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Citations for Worthington Biochemical :
1 - 50 of 157 citations for Gentamicin Deuterated C Complex Pentaacetate Salt Contains d0 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... then followed by digestion solution contain collagenase type II (Worthington) and protease type XIV (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... The neocortices were isolated from pups on day 0-1 in cold Earle’s Balanced Salt Solution (EBSS) and incubated for 15 min at 37°C in EBSS containing 10 U/ml papain (Worthington), followed by gentle dissociation in PBS containing 1.5 mg/ml BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... in Earle’s balanced salt solution (EBSS) (Worthington). Samples were incubated in 300µL of above papain solution at 37°C with continuous shaking (500rpm ...
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bioRxiv - Bioengineering 2024Quote: ... in Earle’s balanced salt solution (EBSS) (Worthington). These tubes were then placed in a rotating incubator at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... type 4 in Hanks’ Balanced Salt Solution (Worthington; Lakewood, NJ, USA) followed by red blood cell lysis with ammonium-chloride-potassium lysing buffer (Lonza ...
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bioRxiv - Neuroscience 2022Quote: ... The papain (LK003176) and the Earle’s Balanced Salt Solution (EBSS) (LK003188) were purchased from Worthington Biochemical Corporation (Lakewood ...
Product cited:
Earle's balanced salt solution (EBSS) as supplied in the Papain Dissociation System.Cat# LK003188, 1 vi, $61.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... The supernatant was discarded before the cell pellet was resuspended in Earle’s balanced salt solution (EBSS; Worthington Biochemicals) that contained 9.5%v/v Ovomucoid Inhibitor and 5%v/v DNase (Worthington Biochemicals) ...
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bioRxiv - Developmental Biology 2024Quote: ... Each epithelium was then washed once with HBSS and resuspended in Earle’s Balanced Salt Solution (EBSS, Worthington, #LK003188) containing papain (20 U/mL ...
Product cited:
Earle's balanced salt solution (EBSS) as supplied in the Papain Dissociation System.Cat# LK003188, 1 vi, $61.00 Ask -
bioRxiv - Developmental Biology 2023Quote: ... dispase (0.2 U/ml) and DNAse (168 U/ml) dissolved in Earle’s Balanced Salt Solution (EBSS, Worthington Biochemical Corporation). Up to 6 retinae were put in 1.7 ml of dissociation solution ...
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bioRxiv - Developmental Biology 2021Quote: ... Approximately 40-60 wing discs were dissociated in 250 μL of Papain (20 U/mL) and DNase I (200 U/mL) in Earle’s Buffered Salt Solution (all from Worthington Biochemical), at 25°C for 40 minutes ...
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bioRxiv - Immunology 2023Quote: ... Tissue was then washed in Hanks Balanced Salt Solution with Ca2+ or Mg2+ briefly and then digested in 1mg/ml collagenase (Worthington) for 30 minutes at 37°C ...
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Sex-specific perturbations of neuronal development caused by mutations in the autism risk gene DDX3XbioRxiv - Neuroscience 2024Quote: ... Cortices from single embryos were isolated in ice-cold Hank’s balanced salt solution (HBSS) and then digested with pre-warmed papain containing DNase (Worthington, #LK003150) at 37°C and 5% CO2 for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampi were dissected in ice-cold Hank’s balanced salts solution (HBSS) and kept on ice until digestion with 0.2 μm-filtered 10 U/mL papain (Worthington Biochemical Corporation) in HBSS in a 37°C water bath for 20 min ...
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bioRxiv - Neuroscience 2020Quote: Hippocampi and/or cortices from embryonic day 18 Sprague-Dawley rats of either sex was dissected in ice-cold Hanks Balanced Salt Solution (HBSS) and digested using a papain dissociation system (Worthington Biochemical Corporation). The appropriate density of neurons was plated on glass-bottom culture dishes (MatTek ...
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bioRxiv - Neuroscience 2020Quote: Hippocampi and cortices from embryonic day 18 Sprague-Dawley rats of either sex were dissected in ice-cold Hanks Balanced Salt Solution (HBSS) and digested using a papain dissociation system (Worthington Biochemical Corporation). The appropriate density of neurons was plated on glass-bottom culture dishes (MatTek ...
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bioRxiv - Cancer Biology 2024Quote: ... Tissue pieces were then washed briefly in Hanks Balanced Salt Solution with Ca2+ or Mg2+ and then digested in 1mg/ml collagenase (Worthington Biochemical Corporation) for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were digested with 1:50 (m/m) lysyl endopeptidase C (Fujifilm) at 42 °C for 2 hours and 1:50 (m/m) trypsin (Worthington) at 37 °C overnight ...
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bioRxiv - Genetics 2023Quote: ... To digest the polypeptide 10 µL of Glu C enzyme (Worthington) at 100 µg/mL was added and the samples digested overnight at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... at 4°C overnight and then digested with collagenase type II (Worthington). The cardiomyocyte fraction was collected after differential plating for 70 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... muscle fibers were digested with collagenase (CLSAFA, Worthington; 1 h, 37 °C), followed by 100 μm and 40 μm cell filtration steps ...
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bioRxiv - Physiology 2022Quote: ... at 37 °C with 229 U/ml collagenase type II (Worthington Biochemical Corporation), and washed in Tyrode solution for enzyme removal ...
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bioRxiv - Cell Biology 2020Quote: ... after digestion by papain and DNase (Worthington for 10 min at 37°C). Transfections were performed using the Amaxa nucleofector system following the manufacturer’s manual ...
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bioRxiv - Immunology 2021Quote: ... Kidneys were digested at 37°C in 1 mg/ml collagenase IV (Worthington) in complete RPMI for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... after digestion by papain and DNase (Worthington for 10 min at 37°C). Transfections were performed using the Amaxa nucleofector system following the manufacturer’s manual ...
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bioRxiv - Neuroscience 2020Quote: ... retinae were dissociated at 37°C for 30min with papain (LK003160, Worthington, Lakewood, NJ). The same volume of Neurobasal medium (Thermo Fischer ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003160, 1 bx, $265.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... and digested for 16 hr at 37°C with sequencing grade trypsin (Worthington Biochemical) in a 1:100 trypsin to protein ratio ...
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bioRxiv - Cell Biology 2020Quote: Tissues were digested enzymatically for 30 minutes at 37°C with papain (Worthington, LS003126) 20 units/mL in Ames ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated at 37°C with OPC papain solution (Worthington Cat#LS003124) for 20 minutes with constant inversion to prevent tissue aggregation ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003124, 25 mg, $40.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... and enzymatically digested at 37 °C first using 1 mg/ml Collagenase IV (Worthington, US) in L15 for 25 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... and digested overnight at 37 °C with 1 µg of trypsin (Worthington, LS003740 TPCK-treated). Samples were then acidified with formic acid (1 % final concentration).
Product cited:
A chromatographically purified, diafiltered, lyophilized powder that has been treated with...Cat# LS003740, 100 mg, $112.00 Ask -
bioRxiv - Pharmacology and Toxicology 2024Quote: ... then by collagenase type II for ∼16 hours at 37°C (583 Units/ml Worthington Biochemical Corp. ...
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bioRxiv - Cell Biology 2022Quote: ... followed by collagenase type II for ∼16 hours at 37°C (583 Units/ml Worthington Biochemical Corp. ...
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bioRxiv - Biochemistry 2023Quote: ... the particles were incubated at 37°C with 1 μg/μL trypsin TPCK (Worthington-Biochem) for 2 h to induce the proteolysis of BSA and measure in the spectrofluorometer ...
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bioRxiv - Immunology 2021Quote: ... tissues were digested in Collagenase solution for 1 hour at 37°C (Worthington Biochemical, Lakewood, NJ). Cells were washed once and blocked by Fc Block (Clone ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... Ganglia were incubated at 37□C for 25 minutes in 1 mg/ml papain (LS003119; Worthington), followed by 25 minutes of incubation at 37□C in 3 mg/ml collagenase type 2 (LS004176 ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
bioRxiv - Molecular Biology 2024Quote: ... and incubated for 30 min at 37 °C in papain solution (10 units/mL, Worthington, LS003126) containing N-acetyl-L- cysteine (160 μg/ml ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... Tissue pieces were then placed in a pre-warmed (at 37 °C) solution of papain (Worthington) and incubated at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... finely chopped and incubated for 30 min at 37°C with 1 mg/ml papain (Worthington), 1 mM CaCl2 ...
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bioRxiv - Developmental Biology 2024Quote: ... dissection products were then transferred in 250µl of 37°C pre-heated papain/L15 solution (Worthington, REF ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... and the cells dissociated for 30 min at 37 °C with 0.2% collagenase type III (Worthington LS004182) in Hibernate-A medium (Thermo A1247501) ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2022Quote: ... Chopped samples were incubated at 37°C for 30 min in collagenase III (3 mg/ml; Worthington). Liver samples were excised and meshed into single-cell suspensions through 70 μm meshes ...
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bioRxiv - Physiology 2021Quote: ... followed by a 15-min incubation at 37°C in type II collagenase (1.0 mg/ml) (Worthington) in Mg-PSS ...
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bioRxiv - Immunology 2020Quote: Endoproteinase Gluc-C (Staphyloccoccus aureus Protease V8) and chymotrypsin were obtained from (Worthington Biochemical Corp., Lakewood, USA). The ultrapure milli-Q deionized water (MQ ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were pelleted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DNaseI (Worthington Papain system ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue was then transferred to 1 mL tubes containing papain solution pre-warmed to 37° C (Worthington Biochemical LS003119 ...
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bioRxiv - Cancer Biology 2023Quote: ... and digested at 37°C for 30 min with 1 mg/mL Collagenase I (Worthington Biochemical, LS004194), 3 mg/mL Dispase II (Roche ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Cells were pelted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DnaseI (Worthington Papain system ...
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bioRxiv - Immunology 2024Quote: ... Spleen and lymph nodes were digested for 25 min at 37 °C with Collagenase IV (Worthington biochemical) and DNase I (Roche Diagnostics) ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...