Labshake search
Citations for Takara Bio :
701 - 750 of 1429 citations for BFF 122 CAS 1152314 49 2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... VPS33A and VPS33B macronuclear ORFs were PCR-amplified and inserted by In-Fusion cloning (Clontech, Mountain View, CA) in the linearized p6c-myc-Chx vector at the SpeI site ...
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bioRxiv - Cancer Biology 2021Quote: ... the cDNA sequence of ERK5 isoforms were cloned into pLVX-Puro plasmid (Clontech Laboratories, Mountain View, CA, USA). Plasmids were sequenced to confirm the presence of correct cDNA sequences at the 3’-end of the CMV promoter (data not shown) ...
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Airway Gene-Expression Classifiers for Respiratory Syncytial Virus (RSV) Disease Severity in InfantsbioRxiv - Immunology 2020Quote: ... 1 ng of total RNA was amplified using the SMARter Ultra Low amplification kit (Clontech, Mountain View, CA) and libraries were constructed using the NexteraXT library kit (Illumina ...
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bioRxiv - Cell Biology 2019Quote: We created HeLa cells that stably over-express an empty pmCherry vector control (Takara Bio, Mountain View, CA), a monomeric red fluorescent protein variant (ex = 587 nm ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was performed using a PrimeScript™ RT Reagent Kit (Takara Bio, Mountain View, CA 94043 USA). qPCR was performed with gene specific primers and SYBR Green (iTaq Universal SYBR Green Supermix ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Both missing ends could be recovered by RACE PCR (SMARTer RACE kit, Takara Bio, Mountain View, CA, USA).
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bioRxiv - Neuroscience 2021Quote: ... The transfection efficiency of HEK293 cells was evaluated by transfecting cells with EGFP-N1 (Clontech; Mountain View, CA) in parallel reactions ...
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bioRxiv - Plant Biology 2022Quote: ... Purification of His-tagged proteins was carried out using His-tag affinity resin (His-resin) (Clontech, CA, USA).
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bioRxiv - Evolutionary Biology 2021Quote: ... cDNAs were synthesized using the SMARTer RACE cDNA Amplification Kit (Cat. No. 634858; Takara, Mountain View, CA, USA). In silico predicted pou-iv gene sequence was retrieved from the Joint Genome Institute genome database (Nematostella vectensis v1.0 ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products then were inserted into a Tet-on inducible lentiviral vector pLVX (Takara, Mountain View, CA) through EcoRI and BamHI restriction sites ...
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bioRxiv - Immunology 2021Quote: ... Full-length cDNA was generated using the Clontech SMARTer v4 kit (Takara Bio USA, Inc., Mountain View, CA) prior to library generation with the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Immunology 2020Quote: ... The clarified extract was loaded onto a column containing TALON Metal Affinity Resin (BD Clontech, Mountain View, CA) according to the manufacturer’s instructions ...
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A bacterial derived plant- mimicking cytokinin hormone regulates social behaviour in a rice pathogenbioRxiv - Microbiology 2021Quote: ... 5 μg of total RNA was reverse transcribed into cDNA using EcoDryTM Premix (Clontech, Mountain View, CA, USA) according to the manufacturer’s instructions using random hexamer primers ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA libraries were prepared using SMARTer kit (Clontech Laboratories, Inc. A Takara Bio Company, Mountain View, CA, USA) followed by the Nextera protocols (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... Cloning was carried out using the ligation-independent In-Fusion® HD cloning kit (Takara Bio USA, CA). Lentivirus production and transduction were done as described 33 ...
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bioRxiv - Molecular Biology 2022Quote: ... Sequence-verified mutated ORFs were inserted into the pLNCX2 retroviral vector (Clontech Takara, Mountain View CA 94043, USA). The siRNA target sequences were used to generate 64mer DNA oligonucleotides in accordance with the Oligoengine template design (www.oligoengine.com) ...
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bioRxiv - Molecular Biology 2022Quote: ... Sequence-verified mutated ORFs were inserted into the pLNCX2 retroviral vector (Clontech Takara, Mountain View CA 94043, USA). The siRNA target sequences were used to generate 64mer DNA oligonucleotides in accordance with the Oligoengine template design (www.oligoengine.com) ...
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bioRxiv - Neuroscience 2022Quote: ... or a rabbit anti-DsRed polyclonal antibody (1:1000, Cat # 632496, RRID:AB_10013483, Takara Bio U.S.A., Inc., CA, U.S.A.). In addition ...
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bioRxiv - Neuroscience 2022Quote: ... The transfection efficiency of 50B11 cells was evaluated by transfecting cells with EGFP-N1 (Clontech; Mountain View, CA) in parallel reactions ...
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bioRxiv - Microbiology 2024Quote: ... and the protein was purified using cobalt affinity resin (1mL/L culture, Takara Bio USA, San Jose, CA). The protein was washed with 100 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... EGFP and mCherry tagged cDNA constructs were cloned into pBMN and modified pLXIN (Clontech, Mountain View, CA, USA) retroviral expression vectors(Peden et al. ...
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bioRxiv - Bioengineering 2024Quote: ... GFP was detected with a monoclonal mouse anti-GFP antibody (JL-8, Takara Bio USA, San Jose, CA) in Western blot analyses.
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bioRxiv - Neuroscience 2023Quote: ... the following primary antibodies were used: rabbit anti-dsRed (1:1,000, Cat.# 632496, Clontech Laboratories, Mountain View, CA), chicken anti-GFP (1:10,000 ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was applied to a TALON® metal affinity resin column (Takara Bio, Mountain View, CA, USA), and the column was washed with 10 column volumes of wash buffer (50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2023Quote: ... Polyadenylated RNA-seq libraries were prepared by using SMART-Seq v4 PLUS Kit (Clontech, Mountain View, CA, USA). Indexed RNA-seq libraries were sequenced using Illumina HiSeqX sequencer (paired end ...
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bioRxiv - Cell Biology 2023Quote: ... Jerusalem) (73) was subcloned into the XhoI-BamHI sites of the pEGFP-C1 vector (Clontech, Mountain View, CA), and the corresponding SB203580-resistant mutant (T106M ...
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bioRxiv - Microbiology 2023Quote: ... 5’RACE was performed with SMARTer RACE 5’/3’ Kit (Takara Bio USA, Inc. San Jose, CA USA) according to the manufacturer’s directions.
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was isolated from tail biopsies using the NucleoSpin Tissue kit (TaKaRa Bio USA, San Jose, CA) following manufacture protocol ...
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bioRxiv - Neuroscience 2024Quote: ... and 3 μg of RNA was reverse transcribed using PrimeScript™ RT reagent kit (Takara, San Jose, CA), according to manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2021Quote: ... Single embryos were collected in 2 µl RNase free water with Recombinant RNase inhibitor (TAKARA) and ruptured with an RNase free needle ...
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bioRxiv - Evolutionary Biology 2020Quote: PCR amplification was carried out using the Advantage GC 2 PCR Kit (Clontech Catalog #639119), and cloning was carried out using standard procedures (primer sequences are listed below).
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bioRxiv - Biophysics 2019Quote: ... The supernatant was incubated with 2 mL TALON resin (Takara, Saint-Germain-en-Laye, France) in a tube using a rotation shaker for 30-60 minutes ...
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bioRxiv - Genomics 2021Quote: ... and 2) the reduction of the amount of PrimeStar GXL DNA Polymerase kit (Takara, Japan) from 2 ul to 1 ul per reaction.
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bioRxiv - Plant Biology 2021Quote: ... Transformants were selected by cultivation for 2 days on minimal synthetic defined (SD) media (Clontech) lacking Leu (pACT2-GW ...
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bioRxiv - Microbiology 2019Quote: ... Specific PCR products were excised from 2% agarose gel and cloned into pMD19-T (TaKaRa) and then sequenced ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 2 μL of lysis buffer (0.2% Triton X-100, 4U of RNase inhibitor, Takara) per well ...
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bioRxiv - Bioengineering 2022Quote: ... The resin was then packed in a TALON 2 mL Disposable Gravity Column (Takara Bio), and the column was washed three times with 3 mL of washing buffer (50 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2019Quote: ... The entire PCR product was subjected to electrophoresis in 2% agarose gel (TAKARA, Shiga, Japan) under 120 V/h for 1 hour in Tris-borate-EDTA (NIPPON GENE ...
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bioRxiv - Systems Biology 2020Quote: ... 2 μL of 5X SMARTScribe RT buffer and 0.5 μL SMARTScribe reverse transcriptase (Takara, 639536) was added for performing reverse transcription ...
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bioRxiv - Genomics 2019Quote: ... We mixed 2 µg of total RNA with 1 µl 10 mM dNTPs (Clontech #639125) and 1 µl of 50 µM SMART_dT18VN primer (for a complete list of primer sequences ...
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bioRxiv - Plant Biology 2020Quote: ... Yeast transformation was performed according to the Yeastmaker Yeast Transformation System 2 User Manual (Clontech). The primers used are listed in Supplemental Table S2.
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bioRxiv - Plant Biology 2020Quote: ... Yeast transformation was performed according to the Yeastmaker Yeast Transformation System 2 User Manual (Clontech). More than 1 × 106 yeast clones were screened in synthetic defined (SD ...
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bioRxiv - Microbiology 2021Quote: ... The 25 μl PCR reaction contained 12.5 μL of 2× PrimeSTAR Max Premix (TaKaRa, R045A), 0.4 μM of each primer (Genewiz) ...
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bioRxiv - Biophysics 2021Quote: ... The solubilized protein was incubated with 2 mL TALON cobalt metal-affinity resin (Takara Bio) for 1 h ...
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bioRxiv - Molecular Biology 2019Quote: ... Second strand synthesis and PCR amplification was done using the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pmol ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 infected patients were confirmed using a RT-qPCR kit (TaKaRa, Dalian, China) as recommended by the China CDC.
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bioRxiv - Microbiology 2021Quote: ... Specific PCR products were excised from 2% agarose gel and cloned into pMD19-T (TaKaRa) and then sequenced ...
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bioRxiv - Plant Biology 2022Quote: We used the GAL4-based Matchmaker Gold Yeast 2-Hybrid System (Clontech, Mountain View, California) for all Y2H and Y3H assays ...
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bioRxiv - Plant Biology 2024Quote: ... 2 μg of purified total RNA was treated with RNase-free DNaseI (Takara, Dalian, China) to remove contaminated genomic DNA ...
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bioRxiv - Microbiology 2023Quote: ... 2×SYBR Green PCR Mastermix (RR820A) and reverse transcription kit (RR047A) were purchased from TAKARA company ...