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Citations for Takara Bio :
701 - 750 of 6736 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: A cDNA fragment of Gtsf1L was amplified by RT-PCR from BmN4 total RNAs and cloned into pIZ vector by In-fusion cloning kit (Takara).
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bioRxiv - Evolutionary Biology 2022Quote: ... The cDNA samples were cloned into a pUC19 vector (PCR-amplified with primers 11 and 12) using In-Fusion HD Cloning Kit (Takara). After transformation into Escherichia coli ...
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bioRxiv - Molecular Biology 2023Quote: ... The ORF of zebrafish znf598 (ENSDARG00000014945) was amplified by RT-PCR and cloned into pCS2+ via XhoI/XbaI restriction sites using DNA Ligation Kit (TAKARA). To generate znf598 point mutation in ORF (znf598 C13/16A) ...
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bioRxiv - Molecular Biology 2023Quote: ... The ORF of zebrafish rpl36 (ENSDARG00000100588) was amplified by RT-PCR and cloned into pCS2+ via EcoRI/XhoI restriction sites using DNA Ligation Kit (TAKARA). The ORF of zebrafish znf598 (ENSDARG00000014945 ...
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bioRxiv - Neuroscience 2022Quote: ... the BamHI/BsrGI digested PCR product was ligated to the linearized backbone using the DNA Ligation Kit - Mighty Mix (TaKaRa) resulting pPB-ef1a-NLS-EGFP-T2A-puro.
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bioRxiv - Molecular Biology 2022Quote: ... Probes for FLuc (500 bp) were generated using gel-purified PCR amplicons containing GFP sequence and a BcaBEST Labeling kit (Takara) and [α-32P]-dCTP (PerkinElmer) ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA encoding ZP3 was restored from the mouse ovarian tissue by RT-PCR using PrimeScript RT Reagent Kit (Takara, RR037). Two PCR amplicons including the 5′ region of the TECTA-ZP and the transmembrane domain (TMD ...
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bioRxiv - Immunology 2023Quote: ... Each gene fragment was PCR-amplified and cloned into a pcDNA 3.1 vector by using the In-Fusion HD cloning kit (Takara Bio). Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were then reverse transcribed employing a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan). Nested PCR conditions were as follows ...
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bioRxiv - Neuroscience 2024Quote: ... and then the tip of the recording electrode was broken into the nested PCR reagent system (Prime ScriptTm RT reagent Kit with QDNA Eraser, Cat: RR0471, Takara), and a cDNA synthesis kit was used to synthesize cDNA following the manufacturer’s protocol (PrimeScriptm II1st Strand cDNA Synthesis Kit) ...
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bioRxiv - Cell Biology 2024Quote: A PCR product containing the Human ORF for DDX3X was obtained directly from RNA using the Primescript High Fidelity RT-PCR kit from Takara. The PCR primers introduced BamHI and NotI sites at the 5’ and 3’ ends respectively ...
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bioRxiv - Genomics 2023Quote: ... Digests were run on a 0.8% agarose-TAE gel and full-length plasmids were extracted with the Nucleospin PCR and Gel Cleanup Kit (Takara, 740609). A LR reaction was performed with 150 ng entry library and 150 ng pDEST_HC_Rec_Bxb_v2 ...
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bioRxiv - Microbiology 2023Quote: ... The resultant reaction mixture was then 10-fold diluted and subjected to quantitative PCR using TB Green Premix Ex Taq II kit (TaKaRa) in combination with a tag primer (no ...
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bioRxiv - Cancer Biology 2023Quote: ... the agarose gel pieces were cut out and digested using NucleoSpin® Gel and PCR Clean-Up kit (Takara, 740609), according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... generated cDNA was subjected to real-time PCR analysis using SYBR® Premix Ex Taq™ II kit (TAKARA BIO) with the sets of specific primers (Table 1) ...
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bioRxiv - Genetics 2023Quote: ... Reverse transcription was performed using the oligo (dT) primer and AMV reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). The RT-PCR was performed using KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Microbiology 2024Quote: ... Then the total RNA was extracted and reversed transcribed into cDNA for quantitative PCR by using the PrimeScript RT Reagent Kit (TaKaRa). Quantitative real-time reverse transcriptase PCR (qRT-PCR ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of unpurified RT product was amplified in 12.5 µl using the Advantage HF 2 PCR Kit (Takara Bio) with 1X Advantage 2 PCR Buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 4F2hc and mZIP13 or mZIP14 were fused by PCR and In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... in conjunction with the Mir-X™ miRNA qRT-PCR TB Green® Kit from TAKARA (San Jose, CA, USA). After confirming miRNA inhibition in tick tissues ...
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bioRxiv - Cell Biology 2024Quote: ... the RNA was reverse transcribed into complementary DNA by PrimeScript RT (reverse transcriptase) using a primeScript RT-PCR kit (Takara), and qPCR was performed with SYBR Green-Master Mix (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... AAV titer was determined by qPCR method using AAVpro Titration Kit (for Real Time PCR) Ver.2 (Takara-bio, #6233) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: cfDNA in the conditioned cell culture medium was extracted using NucleoSpin Gel and PCR Clean-up kit (Takara, Duren, Germany), and quantified with Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... The RNA copies of SARS-CoV-2 was quantified by RT-qPCR targeting the S gene using the One Step PrimeScript RT-PCR Kit (RR057B, Takara) with specific primers and probes ...
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bioRxiv - Immunology 2024Quote: ... or from sort-purified CD11c+/- splenocytes from Smc3ΔDC mice (for confirming recombination of floxed Smc3 allele by PCR) using the NucleoSpin Tissue kit (Takara Bio) according to the manufacturer protocol.
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bioRxiv - Synthetic Biology 2024Quote: ... Nine sequence-modified pET-aaRSs were prepared by PCR amplification followed by self-ligation using a mutated and In-Fusion cloning Kit (TaKaRa). The circular DNA encoding the phi29 DNA polymerase used in the aaRS activity assay (Fig ...
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bioRxiv - Genetics 2024Quote: ... The DNA was purified and concentrated from agarose gels using the Nucleospin Gel and PCR Clean-up kit (Takara Bio) and samples were normalized to 1ng/μl using a Qubit fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR amplification was carried out using EmeraldAmp GT PCR Master Mix (Clontech, Takara) according to the manufacturer’s protocol and the primers shown in Table S1 and Figure 2 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR amplification was carried out using EmeraldAmp GT PCR Master Mix (Clontech, Takara) according to the manufacturer’s protocol and the primers shown in Table S1 and Figure 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR product was purified with NucleoSpin Gel and PCR Clean-up (Takara) and digested with AsiSI at 37 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... pneumophila (RefSeq NC_002942.5) genomic DNA by PCR using CloneAmp HiFi PCR premix (Takara) and the relevant primers ...
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bioRxiv - Systems Biology 2021Quote: ... all PCRs utilized a high fidelity polymerase (CloneAmp HiFi PCR Premix, Takara 639298). Plasmids contained components of the synthetic inducible system flanked by homology to the intended genomic integration locus and were restricted prior to transformation ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR mixture contains 5 ul EmeraldAmp GT PCR Master Mix (Takara, #RR310B), 1 ul genomic DNA ...
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bioRxiv - Genomics 2022Quote: We performed PCR with CloneAmp HiFi PCR Mix (Takara Biosciences, Mountain View, CA) and added DMSO to a final concentration of 5-10% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... semiquantitative PCR was performed using 2X EmeraldAmp GT PCR Master Mix (Takara-#RR310) and gene-specific primers and the amplified PCR products were visualized on 2% agarose gel ...
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bioRxiv - Neuroscience 2023Quote: PCR amplifications for cloning were performed with the CloneAmp HiFi PCR Premix (Clontech) and plasmid fusion/reassembly were performed with the NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR was performed using SapphireAmp® Fast PCR Master Mix (Takara; RR350A), while iTaq™ Universal SYBR® Green Supermix (Biorad ...
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bioRxiv - Microbiology 2023Quote: ... a PCR test is performed using the Terra PCR Direct Polymerase Mix (Takara Bio Europe SAS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... PCR was then performed using SYBR Green PCR Master Mix (Takara, Kyoto, Japan) for 40 cycles (95°C for 3 sec and 60°C for 30 sec) ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus was collected after 24 hours for 3 consecutive days and concentrated with Lenti-X Concentrator (Takara Bio USA, Mountain View, CA). A mixture of two shRNA constructs for SPCA2 was used ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus was collected after 24 hours for 3 consecutive days and concentrated with Lenti-X Concentrator (Takara Bio USA, Mountain View, CA). A mixture of two shRNA constructs for SPCA2 was used ...
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bioRxiv - Cancer Biology 2023Quote: Production of vesicular stomatitis virus (VSV-G) pseudotyped lentivirus was performed by calcium phosphate transfection of Lenti-XTM 293T cells (TaKaRa Clontech, 632180) with CROP-mCherry and helper plasmids pMD2.G and psPAX2 (Addgene plasmids 12259 and 12260) ...
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bioRxiv - Genomics 2024Quote: Production of vesicular stomatitis virus (VSV-G) pseudotyped lentivirus was performed by calcium phosphate transfection of Lenti-X™ 293T cells (TaKaRa Clontech, 632180) using the CROP-mCherry-Fos construct and the helper plasmids pMD2.G and psPAX2 (Addgene plasmids 12259 and 12260) ...
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bioRxiv - Bioengineering 2022Quote: ... PCR was performed in the TaKaRa PCR Thermal Cycler Dice Touch (TaKaRa, Shiga, Japan) using the Tks Gflex DNA Polymerase (TaKaRa) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was conducted using a Takara PCR thermal cycler Dice mini (Takara Bio Inc.) as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were purified with NucleoSpin Gel and PCR Clean-Up (Takara Bio, #740609). 400 ng of PCR products mixed with NEB buffer 2 were denatured at 95°C for 5 minutes and ramped down to 25°C ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative PCR experiments were performed using SYBR Green PCR Master Mix (Takara, Otsu, Japan) with Bio-Rad CFX96 real-time PCR machine ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary PCR was performed using EmeraldAmp® GT PCR Master Mix (Takara cat# RR310A) 10 ul ...
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bioRxiv - Microbiology 2020Quote: ... Each PCR reaction contained 2.5 µl of PCR buffer (TaKaRa, 10x concentration, 1x final), 1 µl of the Golay barcode tagged forward primer (10 µM concentration ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-up (TaKara Bio Inc.), and then analyzed and quantified by Nanodrop spectrometer (Thermo Fisher Scientific ...