Tc-hpdA was constructed for gene knockout by fusing the erythromycin resistance gene and two upstream and downstream fragments of the target gene amplified with the primers shown in Table 3 to Sac I/Hind III-digested pEX18Tc with the In-Fusion® HD Cloning Kit (TaKaRa, Dalian, China). The resulting plasmid pEX18Tc-hpdA was transformed into E ...
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bioRxiv - Microbiology 2020Quote: The coding sequence for MrvR was PCR amplified from 10/84 genomic DNA using primers optimized for subsequent cloning into the four supplied BIC Brevibacillus expression system pBIC plasmids (Takara cat. # HB300/HB310) in order to generate secreted ...
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bioRxiv - Plant Biology 2019Quote: ... 1.5 kb) was amplified with P1 and P2 primers (see Supplementary Table 1) from Col genomic DNA using PrimeStarMax (Takara Bio, Kusatsu, Japan) and inserted in HindIII-XbaI digested pGWB51131 by the SLiCE method32 to give p511G1pro ...
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... Mutations of MavH were introduced by in vitro site-directed mutagenesis using specific primers containing the defined base changes and PrimeSTAR® Max DNA Polymerase (Takara Bio, Inc.) premix ...
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bioRxiv - Plant Biology 2022Quote: ... the LNK2 promoter was cloned from just before the start of the upstream gene through 142 bases into exon 4 from genomic DNA using primers pDAN1018 and pDAN1019 (Table S2) and inserted into pB7-HFC PmeI/BglII digest and In-Fusion HD cloning (Clontech, Mountain View, California) to generate pB7-LNK2p::LNK2-HFC ...
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bioRxiv - Plant Biology 2022Quote: ... 2098 bp of the TOC1 promoter was cloned using primers pDAN2735/pDAN2736 (Table S2) and inserted via In-Fusion HD cloning (Clontech, Mountain View, California) into the pGreenII 0800-LUC plasmid (Hellens et al. ...
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bioRxiv - Plant Biology 2022Quote: ... RVE8 was cloned from genomic DNA without the stop codon using primers pDAN1127 and pDAN1128 (Table S2) and cloned into NotI/AscI-digested pENTR-MCS through In-Fusion HD cloning (Clontech, Mountain View, California). pENTR-RVE8-no stop was then recombined using LR Clonase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... the gene of interest was cloned from its pENTR-STOP template using primers pDAN2349/pDAN2350 (Table S2) and recombined into pGADT7 digested with EcoRI using In-Fusion HD cloning (Clontech, Mountain View, California). For cloning into pGBKT7 ...
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bioRxiv - Genetics 2024Quote: Two μg of total RNA was reverse transcribed into cDNA templates using RNA to cDNA EcoDry™ Premix kit including both random hexamer and oligo(dT)18 primers (Takara Bio, 639548). KAPA SYBR® FAST qPCR Master Mix (2X ...