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Citations for Takara Bio :
601 - 650 of 1429 citations for BFF 122 CAS 1152314 49 2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... and then cloned into corresponding entry vectors using In-Fusion cloning (Takara Bio, San Jose, CA, USA) or a one-step Gibson reaction with the in-house master mix ...
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bioRxiv - Cell Biology 2022Quote: ... and 5 units of SMART Moloney murine leukemia virus reverse transcriptase (Takara Bio, Mountain View, CA, USA). The RT reaction was carried out at 42°C for 2 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... The extract was incubated for 1 h at 4 °C with Talon resin (Clontech, Mountain View, CA), loaded onto a column ...
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bioRxiv - Cell Biology 2022Quote: ... the respective fragments were excised with EcoRI and ligated into pCMV-Myc (Clontech Laboratories, Mountain View, CA). To generate the constructs coding for the double tagged Sun4 peptide sequences ...
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bioRxiv - Molecular Biology 2020Quote: The cDNA synthesis kits and SYBR Green Master Mix were obtained from Clontech (Mountain View, CA, USA). Dexamethasone and Granzyme B inhibitor Z-AAD-CMKwere purchased from Sigma-Aldrich (St ...
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bioRxiv - Cancer Biology 2020Quote: ... GFP positive cells were selected by FACS sorting after tetracycline (Takara Bio Inc., San Francisco, CA, USA) treatment (1µg/ml) ...
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bioRxiv - Developmental Biology 2022Quote: ... Virus containing supernatant was collected and virus particles were concentrated by Lenti-X concentrator (Clontech Laboratories, CA) according to the manufacturer protocol ...
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bioRxiv - Systems Biology 2022Quote: ... cDNA was generated using the SMART-Seq V4 Ultra Low RNA Kit (Takara Bio, Mountain View, CA). After 14 cycles of library amplification ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were incubated with rabbit anti-DsRed antibody (1:1,000; #632496, Takara Bio., Mountain View, CA) at room temperature overnight ...
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bioRxiv - Immunology 2022Quote: ... RNA (1 ng) was amplified using SMARTer library Ultra Low cDNA v4 kit (Takara Bio, Mountainview, CA). Sequencing cDNA libraries were preparing using a NexteraXT DNA library prep kit (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: KPC cells were transduced with either an empty vector (EV) pLVX-IRES-mCherry (Clontech, Mountain View, CA) or the same backbone vector expressing the human K17 open reading frame cDNA ...
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bioRxiv - Genetics 2019Quote: ... and specific bands were cleaned using NucleoSpin gel clean-up kit (Takara Bio, Mountain View, CA, U.S.A.) for subsequent Sanger sequencing at UMGC ...
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bioRxiv - Cell Biology 2020Quote: ... This step was performed using commercially available In-Fusion HD Cloning Kit (Clontech, Mountain View, CA, USA). The produced plasmid expresses PRC1 tagged with tgRFPt and SspB at the C-terminus ...
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bioRxiv - Developmental Biology 2020Quote: ... tetracycline-controlled transactivator (tTA) and the SV40 large T antigen polyadenylation signal (Takara Bio, Mountain View, CA), into B6SJLF1 fertilized eggs ...
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bioRxiv - Bioengineering 2020Quote: ... Plasmid assembling steps were performed with the In-Fusion HD Cloning Kit (Clontech, Mountain View, CA, USA). The gene cassettes used for the cell-surface expression of hemicellulases were previously optimized [43 ...
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bioRxiv - Biochemistry 2019Quote: ... The amplified fragments were ligated using an In-Fusion HD cloning kit (Clontech, Mountain View, CA, USA). pcDNA3.1(-)P-gp(MM ...
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bioRxiv - Molecular Biology 2020Quote: ... Reverse PCRs were performed in two rounds using CloneAmp HiFi PCR premix (Clontech, Mountain View, CA, USA). The first round was performed adding the forward and reverse primers in two independent reactions and consisted of 3 cycles of 98 °C for 10 seconds ...
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bioRxiv - Microbiology 2021Quote: Routine cloning was performed using In-Fusion HD Cloning Plus (Takara Bio USA Inc., Mountain View, CA) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... and 5 units of SMART Moloney murine leukemia virus reverse transcriptase (Takara Bio, Mountain View, CA, USA). The RT reaction was carried out at 42°C for 2 hrs ...
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bioRxiv - Biophysics 2019Quote: ... Excess primers were removed from the PCR products using NucleoSpin silica-membrane columns (Clontech, Mountain View, CA) and the products were further purified in 3% agarose-TBE gels and subsequently reisolated using gel-purification kits (Clontech).
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bioRxiv - Cell Biology 2019Quote: ... followed by in-frame cloning into XhoI-ApaI sites of pEYFP-N1 (Clontech, Palo Alto, CA, USA). RNF167 WT and all the variants were cloned into pMYs-IRES-Puro (Cell Biolabs ...
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bioRxiv - Immunology 2021Quote: ... The baculovirus master and working stock titers were determined using rapid titer kit (Clontech, Mountain View, CA). Recombinant baculovirus stocks were prepared by infecting Sf9 cells with a multiplicity of infection (MOI ...
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bioRxiv - Cell Biology 2020Quote: ... SMART-Seq V4 Ultra Low RNA Input Kit for Sequencing (Takara Bio USA, Inc., Mountain View, CA) was used for reverse transcription and generation of double stranded cDNA for subsequent library preparation using the Nextera XT Library Preparation kit (Illumina ...
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bioRxiv - Genomics 2020Quote: cDNA was synthesized from parasite RNA using the SMARTer PCR cDNA synthesis kit (Takara, Mountain View, CA), which incorporates a poly(A ...
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bioRxiv - Microbiology 2021Quote: ... We used the Prime Star high-fidelity Taq polymerase and buffer (Takara Bio USA, Mountain View, CA) with 50ng of template DNA and the following thermocycler settings ...
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bioRxiv - Microbiology 2020Quote: ... The baculovirus master and working stock titers were determined using rapid titer kit (Clontech, Mountain View, CA). Recombinant baculovirus stocks were prepared by infectingSf9 cells with a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2021Quote: The RNA was converted to cDNA with the Clontech SMARTer kit (Takara Bio USA; Mountain View, CA) using the modified (Switching Mechanism At 5’ End of RNA Transcript ...
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bioRxiv - Plant Biology 2022Quote: The PsiYAB11 and PtoYAB11PSC genes were cloned into the pGADT7-rec vector (Clontech, Mountain View, CA, USA) with the GAL4 activation domain ...
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bioRxiv - Synthetic Biology 2022Quote: ... digested with appropriate restriction enzymes utilizing In-Fusion PCR cloning technology (Clontech Laboratories, Inc., Mountain View, CA). Cloning inserts were created via PCR of ORFs of interest from their respective genomic (all E ...
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bioRxiv - Microbiology 2023Quote: ... LASVpp-BlaM viruses were concentrated ten times with Lenti- X concentrator (Clontech Laboratories, Mountain View, CA, USA), snap-frozen and stored at - 80 °C.
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bioRxiv - Molecular Biology 2022Quote: ... rAAVs were purified using AAVpro® Purification Kit for All Serotypes (Cat # 6666, Takara Bio USA, CA) and each of the purified rAAVs were tested in separate transwell BBB cultures with hCMEC/D3 cells and primary astrocytes at MOI of 1e4 ...
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bioRxiv - Neuroscience 2024Quote: ... Human KCNQ2 cDNA (GenBank accession number NM_172108) in pIRES2-EGFP (BD Biosciences-Clontech, Mountain View, CA, USA) was used as template for in vitro mutagenesis ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: ... mRNA was converted to cDNA using SMARTer v4 Ultra Low Input RNA Kit (Clontech, Mountain View, CA). A Diagenode Bioruptor Pico was used to fragment the cDNA ...
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bioRxiv - Microbiology 2024Quote: ... Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Genomics 2024Quote: ... Both reagents were included in SMART-Seq Single Cell PLUS kit (Takara Bio, San Jose, CA, U.S.). Immediately after sampling in lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were prepared using the SMARTer v4 Ultra Low Input RNA Kit (Clontech, Mountain View, CA). Sequencing was performed on NovaSeq S4 150PE platform (Illumina ...
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bioRxiv - Genomics 2024Quote: ... The PCR reaction mix consisted of 1.25 PrimeSTAR GXL DNA Polymerase (Takara Bio USA, San Jose, CA), 1× Buffer ...
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bioRxiv - Bioengineering 2024Quote: ... All proteins were purified with a Talon metal affinity resin (Takara Bio USA, Mountain View, CA, USA) and dialyzed against pyrogen-free PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... A431 epithelial carcinoma cells (ATCC; Manassas, VA) and GP2-293 cells (Takara Bio USA; San Jose, CA) were cultured in high glucose DME ...
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bioRxiv - Cell Biology 2022Quote: ... cells were diluted to 25,000 cells/mL and dispensed into ICELL8 3’ DE chips (Takara Bio, CA) using an MSND device (Takara Bio) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HepG2 cells were resuspended in ice-cold PBS supplemented with protease inhibitors (Clontech, Mountain View, CA, USA) and lysed in ice bath by sonication in cycles of 10 s on /5 s off for 1 min at 6–10 μm amplitude at 25 % intensity from an exponential ultrasonic horn of 3 mm in a Soniprep 150 MSE (MSE Ltd. ...
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Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the supernatant was loaded onto a 5 ml column with prewashed Talon resin (Clontech Laboratories, Inc, CA). The column was washed three times with the low pH buffer (pH 6.3) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1-st PCR (PCR 1) was performed using Titanium Taq DNA Polymerase (# 639209, Takara Bio, CA, USA). Separation of the PCR products from primers and gel purification was done by QIAquick PCR & Gel Cleanup Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells were transiently transfected with the expression control vector pCMV-TRE3G (Clontech, Mountain View, CA, USA) and the coexpression construct pTRE3G-BI/GPR83-LgBiT:SmBiT-ARRB2 that encodes a C-terminally LgBiT-fused human GPR83 (LgBiT-GPR83 ...
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bioRxiv - Immunology 2023Quote: ... viral particles were concentrated 20-fold to 400Lμl using Lenti-X Concentrator (Clontech, #631231, Mountain View, CA), and 100Lμl of concentrated virus was used to infect cells ...
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bioRxiv - Biochemistry 2023Quote: ... and hSTEAP2 was purified from the supernatant using cobalt-based Talon affinity resin (Clontech, Mountain View, CA). The C-terminal His6-tag was cleaved with tobacco etch virus (TEV ...
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bioRxiv - Neuroscience 2023Quote: Fhod3 cDNA was amplified with PrimeSTAR® GXL DNA Polymerase (R050B; Takara Bio, San Jose, CA, USA) using a pair of primers (5’-CACCATGGCCACGCTGGCTTGTCGCGTGCAG3’ for the forward and 5’-GAGCATGCTCACAGTTGCAGTTCAGATGTG −3’ for the reverse primer) ...
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bioRxiv - Genetics 2024Quote: ... 1–1249 (full-length) were cloned into an EGFPC1 plasmid (N-terminal GFP tag) (Takara Bio, CA). For non-cleavable INF2 ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was isolated through a NucleoSpin® RNA/Protein kit (Takara Bio USA Inc. San Jose, CA) while heart issue was processed with a RNeasy Fibrous Mini kit (Qiagen ...