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Citations for Worthington Biochemical :
201 - 250 of 290 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... glial cell line-derived neurotrophic factor (GDNF) and fetal bovine serum (FBS) were purchased from Worthington Biochemical Corporation (Lakewood ...
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bioRxiv - Systems Biology 2021Quote: To prepare single cell suspensions biopsies were minced and digested using Collagenase II (Worthington Biochemical Corporation) and DNase (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... EBSS was removed and individual cells were dissociated from the tissue in 100 µl Papain (Worthington, #LK03178 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets were dispersed and incubated with Ovo-mucoid protease inhibitor (Worthington Biochemical, cat. no. LK003182) to inactivate residual papain activity by incubating on a rocker at 4 °C for 20 min ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Developmental Biology 2019Quote: ... Tissue was dissociated into a single-cell suspension using papain according to the manufacturer’s instructions (Worthington), and resuspended in cortico-spinal motor neuron (CSMN ...
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bioRxiv - Plant Biology 2020Quote: ... The unfertilized ovules of each cell marker line were treated with enzyme solution (1 % cellulase [Worthington, USA] ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then dissociated by tissue trituration and papain treatment [20U/ml papain (Worthington Biochemical corporation), and 0.2 mg/ml L-cysteine (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Single cell dissociation was performed by incubating tissue in papain (1 mg/ml; Worthington Biochemical Co) plus 1% DNaseI (10mg/ml in HBSS ...
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bioRxiv - Neuroscience 2022Quote: ... Single-cell suspensions were prepared by using Papain containing l-cysteine and EDTA (vial 2, Worthington), DNase I (vial 3 ...
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bioRxiv - Immunology 2023Quote: ... NP-40 cell lysates were incubated with 100 μg/mL of DNase I (Worthington Biochemical, LS002139) or 50 μg/mL of RNase A (Worthington Biochemical ...
Product cited:
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bioRxiv - Neuroscience 2023Quote: ... Cortices were then dissociated into single cells via incubation in 20 U/mL Papain/DNase (Worthington) for 15-20 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Cortical tissue was dissociated into cells using 200u/ml papain (cat # LS003126, Worthington, Columbus, Ohio, USA) and DNAse followed by trituration ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated for 7 min with Neurobasal media containing Ovomucoïde (15 mg/mL, Worthington), and manually triturated 10 times with a 1000 ml pipette in OptiMeM supplemented with D-Glucose (20mM) ...
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bioRxiv - Developmental Biology 2024Quote: Cerebral organoids were dissociated into a single-cell suspension using Worthington Papain System kit (Worthington, LK003150) according to the manufacturer’s instruction ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 5 minutes at RT in fetal calf serum complemented with DNase I (Worthington), followed by mechanical dissociation ...
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bioRxiv - Neuroscience 2022Quote: ... and the cells dissociated for 30 min at 37 °C with 0.2% collagenase type III (Worthington LS004182) in Hibernate-A medium (Thermo A1247501) ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... Cells were pelleted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DNaseI (Worthington Papain system ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pelted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DnaseI (Worthington Papain system ...
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bioRxiv - Immunology 2023Quote: ... the cells of the lymph nodes were dissociated with collagenase type IV (Worthington Biochemical Co., NJ, USA) for 20 min at 37°C ...
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bioRxiv - Genomics 2024Quote: ... Harvested tumors were minced and dissociated to single cell suspension using the Papain Dissociation System (Worthington #LK003150) following manufacturer’s protocol without the use of ovomucoid protease inhibitor ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... Cells were resuspended in a collagenase/dispase solution of 1 mg/mL collagenase type II (Worthington, LS004176) and 0.4 mg/mL neutral protease (Worthington LS02104 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2024Quote: ... Cells were incubated in a solution of 2% collagenase type II (Cat # LS004174, Worthington Biochem, Lakewood, USA), prepared in Hanks Balanced Salt Solution (HBSS ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... Cells were resuspended in a collagenase/dispase solution of 1 mg/mL collagenase type II (Worthington, LS004176) and 0.4 mg/mL neutral protease (Worthington LS02104 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... Cells were dissociated by enzyme digestion with 95 U/mL collagenase type II (Worthington, cat. no. LS004176) and 0.6 mg/mL pancreatin (Sigma–Aldrich ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from HCT116 wild-type or knockout cells and treated with DNase I (Worthington Biochemical) at 25 °C for 20 min ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was discarded before the cell pellet was resuspended in Earle’s balanced salt solution (EBSS; Worthington Biochemicals) that contained 9.5%v/v Ovomucoid Inhibitor and 5%v/v DNase (Worthington Biochemicals) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then DNAse treated with 4 ml dissagregation media (DMEM, 10% FBS, 100 μg/ml DNAse (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Organoids were dissociated into single cells using a 1:20 mixture of DNAse in PBS (Worthington Biochemical, LK003163) and TrypLE (Thermo Fisher ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... hCOs were dissociated into single cells using a 1:20 mixture of DNAse in PBS (Worthington Biochemical, LK003163) and TrypLE (Thermo Fisher ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... fresh tumors were dissociated into single-cell suspension by incubating with collagenase III (1 mg/ml, Worthington, LS004182) and DNase I (0.1 mg/ml ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Genetics 2020Quote: ... the cells were resuspended in EBSS containing 50μg/mL of DNase I and 2 mg/mL ovomucoid (Worthington, LK003150). 0.5 volume of EBSS ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... The cortices were dissected out and dissociated into single-cell suspensions with a papain dissociation system (Worthington Biochemical Corporation) and plated onto poly-L-lysine (Sigma Aldrich)-coated plates (0.1% (wt/vol) ...
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bioRxiv - Neuroscience 2021Quote: ... pH adjusted to 7.6] and mechanically dissociated to single cells after adding ovomucoid (Worthington, 0.7 mg per 5 mice) and DNAse (Worthington ...
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bioRxiv - Neuroscience 2020Quote: ... Individual capillary endothelial cells were isolated by enzymatic digestion with 0.5 mg/ml neutral protease (Worthington Biochemical Corp., USA) and 0.5 mg/ml elastase (Worthington ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were washed with the buffer and were incubated with 0.2 mg/mL DNase I (Worthington Biochemical, US) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Niche cells from the bone marrow fraction were isolated by digestion with 1 mg/mL STEMxyme1 (Worthington, Ref#LS004106) and 1 mg/mL Dispase 1 (ThermoFisher Scientific ...
Product cited:
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and...Cat# LS004106, 50 mg, $110.00 Ask -
bioRxiv - Neuroscience 2022Quote: Individual brain organoids were dissociated into a single-cell suspension using the Worthington Papain Dissociation System kit (Worthington Biochemical). A detailed description of the dissociation protocol is available at Protocol Exchange ...
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bioRxiv - Neuroscience 2022Quote: Individual brain organoids were dissociated into a single-cell suspension using the Worthington Papain Dissociation System kit (Worthington Biochemical). A detailed description of the dissociation protocol is available at Protocol Exchange ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×106 HCT116 cells were used per each immunoprecipitation (IP) reaction and 15 units of micrococcal nuclease (MNase, Worthington) was used to digest the chromatin for the ChIP reaction ...
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bioRxiv - Bioengineering 2023Quote: ... or in vivo transplantation using an adaptation of a well-established neural cell isolation method (Worthington 9035-81-1).71 As with our undifferentiated hiPSC cultures ...
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bioRxiv - Bioengineering 2023Quote: Cells were isolated from semitendinosus muscles of Belgian Blue cattle by collagenase digestion (CLSAFA, Worthington; 1 h; 37 °C), filtration with 100 μm cell strainers ...
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bioRxiv - Immunology 2023Quote: ... Cells were then DNase treated with 4 ml disaggregation media (DMEM, 10% FBS, 100 μg/ml DNASE I (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: Cortex of E12.5 and E14.5 embryos was dissected and dissociated into single cells using the Papain Dissociation System (#LK0031500, Worthington Biochemical). Individual collected cells were processed for scRNAseq using ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... Cells cultured on collagen and mineralized collagen were collected using collagenase type I (1mg/mL in PBS, Worthington Biochemical) and passed through a 40 µm cell strainer ...
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bioRxiv - Molecular Biology 2019Quote: ... of 0.1 focus-forming unit (FFU)/cell in M199 medium supplemented with 1 mg/mL porcine pancreatic trypsin (Worthington Biochemical). Cell culture medium was collected 24–36 h post infection ...
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bioRxiv - Cell Biology 2019Quote: ... A single cell suspension was made by digesting tissue for 30 minutes at 37°C in Collagenase type 2 solution (Worthington Biochemical ...
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bioRxiv - Neuroscience 2020Quote: ... NBS1- and NBS8-organoids were dissociated to a single cell suspension for 30 minutes using the Papain dissociation kit (Worthington) according to the manufactures’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... All viruses were titrated using 1:10 serial dilution in triplicate in LLCMK2 cells in the presence of 2μg/mL TPCK trypsin (Worthington Biomedical) for 72 hours and TCID50/mL was determined by the Reed-Meunch method based on hemagglutination of 0.5% washed chicken red blood cells (Lampire ...
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bioRxiv - Cell Biology 2021Quote: ... The hypothalamus and pre-optic area were then dissected from vibratome sections and dissociated into single cells using the Papin Dissociation Kit (Worthington) as previously described24 ...
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bioRxiv - Bioengineering 2022Quote: ... cells showed spontaneous beating and were dissociated on day 15 by incubation with 280 U/ml Collagenase (LS004174, Worthington, USA) and 40 U/ml DNase (LS006331 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask