Labshake search
Citations for Takara Bio :
401 - 450 of 1429 citations for BFF 122 CAS 1152314 49 2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... according to the manufacturer’s user manual (Takara Bio USA, Inc. Mountain View, CA, USA). One hundred pg of amplified cDNA was tagmented and indexed for sequencing using a Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Genomics 2020Quote: ... Chip wells were sealed using SmartChip Optical Imaging Film (Takara Bio USA, CA, USA) and centrifuged at 300 X g for 5 min at 22 °C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... by In-fusion cloning technology (In-Fusion® HD Cloning Plus CE, Takara, CA). The Cerulean encoding region was then replaced by a stretch of cDNA encoding eGFP ...
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bioRxiv - Bioengineering 2019Quote: ... The pET Express & Purify kits (In-Fusion Ready) were from Clontech (Mountain View, CA). DEAE and Sephadex G-100 were purchased from Bio-Rad (Hercules ...
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bioRxiv - Immunology 2019Quote: ... Expression vectors are based on pEGFP-n1 or pEGFP-c1 (Clontech, Mountain View, CA). The VSV-G pseudotyping vector pMD2.G and the viral packaging vector psPAX2 were gifts from Didier Trono (Addgene plasmids #12259 and #12260) ...
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bioRxiv - Microbiology 2019Quote: ... 5’-RACE was performed with SMARTer RACE cDNA Amplification Kit (Clontech, Mountain View, CA). For ZK2B10 gene-specific lineage analysis ...
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bioRxiv - Biophysics 2019Quote: ... 300 ng of the plasmid encoding the green fluorescent protein (pEGFP, Clontech, CA, USA) and 12 μL of Lipofectamine/Plus reagent ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA plasmids were collected from the competent bacteria using MidiPrep (Clontech, Mountain View, CA).
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... gel purified and cloned into a pEGFP-C1 vector (Clontech, Mountain View, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... 15 nucleotide overlaps were included in primers for infusion cloning (Clontech, Mountain View, CA). Where this failed ...
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bioRxiv - Neuroscience 2020Quote: ... followed by cDNA amplification using the Clontech SMARTer kit (Takara Bio, Mountain View, CA).
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bioRxiv - Plant Biology 2021Quote: All yeast protocols were performed according to the Yeast Protocols Handbook (Clontech, Mountainview, CA) with minor modifications ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were tagged with Gfp (enhanced green fluorescent protein; Clontech, Mountain View, CA, USA) at their N-terminus unless noted otherwise ...
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bioRxiv - Cell Biology 2021Quote: ... The sections were immunolabeled with antibodies against GFP (Clontech Laboratories Inc, Mountain View, CA) at 1:25 diluted in PBS/1% fish skin gelatin ...
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bioRxiv - Cell Biology 2020Quote: ... into the ICELL8® 350v Chip (640019, Takara Bio USA, Mountain View, CA, USA). We used the mixed buffer of PBS and fiducial mix (640202 ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... The PCR product was ligated to pEGFP-C1 vector (Clontech Laboratories, Mountain View, CA), resulting in plasmid pEGFP-hUFL1 ...
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bioRxiv - Cell Biology 2021Quote: ... or the pZsGreen vector (cat. # 632446; Takara Bio USA, Inc., Mountain View, CA, USA). Transformed E ...
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bioRxiv - Biochemistry 2022Quote: ... was added at the N-termini of EGFP and mCherry (Clontech, Mountain View, CA). For intramolecular FRET experiments ...
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bioRxiv - Microbiology 2022Quote: ... The coding sequence of TgAP3β was cloned into pGADT7 (Clontech Laboratories, Mountain View, CA) and co-transformed into competent AH109 yeast cells with a plasmid encoding E ...
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bioRxiv - Plant Biology 2021Quote: ... followed by purification via His60 Ni Gravity Column Purification Kit (Clontech Laboratories, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: Lentiviruses were packaged in Lenti-X 293T cells (cat#632180, Clontech, Mountain View, CA) using a three-plasmid expression system (pLKO.1-shRNAs-puro ...
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bioRxiv - Plant Biology 2019Quote: ... Resulting PCR products were cloned into EcoRI/SalI-digested pBD-GAL4 (Clontech, CA, USA) using standard restriction ligation ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Libraries were prepared with SMARTer universal low input RNA kit (Takara, Mountain View, CA) following ribosome depletion ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... the full length LMP7 was constructed into the pCMV vector (Clontech, Fremont, CA, USA) with a Myc tag linked to the N terminal insert ...
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bioRxiv - Physiology 2021Quote: Plasmid DNA encoding enhanced green fluorescent (EGFP)-actin (Takara Bio USA, Mountain View, CA) facilitated exogenous gene expression in rodent kidneys ...
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bioRxiv - Immunology 2020Quote: All cloning was performed using In-Fusion cloning (Takara Biosciences, Mountain View, CA USA). Insert sequences were purchased from Sigma-Aldrich or Integrated DNA Technologies (Coralville ...
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bioRxiv - Neuroscience 2019Quote: ... The calcium phosphate-based CalPhos Mammalian Transfection Kit (631312, Clontech Laboratories, Mountain View, CA) was used (Harata et al. ...
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bioRxiv - Neuroscience 2021Quote: ... we used the SMART-Seq v4 kit (Takara Bio USA, Mountain View, CA, USA) according to manufacturer’s specifications ...
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bioRxiv - Microbiology 2019Quote: All yeast protocols were done according to the Yeast Protocols Handbook (Clontech, Mountainview, CA) with minor modifications ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplification was achieved using TaKaRa Ex Taq DNA Polymerase (ClonTech, Mountain View, CA). This amplified PCR product was then cloned into the pGEM-T Easy Vector using T4 ligase (Promega ...
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bioRxiv - Immunology 2020Quote: ... 20 μl of supernatant was titered using Lenti-X GoStix (Clontech, Mountain View, CA) per the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatant was incubated with equilibrated TALON Metal Affinity Resin (Takara Bio USA, Inc., CA) for 20 min on ice ...
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bioRxiv - Microbiology 2024Quote: ... Cloning was conducted using an In-Fusion HD cloning kit (Takara Bio, CA, USA), and the resulting plasmids were named pTVT7_YG1_S ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by PCR with the SeqAmp PCR kit (Takara Bio USA, San Diego, CA). Unlike TARGET-Seq ...
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bioRxiv - Immunology 2024Quote: ... urine and kidney were carried out using NucleoSpin tissue kit (Clontech, Mountain View, CA) according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... passed through 0.45µm filter and concentrated with RetroX Concentrator (Takara Bio USA, Mountainview, CA). Concentrated lentivirus was added to cells in the presence of 6 μg/ml polybrene (MilliporeSigma ...
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bioRxiv - Cancer Biology 2024Quote: ... passed through 0.45um filter and concentrated with LentiX Concentrator (Takara Bio USA, Mountainview, CA). Concentrated lentivirus was added to cells in the presence of 6 μg/ml polybrene (MilliporeSigma ...
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bioRxiv - Immunology 2022Quote: ... CD34+ cells were cultured overnight on RetroNectin-coated plates (Takara, T100B, Mountain View, CA) in X-VIVO 10 media (Lonza ...
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bioRxiv - Pathology 2023Quote: Yeast transformation was performed according to the manufacturer’s instructions (Clontech, Mountain View, CA, USA). Yeast cells (strain Y2H Gold ...
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bioRxiv - Cancer Biology 2023Quote: HEK 293T cell line (Lenti-X™ 293T, Takara Bio, San Jose, CA, USA) was cultured in DMEM High Glucose (DMEM-HG cat# SH30243.02 ...
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bioRxiv - Immunology 2023Quote: HA-tagged TRIM34ΔSPRY constructs were cloned in pQCXIP (TaKaRa Bio, San Jose, CA, #631516) by SbfI (New England BioLabs #R3642S ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and were applied to the SMART RACE kit (Takara Bio USA, Mountain View, CA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: All yeast work was performed according to the yeast protocols handbook (Clontech, Mountainview, CA). For Y2H screening ...
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bioRxiv - Cell Biology 2024Quote: ... Human CXCR4 was cloned into the pAcGFPm-N1 plasmid (Clontech Laboratories, Palo Alto, CA), as described (20).
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bioRxiv - Systems Biology 2024Quote: ... Mir-X miRNA qRT-PCR TB Green kit (Takara BIO, San Jose, CA, USA) was used for cDNA synthesis and miRNA expression analysis ...
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bioRxiv - Microbiology 2024Quote: Tissue samples were collected and total RNA was extracted using Trizol (Takara, CA, JPN) as previously described ...
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bioRxiv - Developmental Biology 2024Quote: ... Seventy-percent confluent Lenti-XTM 293T cells (Takara Bio USA, Inc., Mountain View, CA) were transfected with Hdac3 lentiviral plasmids (Hdac3 KO or empty vector control [EV])26 ...
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bioRxiv - Immunology 2021Quote: ... 2.5 mM dNTP and 2 U/μL of recombinant RNase inhibitor (Clontech) then spun down and frozen at –80 °C.
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bioRxiv - Developmental Biology 2020Quote: ... Yeast transformation was conducted with Yeast Transformation System 2 (Clontech NO.630439). All primers used were listed in supplementary Table 1.
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bioRxiv - Microbiology 2020Quote: ... 2 µl of dNTP mix (TaKaRa, 2.5 mM concentration, 200 µM final), 0.125 µl of HotStart ExTaq (TaKaRa ...