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Citations for Takara Bio :
651 - 700 of 1429 citations for BFF 122 CAS 1152314 49 2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... while amplicons over 5 kb were produced using PrimeStar GXL polymerase (Takara Bio, San Jose, CA, #R050A) in a two-step amplification reaction ...
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bioRxiv - Microbiology 2024Quote: ... 5′ RACE was carried out using SMARTer RACE 5′/3′ Kit (Takara Bio USA, Mountain View, CA) to identify the transcription start site (TSS ...
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bioRxiv - Neuroscience 2020Quote: ... TetO/NGN2 gene expression was induced by Doxycycline (2 μg/mL; Clontech, Madison, WI) on day 0 ...
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bioRxiv - Immunology 2022Quote: ... 2 μl of the pre-RT-PCR2 (PrimeScript™ II Reverse Transcriptase, Takara Bio) mix was added to each well ...
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bioRxiv - Molecular Biology 2019Quote: ... 5.0 μL of 2×SYBR® Premix Ex Taq™ II (TaKaRa, Dalian, China), and 3 μL deionized water ...
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bioRxiv - Plant Biology 2021Quote: ... The Yeastmaker Yeast Transformation System 2 was used according to the manufacturer’s instructions (Clontech). pGBK-53 and pGADT were used as positive controls ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA was produced from 2 mg overall RNA with AMV reversed transcriptive enzyme (TaKaRa) as per the supplier’s instruction ...
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bioRxiv - Plant Biology 2021Quote: ... and c-DNA was prepared using 2 µg total RNA samples (Takara Bio, UK). The c-DNA samples were diluted ten times and an aliquot of 2 µl of each sample per reaction was used for qRT-PCR ...
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bioRxiv - Plant Biology 2021Quote: ... Transformants were selected on Synthetic Defined (SD) medium lacking Leu and Trp (−2) (Clontech). Three individual transformants were grown overnight in liquid SD (−2 ...
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bioRxiv - Plant Biology 2021Quote: ... Reactions were performed by mixing 2 μL 5x In-Fusion HD enzyme mix (Clontech), 100 ng of linearized vector ...
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bioRxiv - Microbiology 2020Quote: ... The 20 μl reaction mixture contained 10 μl 2×TB green Premix DimerEraser (Takara), 1 μl of each primer ...
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bioRxiv - Microbiology 2020Quote: ... specific primer sets for SARS-CoV-2 and PrimeSTAR GXL DNA polymerase (TaKaRa Bio). The 5’ termini of RNA were amplified by using the 5’RACE System for Rapid Amplification of cDNA Ends ...
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bioRxiv - Biochemistry 2021Quote: ... wt and mutants was induced by treatment with 2 μg/ml Doxycycline (631311, Clontech) for 24-48 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using PrimeScript RT Master Mix (CD201-2, TaKaRa, Otsu, Japan), and qPCR was performed using SYBR Premix Ex Taq II (RR820L ...
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bioRxiv - Immunology 2021Quote: ... The RNA of the SARS-CoV-2 was extracted for reverse transcription (TAKARA, Japan). The SARS-CoV-2 was quantitative analyzed with real time PCR by targeting S protein.
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bioRxiv - Cancer Biology 2022Quote: ... 2 µg of plasmids were diluted in 200 µL Xfect transfection reagent (631317, Takara). The mixture was incubated at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL of TB Green Premix Ex Taq 2 (TliRNaseH Plus; Takara, Dalian, China), 0.8 μL of each primer (Table 1) ...
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bioRxiv - Bioengineering 2022Quote: ... 1 µl purified cDNA was amplified with an Advantage HF 2 PCR kit (Takara) in a 25 µl reaction containing 0.5 µl forward primer (10 µM ...
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bioRxiv - Cell Biology 2022Quote: ... and the supernatant was incubated with 2 mL TALON Metal Affinity Resin (Takara Bio) for 1 h at 4 °C with rotation ...
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bioRxiv - Microbiology 2023Quote: ... followed by RT-PCR using PrimeScript One Step RT-PCR Kit Ver.2 (TaKaRa) with primers no ...
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bioRxiv - Neuroscience 2022Quote: ... The titer of AAVs was measured by using AAVproR titration kit ver.2 (Takara).
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bioRxiv - Plant Biology 2023Quote: ... The reaction medium contained 2 µL of 5× In-Fusion HD Enzyme Premix (Clontech); 1 µL of linearized pFL61[GFP] (∼100 ng) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μL of TB Green Premix Ex Taq 2 (TliRNaseH Plus) (Takara, Beijing, China), 0.8 μL of 10 μM bidirectional primers (Table 1) ...
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bioRxiv - Bioengineering 2023Quote: ... 37°C for 2 hours in a plate coated with Retronectin (Takara Bio, T100B) with an MOI of ∼5.0 for each lentivirus (dCas9 lentivirus at MOI ∼5.0 and gRNA-MCP-fusion effector lentivirus) ...
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bioRxiv - Genomics 2024Quote: ... the first PCR was performed using a Multiplex PCR Assay Kit Ver.2 (Takara) with MIG-seq primer set-1 developed by Suyama and Matsuki (2015) ...
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bioRxiv - Plant Biology 2020Quote: ... 10 AtCBLs prey vectors were co-transformed into the yeast strain AH109 following manufacturer’s instructions (Clontech, CA, USA). The empty vectors pTOOL27 and pTOOL28 were also co-transformed into yeast with each prey or bait plasmid respectively to confirm that bait does not autonomously activate the reporter genes in the absence of the prey protein.
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin RNA II kit (Clontech Laboratories, Mountain View, CA, USA). The quality and quantity of total RNA were measured by Nanodrop 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: The Y2H assays were performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech, Mountain View, CA, USA). The full-length and truncated coding regions of WL1 were amplified and cloned into the yeast expression vector pGADT7 (Clontech ...
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bioRxiv - Genomics 2020Quote: ... Images (TIFF format) were analyzed using automated microscopy image analysis software Cell Select (Takara Bio USA, CA, USA). The chip was stored in a chip holder at −80 °C overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... The WT or mutant sequences were then cloned into the lentiviral vector pLVX-Puro (Clontech, Mountain View, CA) for lentivirus production ...
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bioRxiv - Cell Biology 2019Quote: Chimeras 1-5 were first generated in pBluescript by In-Fusion cloning (Takara Bio, USA, Mountain View, CA) of inserts encoding different fragments of human Myo1e PCR-amplified from pEGFP-C1-myo1e-EcoR1-into the PCR-amplified segments of pBS-SpMyo1 vector using primers designed to replace selected SpMyo1 domain sequences with corresponding HsMyo1e sequences ...
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bioRxiv - Cell Biology 2019Quote: ... The amplified products were ligated using ClonTech In-Fusion HD Cloning kit (Takara Bio USA, Mountain View, CA) and the resultant pBS-HsMyo1e construct contained MYO1E CDS flanked by myo1+ 5’ and 3’ UTRs ...
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bioRxiv - Plant Biology 2019Quote: ... and converted to RNA-seq libraries using the Low input library prep kit (Clontech, Mountain View, CA, USA). Each library was diluted to 2 nM ...
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bioRxiv - Genomics 2021Quote: ... according to the technical manual for PrimeScriptTM First Strand cDNA Synthesis Kit (Takara Bio Inc., Mountain View, CA). Amplification of VH and VL antibody fragments was carried out according to the standard operating procedure (GenScript ...
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bioRxiv - Biochemistry 2021Quote: ... was used to remove two SapI restriction sites from vector pEGFP-C1 (Takara Bio USA, Mountain View, CA). A Woodchuck hepatitis virus (WHP ...
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bioRxiv - Microbiology 2020Quote: ... Pseudovirus-containing supernatants were collected 48 h later and concentrated using Lenti-X™ concentrator kit (Takara, CA). Pseudovirus neutralization assay was then performed by incubation of SARS-CoV-2 pseudovirus with 5 folds serially diluted antibody with starting concentration of 1 μg/ml for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was amplified using SMARTer® Universal Low Input RNA Kit for sequencing (Takara, Mountain View, CA, USA). Ribosomal RNA was depleted using RIboGone™-Mammalian rRNA removal kit (Takara ...
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bioRxiv - Systems Biology 2021Quote: ... Ribosomal RNA was depleted using the RiboGone – Mammalian Kit protocol (Cat #634847, Takara Bio USA, Mountain View, CA). The SMARTer Universal Low Input RNA Kit protocol v2 (Cat #634938 ...
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bioRxiv - Cell Biology 2020Quote: ... Counted cells were dispensed into microwells using the ICELL8 MSND (640000, Takara Bio USA, Mountain View, CA, USA) at the concentration of 25 cells/μl in 0.5× PBS and 1× Second Diluent (640202 ...
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bioRxiv - Cell Biology 2020Quote: ... We used the mixed buffer of PBS and fiducial mix (640202, Takara Bio USA, Mountain View, CA, USA) as the negative control wells ...
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bioRxiv - Cell Biology 2020Quote: ... RNA isolation and reverse transcription were performed as described in the STAR METHODS section “Ddi2 expression analysis in mouse embryos using qRT-PCR.” Constructs encoding the DDI2WT and DDI2PD proteins were cloned into p905 (gift from Pavlína Řezáčová, IOCB CAS, Prague) and pTreTight (Clontech) expression vectors.
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Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... HSV-tkSR39-T2A-sIL2 construct was cloned from HSV-tkSR39-GFP using In-Fusion cloning (Takara Bio, CA) after adding six C-terminal amino acids (EMGEAN ...
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bioRxiv - Microbiology 2022Quote: ... 2X ProteoGuard EDTA-free protease inhibitor cocktail (catalog no. 635673, Takara Bio USA, Inc., San Jose, CA, USA), and DNase and RNase-free RPI molecular grade water (catalog no ...
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bioRxiv - Plant Biology 2021Quote: Plasmids for Citrine or mNeon-Green endogenous tagging were assembled using In-Fusion (Clontech, Mountain View, CA, USA), in which Citrine or mNeonGreen genes ...
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bioRxiv - Neuroscience 2021Quote: ... filtered through Whatman 0.45 µm CA filter (GE #10462100) and concentrated using Lenti-X™ Concentrator (Takara #631232) according to producer datasheet (overnight incubation) ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... showing a viral titer of >106 IFU/ml as measured by Lenti-X GoStix (Takara, Mountain View, CA). To develop stably expressing OptoXR-EYFP cells ...
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bioRxiv - Molecular Biology 2022Quote: ... poly-A libraries were prepared using the SMARTer ThruPLEX DNA-seq Kit (R400675, Takara, Mountain View, CA, USA). Each library was normalized to 1.5 nM ...
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bioRxiv - Cell Biology 2022Quote: ... Titer of the lentiviruses was measured with a lentivirus titration kit (Takara Bio USA, San Jose, CA, USA). The same multiplicity of infection (MOI ...
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bioRxiv - Cell Biology 2022Quote: ... which was used to screen a Mate & Plate™ Library-Universal Mouse (Normalized) (Clontech, Mountainview, CA; Cat#: 630482) using the stringent protocol according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Single stranded cDNA from the total RNA was synthesized with a RT-PCR kit (Clontech, Mountain View, CA) according to the kit’s instructions ...