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Citations for Takara Bio :
1151 - 1200 of 2435 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The lentivirus-containing supernatant was concentrated around 40-fold using Lenti-X concentrator (Takara), aliquoted and stored in liquid nitrogen.
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bioRxiv - Cell Biology 2023Quote: ... filtered with a 0.45 μm syringe filter and concentrated using Lenti-X Concentrator (Clontech) and stored at -80°C for further use ...
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bioRxiv - Cancer Biology 2023Quote: HEK 293T cell line (Lenti-X™ 293T, Takara Bio, San Jose, CA, USA) was cultured in DMEM High Glucose (DMEM-HG cat# SH30243.02 ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing supernatants were first concentrated 10x using Lenti-X Concentrator (Takara, Cat# 631232). Single cell-suspended organoids were mixed with concentrated virus ...
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bioRxiv - Genetics 2023Quote: Lentiviruses were produced using the Lenti-X Tet-ON Advanced Inducible Expression System (Clontech), following the protocol described therein ...
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bioRxiv - Cell Biology 2023Quote: ... concentrated down 10 or 30 times using the Lenti-X Concentrator (631232; Takara Bio). Supernatants were kept at -80°C prior to being directly applied to target cells which were then spun at 700 xg for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... and the excised DNA was purified and transfected into Adeno-X 293 cells (TaKaRa). When late CPE was observed ...
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bioRxiv - Neuroscience 2023Quote: ... and then the virus solution was concentrated using the Lenti-X Concentrator (631231, Clontech) and suspended in PBS (in vivo conditions).
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bioRxiv - Systems Biology 2023Quote: ... Lentiviruses carrying sgRNAs were produced in Lenti-X 293T cells (Takara Bio, Cat#632180) by transfection and concentrated using Lenti-X concentrator (Takara Bio ...
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bioRxiv - Genetics 2023Quote: ... The viral solution was further concentrated using the Lenti-X™Concentrator (Takara: 631231) according to the manufacturer’s instructions and stored at -80°C for long-term use ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus was titered using the Lenti-X p24 Rapid Titer Kit (Takara Cat #632200).
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bioRxiv - Cancer Biology 2023Quote: Lentiviruses were generated by transient co-transfection of Lenti-X 293T cells (Takara, #632180). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Harvested viral supernatants were pooled and concentrated with Lenti-X Concentrator (Takara Bio, 631232) by centrifugation at 1,500 x g for 45 minutes ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... and lentivirus-containing supernatants were concentrated using Lenti-X concentrator (Takara Bio, Cat#631231). NPCs were transduced and selected using 4ug/ml puromycin (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and then lentivirus was harvested and concentrated using Lenti-X Concentrator (Takara Bio, 631232).
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bioRxiv - Neuroscience 2023Quote: ... was packaged as follows: 80% confluent Lenti-X™ 293T cells (Takara Bio; #632180) were transfected in 15-cm plates using Lipofectamine 3000 transfection reagent (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... The supernatants were collected and mixed with Lenti-X Concentrator (Takara Bio USA, Inc) at a 1:3 ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... viral particles were collected and concentrated using a Lenti-X concentrator (631231; Takara Bio). Lentiviral titers were measured using Lenti-X GoStix (PT5185-2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviruses were generated by seeding Lenti-X 293T cells (Takara Bio, Cat. No. 632180) in 6-well plates ...
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bioRxiv - Biochemistry 2021Quote: ... an aliquot of the lysate was used to measure protein concentration by BCA protein assay (Takara Bio). The lysate was mixed with Optiphase Hisafe 3 (PerkinElmer) ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations of cell lysates were measured using a bicinchoninic acid protein assay kit (Takara Bio Inc.). Cell lysate containing the same amount of protein was mixed with 10 volumes of methanol and then centrifuged at 20,000 × g for 30 min to precipitate proteins ...
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bioRxiv - Cell Biology 2020Quote: ... from second to third - 5’-ttgaattcgcgctttgtgagcattgc-3’ and 5’-ttgtcgacgttgtcgtccgtgtgcac-3’ and then subcloned into pGAD424 vector (Clontech) in frame with activation domain of GAL4 using restriction sites EcoR1 and Sall.
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bioRxiv - Microbiology 2023Quote: ... 5’RACE was performed with SMARTer RACE 5’/3’ Kit (Takara Bio USA, Inc. San Jose, CA USA) according to the manufacturer’s directions.
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bioRxiv - Biochemistry 2023Quote: ... ∼5 μg of bacmid were transfected using 5 μL of TransIT®-Insect transfection reagent (Takara Bio Inc.). 5 days after initial transfection ...
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bioRxiv - Cancer Biology 2019Quote: Full-length EML4 cDNA was isolated by PCR from human cDNA (Clontech) and subcloned into a version of pcDNA3 or pcDNA3.1-hygro (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... Human universal reference total RNA (Catalog No. 636538, Clontech, Mountain View, CA) was used as a template to synthesize cDNA by reverse transcription ...
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bioRxiv - Cell Biology 2021Quote: For expression analysis human multiple tissue cDNA panel (MTC™ Clontech, 636742) and human normal brain tissue qPCR array (OriGene Technologies ...
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bioRxiv - Cell Biology 2022Quote: Naïve human PSCs were cultured in the PXGL medium: Ndiff227 (Takara Bio) medium supplemented with PD0325901 (Sigma,1 μM) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... commercially available cDNAs originating from various human tissues were purchased from TaKaRa (detailed sample information is given in Table S3 ...
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bioRxiv - Genomics 2019Quote: ... Wild-type sequences were generated by PCR using human genomic DNA (Clontech) as a template ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... pooled total RNA of human kidney and liver were purchased from Clontech. Total RNA (2 µg ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 10 ng cDNA of various tissues (Takara Human MTC panel I & II) were used for each reaction and amplified by KOD Xtreme Hot Start DNA polymerase kit (Takara) ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA from human tissues was purchased from Clontech (catalog number 636643). Most of these samples represent pooled RNA from multiple individuals (between 2 and 63 individuals) ...
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bioRxiv - Neuroscience 2022Quote: ... were purchased from Addgene and Genscript or amplified from human adult and fetal brain RNA (Takara) (see Table S10)(Alford et al. ...
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bioRxiv - Cell Biology 2022Quote: ... ERManI-YFP was constructed with human ERManI cloned into peYFP-N1 (Clontech) using Hind III and Xma I yielding ERManI fused on its C-terminus to YFP through a 6 amino acid flexible linker (SGGGGS) ...
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bioRxiv - Cell Biology 2023Quote: Human CD34+ HSPCs were cultured overnight on RetroNectin-coated (Takara Bio USA) plates in StemSpan SFEM II medium supplemented with SCF 100 ng/mL ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from human liver and fetal brain was purchased from Clontech Laboratories ...
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bioRxiv - Developmental Biology 2024Quote: Human naïve cells were cultured in PXGL medium30: N2B27 medium (Takara, Y40002), supplemented with 1uM PD0325901 (Cambridge Bioscience ...
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bioRxiv - Genomics 2019Quote: ... 4μl 5× First-Strand buffer (Takara, #639538), and 1μl B-tag-sw oligo ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... 5’-ctcgagtgcggccgcaagcttgctcatgacgctgccacggtg-3’ using PrimeSTAR HS (Takara). The pET28a was digested at NdeI and HindIII sites ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... 5’-cagtggtggtggtggtggtgctcgagtcaacacctggcgttgaccg-3’ using PrimeSTAR HS (Takara). The pET28a-MBP was digested at BamH1 and XhoI sites ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μL dNTPs (25 mM; Takara Bio), 1 μL primers (10 pmol/μL each primer) ...
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bioRxiv - Immunology 2022Quote: 5 × 106 GP2-293 packaging cells (Clontech) were plated in a 10 cm dish containing D10 medium (DMEM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2020Quote: ... 4µl 5× First-Strand buffer (Takara, #639538), and 1µl B-tag-sw oligo ...
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bioRxiv - Immunology 2022Quote: ... the SMARTer RACE 5’/3’ Kit (Takara) was used following manufacturer’s protocol and using the following primer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the 5’-Full RACE Core Set (TaKaRa) was used to extend Rtl6 mRNA from the mouse brain at 8 weeks of age ...
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bioRxiv - Microbiology 2021Quote: ... a SMARTer RACE 5’/3’kit (Takara) was used ...
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bioRxiv - Plant Biology 2022Quote: ... 5 × PrimeScript™ RT Master Mix (TAKARA) was used to synthesize cDNA ...
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bioRxiv - Developmental Biology 2023Quote: SMARTer RACE 5’/3’ Kit from Takara Bio was used following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined via the Bradford method using the TaKaRa Bradford Protein Assay Kit (Takara Bio, T9310A).