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Citations for Takara Bio :
1301 - 1350 of 3733 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: cDNA was synthesized from 300–500 ng total RNA using the PrimeScript RT Master Mix (Takara) in a volume of 10 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... and reverse transcribed into cDNA using a PrimeScript RT Master Mix (Takara RR036A, Kusatsu, Shiga, Japan). Quantitative real-time RT-PCR analysis was performed using TB Green Premix Ex Taq (Clontech ...
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bioRxiv - Immunology 2023Quote: ... and was retrotranscribed to cDNA with PrimeScript™ RT reagent Kit with gDNA Eraser (Takara, RR047A). Real-time quantitative RT-PCR (qPCR ...
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bioRxiv - Microbiology 2023Quote: ... One microgram of RNA was transcribed into cDNA using the PrimerScript RT Reagent Kit (Takara, Japan). After reverse transcription ...
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bioRxiv - Immunology 2023Quote: ... cDNA synthesis from total RNA using the Prime Script RT Reagent Kit (Cat. No. RR047A, Takara) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... was reverse transcribed using PrimeScript™ RT Reagent Kit (Perfect Real Time) (Takara Bio, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The isolated RNA was reverse transcribed using the PrimeScript™ RT reagent kit (Takara Bio Inc.). Reverse-transcribed cDNA was amplified using PrimeStar DNA polymerase (Takara Bio Inc. ...
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bioRxiv - Plant Biology 2023Quote: ... First-strand cDNA was synthesized with a PrimeScript™ RT reagent kit with gDNA Eraser (Takara). For gene expression pattern analysis ...
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bioRxiv - Plant Biology 2023Quote: ... 500 ng total RNA was reverse transcribed into cDNA with the PrimeScript RT Master Mix (TaKaRa). qPCR was performed using corresponding primers listed in SI Appendix ...
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bioRxiv - Plant Biology 2024Quote: ... The first-strand cDNA synthesis was performed with the Prime Script RT Master Mix kit (Takara). Quantitative real-time PCR was performed at 60°C using Takyon No ROX SYBR 2X MasterMix blue dTTP (Eurogentec ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription of RNA to cDNA was performed using the PrimeScript RT Reagent kit (RR037A, Takara). Quantitative RT-PCR was then performed on the cDNA using TB Green Premix Ex Taq II (RR820A ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was carried out using TB Green® Premix Ex Taq™ GC (TaKaRa, RR071Q). Gene expression data were normalized to sigA and expressed as fold change using the 2−ΔΔCt method compared to wild-type strain or untreated control.
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bioRxiv - Developmental Biology 2023Quote: ... and reverse transcribed to synthesize cDNA using a PrimeScript RT Master Mix reverse transcription kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The cDNA was obtained by using the PrimeScript RT Reagent Perfect Real Time kit (Takara, Japan). Briefly ...
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bioRxiv - Pathology 2024Quote: ... following the manufacturer’s instructions and used for reverse transcription with the PrimeScriptTM RT reagent Kit (TaKaRa).The sequences of FaMyo5 motor domains were amplified from the cDNAs of all the F ...
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bioRxiv - Cell Biology 2023Quote: ... 400 ng of RNA was used for reverse transcription using PrimeScript RT Master Mix (Takara, USA). PCR reactions were performed using TB Green Premix Ex Taq (Takara ...
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bioRxiv - Immunology 2023Quote: ... and then converted to cDNA using PrimeScript™ RT Master Mix (Perfect Real Time, Takara, RR036A). Quantitative real-time reverse transcription-polymerase chain reaction (qPCR ...
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bioRxiv - Plant Biology 2019Quote: cDNA was synthesized with oligo(dT) primers using PrimeScript™ 1st strand cDNA synthesis kit (Takara, Japan). Quantitative RT-PCR analysis was performed on an Agilent Real-Time qPCR apparatus (Mx3000P system ...
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bioRxiv - Biochemistry 2020Quote: ... reverse P4 or P5 primers and PrimeStar GXL DNA polymerase (Takara Bio Inc., Mountain View, CA, USA). The resulted PCR product was cloned into TetR-DOZI plasmid49 by sequence- and ligation-independent cloning (SLIC ...
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bioRxiv - Genetics 2020Quote: MtDNA was amplified from 50 ng of total DNA with the primers (LongR_mtDNA_Fw and LongR_mtDNA_Rv, Table 1) using PrimeSTAR GXL DNA polymerase (TAKARA, Japan) and following PCR conditions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... pre-amplified (DFRS empty plasmid primers) and pre-digested by the same enzymes (In Fusion Kit; Clontech). The miR-137 or miR-122 complementary sequences were thereby located on the 3’UTR of the RFP gene.
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bioRxiv - Molecular Biology 2022Quote: ... using the primers described previously(6) and cloned into pmCherry-C1 (Takara Bio Inc., San Jose, CA). To silence HNRNPK expression ...
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bioRxiv - Immunology 2022Quote: ... Rearranged VDJ IGM and IGG amplicons were generated using a universal forward primer (Takara Bio;10 μM), and either an IgM-CH3 (5’-CAGATCCCTGTGAGTCACAGTACAC-3’ ...
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bioRxiv - Molecular Biology 2020Quote: ... Aliquots of total RNA (1 μg) were reverse transcribed using pd(N)6 random primer (Takara Bio) and Moloney murine leukemia virus (M-MLV ...
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Retrovirus-derived RTL9 plays an important role in innate antifungal immunity in the eutherian brainbioRxiv - Evolutionary Biology 2023Quote: ... 200 μM each of NTP and 800 nM of primers along with 0.625 units ExTaq HS (TaKaRa) were subjected to 32 cycles at 96°C for 15 s ...
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bioRxiv - Neuroscience 2024Quote: ... 1μg of total RNA was reverse transcribed using an oligo-dT primer and PrimeScript RTase (TaKaRa (Japan), 2680A) ...
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bioRxiv - Cell Biology 2024Quote: ... Actin and GAPDH (primers in Table 1) in ovaries by using TB Green syber mix (Takara - RR820A) as per manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR was performed on a TP-850 Real-Time PCR Detection System (TAKARA Bio) using SYBR Green Premix ExTaq II ...
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bioRxiv - Plant Biology 2020Quote: ... PCR was performed using the SYBR-Green PCR Master mix (SYBR® Premix Ex Taq™, Takara) and a CFX96 thermal cycler (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... and quantitative real-time PCR (q-PCR) was carried out using SYBR Premix Ex Taq (TaKaRa, Japan). Q-PCR was carried out in a Bio-Rad CFX96 system ...
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bioRxiv - Molecular Biology 2020Quote: ... Reverse PCRs were performed in two rounds using CloneAmp HiFi PCR premix (Clontech, Mountain View, CA, USA). The first round was performed adding the forward and reverse primers in two independent reactions and consisted of 3 cycles of 98 °C for 10 seconds ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-Up kit (Takara Bio, Kusatsu, Shiga, Japan), and 600 ng-1 ug of DNA templates were added to set up in vitro transcription reactions ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative PCR was performed using the Mir-X miRNA qRT-PCR TB Green Kit (Takara Bio, USA) using the protocol provided by the manufacturer ...
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bioRxiv - Genomics 2023Quote: ... The obtained PCR products were purified using NucleoSpin Gel and PCR Cleanup kit (Takara Bio, Shiga, Japan) and sequenced directly or were cloned into the pTAC-1 vector (Biodynamics ...
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bioRxiv - Cancer Biology 2023Quote: ... 1-st PCR (PCR 1) was performed using Titanium Taq DNA Polymerase (# 639209, Takara Bio, CA, USA). Separation of the PCR products from primers and gel purification was done by QIAquick PCR & Gel Cleanup Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed by SYBR Green PCR Master Mix (Takara). The primer sequences for qRT-PCR were listed in Table S15.
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bioRxiv - Developmental Biology 2024Quote: ... 1 ul of the cDNA was used for PCR reaction using CloneAmp HiFi PCR Premix (Takara, 639298) with the following primers ...
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bioRxiv - Genetics 2023Quote: ... The PCR produc t was purified with a PCR purification kit (Takara Biomedical Technology, Bei jing, China) and was cloned into a pMDTM18-T vector (Takara Biomedical Tec hnology ...
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bioRxiv - Biophysics 2021Quote: ... The first-strand cDNA was synthesized using a PrimeScript® RT reagent Kit (Takara, Otsu, Shiga, Japan). The PlMYB308 gene was PCR-amplified using specific primers (Table S6) ...
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bioRxiv - Genetics 2021Quote: ... RT-qPCR reactions were performed in triplicate with TaqMan Master Mix: Premix Ex Taq (TaKaRa, Otsu, Japan) in a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription was performed with a PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Cat no. RR047A) and qRT-PCR was performed on a StepOnePlus Real-time PCR system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA synthesized from 1 μg total RNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara). Real-time PCR was performed using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Cancer Biology 2019Quote: ... then 500 ng of total RNA was reversely transcribed into cDNA using PrimeScriptTM RT Master Mix (TaKaRa) according to the manufacturer’s instructions in a total volume of 10 μL ...
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Mutant ACTB mRNA 3′UTR Promotes Hepatocellular Carcinoma Development by Regulating miR-1 and miR-29abioRxiv - Cancer Biology 2019Quote: ... the isolated RNA was reverse transcribed to cDNA using a PrimeScript RT reagent Kit (Takara, Dalian China). Real-time PCR analyses were performed with SYBR Green (Takara ...
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bioRxiv - Developmental Biology 2019Quote: ... and then cDNA was synthesized using the PrimeScript reverse transcription (RT) reagent kit (6210B; Takara Bio. Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... total RNA was used for reverse transcription (RT) using the SMARTScribe Reverse Transcription Kit (TaKaRa, Cat #639537).
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bioRxiv - Neuroscience 2020Quote: ... We then added 0.6 μL RT-mix [16.7 U μL−1 SMARTScribe Reverse Transcriptase (Takara Bio, 639538), 1.67 U μl−1 Recombinant RNase Inhibitor (Takara Bio ...
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bioRxiv - Cancer Biology 2019Quote: ... and subsequently subjected to reverse transcription using PrimeScript™ RT reagent Kit (TaKara Bio, Mountain View, CA). Quantitative real-time PCR was performed with Premix Ex Taq (TaKara Bio ...
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bioRxiv - Microbiology 2020Quote: ... cDNAs from 1 μg DNase-treated purified RNA were obtained using PrimeScriptTM RT Master Mix (#RR036A, TAKARA) following the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2021Quote: ... synthesized cDNA with the ReverTra Ace qPCR RT Master Mix (TOYOBO) and used TaKaRa Ex Taq (TaKaRa) for amplification of NSP and MA genes ...