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Citations for Takara Bio :
1451 - 1500 of 3733 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... PCR was performed using TaKaRa ExTaq (TaKaRa). PCR products were analyzed using a 1% agarose gel ...
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bioRxiv - Molecular Biology 2023Quote: ... SapphireAmp Fast PCR Master Mix (Takara Bio) was used for verifying E.coli and M ...
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bioRxiv - Developmental Biology 2023Quote: ... Nested PCR was performed with rTaq (Takara), followed by the PCR product fragments sequencing with Sanger sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted gDNA was PCR amplified (Takara, RR001A) using a 30-cycle protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted gDNA was PCR amplified (Takara, RR001A) using a 30-cycle protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR was performed with ExTaq (TaKaRa, Japan) on 2720 thermal cycler (Applied Biosystems) ...
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Docking Domain Engineering in a Modular Polyketide Synthase and its Impact on Structure and FunctionbioRxiv - Biochemistry 2023Quote: CloneAmp HiFi PCR Premix was from Takara. Primers were synthesized by Sigma Aldrich ...
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bioRxiv - Genetics 2023Quote: ... Bisulfite PCR was performed using EpiTaq (Takara) using custom made locus specific primers containing 4 bp unique molecular identifiers (UMI ...
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bioRxiv - Cancer Biology 2023Quote: ... using SYBR Green PCR Master Mix (Takara), and transcript levels were normalized to actin as an internal control ...
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bioRxiv - Molecular Biology 2023Quote: ... and SapphireAmp Fast PCR Master Mix (Takara). The PCR conditions were as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR was performed using PrimeSTAR MAX (TAKARA) with primers (Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR fragments containing pLib vector backbone (Clontech) and EF1A promoter ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in a 20 μL reaction containing SYBR Green (TaKaRa, Kusatsu, Japan) on MXP3000 cycler (Stratagene ...
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bioRxiv - Genomics 2021Quote: ... tricornutum were amplified by PCR was amplified by PCR using PrimeSTAR Max DNA polymerase (TaKaRa Bio Inc., Shiga, Japan) and the primer (pFs_nptII_1_F ...
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bioRxiv - Microbiology 2020Quote: ... The number of viral copies was detected by real-time quantitative reverse transcription PCR (qRT-PCR) (Takara Cat no.RR820A) to reflect the replication efficiency of the virus ...
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bioRxiv - Systems Biology 2022Quote: ... The DNA regions of interest were amplified from host plasmids by PCR using CloneAmp HiFi PCR Premix (Takara, 639298) with custom primers for Gibson assembly (with overhangs) ...
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bioRxiv - Biochemistry 2022Quote: ... N-terminal Flag-tagged P38α containing 3C protease site was amplified by PCR using CloneAmp HiFi PCR Premix (Takara) and ligated in to pcDNA3 using the aforementioned restrictions sites ...
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bioRxiv - Cell Biology 2020Quote: ... was used to PCR amplify the PLIN2 cDNA and gel purified via NucleoSpin Gel and PCR Clean Up (Takara, Mountain View ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The product from this PCR was purified and cleaned with NucleoSpin Gel and PCR clean-up kit from TAKARA. A 2nd PCR was performed with high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and then used as the template to synthesize double strand cDNA amplicons by PCR (optimal 17 – 21 cycles) using Advantage 2 PCR kit according to the manufacturer’s specifications (Clontech). cDNA was purified using NucleoSpin Gel and PCR Clean-up kit (Takara Bio) ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcode sequence of each clone was PCR amplified directly from the cells using Terra PCR Direct Polymerase kit (Takara) for Sanger sequencing using previously described primers (5).
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bioRxiv - Microbiology 2024Quote: ... The guides were introduced into the pEcgRNA vector through reverse PCR using the CloneAmp HiFi PCR Premix (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed using Takara PrimeScript™ RT Master Mix (Clontech Laboratories, USA). qRT-PCR was performed on Step One Plus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... 500 ng total RNA was reverse-transcribed to cDNA (Prime-Script RT reagent kit, Takara Bio, Dalian, China). The parameters set for reverse transcription were as follows ...
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bioRxiv - Developmental Biology 2020Quote: ... Total RNA was converted to cDNA using PrimeScript RT reagent Kit with gDNA Eraser (RR047A, Takara, Dalian, China). The mRNA expression levels of the Ccl9 ...
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bioRxiv - Microbiology 2022Quote: ... The first-strand cDNA was synthesized using reverse transcriptase from the PrimeScript RT reagent kit (Takara Biotechnology, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and quantitative reverse transcription-polymerase china reaction (RT-qPCR) was performed using SYBR Premix Ex Taq (TaKaRa, China). All procedures were performed following the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2019Quote: ... the cDNA was synthesized by reverse transcription using PrimeScript RT reagent kits with gDNA Eraser (Takara, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... cDNA was generated from 1 μg of RNA using the PrimeScript RT reagent kit with gDNA Eraser (Takara). The mRNA quantifications of target genes were performed by real-time PCR using the SYBR GREEN MIX (Takara) ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng RNA was DNAse I-treated (ThermoFischer Scientific) and submitted to reverse transcription (Takara PrimeScript™ RT). Before qPCR ...
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bioRxiv - Pathology 2019Quote: ... and treated with DNase I following the manufacturer’s instructions (PrimeScript RT reagent Kit with gDNA Erase, Takara, Japan). cDNA synthesized from reverse transcription of RNA samples was used to determine the mRNA expression levels of target genes as well as for quantifying TYLCV accumulation levels at the date indicated ...
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bioRxiv - Microbiology 2019Quote: ... the total RNA (1 µg) was reverse transcribed by PrimeScript RT reagent Kit with gDNA Eraser (Takara, China). The IL-1β and components of RISC mRNA expression levels of the NA cells were quantified with the SYBR Green qPCR kit (Takara ...
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bioRxiv - Cell Biology 2019Quote: ... approximately 6 μg RNA was applied for 1st-strand cDNA synthesis using PrimeScriptTM RT Reagent Kit (TaKaRa, AK6003) with oligo (dT ...
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bioRxiv - Genomics 2020Quote: ... cDNA was generated via PrimerScriptTM RT Reagent Kit with genomic DNA Eraser (Perfect Real Time) (TaKaRa, Beijing, China). Quantitative real-time PCR analysis (q-PCR ...
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bioRxiv - Cell Biology 2021Quote: The mRNA and miRNA cDNA libraries are reverse-transcribed by PrimeScript™ RT Master Mix (TaKaRa, Dalian, China) and miRNA 1st Strand cDNA Synthesis Kit (by stem-loop ...
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bioRxiv - Plant Biology 2021Quote: ... and was reverse transcribed by the PrimeScriptTM RT reagent Kit with gDNA Eraser following the manufacturer’s instructions (TaKaRa). RT-qPCR was performed with SYBR Green mix (Bimake) ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was done using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, #RR047A). qPCR reaction was prepared by TB Green® Premix Ex Taq™ (Tli RNaseH Plus ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA was synthesized by reverse transcription of 1 μg RNA using PrimeScrip RT Master Mix(Takara). Quantitative real time-PCR was performed using TB Green Premix Ex Taq (Takara ...
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bioRxiv - Cell Biology 2021Quote: ... 2 µg of total RNA was subsequently reverse transcribed to cDNA using PrimeScript RT Master Mix (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ng of total RNA was reverse-transcribed using the PrimeScript RT Reagent Kit (Takara Bio, Shiga, Japan) and a T100 thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2022Quote: ... and reverse transcription was performed to generate cDNA using the PrimeScript RT Master Mix (RR036; Takara, Kusatsu, Japan). The RNase-Free DNase Set (79254 ...
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized using 2 μg RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa). Gene specific primers for quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Pathology 2022Quote: ... Single-stranded cDNA was reverse transcribed using Prime Script RT Master Mix (Perfect Real Time) (Takara Bio Inc.), and used for subsequent real-time PCR reactions ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR for mRNA detection was performed with the SYBR Premix Ex Taq II (Takara Biomedical Technology, China) using Bio-Rad iQ5 detection instrument (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized from 500 ng total RNA using the PrimeScript RT reagent kit (Takara, Japan). Quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Plant Biology 2021Quote: ... Isolated RNA was used as template for cDNA synthesis using PrimeScript RT Reagent Kit (Takara Bio, Kusatsu, Japan). Transcript expression was quantified by qRT-PCR using iQ SYBR Green supermix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed with 1 μg of total RNA using the PrimeScript™ RT Reagent Kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The reverse transcription of RNA into cDNA was performed using a Prime Script TM RT Master Mix (Takara). The following was the reaction system ...
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bioRxiv - Developmental Biology 2022Quote: ... 500 ng of total RNA was reverse-transcribed into cDNAs using the PrimeScript RT Reagent Kit (TaKaRa, RR037A) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: cDNA was generated from 1 μg of total RNA by using the PrimeScript RT Reagent Kit (Takara, Japan) in accordance with the manufacturer’s protocol as previously described [21] ...