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Citations for Takara Bio :
1351 - 1400 of 3733 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The synthesized cDNA was subjected to RT-qPCR using TB Green Premix Ex Taq II (Takara Bio). The PCR conditions were set as follows ...
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bioRxiv - Immunology 2022Quote: ... 1 μg of total RNA was used to synthesize cDNA with PrimeScript RT reagent kit (RR047A, Takara) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Complementary DNA (cDNA) was prepared using the 5X Prime Script RT Master Mix (Takara RR-036A-1) with 1 μg of total RNA ...
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bioRxiv - Neuroscience 2022Quote: ... total RNA was reverse transcribed to cDNA using the PrimeScript™ RT reagent Kit (TaKaRa, RR037A, Japan). Convergent and divergent primers were used to detect the expression of linear and circular RNA transcripts ...
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bioRxiv - Neuroscience 2022Quote: ... These RNA samples were then reverse transcribed into cDNA by PrimeScript™ RT Reagent (TaKaRa, RR036A, Japan). Real-time polymerase chain reaction (RT-PCR ...
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bioRxiv - Plant Biology 2021Quote: ... gDNA removal and first-strand cDNA synthesis were conducted by PrimeScriptTM RT reagent Kit (Takara, Beijing, China) with 0.5 μg total RNA for each sample ...
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bioRxiv - Plant Biology 2021Quote: ... The first strand of cDNA was synthesized using PrimeScript™RT Master Mix (Perfect Real Time) (TaKaRa). Quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Neuroscience 2020Quote: ... spectrophotometer followed by reverse transcription reaction to produce cDNA using PrimeScript RT Reagent Kit (Takara, Clonetech, Japan). Specific primers against the genes of interest (Table 3 ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed with a PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Cat no. RR047A) and qRT-PCR was performed on StepOne Plus Real-time PCR system (Applied Biosystem ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μL of the RT reaction was combined with 2.5 μL of 10X Advantage 2 buffer (Takara), 2.5 μL of 2.5 mM dNTPs (Takara) ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized from equivalent total RNA using PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and cDNA was synthesized with the PrimeScript RT reagent Kit with the gDNA Eraser (TaKaRa Bio Inc.). Real-time PCR was performed using the KOD SYBR qPCR Mix (TOYOBO ...
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bioRxiv - Cell Biology 2019Quote: ... Extracted total RNA were quantified and reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (TaKaRa). All qPCR reactions were performed as 10 μl reactions using TB Green™ Premix Ex Taq™ II (TaKaRa) ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 500 ng total RNA using PrimeScript RT reagent Kit (Takara Bio., Shiga, JAPAN). The absence of genomic DNA contamination was confirmed by PCR using a control without reverse transcriptase ...
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bioRxiv - Genetics 2020Quote: ... according to the manufacturer’s instructions and cDNAs were prepared using PrimeScriptTM RT Master Mix kit (Takara, RR036A). Analysis of mRNA expression was performed with SYBR Green Master Mix (Life Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... then RNA was reverse-transcribed into cDNA using PrimeScript RT reagent with gDNA Eraser (Takara, Kusatsu, Japan). TB Green (Takara ...
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bioRxiv - Cell Biology 2021Quote: ... One-step qPCR was performed using One Step PrimeScript™ III RT-qPCR Mix (Takara, Kusatsu, Japan) with primers as follows ...
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bioRxiv - Physiology 2021Quote: 200 ng of mRNA were reverse-transcribed to cDNA using the PrimeScriptTM RT reagent kit (RR037A; Takara). Fluorescence-based quantitative real-time PCR (qRT-PCR ...
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Transcriptome Analysis of xa5-mediated Resistance to Bacterial Leaf Streak in Rice (Oryza sativa L.)bioRxiv - Plant Biology 2019Quote: ... First-strand cDNA synthesis was performed using PrimeScript™ RT reagent kit with gDNA eraser (Takara, Japan) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... first-strand cDNA was synthesized by using a PrimeScript RT reagent kit (Perfect Real Time) (TaKaRa Bio) with random hexamer primers and extracted RNA ...
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bioRxiv - Genomics 2021Quote: ... RNAs were reverse transcribed by using the PrimeScript RT reagent Kit with gDNA Eraser (TAKARA, Kyoto, Japan). Quantification of candidate genes was conducted in triplicate by using Bio-rad iQ SYBR Green Supermix (Bio-rad ...
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bioRxiv - Genetics 2021Quote: ... First strand cDNA was synthesized using PrimeScript™ RT reagent kit with gDNA Eraser (Takara, Dalian, China). After cDNA synthesis ...
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bioRxiv - Cancer Biology 2021Quote: ... Synthesis of Complementary DNA was performed by PrimeScript™ RT reagent kit (Takara Bio Inc., Shiga, Japan), and flowingly used as template for Real-time PCR reaction which was conducted by IQ5 (Biorad ...
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bioRxiv - Microbiology 2021Quote: ... Samples were incubated 30 min at RT and loaded onto a 10 μL aliquot of Talon (Takara) resin ...
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bioRxiv - Immunology 2020Quote: ... followed by cDNA synthesis using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara-Bio, Kusatsu, JAPAN). RT-qPCR using Brilliant II SYBR® Green QPCR Master Mix (Agilent ...
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bioRxiv - Neuroscience 2022Quote: ... Reverse transcription with 1 μg of total RNA was conducted using PrimeScript™ RT Master Mix (Takara) following the manual ...
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bioRxiv - Cell Biology 2022Quote: ... and mRNA reverse transcription was performed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan). The qPCR was performed using SYBR Green qPCR Master Mix (Takara ...
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bioRxiv - Genomics 2022Quote: ... and first-strand cDNA was synthesized using a PrimeScript RT Reagent Kit (Catalog No. RR037A, TaKaRa, Tokyo), which was used for subsequent transcriptomic analyses ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green supermix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green supermix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... then RNA was reverse-transcribed into cDNA using PrimeScript RT reagent with gDNA Eraser (Takara, Kusatsu, Japan). TB Green (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green super mix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green super mix (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... the corresponding cDNA was transcribed using a Fast Quant RT Kit with gDNA (TaKaRa Bio, Shiga, Japan). The cDNA was used as a template for RT-PCR assays ...
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bioRxiv - Molecular Biology 2022Quote: ... the total RNA was reverse transcribed to cDNA using the Prime ScriptTM RT reagent kit (TaKaRa Biotechnology). qPCR was performed using the SYBR Premix Ex Taq II Kit (TaKaRa Biotechnology ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized with 1 μg of total RNA using the Prime ScriptTM RT Reagent Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Then the cDNA was synthesized using PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa). Real-time quantitative PCR was performed as previously described [13 ...
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bioRxiv - Immunology 2023Quote: ... 500 ng of RNA was used for cDNA synthesis using a PrimeScript RT Reagent Kit (Takara, RR037A). 2 μL of diluted cDNA (1:50 ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from 200 ng of total RNA by Prime script RT master Mix (Takara, RR036A). RT-qPCR was performed using TB Green Premix Ex TaqII (Tli RnaseH Plus ...
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bioRxiv - Biochemistry 2022Quote: ... from which cDNA was obtained by reverse transcription using the PrimeScript RT Reagent Kit (TaKaRa, Kusatsu, Japan). Transcript levels of RvNaRL were examined using a real-time PCR system (LightCycler 96 system ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (400 ng) was then reverse-transcribed using PrimeScript RT Master Mix (#RR036A; TaKaRa, Kusatsu, Japan). The cDNA was then subjected to the subsequent PCR using TB Green Premix Ex™ Taq II (Tli RNaseH Plus ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed using a TB Green®Premix Ex Taq™II (TaKaRa, Lot: AL51019A), and β-actin gene was used as a reference gene ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA (1 μg) was reverse transcribed with the PrimeScript™RT Reagent Kit (Takara Bio Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized from 500 ng RNA using a Primescript RT Reagent Kit (Takara Bio, Shiga, Japan) with random hexamer and oligo(dT ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cDNA was reverse transcribed by using a PrimeScript RT Reagent Kit Perfect Real Time (TaKaRa Bio Inc.). Real-time PCR was performed using a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and cDNA was synthesized from the extracted RNA using PrimeScriptTM RT Reagent Kit (Takara Bio Inc., Japan) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of total RNA was then reverse transcribed in 10 µL reactions using PrimeScript RT (TAKARA) and random hexamer primers ...
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA was prepared using the Prime Script RT Reagent Kit (Takara Bio, Shiga, Japan; RR047B) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... cDNA reactions were performed according to the manufacturer’s instructions of the PrimeScript™ RT Reagent Kit (Takara) using primers specifically targeting the U6 and scaffold region (forward primer 5’-CCGACTCGGTGCCACTTTTT-3’ ...
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bioRxiv - Cancer Biology 2023Quote: mRNA was isolated and cDNAs were synthesized by using a Prime Script RT Reagent Kit (TaKaRa, China) according to manufacturer’s instruction ...