Labshake search
Citations for Takara Bio :
801 - 850 of 6736 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... This RNA was used as input to the Mir-X™ miRNA qRT-PCR TB Green® Kit (Takara cat# 638316) to generate miRNA cDNA ...
-
bioRxiv - Evolutionary Biology 2023Quote: Complete cDNA sequences of taste receptors were obtained using a SMARTer 5’/3’ kit for RACE PCR to obtain 5’ sequence information missing from reference transcriptome (Takara, #634858). Briefly ...
-
bioRxiv - Molecular Biology 2024Quote: ... was PCR-amplified from SK-N-BE cells cDNA and cloned between the ICSs using In-fusion Cloning Kit (Takara Bio). 4xPepper array sequence (196 bp long ...
-
bioRxiv - Biochemistry 2024Quote: ... DNA fragments were joined through SOE-PCR and subsequently ligated into the pOPINK vector using the In-Fusion HD Cloning Kit (Takara Clonetech). A pOPINE vector containing C-terminally Flag-tagged human USP30 (1-517 ...
-
bioRxiv - Immunology 2024Quote: ... PCR experiments were performed in a total volume of 10 μl by using an SYBR Premix Ex Taq kit (Takara Bio). The reaction mixtures were incubated for 2 min at 95℃ ...
-
bioRxiv - Neuroscience 2024Quote: ... Titration of the concentrated viruses was performed by AAVpro Titration Kit (for Real Time PCR) Ver.2 (6233, Takara Bio Inc) following the manufacture’s protocol ...
-
6-Phosphogluconolactonase is critical for the efficient functioning of the pentose phosphate pathwaybioRxiv - Biochemistry 2024Quote: ... coli BW25113 WT strain by PCR and inserted in pZA23 plasmid using the In-Fusion® HD Cloning Plus CE Kit (Takara).
-
bioRxiv - Cell Biology 2024Quote: ... templates were constructed by cloning the 1.5 kb upstream and downstream homology arms into the pPD95.77 plasmid using the In-Fusion Advantage PCR cloning kit (Clontech, Cat. #639621). Fluorescence tags were inserted into the constructs with a flexible linker before the stop codons ...
-
bioRxiv - Neuroscience 2024Quote: ... The 3×FLAG-tagged hnRNPA1 cDNA was subsequently amplified from the p3xFLAG vector by PCR and cloned with the use of an In-Fusion Cloning Kit (Takara Bio) into the pEN_TTGmiRc2 vector (Addgene) ...
-
bioRxiv - Bioengineering 2024Quote: ... the amplified DNA fragments from the pooled 48 samples were purified by gel extraction using the NucleoSpin Gel and PCR Clean-up Midi kit (TaKaRa, 740986.20). We repeated this DNA purification process every 48 samples ...
-
bioRxiv - Developmental Biology 2024Quote: ... A partial length Mlβ-cat coding sequence was amplified from cDNA using the Advantage RT for PCR kit (Clontech, cat #639506) using the following primers:
-
bioRxiv - Plant Biology 2020Quote: CloneAmp HiFi PCR Premix (Takara) was used for all PCR amplifications ...
-
bioRxiv - Molecular Biology 2022Quote: ... by RT-PCR (TaKaRa, RR014) and cloned by Gibson assembly into the vector pK27SUMO (expressing a 14His-tagged version of the yeast SUMO protein Smt3 ...
-
bioRxiv - Microbiology 2021Quote: ... 1× ExTaq PCR buffer (Takara, Clontech Laboratories ...
-
bioRxiv - Microbiology 2021Quote: ... using CloneAmp HiFi PCR (Takara) and was subcloned in the Thymidine kinase Cypridina plasmid (Thermofisher ...
-
bioRxiv - Microbiology 2021Quote: ... Comparative RT-PCR (Takara, Japan) was performed using SYBR Green Supermix via an RT-PCR machine (RocheLightCycler480 ...
-
bioRxiv - Plant Biology 2023Quote: ... PCR was performed by TaKaRa EX Taq (Takara ...
-
bioRxiv - Biophysics 2023Quote: ... CloneAmp HiFi PCR Premix (Clontech) was used for all the PCR amplifications ...
-
bioRxiv - Biochemistry 2023Quote: ... by RT-PCR (Takara, RR014) and cloned by Gibson assembly into the vector pK27SUMO ...
-
bioRxiv - Molecular Biology 2024Quote: ... CloneAmp HiFi PCR Premix (Clontech) was used for all the PCR amplifications.
-
bioRxiv - Neuroscience 2020Quote: ... The construct was cloned into a lentiviral transfer vector containing the woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) (pLVX series, Clontech, Mountain View, CA) under the human synapsin 1 promoter (hSyn ...
-
bioRxiv - Biophysics 2022Quote: ... containing virus was collected after 48 hrs and concentrated by 50-fold following the protocol of Lenti-X™ Concentrator (Takara, Cat. #: 631232). The concentrated virus in 40uL volume was added to HEK293T cells plated several hours ahead started with 80,000 cells in one well of a 24-well plate ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 5 μg of total RNAs were reverse-transcribed into cDNA at 42°C for 1 h in 20 μl reaction mixture containing mouse Moloney leukemia virus reverse transcriptase (PrimeScript, TAKARA BIO, Shiga, Japan) with random 6 primers (TAKARA BIO) ...
-
bioRxiv - Genomics 2024Quote: Production of vesicular stomatitis virus (VSV-G) pseudotyped lentivirus was performed by calcium phosphate transfection of Lenti-X™ 293T cells (TaKaRa Clontech, 632180) using the CROP-mCherry-Fos construct and the helper plasmids pMD2.G and psPAX2 (Addgene plasmids 12259 and 12260) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Real-time PCR was performed using the SYBR Green PCR master mix (Takara Bio Inc.) according to the manufacturer’s specifications ...
-
bioRxiv - Neuroscience 2020Quote: ... Real-time PCR was carried out using SYBR Green PCR Master Mix (RR091A, Takara, Japan) and the quantitative analysis was performed using LightCycler Roche 480 qPCR instrument ...
-
bioRxiv - Microbiology 2020Quote: ... All PCR reactions were performed with CloneAmp HiFi PCR premix (Takara Bio, Mountain View, California) using 25 ng of DNA ...
-
bioRxiv - Pathology 2022Quote: Reverse transcription PCR (RT-PCR) was performed with 500 ng RNA per reaction by TaKaRa PrimeScriptTM RT Master Mix (Perfect Real Time ...
-
bioRxiv - Developmental Biology 2023Quote: Linear DNA fragments were amplified via PCR using CloneAmp HiFi PCR premix (Takara Bio, 639298). PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio ...
-
bioRxiv - Developmental Biology 2023Quote: ... Linear DNA fragments were amplified via PCR using CloneAmp HiFi PCR premix (Takara Bio, 639298). PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio ...
-
bioRxiv - Microbiology 2024Quote: ... CDS cDNA was then used as template for PCR with HiFi PCR mix (Takara Bio) to make each ORF compatible for In-Fusion cloning (Takara Bio) ...
-
bioRxiv - Microbiology 2024Quote: ... PCRs for cloning were performed with the proof-reading polymerase ClonAmp HiFi PCR Premix (Takara) and PCR for validation of strains were performed with EmeraldAmp GT Master Mix (Takara).
-
bioRxiv - Microbiology 2024Quote: ... PCRs for cloning were performed with the proof-reading polymerase ClonAmp HiFi PCR Premix (Takara) and PCR for validation of strains were performed with EmeraldAmp GT Master Mix (Takara).
-
bioRxiv - Physiology 2024Quote: ... Quantitative reverse transcriptase PCR (qRT-PCR) was conducted using SYBR Premix Ex Taq II (TAKARA) on a StepOnePlus real-time PCR system (Applied Biosystems ...
-
bioRxiv - Plant Biology 2020Quote: ... After 1 μg of total RNA from leaves was reverse-transcribed using a High Fidelity PrimeScript™ RT-PCR Kit (Takara, Japan), and Real-time qRT-PCR was performed using a Thermal Cycler Dice® Real Time System III T950 (Takara ...
-
bioRxiv - Plant Biology 2020Quote: One µg of total RNA was used to generate cDNA with the SMARTer PCR cDNA Synthesis kit (Clontech, Mountain View, CA, USA). Fifty µl of cDNA were amplified by 13 PCR cycles with the Kapa HiFi PCR kit (Kapa Biosystems ...
-
bioRxiv - Developmental Biology 2021Quote: ... 9 ul from each samples were directly used for cDNA amplification (15 cycles of LD PCR, using the SMART-Seq v4 Ultra Low Input RNA kit for sequencing (Takara Bio, #634898) and the SeqAmp DNA Polymerase (Takara Bio #638509) ...
-
bioRxiv - Developmental Biology 2020Quote: ... templates were constructed by cloning the 2 kb 5’ and 3’ homology arms into pPD95.77 plasmids using In-Fusion Advantage PCR cloning kit (Clontech, cat. no. 639621). We used the CRISPR design tool (http://crispr.mit.edu ...
-
bioRxiv - Developmental Biology 2020Quote: ... Pegl-17::GFP1-10 constructs were generated by cloning egl-17 promotor and GFP1-10 sequences into pPD95.77 plasmids using In-Fusion Advantage PCR cloning kit (Clontech, cat. no. 639621). The plasmid was injected into SPC-1::GFP11×7 knock-in animals ...
-
bioRxiv - Immunology 2022Quote: ... RT-PCR products were nested PCR-amplified with primers for IgG VH or IgG VL following the HS DNA polymerase kit protocol (Takara, TAK R010). The IgG VH and IgG VL nested PCR products were purified and next cloned into human IgG1 heavy chain and light chain expression vectors ...
-
bioRxiv - Microbiology 2020Quote: ... The qPCR reactions were done on Roche LightCycler® 96 PCR system with the SYBR Premix Ex Taq™ Kit (TAKARA, RR420A). Gene expression levels were normalized to GAPDH and compared to gene expression levels in hESCs ...
-
bioRxiv - Plant Biology 2021Quote: ... The PCR products were purified using Dyne PCR Purification Kit (DyneBio, South Korea) and cloned into pPZP211 vectors using Takara T4 DNA Ligase (Takara Bio, Japan). Escherichia coli (DH5α ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... served as template in a 20 μl qRT-PCR using the TB Green Premix Ex Taq II (Tli RNaseH Plus) kit (Takara Bio Inc.) and the Roche LightCycler 96 system (Roche ...
-
Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... The RNA was eluted according to the protocol in 10 uL nuclease free water and equal amounts of RNAs from different samples were immediately subjected to amplification-based cDNA synthesis using the SMARTer PCR cDNA Synthesis Kit (Takara, 634925, 639207). We have standardized the amplification cycle to 15 for 2-10 ng of initial RNA template used for the reaction ...
-
bioRxiv - Pathology 2022Quote: ... and expected amplicons of NS1 and VP1 genes were purified using Nucleospin® Gel and PCR clean-up kit (Takara Bio, Japan). Purified PCR amplicons were individually subjected to Barcode-Tagged sequencing (Celemics ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... library was prepared according to the Isoform Sequencing protocol (Iso-Seq) using the Clontech SMARTer® PCR cDNA Synthesis Kit (Clontech Laboratories (now Takara Laboratories), 634926 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Labelled amplicons were pooled and purified using NucleoSpin Gel and PCR Clean-up kits (Takara Bio USA, Inc, Mountain View, CA USA). Libraries were sequenced in-house using a NextSeq 500 High-Throughput Sequencing System (Illumina ...
-
bioRxiv - Neuroscience 2020Quote: ... RT-PCR analysis was performed using the TB Green® Premix Ex Taq™ Tli RNaseH Plus kit (TAKARA, Mountain View, CA). The gene-specific primer sequences were as follows ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The Rdl PCR-product was subcloned into the transcription vector pTB-207 38 using the In-Fusion® HD Cloning Kit (Clontech™) and cRNAs were synthesized with the T7 mMessage mMachine kit (Ambion™) ...
-
bioRxiv - Cancer Biology 2021Quote: ... according to instructions provided by the manufacturer and 2μg aliquots were reversed transcribed cDNAs using PrimeScript™ RT-PCR Kit (Takara, Dalian, China). After ...