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Citations for Lonza :
3151 - 3200 of 4130 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were routinely tested for mycoplasma contamination using MycoAlert PLUS mycoplasma detection kit (Lonza, MD, USA) and molecularly characterised using an “in house” panel of cellular and molecular markers to check that cell lines have not been cross contaminated (every 3-6 month) ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lines were regularly tested and verified to be mycoplasma negative using MycoAlert PLUS Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were regularly tested for mycoplasma contamination using a biochemical test kit (#LT07-318, Lonza, Switzerland) and were free of mycoplasma contamination.
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bioRxiv - Cell Biology 2022Quote: ... 25 μg mRNA was transfected into 2.5 × 106 freshly thawed fibroblasts using Cell Line Nucleofector Kit V (Lonza) and AMAXA program X-001 following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Transient transfections of these dominant-negative Rab11 a and siRab11 a into mouse MΦs (RAW 264.7 cell line) were performed with Amaxa mouse macrophage nucleofector kit (VPA-1009, Lonza) and DharmaFECT 4 Transfection Reagent (T-2004-01 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell cultures were washed with Dulbecco’s phosphate buffered saline without calcium or magnesium (DPBS-; Lonza, Walkersville, MD, USA) and detached with 0.25% trypsin with 0.53 mM EDTA (ATCC ...
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bioRxiv - Cell Biology 2022Quote: ... expression constructs were transfected into cells using the Amaxa™ 4D-Nucleofector (10μg plasmid cDNA; Lonza, Basel, Switzerland) or JetPrime (2μg plasmid cDNA ...
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bioRxiv - Cell Biology 2022Quote: ... All cell lines were routinely tested for mycoplasma contamination by using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza).
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bioRxiv - Microbiology 2022Quote: ... Both HeLa M and 293T cells and their derivatives were maintained in Dulbecco’s modified Eagle medium (DMEM) (Lonza) supplemented with 10% of fetal calf serum (FBS ...
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bioRxiv - Systems Biology 2022Quote: Human Dermal blood microvascular endothelial cells (DMECs) isolated from a single donor were purchased from Lonza (CC-2183). DMECs were cultured in EGM2-MV (Lonza ...
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bioRxiv - Cell Biology 2022Quote: ... BUB-1 and CLS-2::GFP production was performed in 2 L SF9 cells in Insect-XPRESS (Lonza) medium (1×10^6 cells/mL) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and Jurkat cell lines tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza Cat #LT07-318).
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bioRxiv - Microbiology 2024Quote: ... Cells were then maintained in K-SFM (untreated) or switched to culture in Keratinocyte Basal Medium (KBM, Lonza) with CaCl2 added to a final concentration of 1.5 mM ...
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bioRxiv - Synthetic Biology 2024Quote: ... using SF Cell line 4D-Nucleofector X Kit L or X Kit S (Lonza, V4XC-2024, V4XC-2032) with the program CQ-104 ...
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bioRxiv - Cell Biology 2024Quote: ... These cells are mantained in 30% FBS/1% antibiotic/antimycotic /1% Non-essential AA/ EGM-2 media (Lonza) with Laminin521 coating (5 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were routinely monitored for mycoplasma and confirmed negative at multiple times during this study using MycoAlert (Lonza). All cells were grown at 37°C with 5% CO2 in a humidified incubator.
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bioRxiv - Immunology 2023Quote: ... 1×10e6 cells per guide were electroporated with the corresponding RNP complex using Lonza Electroporation Kit V (Lonza). After 48 h ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: ... and nucleofection was performed as per the manufacturer’s recommendations (Lonza, SG Cell Line 4D-Nucleofector X Kit S). Monoclonal isolation of the knockouts were performed by limiting dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... iMEPM cells were tested for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza Group Ltd, Basel, Switzerland). Packaged lentiviruses containing pLV[shRNA]-EGFP:T2A:Puro-U6>Scramble_shRNA (vectorID ...
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bioRxiv - Molecular Biology 2024Quote: ... were added to ABCs and EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ (Lonza, CC-3162) with iMatrix was added to HUVECs and then incubated in 37°C at 5% O2 ...
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bioRxiv - Neuroscience 2024Quote: ... we used the 4D-Nucleofector system and Amaxa P3 primary Cell 4D-Nucleofector X Kit S from Lonza. The electroporation buffer used was P3 primary cell Nucleofector Solution with Supplement 1 (Lonza) ...
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bioRxiv - Microbiology 2024Quote: ... Cell lines were confirmed to be mycoplasma-negative in a MycoAlert PLUS assay (Lonza catalog number LT07-710) performed by the Koch Institute High-Throughput Sciences Facility (Cambridge ...
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bioRxiv - Cancer Biology 2023Quote: K562 cells containing deletions in ALAS2 or FECH were created using a multi-guide strategy via nucleofection (Lonza) of Cas9/RNP complexes (Gene Knockout Kit v2 ...
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bioRxiv - Immunology 2024Quote: ... 1 - 2.5 × 106 Jurkat T cells were resuspended in EP buffer from the Nucleofector® Kit V (Lonza). Cells were combined with 1.5 μg of donor plasmid in a reaction volume of 100 μL and electroporated on the Amaxa® Nucleofector® II using pulse code X-005.
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bioRxiv - Immunology 2024Quote: ... All recovered cell fractions were rested overnight in EP-TICLE culture media consisting of X Vivo-15 (Lonza) supplemented with 2% KnockOut serum replacement (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... All cell lines used were confirmed to be negative for mycoplasma by annual testing using MycoAlert Plus (Lonza). Last testing was performed on 14 August 2019.
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bioRxiv - Immunology 2024Quote: ... 16.4µl P3 and 3.6µl Supplement Reaction solutions from P3 Primary Cell 4D X Kit S (Lonza V4XP-3032) were added ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lines were tested once a month for mycoplasma contamination using Mycoalert® Detection Kit (Lonza, Basel, Switzerland). MCF7 and MDA-468 cells were grown in RPMI 1640 medium (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... siRNA transfections were performed using the 4D-Nucleofector and the SE cell line 4D-Nucleofector X kits (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Parasites and host cells were regularly tested for Mycoplasma contamination using the Mycoalert detection kit (Lonza; Basel, Switzerland). For all assays ...
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bioRxiv - Molecular Biology 2023Quote: ... All cell lines were frequently tested for mycoplasma using MycoAlert Plus Mycoplasma Detection Kit (cat# LT07-218, Lonza).
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were periodically tested to confirm Mycoplasma negativity using the MycoAlert mycoplasma Detection Kit (Lonza, Visp, Switzerland). Detailed methods for additional cell lines derived from solid tumors and leukemia are provided in Supplementary Materials and Methods.
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bioRxiv - Immunology 2023Quote: Electroporation was performed to introduce siRNA into BMDCs using a mouse dendritic cell nucleofector kit (Lonza, Basel, Switzerland) and a Nucleofector 2b (Lonza) ...
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bioRxiv - Immunology 2022Quote: ... used for baculovirus preparation were maintained in Insect-XPRESSTM Protein-free Insect Cell Medium with L-Glutamine (Lonza) at 28°C under agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... All cell lines were routinely tested for mycoplasma contamination by the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Molecular Biology 2023Quote: ... The RNP mixture was added to cells resuspended in nucleofection solution along with 0.5 ug pmax-GFP (Lonza) and 1 uL electroporation enhancer (100 uM ...
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bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney 293 (HEK293) cells (ATCC; CRL-1573) were grown in high-glucose DMEM (Lonza; BE12-604F) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... The MPRA vector library was nucleofected into TPP macrophages (5µg vector into 5×106 cells) in 100μl nucleofection buffer (Human Macrophage Nucleofection Kit, Lonza) using a Nucleofector 2b (program Y-011) ...
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bioRxiv - Developmental Biology 2023Quote: ... or pcDNA3.1(-) as a control using the AMAXA SG Cell line kit (4D-Nucleofector program EO-100; Lonza). Cells were further cultivated in DMEM/F12 supplemented with 10% FBS (Sigma ...
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An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: ... cells were kept in culture for 96 hours in complete hepatocyte basal medium (cat. no. CC-3198, Lonza). Cells were then fixed in 4% paraformaldehyde (cat ...
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bioRxiv - Physiology 2023Quote: ... USA) were cultured in 5% fetal bovine serum containing endothelial cell growth medium (EGM-2; Lonza, Basel, Switzerland). Media was supplemented with heparin ...
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bioRxiv - Cell Biology 2023Quote: ... Two days after transfection of WT HeLa cells with pSpCas9(BB)-2A-GFP-ghMyo10 using Amaxa nucleofection (Lonza), GFP-positive cells were subjected to single-cell sorting into 96-well plates using a BD FACS cell sorter ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼2-4 million cells were electroporated using the T-020 method of the Nucleofector™ 2b device (Lonza). Immediately after electroporation ...
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bioRxiv - Genomics 2023Quote: 2 × 105 HCT116-Cas9 and iPSC-iCas9 were resuspended in 20 µl SE cell line nucleofection solution (Lonza) (HCT116-Cas9 ...
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bioRxiv - Molecular Biology 2023Quote: ... or Vero-hACE2-TMPRSS2 cells (Vero AT) (BEI NR-54970) in Eagle’s Minimum Essential Medium (Lonza, 12-125Q) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... The neutrophil pellet was then cleared of remaining red blood cells using ACK lysis buffer (Lonza #10-548E). For subsequent experiments ...
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bioRxiv - Genetics 2023Quote: ... The cell suspension was transferred to the nucleofection cuvete and nucleofection was performed on the 4D-Nucleofector (Lonza) on the program for hESC ...
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bioRxiv - Immunology 2023Quote: ... 24-well plate) at a concentration of 75,000 cells per well in Smooth muscle growth medium (SmGM) (Lonza) and left to adhere and spread for 90 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 6-well dishes were seeded with 1.0 × 106 Sf9 cells per well in 2.0 mL InsectExpress medium (Lonza). Cells were transfected with 20 mL of bacmid per well ...
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bioRxiv - Immunology 2023Quote: ... beads were removed on day 3 followed by electroporation using the Human T Cell Nucleofector™ Kit (Lonza) and the Amaxa Nucleofactor® 2b (Lonza ...