Labshake search
Citations for Takara Bio :
751 - 800 of 1429 citations for BFF 122 CAS 1152314 49 2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was amplified by 15 cycles of PCR using the Advantage 2 PCR Kit (Clontech) with template switching custom oligo (TableS6) ...
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bioRxiv - Cell Biology 2023Quote: ... Viral supernatants were collected and concentrated 20x using Retro-X concentraror (Takara Bio, PT5063-2), and stored at -80°C ...
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bioRxiv - Microbiology 2023Quote: Y2H assays were performed according to Yeastmaker™ Yeast Transformation System 2 User Manual (Clontech). The coding sequences corresponding to HCPro1 ...
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bioRxiv - Developmental Biology 2024Quote: ... The RT-qPCR amplifications were performed using 2× SYBY Premix Ex TapTM II (Takara, China) as described previously ...
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bioRxiv - Genetics 2024Quote: ... Second strand synthesis and PCR amplification was done by adding Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (Integrated DNA Technologies ...
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bioRxiv - Immunology 2021Quote: ... 1ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: The VZV thymidine kinase gene (ORF36) was inserted into the Lenti-X pLVX-Puro vector (Clontech, Mountain View, CA) with XhoI and EcoR I sites ...
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bioRxiv - Molecular Biology 2020Quote: ... ∼900 bp homology arms to Usp9x were amplified from mouse genomic DNA using PrimeStar GXL polymerase (Takara, CA, USA) and Gibson assembly primers with 21 nucleotide overlap to adjacent fragments ...
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bioRxiv - Genomics 2020Quote: ... using a Multi Sample Nano Dispenser (MSND, SMARTer™ ICELL8® Single-Cell System, Takara Bio USA, CA, USA). Chip wells were sealed using SmartChip Optical Imaging Film (Takara Bio USA ...
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bioRxiv - Genomics 2020Quote: ... and Living Colors DsRed Polyclonal Antibody (rabbit anti-E2-Crimson, Clontech [now Takara Bio USA], Mountain View, CA, USA). Sections were washed and stained with the secondary antibodies Streptavidin:Alexa Fluor 488 (Biolegend ...
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bioRxiv - Neuroscience 2019Quote: ... The gene was cloned into pGEM-HE vector through the In-Fusion HD Cloning system (Clontech, Mountain View, CA). Amino acid sequence was identical to that in VectorBase.
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bioRxiv - Genomics 2020Quote: ... 1ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: Cell death was assessed by the release of LDH in conditioned medium using LDH Cytotoxicity Detection Kit (TaKaRa, CA).
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bioRxiv - Plant Biology 2020Quote: Y2H screening was performed using Matchmaker Gold Yeast Two-Hybrid System (Takara Bio USA, Inc., Mountain View, US-CA). For bait AP_SAP11-like_PM19 or AP_SAP11-like_PM19Δ40-56 was cloned into pGBKT7 (TRP1 nutritional marker ...
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bioRxiv - Developmental Biology 2020Quote: ... 5’-RACE and 3’-RACE reactions were performed with the Smart RACE cDNA Amplification Kit (Clontech, Palo Alto, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Sequencing libraries were prepared from the amplified cDNA using SMART-Seq PLUS kits (Takara Bio USA, Mountain View, CA). Unique dual indexes were used on the amplified libraries to identify samples ...
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bioRxiv - Microbiology 2020Quote: ... or directly cloned into linearized CS-CA-MCS (VLT-ORF63a and VLT-ORF63b) using In-Fusion HD Cloning kit according to the manufacturer’s instruction (Clontech). The primer sets used for cDNA cloning into the CS-CA-MCS plasmid are listed in Supplementary Table 4 ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-seq Kit v2 – Pico Input mammalian (Takara Bio, CA, USA) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... RNAseq libraries were made using the SMARTer ultra-low RNA kit V3 (Takara Bio USA, Mountain View, CA, USA). and analysed individually (i.e. ...
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bioRxiv - Microbiology 2020Quote: ... The construction of a library was performed using a SMARTer Stranded RNA-Seq Kit (Clontech Lab Inc., CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The nNOS and iNOS genes were individually cloned into the pIRESpuro3 mammalian vector (Takara Bio USA, Mountain View, CA). An inverse polymerase chain reaction (PCR ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybridization (Y2H) assays were performed according to the manufacture’s procedure (Clontech Laboratories Inc., Palo Alto, CA, USA). For each isozyme ...
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bioRxiv - Microbiology 2021Quote: ... by seamless cloning according to the instruction of In-fusion® HD Cloning Kit (Clontech, Palo Alto, CA, USA). NLS was inserted into pcDNA3.1 using High-Efficient Ligation Reagent Ligation High (TOYOBO) ...
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bioRxiv - Microbiology 2021Quote: ... indexed cDNA libraries were prepared using the SMARTer Stranded RNA-Seq Kit (Takara Bio USA, Mountain View, CA, USA). Library preparation was performed according to the instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and then was transcribed to cDNA using the Clontech SMARTer PCR cDNA Synthesis Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... The resulting 6.3 kb product was purified and inserted into BbsI linearized pDestTol2pA2_ubi:p2A-EGFP using In-Fusion Cloning (Clontech, Mountainview, CA) and transformed into high efficiency 10-beta competent E.coli (New England BioLabs ...
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bioRxiv - Cell Biology 2019Quote: ... Each sub-cloning was done by using the In-Fusion PCR cloning kit (Clontech Laboratories, Mountain View, CA, USA). Plasmids were integrated at the lys1 gene locus ...
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bioRxiv - Cell Biology 2019Quote: ... Using the online In-Fusion™ HD (IF) cloning system primer design website (Takara Bio USA, Mountain View, CA), we made primer sets for TMEM163 SNP ...
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bioRxiv - Systems Biology 2019Quote: ... followed by self-circularization using the In-Fusion HD Cloning Kit (Cat# 639648, Clontech Laboratories, Mountain View, CA, USA).
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bioRxiv - Cell Biology 2021Quote: ... red fluorescent protein fused with the mitochondrial targeting sequence from cytochrome c oxidase subunit VIII (Clontech, Mountain View, CA) using Lipofectamine 2000 (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were produced by transient transfection of human epithelium kidney 293T Lenti-X cells (Clontech Laboratories, Mountain View, CA) using the JetPrime transfection kit (Polyplus Transfection Illkirch ...
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bioRxiv - Immunology 2021Quote: Cell death was assessed by the release of LDH in conditioned medium using LDH Cytotoxicity Detection Kit (TaKaRa, CA). IL-1β levels in conditioned media were measured by ELISA (eBiosciences ...
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bioRxiv - Biochemistry 2022Quote: The recombinant protein in the soluble fraction was then purified using TALON Metal Affinity Resin (Clontech Laboratories, CA, USA) per manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The location of T-DNA in mt2 was confirmed by using the GenomeWalker kit (Clontech Laboratories, Mountain View, CA) following the manufacturer’s recommended protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... and verified whether it has self-activation reaction through synthetic dropout (SD)-Ade-His-Leu-Trp (Clontech, CA, USA) with X-α-galactosidase (x-α-gal) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then reversely transcribed to synthesize the first strand of cDNA using SmartScribe Rtase (Clontech, Mountain View, CA, USA). Owing to the terminal transferase activity ...
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bioRxiv - Molecular Biology 2022Quote: ... red fluorescence protein (DsRed) and NK-NT or NKN1 fragments were cloned into pET6xHN-N Vector (Takara, CA, USA). HEK293T cells were cultured in FP medium (DMEM containing 10% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... The ORF of HvHsfA6a was amplified and fused to GAL4 BD in the yeast vector pGBKT7 (Clontech, CA, USA), and the resulting constructs was designated as pGBKT7:HvHsfA6a ...
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bioRxiv - Immunology 2024Quote: ... The isolated RNA was RNase III fragmented and adaptor-ligated using PrepX mRNA Library kit (Takara, Mountain View CA), then converted into cDNA using Superscript III reverse transcriptase (Lifetech ...
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bioRxiv - Cancer Biology 2024Quote: ... and the remaining RNA isolation was done using the NucleoSpin RNA II Kit (Clontech Laboratories, Palo Alto, CA; 740955.50). 1 μg RNA was transferred into the cDNA Ecodry Premix Kit prior to the quantitative PCR program being run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293/17 cell media were harvested and concentrated overnight with a Lenti-X concentrator (Clontech, Mountain View, CA) according to the manufacturer’s protocol and resuspended in 10-fold less media volume prior to concentration.
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bioRxiv - Plant Biology 2023Quote: The full length cDNA of CLC2 was firstly cloned into a pMDTM20 T-vector (Takara Bio, San Jose, CA). The CLC2 containing AIM1 deleteion and AIM2 mutation as well as LDS mutated ATG8h were generated respectively by a Mut Express® II Fast Mutagenesis Kit V2 (Vazyme ...
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bioRxiv - Cancer Biology 2023Quote: ... 1ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR fragments of AGB1.x were ligated into the pGBKT7 DNA BD vectors (Takara Bio USA, CA, USA) and AGGs into the pGADT7 AD vector (Takara Bio ...
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bioRxiv - Genomics 2024Quote: ... the RNA samples were reverse-transcribed to cDNA using the SMART-Seq HT Kit (Takara Bio USA, CA, USA) following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: Cell death was assessed by the release of LDH in conditioned medium using LDH Cytotoxicity Detection Kit (TaKaRa, CA). IL-1β levels in conditioned media were measured by an ELISA kit (eBiosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 2 μL of PBS including 0.2% Triton X-100 and 4U of RNase inhibitor (Takara) per well ...
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bioRxiv - Cancer Biology 2021Quote: Second strand synthesis and PCR amplification was done by adding the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pmol ...
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Relationship between True Digestibility of dietary Phosphorus and Gastrointestinal Bacteria of GoatsbioRxiv - Microbiology 2019Quote: ... Taq buffer 5 μL of 10×Ex (20 mmol/L Mg 2+;TaKaRa Inc., Dalian, China), template DNA 0.35 μg ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM MgCl2 and 2 μl of CIAP (Calf intestine AP, 30 U/μl: Takara#2250A). For the Endo H reactions ...