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Citations for Takara Bio :
751 - 800 of 6736 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: The quantitative RT-PCR (qRT-PCR) was performed with SYBRGreen II (Takara, Dalian, China). The 7500 system (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-up (TaKara Bio Inc.), and then analyzed and quantified by Nanodrop spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2023Quote: ... The PCR products were purified using Nucleospin Gel and PCR Clean-up (Takara Bio). Nucleotide sequence analysis was outsourced from a commercial sequencing service (Eurofins ...
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bioRxiv - Biochemistry 2023Quote: ... qRT-PCR was carried out using SYBR Premi qRT-PCR ExTaq™ II (Takara) and analyzed on a Bio-Rad CFX96 real time PCR cycler (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Clonal lines were genotyped by PCR using EmeraldAmp GT PCR Master Mix (Takara, RR310A) and target specific primers flanking the Myrlin transcription start site ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each PCR reaction consisted of 25μL PrimeSTAR Max PCR mix (Takara Kusatsu, Shiga, Japan), 2 μM of each primer ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative PCR (qPCR) was performed using TB Green PCR Master Mix (TaKaRa, Dalian, China) on a Quantagene q225 qPCR System (Kubo ...
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bioRxiv - Cancer Biology 2021Quote: ... and quantitative real-time PCR was performed using the SYBR® Premix Ex Taq II Reagent Kit (Takara, Cat. Cat. #RR820A) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR amplified fragments and linearized vector by restriction enzyme digestion were assembled using In-Fusion HD Cloning Kit (Takara Bio Inc.). pEGFPN3-ARL4C was constructed as previously described(Matsumoto et al ...
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bioRxiv - Developmental Biology 2021Quote: ... three gene-specific reverse primers were designed and a nested PCR program was used according to the protocol of a genome walking kit (Takara, Japan). The sequences of the primers used for amplification are shown in Table S1 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and was converted into cDNA prior to sequencing using the SMARTer technology (SMARTer Pico PCR cDNA Synthesis Kit, Takara, cat. # 634928). Sequencing libraries were prepared using the Illumina TruSeq protocol and sequenced on an Illumina MiSeq using 250 bp paired end reads ...
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bioRxiv - Biophysics 2020Quote: ... coli MG1655 genome by colony PCR and cloned into pR6K-PAmCherry-lox71-cm-lox66 using the In-Fusion® HD cloning kit (Clontech). Mutations were introduced using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) ...
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bioRxiv - Genomics 2020Quote: ... PCRs were performed on board using 25 ng of DNA from each coral sample with the Advantage 2 kit (Takara Clontech) and a final primer concentration of 0.5 μM in a final reaction volume of 50 μl ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was amplified using 8 µl of the PCR MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing, Takara Bio) with 25 cycles of amplification ...
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bioRxiv - Biochemistry 2020Quote: ... The PfNEDD8 PCR product was cloned into the StuI-HindIII digested pSMO plasmid using in-Fusion® HD Cloning Plus kit (Takara) and transformed into DH5α E ...
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bioRxiv - Neuroscience 2021Quote: ... The cloning reaction for pSPE3::H2B-GCaMp6s was performed using linearised PCR products and the In-Fusion HD Cloning Kit (Takara Bio). H2B-GCaMP6s mRNA was produced and precipitated using the mMESSAGE mMACHINE T3 kit (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... single cells were sorted into individual wells of 8-well PCR strips containing lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894) with RNase inhibitor (0.17 U/μl) ...
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bioRxiv - Neuroscience 2020Quote: ... single cells were sorted into individual wells of 8-well PCR strips containing lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894) with RNase inhibitor (0.17 U/μl) ...
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bioRxiv - Plant Biology 2020Quote: ... The LightCycler 480II PCR system was programmed according to the instructions of the SYBR Premix Ex Taq II (Perfect Real Time) kit (TaKaRa, China) to conduct fluorescence-based quantitative PCR ...
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bioRxiv - Synthetic Biology 2021Quote: ... An aliquot of 1 μg of DNAse treated RNA was used for cDNA synthesis using the PrimeScript™ RT-PCR Kit (Takara) in a final volume of 20 μL according to the manufacturer indications ...
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bioRxiv - Cancer Biology 2022Quote: ... Truncations of ENDOD1 and mutations of TP53 were obtained by fusing different PCR fragments using In-Fusion cloning kit (Clontech, 639650). Primers used in this study were listed in Supplementary Table S3.
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bioRxiv - Molecular Biology 2020Quote: ... and hERG mutants were generated by PCR-mediated site-directed mutagenesis using an In-Fusion HD Cloning Kit (Takara, Otsu, Japan) as described previously (Kume et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplicons were isolated from the agarose gel using the PCR cleanup and gel extraction kit (ClonTech, Takara USA, Mountain View, CA) and the nucleotide sequence of amplicons determined by Sanger direct sequencing (GENEWIZ ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplicons were isolated from the agarose gel using the PCR cleanup and gel extraction kit (ClonTech, Takara USA, Mountain View, CA) and the nucleotide sequence of amplicons determined by Sanger direct sequencing (GENEWIZ ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplification on the cleaned libraries was performed as follows: A reaction mix using LA Takara Hot Start kit (Takara Bio) was prepared according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Aliquots of 1 μg of the treated RNA were used for cDNA synthesis with oligo dT using PrimeScript™ RT-PCR kit (Takara). cDNAs (0.4 μl ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of the total RNA from the leaves was reverse-transcribed for 30 min at 42°C in a 20-μl reaction volume using a High Fidelity PrimeScriptTM RT-PCR kit (Takara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... leuC and leuD were measured by qRT-PCR using a SYBR Premix Ex Taq (perfect real time) kit (TaKaRa, Dalian, China). The recA gene was used as an internal control for B ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were purified and fused into a ClaI-digested plasmid with an In-Fusion HD cloning kit (TAKARA, Japan). Mutants for overexpressing HFB2 (TgOPB38530 ...
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bioRxiv - Developmental Biology 2021Quote: ... 350 ng RNA samples in 6 µL nuclease-free water) were generated using the Clontech SMARTer smRNA-Seq kit using 8 PCR cycles (Takara Bio). 30 µL of the PCR reaction was purified with AMPure XP beads (Beckman Coulter A63880 ...
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ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: ... the coding sequence of each gene was amplified from whole larva cDNA using PrimeScript RT-PCR Kit (Takara, cat # RR014-A). The resulting products were recombined into pDONR221 and transferred to the pTWGA vector to finally produced the desired plasmids ...
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bioRxiv - Genetics 2020Quote: ... of which 16 reactions (200 ng in each) were amplified for 29 cycles and purified using Macherey Nagel Nucleospin Gel and PCR Purification kit (Takara Bio). Sequencing libraries were made of the 4C PCR products using Thruplex DNA-seq kit (Takara Bio) ...
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bioRxiv - Genomics 2021Quote: ... reverse transcription and cDNA preamplification were performed in the C1 Fluidigm system using the SMARTer PCR cDNA Synthesis Kit (Clontech 634926) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... were used to transcribe poly (A)-tailed miRNAs to generate a cDNA library according to the PrimeScript™ miRNA RT-PCR Kit (Takara) instructions ...
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bioRxiv - Microbiology 2021Quote: ... First the coding region of Candid #1 RdRp was amplified using RNA extracted from P0 or P11 viruses and the PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio). Kozak sequence ...
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bioRxiv - Cell Biology 2022Quote: ... Donor vectors containing the GFP or mKate2 coding sequence flanked by 1kb homology arms adopted from both 3′ and 5′ sides of the insertion site were generated by cloning of PCR-amplified tags and arms into linearized pGEM-3z vector by In-Fusion HD Cloning Kit (Takara Bio). Obtained gRNA expression plasmids and donor plasmids were injected into the y w embryos with a final concentration of 120 ng/ul for each ...
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bioRxiv - Cell Biology 2022Quote: ... NM_001123007) was amplified from 24 hpf embryo RNA using the PrimeScript II High Fidelity One Step RT-PCR kit (Takara, R026A) and the following primers ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from HT-29 cells using the CellAmp Direct RNA Prep Kit for RT-PCR (3732; TaKaRa, Japan) after the introduction of genomic DNA or Poly (I ...
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bioRxiv - Plant Biology 2022Quote: ... The expression analysis of each target gene was performed quantitatively by real-time RT-PCR method after cDNA synthesis using PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa) and TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Zoology 2024Quote: ... RNA was extracted using Isogen-LS (Nippon Gene) and RT-qPCR was performed using the One-Step PrimeScript RT-PCR Kit (Takara Bio) on a 7500 Real-Time RT-PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... the coding sequence of each gene was amplified from whole larva cDNA using PrimeScript RT-PCR Kit (Takara, cat # RR014-A). The amplified sequence was then purified through gel extraction (Magen HiPure Gel Pure DNA Mini kit ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were analysed by gel electrophoresis and positive products were purified using the NucleoSpin Gel and PCR clean up kit (Clontech, Takara Bio) before being sent for sequencing by Eurofins Genomics (Germany) ...
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bioRxiv - Neuroscience 2023Quote: ... expressing UAS-eGFP was subjected to 14 cycles of PCR amplification (SMARTer Seq V4 Ultra Low Input RNA Kit; Takara Bio). 1 ng of amplified RNA was used to prepare cDNA libraries (Nextera XT DNA library preparation kit ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was further purified using mRNA Dynabeads and the purified mRNA was used as template for synthesis of cDNA using SMARTer PCR cDNA kit (Takara-Bio), with 18 cycles of amplification ...
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bioRxiv - Cell Biology 2023Quote: RACE-ready (Rapid Amplification of cDNA 5’ Ends) cDNA was generated using SMARTer PCR cDNA Synthesis Kit (#634925, Clontech Laboratories, Inc.) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2023Quote: ... purified via gel extraction from a 1% agarose gel followed by cleanup using the NucleoSpin PCR clean up and gel extraction kit (Takara Bio). Linearized CROP-seq-optiI and amplified oligonucleotides were assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB ...
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bioRxiv - Immunology 2023Quote: ... A total of 100 ng isolated RNA was used for cDNA synthesis using PrimeScript High Fidelity RT-PCR Kit (Takara, R022B). RT-qPCR was performed using TB Green® Premix Ex Taq™ (Tli RNase H Plus ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed in a single-step using the One-Step TB Green PrimeScript RT-PCR Kit II (Takara Bio). The HCoV-OC43 nucleocapsid gene was amplified with the following primers ...
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bioRxiv - Microbiology 2023Quote: ... linearized chimeric H3 HA sequence was gel purified using the NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... A quantity of RNA in the range 200 – 500 ng was used for cDNA synthesis using the PrimeScript RT-PCR Kit (Takara-Bio). Quantitative PCR was then performed using Taqman Fast Advanced MasterMix with Universal Probe Library Probes (Roche ...