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Citations for Worthington Biochemical :
401 - 450 of 451 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The tissue was transferred to 50 mL conical tubes with 5 mL of digestion solution pre-warmed to 37C and composed of 150 U/ml Collagenase type III (Worthington Biochemical, Freehold, NJ) and 50 µg/ml DNase I ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Immunology 2024Quote: ... and then incubated in 5 mL digestion buffer (basal media, 10 µM Y-27632, 600 U/mL Collagenase IV (Worthington cat. No. LS004189), 0.1 mg/mL DNAse I (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... spun down by centrifugation at 500 x g for 5 minutes and resuspended in 10 ml of digestion medium (MEM containing 112 U/mL type I collagenase; Worthington, cat. #CLS-1), 1.2 U/mL dispase (Gibco ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Microbiology 2020Quote: ... Cells were incubated at 37°C in serum-free cell culture medium (IMDM) supplemented with 1 µg/ml L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK) trypsin (Worthington-Biochemical). Cell supernatants were harvested at indicated time points post-infection ...
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bioRxiv - Plant Biology 2020Quote: ... A 5 to 7-day-old protonemal tissue culture was scraped from solid plates and added to a 466 mM mannitol solution with 2% Driselase and Cellulase (21 unit/mL, Worthington CEL). The mixture was gently agitated for 1 h at room temperature (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/mL of papain (Worthington Biochem.), 1 mM EDTA (PanReac AppliCham ...
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bioRxiv - Physiology 2022Quote: ... after intracardiac perfusion of Phosphate Buffer Saline followed by a solution of HBSS containing type II collagenase at 2 mg/ml (Worthington, USA). Specific diets described below were introduced the day of the first dimerizer injection and maintained until sacrifice.
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bioRxiv - Immunology 2021Quote: CHO cells stably expressing wild-type SARS-CoV-2 S were resuspended in wash buffer (PBS 1 % BSA, 2 mM EDTA) and treated with 10 µg/mL TPCK-trypsin (Worthington Biochem) for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: Unlabeled and Alexa Fluor 488 (AF488)-labeled T3DCD or T1L virions (2×1012 particles/ml) were digested with 200 μg/ml Nα-p-tosyl-L-lysine chloromethyl ketone-treated chymotrypsin (Worthington Biochemical) in a total volume of 100 μl for 20 min at 32°C [34] ...
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bioRxiv - Neuroscience 2020Quote: ... and the supernatant aspirated before the tissue was incubated in a collagenase solution containing 2 mg/mL collagenase type II (CLS2; Worthington Biochemical) in 5 mL 1X HBSS in a 37°C rocking water bath for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... with 2 mL pre-warmed digestion enzyme in ACSF solution (Papain, 25 unit/mL and DNase I, 55 unit/mL, Worthington Biochemical). For spinal dorsal horn neuronal atlas construction ...
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bioRxiv - Cell Biology 2024Quote: ... whole hearts from 28 wk-old ♂ WT and R14Δ/+ mice were cannulated and perfused with modified Krebs buffer containing 2 mg/ml collagenase (Worthington) for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclei from 2 X 108 Hela or 293T cells were digested with 20 U of micrococcal nuclease (Worthington Biochemical Corporation, LS004797) for 45 minutes ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004797, 15 ku, $94.00 Ask -
bioRxiv - Molecular Biology 2023Quote: ... WAT depots from Scd1fl/fl mice were minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... and hearts were perfused retrogradely for 15-18 min at 37°C with HEPES-buffer containing 100 mg/mL collagenase type 2 (Cat#9001-12-1, Worthington, USA) and digested ...
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bioRxiv - Cancer Biology 2024Quote: ... minced into 1-2 mm3 pieces and then placed into a digestion solution containing 0.5 mg mL-1 collagenase D (Worthington Biochemical Corporation), 0.01 mg mL-1 DNase I (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: Day 25 flushed bovine chimeric embryos were cut into small pieces and treated with 2 mg/ml papain (Worthington Biochemical, LS003119), 1 mg/ml DNase I (Roche ...
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bioRxiv - Molecular Biology 2022Quote: Calvarial osteoblasts were obtained from 3 to 5-day-old C57BL/6 mice by sequential enzyme digestion [1 mg/ml collagenase type II (Worthington Biochemical, Lakewood, NJ, USA) in Hanks’ balanced salt solution (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: Enzymatic digestion of lung tissue was performed by intratracheal instillation via a 20-gauge catheter of 1 mL of 5 mg/mL collagenase I (Worthington Biochemical Corp, Lakewood, NJ) and 0.25 mg/mL DNase I (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Lungs were digested by intratracheal instillation via a 20-gauge catheter of 1 mL of 5 mg/mL collagenase I (Worthington Biochemical Corp, Lakewood, NJ) and 0.25 mg/mL DNase I (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... The cardiac ventricles were cut into pieces of approximately 1–2 mm3 and dissociated by trituration in 0.05% collagenase type II (Worthington, Lakewood, NJ, USA) 8-10 times for 5 min each ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue from 2-3 hypothalamiwas pooled into 1-1.5 mL of HABG containing papain (20 u/mL, Worthington Biochemical, Lakewood, NJ, US). Tissue was broken into smaller pieces by pipetting 2-3 times with a P1000 pipet followed by a fire-polished glass pipette with a wide opening ...
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bioRxiv - Genomics 2020Quote: ... Mouse adult cardiocytes were obtained by digesting adult mouse hearts by retrograde perfusion of a solution of collagenase type 2 (Worthington, Lakewood, NJ) in calcium-free solutions ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/ml of papain (Worthington Biochem., USA), 1 mM EDTA (PanReac AppliChem ...
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bioRxiv - Bioengineering 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/ml of papain (Worthington Biochem., USA), 1 mM EDTA (PanReac AppliChem ...
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bioRxiv - Bioengineering 2021Quote: ... Muscles were minced to create a homogenous cell slurry and then incubated for 2 h at 37C in 0.2% collagenase (Worthington Biochemicals, Lakewood, NJ) in DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... ovarian cortex samples were digested in DMEM containing 25 mM HEPES and 2 mg/mL collagenase type III (Worthington Biochemical Corporation, LS004183) for 2 hours at 37°C with occasional swirling ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004183, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... The hippocampal neurons were then dissociated via incubation at 37°C for 30 minutes in 2% papain solution (Worthington; catalog no. LS003127). Following incubation ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003127, 1 gm, $595.00 Ask -
bioRxiv - Systems Biology 2022Quote: ... testes were placed in a 15 ml conical tube containing 2 ml of prewarmed (35 °C) collagenase solution (1 mg/ml type I collagenase (Worthington, cat. # LS004196) and 60 U/ml Turbo Nuclease (Nacalai ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2020Quote: ... the heart was perfused with the same solutions with 50 μM Ca2+ and collagenase type II (1 mg/mL, CLS-2, Worthington, NJ, USA) for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... and cut into rings (about 1 mm in length) that were subsequently incubated in 2 mg/ml collagenase type II (Worthington Biochemical Corporation) for 2 hr ...
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bioRxiv - Cell Biology 2023Quote: Tissue samples were dissected into small pieces and digested with 1-2 mg/ml (275 U/ml) collagenase type II (Worthington, Columbus, USA) in 30 ml Dulbecco’s minimal essential medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: The primary neuronal cells were isolated from the brain tissues of 1∼2-day-old SD pups using the papain dissociation system (Worthington Biochemical Corporation) as instructed by the manufacturer ...
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bioRxiv - Biophysics 2023Quote: ... Microsomes (1–2 mg/ml proteins) were digested at progressively increasing enzyme concentrations of TPCK-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ) or chymotrypsin (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... The bulb of each DRG was cut into 3mm sections and placed in 5mL of pre-warmed digestion enzyme containing 2 mg/mL of Stemxyme I (Worthington Biochemical, LS004106), 10ng/mL of recombinant human β-NGF (R&D Systems ...
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bioRxiv - Microbiology 2022Quote: ... of 0.01 and maintained in MEM containing 0.3% bovine serum albumin (MEM/BSA) and 1 μg/mL of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-trypsin (Worthington, Lakewood, NJ, USA). Supernatants were collected every 12 h and viral titers were measured using a plaque assay.
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bioRxiv - Bioengineering 2024Quote: ... the cusps were denuded of their endothelium before being minced and placed into an enzymatic mixture of 2 mg/mL collagenase III (Worthington Biochemical, Lakewood, NJ), 0.1 mg/mL hyaluronidase (Worthington Biochemical ...
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bioRxiv - Microbiology 2024Quote: ... cells were cultured in 24-well plates to ∼95% confluency and then infected in triplicate wells for each time point in Opti-MEM™ medium (Thermo Fischer Scientific, Waltham, MA) supplemented with 1 µg/ml L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK, Worthington Biochemicals, Lakewood, NJ). The starting multiplicity of infection (MOI ...
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Analysis of RyR2 distribution in HEK293 cells and mouse cardiac myocytes using 3D MINFLUX microscopybioRxiv - Physiology 2023Quote: ... Once fluids ran clear the perfusion was switched to an isolation buffer which included 2 mg/mL collagenase type II (Worthington Biochemical, catalog: LS004176) for 7 minutes at 2 mL/min ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Microbiology 2024Quote: ... 2 mL of Opti-AB containing 1 μg/mL N-p-tosyl-ʟ-phenylalanine chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals, NJ, USA) was added to each well ...
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bioRxiv - Cell Biology 2024Quote: ... Each leaflet was incubated for 20 min at 37 °C in 2 mg/mL type II collagenase (Worthington Biochemical Corp., Lakewood, NJ, USA) in cell culture medium (advanced Dulbecco’s modified Eagle’s medium (Ad DMEM ...
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bioRxiv - Cell Biology 2021Quote: ... Organoid tissue retained on the filter was then back-flushed with 25 ml of cold HBSS and this tissue was further disaggregated in 2 ml Collagenase II (Celase GMP, Worthington Biochemical Corporation, Lakewood, NJ) also containing 200 μg DNAse1 ...
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bioRxiv - Cell Biology 2021Quote: ... Each tissue was cut and minced with scissors and then placed in 15 ml conical tubes containing digestion buffer (2 ml DPBS and Collagenase II at 3 mg/ml; Worthington Biochemical, Lakewood, NJ, USA) for 40 min of incubation at 37°C with gentle shaking at 100 rpm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 12-to 16-week old) were euthanized and flexor digitorum brevis (FDB) muscles were dissected and incubated with 2 mg/mL collagenase (Worthington Biochemical Co., NJ, USA) in the HEPES-Krebs solution containing 2 mg/mL bovine serum albumin for 2-3 hours at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: Six corneas from 2 female and 1 male 129S6/SvEvTac mice of each age group (2, 6, and 11-month-old) were dissected and pooled in 600 µL of papain solution (CAT LK003178; Worthington Biochemical Corp., Lakewood, NJ) and incubated for 1.75 hours at 37 °C ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Microbiology 2024Quote: ... The viruses were propagated in MDCK cells in the presence of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ).
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bioRxiv - Neuroscience 2023Quote: ... and 1 mM Na-pyruvate) and dissociated them by adding an equal volume of 2 x Papain stock solution (Worthington Biochemical Corporation, Lakewood, USA) at 37°C for 20 minutes ...