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Citations for Takara Bio :
1551 - 1600 of 3733 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... acaule was converted into cDNA with ReverTra Ace (TOYOBO, Osaka, Japan) using a random primer (TAKARA BIO, Kusatsu, Japan). cDNA diluted 10-fold with water was used as a template for PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... The cDNA was amplified with gene-specific primers (Supplemental Table 1) and SYBR Premix Ex Taq II kit (TaKaRa). Data were analyzed using a 2−ΔΔCt method.
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bioRxiv - Cell Biology 2019Quote: ... Using the online In-Fusion™ HD (IF) cloning system primer design website (Takara Bio USA, Mountain View, CA), we made primer sets for TMEM163 SNP ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A plasmid encoding the G protein chimera GqGi3 bearing the core of human Gαq and the last 4 amino acid of Gαi3 was generated by primer mutagenesis and In-Fusion HD Cloning (Clontech) in a pcDNA3.1 vector ...
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bioRxiv - Microbiology 2020Quote: ... 1) were annealed to a complementary 20-nt primer containing a 5’-fluorescein label (5’FAM-GUCAUUCUCCUAAGAAGCUA-3’, Takara). To perform the primer extension assay ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and ii) because the relevant primer sequence is proprietary (both confirmed by Takara EU tech support in November 2020), so that we initially did not have access to its sequence ...
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bioRxiv - Genomics 2021Quote: ... The purified poly(A)+ RNA was reverse-transcribed with oligo(dT) primer and reverse transcriptase XL (AMV) (Takara Bio).
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bioRxiv - Cell Biology 2021Quote: ... of PINK1 or OMA1 encoding plasmid using appropriate primers followed by Gibson assembly (In-Fusion HD Cloning system, Clontech) into the SalI-XhoI of the pLenti-CMV-Neo vector ...
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bioRxiv - Microbiology 2022Quote: ... Then 50 nl of primer pair solution mixed with 1X SmartChip TB Green Gene Expression Master Mix (Takara Bio) were loaded onto the SmartChip ...
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bioRxiv - Microbiology 2022Quote: ... using specific primers flanked by 15bp overlapping regions from pL6-var2csa-promoter-deletion plasmid and pUF1_Cas9 (list of primers, see Supplementary Table S8) that allowed the cloning by infusion cloning (Clontech, Takara Bio USA ...
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bioRxiv - Neuroscience 2023Quote: ... the insert was amplified using primers (Key Resources Table) designed with the In-Fusion Cloning Tool (Takara-bio Inc.). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... Technical duplicates were performed with thirty-five cycles of PCR in a 50 μL reaction with 0.4 mM TprK forward and reverse primers (Table S4) using the 2x CloneAmp MasterMix (Takara) with a 62°C annealing temperature ...
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bioRxiv - Microbiology 2022Quote: ... using the primers listed in S-Table 1B into pcDNA3.1-P2A-tagRFP by the In-Fusion HD Cloning Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... using Psme1Sal1-F/Psme1BamHI-R primer pairs (Table S4) and cloned as a Sal1/BamH1 fragment into pEGFPC1 (Clontech). For production of GST- and His6-Myc-fusion proteins ...
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bioRxiv - Cell Biology 2023Quote: ... Conventional reverse transcription with random hexanucleotide primers was then performed using the RNA to cDNA EcoDry Premix (639549, TaKaRa) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... was amplified with primers Fe-227S and Fe-204R using SYBR Premix Ex Taq II (Tli RNaseH Plus; Takara) (Table 2) ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... The synthesized cDNA was diluted (1:10) and used as template for quantitative real-time PCR (qRT-PCR) using SYBR premix EX-Taq-plus (TaKara) and analyzed on QuantStudio 6 Real-Time PCR machine (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... DNA was isolated from the expanded single colonies when confluent enough and used in PCRs using oligos to amplify exon 2 of CLN6 with CloneAmp HiFi PCR Premix (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Real-time PCR was carried out in triplicate with primers by using SYBR Green Realtime PCR Master Mix-Plus (Toyobo) in the Thermal Cycler Dice Real-Time PCR system (TaKaRa). PCR primers are listed in Supplemental Tables S2 and S3.
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bioRxiv - Immunology 2020Quote: ... The coding region of ADAR1 p150 was amplified by PCR and the resultant PCR product was inserted into a pEGFP-C1 vector (Clontech) using XhoI/BamHI restriction enzyme–recognition sites to generate the expression construct ...
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bioRxiv - Physiology 2021Quote: ... The RNA (1 ug/sample) was reverse transcribed into cDNA and Q-PCR was performed using a SYBR Green PCR kit (Takara) in a Light Cycler (Eppendorf) ...
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bioRxiv - Microbiology 2020Quote: ... The 1.5 kb PCR product was isolated and purified from agarose gel using the NucleoSpin Gel and PCR Clean-up kit (Takara). The product was subjected to Sanger dideoxy sequencing at GENEWIZ (San Diego ...
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bioRxiv - Cell Biology 2021Quote: ... Semi-quantitative PCRs (sqPCRs) were performed on 3 μl of cDNA template using Emerald Amp Max PCR Master Mix (Takara). The sequences of the primers used for PCR amplifications are included in Supplemental Table 4.
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bioRxiv - Cell Biology 2021Quote: ... cDNA was further amplified by adding 40 μl of a PCR mix containing 0.03 U/μl of Terra PCR direct polymerase (Takara Bio), 1.25x Terra PCR Direct Buffer ...
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bioRxiv - Cell Biology 2021Quote: Site-directed mutagenesis of pLenti-CMV-Neo-PINK1 (C125G)-EYFP or pLVX-puro-OMA1 (E328Q)-EYFP was performed by PCR amplification (CloneAmp HiFi PCR Premix, Takara or Q5 High-Fidelity DNA Polymerase system ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was isolated using the TRIzol method and the expression of miRNAs analyzed by qRT-PCR with the Mir-X miRNA qRT-PCR TB Green kit (Takara Bio Inc ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative real-time reverse transcription-PCR (qRT-PCR) was performed using the TB Green Premix Ex Taq II (TaKaRa, Japan) with the LightCycler® 96 system (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... The elute was diluted such that no more than 1.5ug of DNA was used per PCR reaction and amplified for 20 cycles of PCR using Primestar GXL (Takara Bio), run on a 1% agarose gel ...
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bioRxiv - Biophysics 2022Quote: ... eGFP-PolyQ31 was adapted from eGFP-PolyQ74 through the variability of Q-length PCR products amplified using CloneAmp HiFi PCR Premix (Takara). FM5-NPM1-mCh was generated as described in 27.
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bioRxiv - Molecular Biology 2020Quote: ... The sgRNA target locus on exon 3 or 5 was PCR amplified with Terra™ PCR Direct Polymerase Mix (Takara) for 35 cycles using primer sets mEX3F and mEX3R or mEX5F and mEX5R (Table 1) ...
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bioRxiv - Neuroscience 2022Quote: ... washed with 200 µL water, and resuspended in PCR mix (22 µL water, 25 µL Terra PCR direct buffer, 1 µL Terra Polymerase (Takara), 1 µL 100 M Truseq PCR handle primer ...
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bioRxiv - Zoology 2019Quote: ... were obtained from the mRho.V5.mER.hOr47a construct via PCR using the Advantage 2 PCR kit (Cat. Nr. 639206, Takara, Kusatsu, Japan) using the E.hOr47a_fwd and hOr47a_fwd forward primers ...
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bioRxiv - Molecular Biology 2021Quote: ... A 150-250 bp region encompassing each targeted locus was PCR amplified from ∼40 ng genomic DNA with ends containing partial Illumina adapter sequences using CloneAmp HiFi PCR (Takara). Reaction conditions were as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... Homology arms complementary to the pUC19 cloning vector were then added to the PCR product with another PCR in order to clone the RACE products with the In-Fusion HD Cloning kit (Takara). Individual clones were selected and Sanger sequenced.
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bioRxiv - Cell Biology 2022Quote: ... cDNA was amplified by PCR by adding 1 µl of the Terra PCR Direct Polymerase (1.25 U/µl, Takara 639270), 25 µl of the Terra PCR Direct buffer and 1 µl of the ISPCR primer (10 µM stock concentration ...
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bioRxiv - Plant Biology 2022Quote: ... were chemically synthesised and cloned by PCR into the pJET1.2 based gRNA vectors using Clontech CloneAmp HiFi PCR Premix (Takara, Japan). The transformation vector pICU2:Cas9-dsRED containing the Cas9 gene from Streptococcus pyogenes expressed under the promoter of the Arabidopsis Incurvata 2 gene (ICU2 ...
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bioRxiv - Molecular Biology 2022Quote: Two hundred nanogram of DNAs were used as a template for polymerase chain reaction (PCR) by using EmeraldAmp MAX PCR mater mix (Takara). First 30 cycles of PCR were performed using forward and reverse primers below ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on three biological replicates using the Thermal Cycler Dice Real Time System TP-760 (Takara) with the Kapa SYBR fast universal qPCR kit (Kapa Biosystems) ...
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bioRxiv - Genomics 2023Quote: ... A total of 3.5 μl ribo-depleted material was used for SMARTer (SMARTer PCR cDNA Synthesis Kit, catalog num. 634926 and Advantage 2 PCR kit, catalog num. 639207, Clontech-Takara) protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The construct containing Y181G-coding point mutation was generated by inverse PCR of Zdhhc20WT plasmid using CloneAmp HiFi PCR Premix (Takara) and joining the ends of the PCR product using InFusion cloning.
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR products of the exons were assembled into one full-length sequence using overlapping PCR (In-fusion cloning, Clontech) for each TAS1R and were then subcloned into the pEAK10 expression vector (Edge Biosystems ...
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Mutations in HUA2 restore flowering in the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutantbioRxiv - Plant Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using TB Green™ Premix Ex Taq™ II (Takara, Dalian, China). Relative gene expression was calculated using the 2−ΔΔCt method (Rao et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Final cDNA samples were used then used for quantitative real-time PCR assay by SYBR Green PCR Kit (RR820A, Takara). Quantitative primers were designed based on the gene sequences by Sangon Biotech ...
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bioRxiv - Immunology 2024Quote: ... we ran our linearized plasmid backbone and 83 bp PCR products on a 1% agarose gel and recovered each using the Gel and PCR Clean-up Kit (Takara) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The deletion of each Stx2a phage in the CmR transformants was confirmed by colony PCR using EmeraldAmp MAX PCR Master Mix (TaKaRa) and specific primers ...
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bioRxiv - Developmental Biology 2024Quote: ... Equimolar amounts of PCR products were mixed and a PCR was made with a primeSTAR GXL DNA polymerase (Takara Bio) without primers for the first 10 cycles using the following thermocycler conditions (95°C 3min ...
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bioRxiv - Developmental Biology 2021Quote: ... using SYBR Green PCR Master Mix (Takara, Japan). The relative expression levels of the target gene (EGFP ...
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bioRxiv - Neuroscience 2019Quote: ... PCR products were cloned into pEGFP-C1 (Clontech) using BamHI and SalI ...
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bioRxiv - Plant Biology 2020Quote: ... the In-Fusion Advantage PCR Cloning Kit (Clontech) and Gateway Cloning Technology (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR fragments were cloned into pLVX-puro (Clontech) lentivirus vector with Gibson Assembly Mixture (NEB ...