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Citations for Takara Bio :
1001 - 1050 of 6736 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... washed with 200 µL water, and resuspended in PCR mix (22 µL water, 25 µL Terra PCR direct buffer, 1 µL Terra Polymerase (Takara), 1 µL 100 M Truseq PCR handle primer ...
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bioRxiv - Cell Biology 2022Quote: All plasmids construction was performed by PCR amplification (CloneAmp HiFi PCR Premix) using appropriate primers followed by Gibson assembly (In-Fusion HD Cloning system, Clontech) into the EcoRI site of pLVX-puro vector (Clontech ...
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bioRxiv - Molecular Biology 2021Quote: ... A 150-250 bp region encompassing each targeted locus was PCR amplified from ∼40 ng genomic DNA with ends containing partial Illumina adapter sequences using CloneAmp HiFi PCR (Takara). Reaction conditions were as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... linearized by PCR and using the divergent primers reported in Supplementary Table 3 and CloneAmp™ HiFi PCR Premix (Takara) polymerase ...
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bioRxiv - Developmental Biology 2024Quote: ... Equimolar amounts of PCR products were mixed and a PCR was made with a primeSTAR GXL DNA polymerase (Takara Bio) without primers for the first 10 cycles using the following thermocycler conditions (95°C 3min ...
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bioRxiv - Molecular Biology 2022Quote: Two hundred nanogram of DNAs were used as a template for polymerase chain reaction (PCR) by using EmeraldAmp MAX PCR mater mix (Takara). First 30 cycles of PCR were performed using forward and reverse primers below ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on three biological replicates using the Thermal Cycler Dice Real Time System TP-760 (Takara) with the Kapa SYBR fast universal qPCR kit (Kapa Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... The deletion of each Stx2a phage in the CmR transformants was confirmed by colony PCR using EmeraldAmp MAX PCR Master Mix (TaKaRa) and specific primers ...
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Mutations in HUA2 restore flowering in the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutantbioRxiv - Plant Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using TB Green™ Premix Ex Taq™ II (Takara, Dalian, China). Relative gene expression was calculated using the 2−ΔΔCt method (Rao et al. ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was amplified by PCR by adding 1 µl of the Terra PCR Direct Polymerase (1.25 U/µl, Takara 639270), 25 µl of the Terra PCR Direct buffer and 1 µl of the ISPCR primer (10 µM stock concentration ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplification with Gateway cloning compatible primers was performed directly on Aag2 gDNA using CloneAmp Hifi PCR pre-mix (Takara), without prior amplification in a TOPO TA cloning vector ...
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bioRxiv - Plant Biology 2022Quote: ... were chemically synthesised and cloned by PCR into the pJET1.2 based gRNA vectors using Clontech CloneAmp HiFi PCR Premix (Takara, Japan). The transformation vector pICU2:Cas9-dsRED containing the Cas9 gene from Streptococcus pyogenes expressed under the promoter of the Arabidopsis Incurvata 2 gene (ICU2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The construct containing Y181G-coding point mutation was generated by inverse PCR of Zdhhc20WT plasmid using CloneAmp HiFi PCR Premix (Takara) and joining the ends of the PCR product using InFusion cloning.
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR products of the exons were assembled into one full-length sequence using overlapping PCR (In-fusion cloning, Clontech) for each TAS1R and were then subcloned into the pEAK10 expression vector (Edge Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) reactions were performed using TB Green Premix Ex Taq TM II (Tli RNaseH Plus; Takara) on a Light Cycler 480 II (Roche Diagnostics) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative genomic real-time PCR (gRT-PCR) reaction was performed using TB Green Premix Ex Taq TM II (Tli RNaseH Plus; Takara) following standard procedures ...
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bioRxiv - Developmental Biology 2024Quote: ... CRISPR-targeted regions in marcks.L/S and marcksl1.L/S were PCR-amplified using the EmeraldAmp GT PCR Master Mix (Takara Bio). For every gene ...
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bioRxiv - Developmental Biology 2024Quote: ... and 5 LR cells) were individually re-amplified using the 5’ PCR Primer II A with CloneAmp HiFi PCR Premix (Clontech Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... was performed via standard and overlapping PCRs with CloneAmp™ HiFi PCR Premix (Takara Bio, cat # 639298, San Jose, CA), with subsequent insertion into the construct at unique restriction sites by In-Fusion ligation- independent cloning (Takara Bio ...
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bioRxiv - Genomics 2024Quote: ... 1X Terra PCR Direct Buffer and 1 uL of 1.25 U/uL Terra PCR Direct Polymerase Mix (639270, Takara Bio). To clean up the reaction ...
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bioRxiv - Developmental Biology 2020Quote: ... were transferred into 0.2-mL PCR tubes and processed for sequencing using Seq® v4 Ultra® Low Input RNA Kit (Takara Bio USA, Mountain View, CA) as described in Wood et al ...
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bioRxiv - Biophysics 2023Quote: Plasmids used in this study (Supplementary Table S1) were assembled by Polymerase Chain Reactions (PCR) amplifying insert and vector DNA fragments followed by In-Fusion cloning (Takara Biosciences, In-Fusion HD Cloning Kit). Oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... using SYBR Green PCR Master Mix (Takara, Japan). The relative expression levels of the target gene (EGFP ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR fragments were cloned into pLVX-puro (Clontech) lentivirus vector with Gibson Assembly Mixture (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... and PCR was performed using PrimeSTAR GXL (Takara) with pCAGItdTomato-Rpsa ...
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bioRxiv - Microbiology 2021Quote: ... and analyzed by PCR using PrimeSTAR (TaKaRa, R040A). The primers used for monitoring the efficiency of vFLIP knockout are Pr1 (ACCCTGCGTAAACAACCG ...
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bioRxiv - Cancer Biology 2022Quote: ... using the SYBR green PCR master mix (Takara). Reactions were performed in duplicate or triplicate ...
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bioRxiv - Plant Biology 2021Quote: ... by PCR using PrimeSTAR GXL DNA Polymerase (TaKaRa) and the primer set RenLuc-pPLV26-F/R (Supplementary Table S11) ...
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bioRxiv - Microbiology 2021Quote: ... was amplified using CloneAmp HiFi PCR mix (Takara) in a 2-step PCR-program of 98°C for 10 seconds followed by 68°C for 4 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... or by CloneAmp HiFi PCR Premix (Takara Bio). Random mutagenesis was performed using Error-Prone PCR (EP-PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... and Nucleospin Gel and PCR Clean-up (Takara). The sequencing library was sequenced with the Illumina Next-Seq 500 instrument (Illumina ...
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bioRxiv - Genetics 2021Quote: ... PCR was performed using PrimeSTAR HS (Takara Bio) and T100 Thermal Cycler (Bio-rad ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were digested with DpnI (TaKaRa) at 37°C for 1 h ...
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bioRxiv - Genetics 2020Quote: ... PCR products amplified using LA Taq polymerase (Takara) or Multiplex PCR Kit (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... Mutagenic PCR reactions used PrimeStar GXL polymerase (Takara), and 45mer mutagenesis primers (Table S2 ...
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bioRxiv - Cell Biology 2020Quote: ... EGFP was PCR-amplified from pEGFP-C2 (Clontech) and similarly cloned into the pRSET His-SS-TEV vector ...
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bioRxiv - Immunology 2020Quote: ... The riboswitch was amplified by PCR (CloneAmp, Takara; according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Using PCR and In-Fusion cloning (Takara Bio), the ‘NGG’ PAM-recognition domain of the prime editor protein (PE2 ...
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bioRxiv - Microbiology 2023Quote: ... 1x of PCR buffer (10x, Takara, CA, USA), 10 μL of barcoded primer set forward/ reverse from ONT ...
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bioRxiv - Microbiology 2023Quote: ... In the In-Fusion PCR cloning system (Clontech), the S1 or S2 gene of the HV80 strain was replaced with the corresponding region of the H120 strain to construct the recombinant plasmids pMDVM8-HV80S1H120S2 and pMDVM8-H120S1HV80S2 ...
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bioRxiv - Genomics 2023Quote: ... PCR was performed using Taq polymerase (TAKR001C, ClonTech) when running DNA fragments on a gel ...
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bioRxiv - Bioengineering 2023Quote: For PCR reactions PrimeSTAR HS DNA Polymerase (Clontech), Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... using CloneAmp™ HiFi PCR Premix (Takara 639298) and cloned it (in frame ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification was conducted using PrimeSTAR Max (Takara) or KOD-Plus-Neo (TOYOBO).
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bioRxiv - Cell Biology 2023Quote: ... using a Takara Thermal PCR Cycler (Takara Bio). RT-PCR of miRNAs was performed with a Taqman microRNA Reverse Transcription Kit (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... PCR amplification (PrimeSTAR Max polymerase, Takara Bio USA), and In-Fusion cloning (In-Fusion Snap Assembly Master Mix ...
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bioRxiv - Pathology 2024Quote: ... SYBR Green PCR reagent (Takara Co. Ltd., Japan) was chosen for real-time fluorescence quantification ...
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bioRxiv - Immunology 2024Quote: ... PCR was performed using Titanium Taq (Takara, #639208) with an extension time of 90s for 30 (1st round ...
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bioRxiv - Molecular Biology 2023Quote: ... All PCR analyses were performed using PrimeStarGXL (TaKaRa) as follows ...
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bioRxiv - Microbiology 2022Quote: ... PCR was performed with Ex-Taq enzyme (TaKaRa) in a total volume of 30 µl containing 1 µl viral DNA ...